细胞培养中癌变原理的研究：IV.金仓乳鼠肺恶性转化...

The proceeding of malignantly transformed rodent cells in vitro is often accompanied by regular variation of chromosome numbers and structures. In order to clarify the relationship between them, we obtained five clones C1-1-5-from BHL B4, cell line, and analysed their biological characteristics. Results showed a positive correlation between frequency of chromosome 15 monosomy, it is suggested that suppressive gene of malignant phenotype be located on chromosome 15.     

细胞培养中的癌变原理研究Ⅲ.丁酸诱导金仓鼠恶性转化细胞的表型逆转和染色体非随机改变

用2mmol/L丁酸长期处理金仓鼠恶性转化细胞,得到一个丁酸依赖型的逆转细胞系.逆转细胞在形态、核质比例、增殖速度、琼脂表面生长能力等生物学指标上均接近正常细胞;且细胞内cAMP水平也有较大幅度提高。遗传学分析结果表明,与金仓鼠细胞癌变相关的第15号染色体单体出现频率呈非随机改变,转化细胞为67％,逆转细胞仅占27％。提示丁酸对转化细胞的影响可能涉及生化物质的代谢和基因的调控。