IL-2、IL-6及其受体与多发性骨髓瘤的研究

采用固相放射免疫分析法（ＲＩＡ）和双抗体夹心酶联免疫法（ＥＬＩＳＡ）,对多发性骨髓瘤（ＭＭ）患者进行血清白细胞介素２（ＩＬ－２）（ｓＩＬ－２Ｒ）,白细胞介素６（ＩＬ－６）,白细胞介素８受体（ｓＩＬ－６Ｒ）检测,并进行长期随访。结果ＭＭ患者血清ＩＬ０２、ｓＩＬ－２Ｒ和ＩＬ－６、ｓＨ－６Ｒ水平明显升高,ＩＬ－２与２微球蛋白＊β２－ＭＧ）呈负相关,ＩＬ－６,ｗＩＬ－８Ｒ与β－ＭＧ呈正相关,初诊时血清ｓＩ     

SLE病人PBMC分泌IL—6活性水平的研究

为了研究了ＳＬＥ病人ＰＢＭＣ分泌ＩＬ－６的能力，我们采用ＭＴＴ比色法，用ＩＬ－６依赖细胞株（７ＴＤ１）分别测定了１５例活动期，１５例非活动期ＳＬＥ病人和１５例正常对照者ＰＢＭＣ培养上清ＩＬ－６的活性水平。结果表明ＳＬＥ患者ＰＢＭＣ自发分泌ＩＬ－６的水平显著高于正常人（Ｐ〈０．０２或Ｐ〈０．００１），活动期患者高于非活动期患者（Ｐ〈０．００１）。ｐＨＡ－Ｍ刺激培养体系后，ＳＬＥ患者及正常人ＰＢＭＣ培     

甲状腺细胞中白细胞介素—6,γ—干扰素和肿瘤坏死因子—α？…

目的 研究白细胞介素－６（ＩＬ－６）、γ－干扰素（ＩＦＮ－γ）和肿瘤坏死因子－α（ＴＮＦ－α）基因在甲状腺上皮细胞（ＴＥＣ）中的转录。方法 采用原代甲状腺细胞培养技术和逆转录－聚合酶锭反应（ＲＴ－ＰＣＲ）定性分析方法。结果 正常,Ｇｒａｖｅｓ病（ＧＤ）和多结节笥甲状腺肿（ＭＮＧ）的ＴＥＣ 表达ＩＬ－６ｍＲＮＡ,但在ＴＮＦαｍＲＮＡ仅在ＴＥＣ中表达,而正常和病理甲状腺细胞均未转录ＩＦＮ－γｍＲＮＡ。     

病毒性肝炎和肝硬化患者IL－6、TNF－α的变化

为研究白细胞介素－６（ＩＬ－６）、肿瘤坏死因子（ＴＮＦ－α）在肝炎和肝硬化（ＬＣ）患者中的作用。检测了１５例正常人，１８例急性病毒性肝炎（ＡＨ），３７例慢性肝炎（ＣＨ），２０例ＬＣ患者血清及外周血单个核细胞（ＰＢＭＣ）的ＩＬ－６、ＴＮＦ－α水平。结果以ＣＨ患者ＩＬ－６、ＴＮＦ－α水平最高（血清及ＰＢＭＣ的ＩＬ－６水平分别为７２．１±３２．９４Ｕ／ｍｌ，１４０．７±３３．５Ｕ／ｍｌ；血清及ＰＢＭＣ的ＴＮＦ－α水平为３．９７±１．３８ｎｇ／ｍｌ，６．３５±１．４１ｎｇ／ｍｌ）。恢复期或稳定期ＴＮＦ－α和ＩＬ－６水平明显低于急性期或活动期（Ｐ＜０．０１）。ＣＨ患者肝组织学活动指数（ＨＡＩ）分数与ＰＢＭＣＩＬ－６和ＴＮＦ－α水平呈正相关（γ分别为０．８９，０．６８；Ｐ＜０．０５）。提示ＩＬ－６和ＴＮＦ－α是肝脏损害重要的炎症介质，介导肝细胞损害。     

动脉平滑肌细胞增生受白细胞介素影响的研究状况

动脉平滑肌细胞（ＡＳＭＣ）的增生受白细胞介素（ＩＬｓ）的影响。ＩＬ－２低浓度时可促进ＡＳＭＣ的增生，高浓度时可抑制ＡＳＭＣ的增生，呈双相效应。ＩＬ－１、ＩＬ－６可以促进ＡＳＭＣ的增殖，ＩＬ－８可以促进ＡＳＭＣ的迁移，ＩＬ－４可抑制ＡＳＭＣ增生。当然，ＩＬｓ之间，ＩＬｓ与生长因了及其他相关因素之间形成一个复杂的网络从而发挥作用。     

