Selective complexing of the "nuclear" 5S estradiol receptor by a serum component, 5S-CA.

A component present in several sera interacts selectively with the 5S "nuclear" form of estradiol receptor but not with the 4S "cytosol" form. This component is therefore referred to as "5S-complexing activity (5S-CA)." This selective interaction occurs with 5S receptor from mouse, calf, or rat. In addition, 5S-CA recognizes the nuclear form of the receptor from hypothalamic as well as uterine tissue Thus, 5S-CA indicates a general similarity in the chemical nature of 5S nuclear receptors prepared from several sources. Using 5S-CA as a chemical probe for the nuclear form of the estradiol receptor, I have shown the chemical as well as physical similarity of 5S receptors prepared in vivo (by injection of [3H]estradiol and subsequent isolation from nuclear/myofibrillar extracts) and in vitro (by labeling of cytoplasmic extracts and subsequent chromatography on columns of DNA-cellulose). These results indicate a distinctive chemical property of nuclear 5S forms of estradiol receptor. The data are interpreted with regard to models for 5S receptor formation. 5S-CA was found in sera from animals immunized against various antigens. The importance of testing for 5S-CA in antisera directed against steroid derivatives, steroid receptors, and other steroid-binding proteins is discussed.     

“5aG”毒株犬用抗狂犬病活疫苗的效果观察

1979年应用弱毒“5aG”毒株自制犬用抗狂犬病活疫苗。疫苗制备基本按长春生物制品研究所人用狂犬疫苗制备常规进行。免疫后6个月的血清抗体几何平均值为49.056;免疫后12个月为10.912。对6个公社的20,273只家犬进行免疫注射和一个对照公社观察结果:前者和后者的保护率分别为91.3％和89.4％,说明疫苗在一年内可使免疫注射的家犬产生抗狂犬病的能力。     

"Physiologic" aging in 5 years of pulmonary function in 140 iron miners

Ageing of the lung function in 5 years was studied in a group of 140 iron-ore miners, aged initially from 36 to 55 years. These men had no respiratory symptom and no pulmonary radiological abnormality. The sample was stratified on age and smoking. These factors were studied by a two-way analysis of variance. Lung function testing was performed twice, at 5 years of interval, in the same conditions. It consisted in: spirometry (for measuring FVC, FEV1, FEV1/FVC); a bronchial challenge test with acetylcholine (measurement of the fall of FEV1 after the test); measurement of total lung capacity (TLC) and residual volume (RV) by the open-circuit helium dilution method, and measurement of the transfer factor of the lung for CO, by the steady-state method (TCO/V). The only indices, whose values significantly changed in 5 years were: FVC, FEV1 and TCO/V. Their decrease rate did not appear to be significantly linked to age nor to smoking. According to these results, it seems that the decline of the pulmonary function is relatively constant during the middle period of life and that it is not strongly influenced by smoking, but only among subjects who remain without any respiratory symptom.     

Esophageal ulcer caused by the "pill"

Case report of a 24 years old woman with esophageal ulceration in relation to ingestion of oral anticoncipiens without fluid just before going to bed. Clinical symptoms were typically consisting of odynophagia and retrosternal burning. Endoscopy revealed rapidly healing of the lesion in the otherwise normal esophagus after short therapy with antacids. Symptoms are abolished definitively since flushing down the dragees with adequate quantities of water. This side-effect of the "pill" has not previously been reported.     

Calcium waves driven by "sensitization" wave-fronts

OBJECTIVE: Cellular Ca(2+) waves are understood as reaction-diffusion systems sustained by Ca(2+)-induced Ca(2+) release (CICR) from Ca(2+) stores. Given the recently discovered sensitization of Ca(2+) release channels (ryanodine receptors; RyRs) of the sarcoplasmic reticulum (SR) by luminal SR Ca(2+), waves could also be driven by RyR sensitization, mediated by SR overloading via Ca(2+) pump (SERCA), acting in tandem with CICR. METHODS: Confocal imaging of the Ca(2+) indicator fluo-3 was combined with UV-flash photolysis of caged compounds and the whole-cell configuration of the patch clamp technique to carry out these experiments in isolated guinea pig ventricular cardiomyocytes. RESULTS: Upon sudden slowing of the SERCA in cardiomyocytes with a photoreleased inhibitor, waves indeed decelerated immediately. No secondary changes of Ca(2+) signaling or SR Ca(2+) content due to SERCA inhibition were observed in the short time-frame of these experiments. CONCLUSIONS: Our findings are consistent with Ca(2+) loading resulting in a zone of RyR 'sensitization' traveling within the SR, but inconsistent with CICR as the predominant mechanism driving the Ca(2+) waves. This alternative mode of RyR activation is essential to fully conceptualize cardiac arrhythmias triggered by spontaneous Ca(2+) release.