白血病患者T淋巴细胞及免疫球蛋白检测价值评价

目的 探讨T淋巴细胞及免疫球蛋白在白血病患者血液检测中的价值.方法 选取2015年1月至2019年1月本院收治的45例初诊和12例治疗后缓解的急性白血病患者作为白血病组,另选取同期健康体检者60名作为健康组.两组患者均采用流式细胞仪,ELISE等方法进行静脉血检测,比较两组Th1及Th2细胞因子水平、CD3+,CD4+,CD8+及CD4+/CD8+占比和免疫球蛋白水平差异性.结果 白血病组患者Th1细胞中IL-2、IFN-γ表达低于健康组,缓解时患者Th1细胞中IL-2、IFN-γ表达高于初诊时,白血病组Th2细胞中IL-4、IL-10水平高于健康组(P<0.05),缓解时患者Th2细胞中IL-4、IL-10表达低于初诊时(P<0.05);初诊时白血病患者免疫球蛋白IgA、IgM高于健康组,IgG低于健康组(P<0.05),缓解时白血病患者免疫球蛋白IgG、IgM水平与健康组比较差异无统计学意义,IgA高于健康组(P<0.05);白血病组患者在初诊时T淋巴细胞CD3+、CD4+、CD4+/CD8+低于健康组,CD8+高于健康组(P<0.05),与初诊时比较,缓解时白血病患者T淋巴细胞CD3+、CD4+、CD4+/CD8升高,CD8+下降(P<0.05);白血病组患者外周血CD4+CD25+、CD4+CD25+high高于健康组(P<0.05),缓解时白血病患者外周血CD4+CD25+、CD4+CD25+high低于初诊时(P<0.05);白血病患者初诊时乳酸托氢酶(LDH)水平高于健康组(P<0.05),缓解时LDH水平低于初诊时(P<0.05).结论 急性白血病患者T淋巴细胞细胞及免疫球蛋白表达异常,其指标变化在急性白血病患者的治疗指导具有临床意义.     

白血病患者化疗后应用白介素-2治疗的临床观察

目的：探讨白血病患者化疗后应用白介素-2（IL-2）治疗的临床疗效.方法：将50例白血病患者随机分治疗组和对照组,治疗组化疗后加入IL-2 100万U/d×14 d,对照组单用化疗,监测两组治疗前、后T淋巴细胞亚群及化疗后患者的临床疗效、不良反应.结果：治疗组治疗2周后T淋巴细胞亚群水平明显高于治疗前及对照组化疗后（P＜0.05）.治疗组完全缓解（CR）率与总有效（OR）率与对照组相近,但粒细胞缺乏发生率、持续时间及感染发生率低于对照组（P＜0.05）.结论：应用IL-2对化疗后患者进行治疗可明显提高T淋巴细胞亚群水平,并可减少化疗后不良反应.     

初发急性白血病患者外周血T淋巴细胞亚群的表达探讨

目的探讨初发急性白血病患者外周血中相关T淋巴细胞亚群表达情况。方法选取2017年2月~2019年3月于我院诊治的34例初发急性白血病患者作为研究对象(试验组),选择同期于我院体检的34例健康体检者作为对照组。检测两组外周血中相关T淋巴细胞亚群指标,对两组CD~(3+)、CD~(4+)、CD~(8+)、CD~(4+)/CD~(8+)、IL-2、IFN-γ、IL-4、IL-10进行比较。结果试验组初发急性白血病患者CD~(3+)、CD~(4+)、CD~(4+)/CD~(8+)水平分别为(61.20±4.64)%、(30.53±3.70)%和(1.02±0.23),均明显低于对照组,差异具有统计学意义(P0.05);而试验组初发急性白血病患者CD8+水平为(44.14±3.50)%,明显高于对照组,差异具有统计学意义(P0.05);试验组初发急性白血病患者IL-2为(11.60±1.13)pg/m L、IFN-γ为(23.25±2.50)pg/m L,均明显低于对照组,差异具有统计学意义(P0.05)。试验组初发急性白血病患者IL-4、IL-10分别为(41.36±3.50)pg/m L和(34.50±2.75)pg/m L,均明显高于照组,差异具有统计学意义(P0.05)。结论初发急性白血病患者外周血中CD~(3+)、CD~(4+)、CD~(4+)/CD~(8+)、IL-2、IFN-γ表达下降,CD~(8+)、IL-4、IL-10表达提升。     

白血病患者血清IL-6、MCP-1、IL-22水平变化及临床意义

目的：探讨白血病患者血清白细胞介素-6(IL-6)、人巨噬细胞趋化蛋白-1(MCP-1)、白细胞介素-22(IL-22)水平变化及临床意义。方法：选取江西省人民医院2018年6月-2021年6月收治的67例白血病患者作为白血病组,另外选取同期接受体检的健康者96例作为健康组,比较两组血清IL-6、MCP-1、IL-22水平,分析IL-6、MCP-1、IL-22在不同类型白血病患者中的表达水平。结果：白血病组与健康组性别、年龄差异均无统计学意义（P>0.05）;白血病组IL-6、MCP-1、IL-22水平均显著高于健康组（P<0.05）;Pearson相关性分析得出,血清IL-6、MCP-1、IL-22水平呈现互为正相关（P<0.05）;白血病患者中,急性白血病组血清中IL-6、MCP-1、IL-22水平均显著高于慢性白血病组（P<0.05）,淋巴细胞白血病组血清中IL-6、IL-22水平均显著高于粒细胞白血病组（P<0.05）,但两组MCP-1差异无统计学意义（P>0.05）;不同疾病时期白血病患者中,血清IL-6、IL-22水平从高到低依次为复发白...     