老年及老年前期急性心肌梗死病人血浆β内啡肽、精氨酸加压素的动态改变

观察２５例老年及老年前期急性心肌梗死（ＡＭＩ）病人血浆β内啡肽（β－ＥＰ）、精氨酸加压素（ＡＶＰ）在梗死后第１，２，３，４，５，６，７，１４，２１天的动态变化，并与２０例正常人作对照，结果显示：在ＡＭＩ时血浆β－ＥＰ水平各天均低于正常人（Ｐ＜０．０５），其变化可能与儿茶酚胺抑制垂体释放β－ＥＰ有关；血浆ＡＶＰ在第４、５天水平高于正常人（Ｐ＜０．０５），其升高可能是机体应激反应所致。在ＡＭＩ伴有合并症时血浆β－ＥＰ水平高于无合并症者（Ｐ＜０．０５），其升高可能参与ＡＭＩ时上述合并症的发生。     

精氨酸加压素促血管平滑肌细胞增殖效应及其对IL－6分泌的影响

我们以培养的ＳＤ大鼠血管平滑肌细胞（ＶＳＭＣｓ）为模型，动态观察了精氨酸加压素（ＡＶＰ）对ＶＳＭＣｓ增殖作用和白细胞介素－６（ＩＬ－６）分泌功能的影响，亦对ＩＬ－６与ＶＳＭＣｓ增殖效应的关系进行了研究，旨在探讨ＡＶＰ和ＩＬ－６在高血压发病中的意义。结果表明：（１）ＡＶＰ对培养大鼠ＶＳＭＣｓ具有明显促增殖效应，随剂量增加和时间延长其作用加强；（２）ＶＳＭＣｓ可自身分泌ＩＬ－６，ＡＶＰ对其分泌有明显的抑制作用，且作用时间愈长，抑制作用愈显著；（３）ＩＬ－６作用于ＶＳＭＣｓ可明显促进其ＤＮＡ合成。上述结果提示，ＡＶＰ与ＩＬ－６可能均参与了高血压的发病。     

消化系恶性肿瘤患者血清与腹水中细胞因子活性变化

目的研究消化系恶性肿瘤（ＤＭＴ）患者血清与腹水中内源性ＩＬ２,ＩＬ６,ＩＬ８,ＴＮＦα和ＩＦＮγ的生物学活性．方法应用ＥＬＩＳＡ法检测了１５例ＤＭＴ患者（肝癌１１例,胆总管癌１例,胰腺癌１例,胃癌１例,直肠癌１例）血清与腹水中５种细胞因子活性,并与６例肝硬变（ＬＣ）患者和８例正常成人进行了比较分析．结果ＤＭＴ患者血清ＩＬ２,ＩＬ６的生物学活性显著低于ＬＣ（Ｐ＜００５）;腹水中ＩＬ２,ＩＬ８活性显著低于ＬＣ组（Ｐ＜００１）,而ＩＬ６和ＩＦＮγ活性则高于ＬＣ组（Ｐ＜００１,００５）．ＤＭＴ患者血清中ＩＬ６,ＩＬ８活性明显高于正常成人组（Ｐ＜００５）;ＩＬ２,ＩＦＮγ则低于正常成人组,但缺乏显著性．肝癌血清和腹水中ＩＬ２活性显著高于非肝癌组（Ｐ＜００５）;而ＩＬ６活性则相对降低（Ｐ＜００５）．结论恶性肿瘤患者血清中ＩＬ２和ＩＦＮγ活性低于正常人,是ＤＭＴ患者抗肿瘤免疫功能缺陷的标志．ＩＬ６对于预测ＤＭＴ患者的预后具有重要的意义     

心肌梗塞再灌注中血浆白细胞介素6的动态变化和其单克隆抗？…

目的 探讨急性心肌梗塞（ＡＭＩ）心肌缺血再灌注过程中血浆白细胞介素６（ＩＬ－６）的动态变化及其意义和其单克隆抗全的保护作用。方法 （１）用ＥＬＩＳＡ方法和ＦＡＣＳＯＲＴ流式细胞仪测定２８例经链激酶（ＳＫ）或重组组织型纤溶酶原激活剂（ｔ－ＰＡ）溶栓治疗的ＡＭＩ患入院即刻,再灌注１,４．５,１０,２０小时务 ＩＬ－６水平和中性粒细胞表面粘附分子（ＣＤ１１ｂ）蛋白表达,（２）在兔的缺轿再灌注模型上检测     

类风湿关节炎患者血清肿瘤坏死因子白细胞介素—6的检测及其临 …

目的：探讨类风湿关节炎（ＲＡ）患者血清瘤坏死因子（ＴＮＦ）α、白细胞介素－６（ＩＬ－６）水平的变化及其临床意义。方法：应用酶联免疫吸附法（ＥＬＩＳＡ）检测ＲＡ患者治疗前后血清ＴＮＦα、ＩＬ－６水平，并与部分临床指标作相关性分析。结果：ＲＡ患者血清ＴＮＦα、ＩＬ－６水平明显高于正常对照组，且与部分临床指标呈正相关，重度活动期ＲＡ患者ＴＮＦα、ＩＬ－６水平明显高于轻、中度活动期，治疗后随着病情好转，患     

Anti-inflammatory cytokine profile in acute coronary syndromes: behavior of interleukin-10 in association with serum metalloproteinases and proinflammatory cytokines

BACKGROUND: The anti-inflammatory cytokine interleukin-10 (IL-10) downregulates the production of metalloproteinases (MMPs) and upregulates the production of their tissue inhibitors (TIMPs). The aim of this study was to assess the levels of IL-10 in patients with acute myocardial infarction (AMI) and unstable angina (UA), as well as to investigate the relationship of circulating IL-10 with the levels of MMPs (MMP-1, -2, -9), their tissue inhibitor (TIMP-1), pro-inflammatory cytokines (IL-6, tumor necrosis factor (TNF)-alpha) and serum lipids in the same patient population. METHODS: Serum MMP-1, -2, -9, TIMP-1, IL-6, TNF-alpha and IL-10 were measured by ELISA assays in 23 patients with AMI and 20 patients with UA after their hospital admission, as well as in 16 healthy controls subjects. The lipid profile was assessed by measuring the serum levels of total cholesterol, HDL-cholesterol, LDL-cholesterol and triglycerides. RESULTS: AMI patients exhibited significantly higher serum levels of IL-10 as compared with those of UA patients and healthy controls (both P=0.005). In contrast, there was no significant difference in IL-10 levels between UA patients and healthy controls. In AMI patients there was a statistically significant positive correlation of serum IL-10 with the levels of MMP-9 (rho=0.588, P=0.003), IL-6 (rho=0.502, P=0.015) and HDL-cholesterol (rho=0.697, P<0.001), as well as a significant negative correlation with the levels of triglycerides (rho=-0.417, P=0.048). CONCLUSIONS: Our results suggest that UA is associated with low serum activity of IL-10, while a significant elevation of this anti-inflammatory cytokine accompanies the peripheral immune responses of AMI. This observation indicates that different patterns of inflammatory reactions are implicated in the pathophysiology of two clinical conditions.     

Evidence for continuous stimulation of interleukin-6 production in Crohn's disease.

Increased serum concentrations of acute-phase proteins can be found in active inflammatory bowel disease. Because interleukin 6 (IL-6) is one of the main mediators of acute-phase protein synthesis by the liver, the serum concentrations of IL-6 and the acute-phase proteins C-reactive protein, alpha 1-antitrypsin, and alpha 1-acid glycoprotein were determined in 70 patients with Crohn's disease (CD) and 23 patients with ulcerative colitis (UC). Disease activities were determined by established clinical activity indices. Serum IL-6 concentrations were significantly (P less than 0.005) increased in patients with CD (mean +/- SEM, 6.8 +/- 0.9 U/mL) compared with patients with UC (mean, less than 4 U/mL) and healthy controls (mean, less than 4 U/mL). Of patients with CD, 68.5% had serum IL-6 concentrations of greater than or equal to 4 U/mL, compared with 21.7% of patients with UC and 0% of healthy controls. There was a tendency toward higher serum IL-6 concentrations in patients with active CD than in patients with inactive disease. However, these differences were not statistically significant. There was no correlation between IL-6 serum concentrations and clinical activity indices, possibly because of the short circulatory lifetime and rapid hepatic clearance of IL-6 from the portal venous blood. In contrast to serum IL-6, acute-phase proteins, which have a longer circulatory lifetime, were significantly correlated with clinical activity indices. Only the follow-up of individual patients with initially highly active disease showed a further increase in IL-6 levels during acute exacerbations of the inflammatory process. The results show that most patients with even moderately active CD have significantly increased serum concentrations of IL-6, most probably reflecting a continuous stimulation of IL-6-producing cells.     

Serum interleukin 6 levels become elevated in acute myocardial infarction.