中山医科大学学报1989年(10卷)目录索引

不孕 子宫内膜分泌不良不孕妇女内膜胞浆女性激素受体和相关血清激素水平关系探讨(凌玲等)(1):9 白血病 淋巴细胞白血病免疫分型观察(陈运贤等)(2):63 鼻咽癌 鼻咽癌病人血清抗EB病毒特异性DNA多聚酶活性研究(马建山等)(3):5鼻咽癌病人淋巴细胞产生IL-2、IL-2受体活性及放射治疗对其影响的初步研究(马志楷等)(4):30     

恶性肿瘤患者血清白细胞介素6水平的变化

白细胞介素 6 (IL- 6 )由多种细胞产生 ,与其受体 (IL- 6 R)结合后对免疫应答、骨髓造血及肿瘤生长等多方面产生调节作用。有研究显示 IL- 6是多发性骨髓瘤、B淋巴细胞性淋巴瘤发病和转归的重要分子 [1 ]。但关于 IL- 6在实体瘤和急性白血病中水平及意义如何 ,国内报道不多。我们观察了实体瘤和急性白血病患者血清中 IL- 6的水平变化 ,现报道如下。1　资料与方法1.1　观察对象 :4 2例恶性肿瘤 ,均为 2 0 0 0年 12月至 2 0 0 1年 3月住我院肿瘤科和血液科的患者 ,男 2 9例 ,女 13例 ,中位年龄 39岁。其中实体瘤 2 0例 :鼻咽癌 7例 ,肺癌…     

急性髓细胞白血病树突状细胞诱导T细胞对自体白血病细胞毒性作用的研究

目的　研究急性髓细胞白血病 (AML)树突状细胞 (DC)诱导 T细胞对自体白血病的细胞毒性作用。方法　分离 AML 患者外周血单个核细胞 (MNC) ,在含有 GM- CSF、IL- 4、TNF- α等刺激因子的培养体系中培养7～ 14 d,通过形态学和免疫表型鉴定 DC。通过反复冻融的方法获取白血病抗原肽 ,分别以白血病抗原肽及白血病抗原肽 - HSP90复合物方式脉冲 DC,再将 DC与自体 T淋巴细胞共同孵育 ,以乳酸脱氢酶的方法检测经不同抗原方式激活的自体 T淋巴细胞对白血病细胞的杀伤效应。结果　急性白血病患者外周血 MNC中培养出 DC为 (6～11)× 10 4 / 10 6 MNC,经两种抗原方式激活的自体 T淋巴细胞对自体白血病细胞的杀伤活性分别为 (6 2 .0 7±8.2 9) %和 (35 .33± 9.0 4 ) % ;体外培养的 AML 患者外周血 MNC可诱导分化出大量 DC。结论　单纯白血病抗原肽与白血病抗原肽 - HSP90复合物方式脉冲的 DC均能够激活自体白血病特异性 T淋巴细胞 ,但后者具有更强的激活能力。     

肺结节病患者外周血淋巴细胞IL-2生成及反应能力

观察16例肺结节病患者外周血淋巴细胞 IL-2产生能力及对 IL-2的反应性,结果显示:患者 IL-2产生能力明显降低。外源性 IL-2诱导增殖反应虽有降低趋势,但无统计学意义。外源性 IL-2可增强 PHA 诱导的淋巴细胞转化,提示患者细胞免疫功能可能低下,而外源性 IL-2可使之部分恢复。     

白血病患者IL-2活性水平的定量分析

采用依赖白细胞介导素2(IL-2)的 CTLL-2建株细胞作为检测系统,用人重组 IL-2作对照(参照 NCI 所定的 u/ml),对15例白血病初发期患者、6例缓解期患者和26例正常人的外周血单个核细胞用 PHA-M 刺激,定量测定其 IL-2产量。结果表明:(1)正常人群中 IL-2活性水平为359.6±37.7u/ml;(2)白血病初发期患者 IL-2活性水平为251.5±184u/ml,其中11例低于或接近正常水平,占73%;(3)白血病缓解期患者 IL-2活性水平高于正常人群和初发期患者,为521±316u/ml。以上结果对研究白血病患者的免疫状态和选用适当的治疗方案有实际意义。     

慢性肾衰血透患者的细胞免疫学研究

测定50例慢性肾衰血透患者外周血T淋巴细胞亚群,同时测定其中20例病人外周血淋巴细胞白细胞介素2(IL-2)产生能力及 IL-2受体活性。发现患者淋巴细胞亚群绝对数显著减少。透析时期<1年与>1年两组间无显著差异。患者的IL-2活性与IL-2受体活性与正常对照差异无显著性,说明慢性肾衰血透患者免疫功能低下与其淋巴细胞正群绝对数下降有关。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.