We have examined serum interleukin 6 (IL-6) levels in 12 patients with acute myocardial infarction (AMI). IL-6 levels became elevated in all patients, following the rise of serum creatine kinase (CK) activity. Peak IL-6 levels showed a good correlation with peak serum C-reactive protein (CRP) levels, while there was no direct relationship between peak IL-6 levels and peak CK activity. IL-6 mRNA was not detected in unstimulated "quiescent" rat cardiocytes cultured in serum-free medium, but its expression was induced by exposure of the cells to serum or ionomycin. These results show that IL-6 is synthesized in the myocardium and serum IL-6 levels become elevated in AMI, suggesting that IL-6 could affect the progression and/or healing processes of AMI.     

Cytokines in patients with ischaemic heart disease or myocardial infarction

BACKGROUND: Cytokines are responsible for the modulation of immunological and inflammatory processes as well as proliferative responses and apoptosis. It has been recently suggested that such cytokines as interleukin 6 (IL-6), soluble interleukin 6 receptor (sIL-6R) and anti-inflammatory cytokines such as interleukin 10 (IL-10) may play a significant role in the pathogenesis of acute coronary syndromes. AIM: To assess serum concentration of IL-6, sIL-6R and IL-10 in patients with ischaemic heart disease or acute myocardial infarction (AMI). METHODS: The study group consisted of 74 patients (25 females, 49 males, aged 40-69 years) divided into three groups; group I - 18 patients with AMI (up to 12 hours from the onset of symptoms), group II - 31 patients with unstable angina and group III - 25 patients with stable angina. The control group consisted of 20 healthy subjects. RESULTS: The IL-6 and sIL-6R serum levels were significantly higher in patients from groups I and II compared with patients from group III and controls, whereas the IL-10 serum concentration was similar in all studied groups. In patients with acute coronary syndromes serum concentrations of examined cytokines were positively correlated with acute inflammatory phase parameters and classical risk factors such as body mass index, blood pressure and lipid levels. CONCLUSIONS: IL-6 and sIL-6R are markers of acute coronary syndromes and may be used for the identification of high-risk patients with unstable angina or AMI.     

急性心肌梗死病人单核细胞中IL-6及TNF-α浓度及其临床意义

探讨急性心肌梗死病人单核细胞中IL-6及TNF-α的产生及其临床意义.方法 急性心肌梗死病人组(n=47)、正常对照组(n=13),于急性心肌梗死发病的第1,7,14及21天抽取外周血并分离单核细胞,通过酶联免疫荧光法测定单核细胞培养液中的IL-6及TNF-α浓度.结果 急性心肌梗死组单核细胞培养液中IL-6及TNF-α浓度较对照组明显升高;并在发病第1天前壁心肌梗死较下壁心肌梗死增高有显著意义;单核细胞培养液中IL-6浓度的峰值与血清C-反应蛋白浓度的峰值之间呈显著正相关关系.结论 单核细胞培养液中的IL-6及TNF-α对急性心肌梗死预后的判断有一定意义.     

急性心肌梗死血清白细胞介素-6、10水平与心功能研究

目的:观察急性心肌梗死(AMI)合并心力衰竭(心衰)患者血清白细胞介素(IL)-6、10对心功能的影响.方法:选择AMI患者(AMI组)30例于发病后24 h检测血清IL-6、IL-10.在心肌梗死后2周内观察心功能,心脏超声心动图检查测定左室射血分数(EF)、左室短轴缩短率(FS)、等容舒张期时间(IVRT).结果:AMI合并心衰组血清IL-6水平及IL-6/IL-10比值高于未合并心衰组,分别是IL-6:[(306.35±131.32)∶(198.59±52.08)ng/L,P<0.05]、IL-6/IL-10比值:[(18.18±12.66 )∶(11.49±7.47),P<0.05].相关分析:IL-6、IL-10与IVRT呈正相关(r=0.624、r=0.297,P<0.05),与EF(r=-0.559、r=-0.364,P<0.05)、FS(r=-0.453、r=-0.324,P<0.05)呈负相关,IL-6与心功能分级呈正相关(r=0.540、r=0.0.085,P<0.05).结论:AMI合并心衰时IL-6水平增高更为显著,IL-6/IL-10比值增大.     

冠心病与肿瘤坏死因子、白细胞介素-6浓度关系的研究

采用放射免疫法及酶联免疫吸附法检测35例冠心病患者（其中20例冠状动脉狭窄，15例心肌梗死）及20例健康者血清肿瘤坏死因子（TNF）、白细胞介素-6（IL－6）的浓度及血脂水平。结果显示：①冠心病组较对照组TNF浓度高（P＜0.01），而以心肌梗死组更为明显（P＜0.001）；②冠心病组TNF浓度与胆固醇、低密度脂蛋白-胆固醇、载脂蛋白B100呈正相关，而与高密度脂蛋白-胆固醇、载脂蛋白地呈负相关。③冠状动脉狭窄组与对照组比较IL－6浓度光显著性差异（P＞0．05），但心肌梗死组IL－6浓度显著增高（P＜0．001）。提示：TNF、IL－6与冠心病的发生发展有关，IL－6参与了急性心肌梗死的缺血损伤；内皮细胞受损，TNF、IL－6作用，脂质代谢的异常是冠心病发生的重要环节。     

Circulating interleukin-1 beta, interleukin-6, tumor necrosis factor-alpha, and soluble ICAM-1 in patients with chronic stable angina and myocardial infarction

The changes in serum concentrations of cytokines such as interleukin-1 (IL-1) beta, interleukin-6 (IL-6), tumor necrosis factor (TNF) alpha and a soluble-intercellular adhesion molecule (sICAM-1) has been investigated in patients with stable angina and acute myocardial infarction. Thirty-four patients with stable angina (SA), 15 with acute myocardial infarction (AMI), and 20 subjects in the control (C) group were included in the study. The mean serum concentrations of sICAM-1, IL-1-beta, IL-6, and TNF-alpha differed significantly among the three groups. Serum concentrations of IL-1 beta, sICAM-1, and TNF-alpha were comparable in the AMI and SA groups and higher than those found in the C group (p < 0.001). The serum concentration of IL-6 was more than twice as high in the AMI group as compared to the other two groups (p < 0.001). The mean serum concentrations of IL-1 beta, TNF-alpha, and IL-6 were comparable in the AMI and SA groups and higher than in the C group.     

急性冠脉综合征患者血清巨噬细胞集落刺激因子和白介素-10水平变化的临床意义

目的观察血清巨噬细胞集落刺激因子 (M-CSF)、白介素-10 (IL-10) 水平在急性冠脉综合征(ACS)患者中的变化,探讨其与斑块稳定性的关系.方法 ACS患者38 例(ACS组):急性心肌梗死(AMI)组20例,不稳定型心绞痛(UAP) 组18 例;稳定型心绞痛(SAP) 组17 例;对照组17 例.用ELISA法测定血清巨噬细胞集落刺激因子及白介素-10水平.结果血清M-CSF水平AMI组高于SAP组和对照组(P均<0.05),UAP组高与SAP组和对照组(P均<0.05),AMI组与UAP 组比较差异无显著性(P>0.05);血清IL-10水平AMI组高于UAP组、SAP组和对照组(P均<0.05), UA组高于SAP组和对照组(P均<0.05);ACS病人血清IL-10和M-CSF水平呈正相关关系(r=0.352,P<0.05).结论 (1)ACS患者血清M-CSF升高反映斑块的不稳定性.(2)急性冠脉综合征患者血清IL-10升高,并且与血清M-CSF水平呈正相关关系反映了在动脉粥样硬化斑块中作为抗炎因子的IL-10与其他炎症因子相互作用的复杂性.     

TNF-α和IL-6检测在急性冠脉综合征发病中的意义

目的　探讨急性冠脉综合征患者血清TNF -α和IL -6的浓度与发病的关系。方法　选择急性冠脉综合征 (急性心肌梗死及不稳定心绞痛 )患者 3 6例 ,其中急性心肌梗死 7例 ,不稳定心绞痛患者 2 9例。根据不稳定心绞痛患者病情的严重程度将其按Braunwald分级分为三组 :Ⅰ级 9例 ;Ⅱ级 8例 ;Ⅲ级 12例。采用放免法测定急性冠脉综合征患者血清TNF -α和IL -6的浓度。结果　不稳定心绞痛患者由Ⅰ级组到Ⅲ级组随病情加重IL -6的浓度是逐渐升高的(P <0 .0 5 ) ,但血清TNF -α无明显升高 ;急性心肌梗死患者血清IL -6浓度在心梗发作后 6小时和 48小时有两个高峰 ,血清TNF -α在心梗发作后 2 4小时达到高峰 ;IL -6和TNF -α与反映心肌损伤的酶CK -MB无直线相关性。结论　不稳定的心绞痛患者随着病情加重体内IL -6水平逐步升高 ;AMI患者发病过程中伴随IL -6和TNF -α的升高 ,但IL -6和TNF -α的动态曲线不同 ;IL -6和TNF -α浓度的升高与AMI的心肌损伤严重程度无关