艾灸对雷公藤甲素在佐剂性关节炎大鼠体内代谢的影响

1　实验材料1.1　实验动物 :健康实验用 Wistar雄性大鼠 ,体重为 2 0 0±2 0 g,由南京中医药大学实验动物中心提供。1.2　实验药物 :雷公藤甲素标准品及药用雷公藤甲素由中国科学院南京皮肤病研究所提供。1.3　实验仪器 :岛津 L C- 4A高效液相色谱仪 (日本岛津公司生产 )及天平等由南京中医药大学针灸研究所提供。2　实验方法2 .1　分组 :选 Wistar雄性大鼠 6 4只 ,随机分为 6组 (每组一笼群养 ,自由饮水、摄食 ,室温 2 2± 3℃ ) ,即对照组 8只 ,造模组 8只 ,治疗 A组 (雷公藤甲素灌胃 ) 12只 ,治疗 B组 (雷公藤甲素灌胃 ,于早上灌胃后…     

雷公藤多苷对大鼠佐剂性关节炎黏膜免疫的影响

目的观察雷公藤多苷对大鼠佐剂性关节炎(AA)黏膜免疫的影响。方法完全弗氏佐剂免疫大鼠,以雷公藤多苷治疗,观察大鼠黏膜免疫各项指标。结果雷公藤多苷可以改善AA大鼠病理改变,并调节AA大鼠的黏膜免疫功能。结论雷公藤多苷对AA模型大鼠有抗炎和免疫抑制作用。     

雷公藤多甙片对佐剂性关节炎大鼠的影响

目的研究雷公藤多甙片对佐剂性关节炎大鼠血清中丙二醛（MDA）含量的影响,从分子水平和抗氧化作用分析雷公藤多甙片对类风湿性关节炎的影响。方法选Wistar大鼠注射佐剂前后测量大鼠左后踝关节周径。每隔4d测其关节炎指数。给药28d后取血,采用测试盒测定各组大鼠血清中丙二醛（MDA）含量。结果雷公藤显著降低大鼠左足跖肿胀度及关节炎指数,显著降低血清中丙二醛（MDA）的含量,与泼尼松组相比显著降低血清中MDA的含量。结论雷公藤多甙片对Wistar大鼠无明显毒副作用,能显著抑制佐剂性关节炎的继发病变,降低血清中MDA的含量,其抗氧化作用治疗类风湿性关节炎的疗效优于泼尼松。     

历节合剂对佐剂性关节炎大鼠血液流变学的影响

目的 :观察历节合剂对佐剂性关节大鼠的血液流变学的影响。方法 :从实验方面研究 ,探讨了历节合剂治疗活动性类风湿性关节炎的疗效和机理 .实验结果表明历节合剂能明显抑制大鼠佐剂型关节炎的原发病变、继发病变 ,改善血液流变学的浓、粘、凝、聚状态。提示 :疏风清热、益气活血、解毒通络的治疗思路的可行性。     

痹痛定胶囊对大鼠佐剂性关节炎病变的影响

目的：研究痹痛定胶囊对大鼠佐剂性关节炎病变的影响。方法：选择Freund＇s完全佐剂复制佐剂性关节炎（AA）大鼠模型，观察痹痛定胶囊对大鼠右足跖肿胀度、大鼠血清PGE2、CRP值的影响。结果：痹痛定胶囊对大鼠足跖肿胀有明显的抑制作用，此作用较正清风痛宁强；能显著降低大鼠血清CRP、PGE2值，与正清风痛宁组比较，差异不显著（P〉0．05）。结论：痹痛定胶囊对关节炎具有治疗作用，其作用机理与抑制血清CRP、PGE2的产生有关。     

雷公藤内酯醇对佐剂性关节炎大鼠关节组织MCP-1表达变化的影响

类风湿性关节炎是临床上的常见病及多发病,若得不到及时诊治,其致残率极高。雷公藤内酯醇是雷公藤主要成分之一,其药理活性最高,在临床上用于治疗类风湿性关节炎,近期疗效很高。笔者以往的研究表明[1]雷公藤内酯醇可抑制MCP-1对佐剂性关节炎大鼠外周血T淋巴细胞的趋化作用。但雷公藤内酯醇对佐剂性关节炎大鼠关节组织细胞MCP-1表达变化的影响如何,尚未见文献报道。本研究旨在建立大鼠佐剂性关节炎模型基础上,通过应用不同剂量的雷公藤内酯醇,观察其对佐剂性关节炎病变组织细胞趋化因子MCP-1表达变化的影响,探讨雷公藤内酯醇治疗类风湿性…     

雷公藤内酯醇对佐剂性关节炎大鼠抗炎作用的实验研究

目的 观察雷公藤内酯醇对佐剂性关节炎(adjuvant arthritis,AA)大鼠的抗炎作用。方法 采用完全弗氏佐剂复制AA大鼠模型,并选取模型复制成功的大鼠随机分为5组:即正常对照组,模型组,雷公藤内酯醇低、中、高剂量组,分别给予生理盐水和100,200,300 μg·kg-1的雷公藤内酯醇,灌胃给药14 d。采用酶联免疫吸附法(ELISA)检测大鼠白介素1β(IL-1β)、肿瘤坏死因子(TNF-α)及魏氏法检测血沉水平,观察踝关节病理变化。结果 与模型组比较,雷公藤内酯醇组各剂量组大鼠血清IL-1β、TNF-α和血沉水平明显降低(P < 0.05,P < 0.01),下降水平呈明显的剂量相关性,其中雷公藤内酯醇高剂量组(300 μg·kg-1)降低最显著。结论 雷公藤内酯醇对AA具有良好治疗作用,作用机制与其下调AA大鼠外周血中1L-1β、TNF-α水平有关。     

雷公藤药酒对大鼠佐剂性关节炎治疗作用的研究

目的:观察雷公藤药酒对大鼠佐剂性关节炎(Adjuvant arthritis in rats,AA)动物模型的治疗作用。方法:用氟氏完全佐剂(Fluoride's complete adjuvant,FCA)诱导大鼠形成关节炎,经雷公藤药酒治疗后,观察大鼠足跖关节肿胀度,判断药效学结果。结果:雷公藤药酒能阻止大鼠继发性病变的发生,而治疗组能显著减轻大鼠足跖关节肿胀度。结论:雷公藤药酒不仅能改善大鼠佐剂性关节炎模型关节炎症状、抑制炎症反应,而且能预防继发性病变的出现。     

雷公藤对类风湿性关节炎的影响

雷公藤具有祛风湿,除痹痛,舒经活络的功效,在治疗风寒湿痹引起的类风湿性关节炎中,通过对炎症的对抗作用,对肾上腺皮质功能的影响,对免疫调节作用的影响,对关节滑膜成纤维细胞的影响,对血液流变学的影响几方面作用机制,使类风湿性关节炎之邪、虚、瘀得以治疗,直中病机.     

COMMENTS ON THE HOLISTIC ACTION OF ACUPUNCTURE IN VIEW OF ITS INFLUENCE ON NERVE-ENDOCRINE-IMMUNE NETWORK

In the present paper, the author makes some comments on the holistic action of acupuncture from the regulative effect on the nerve-endocrine-immune network, and the close relationship between the nerve-endocrineimmune network and the meridian-collateral system of TOM. The wholism concept of TOM refers to the organism being an integrated entirety, and the regulatory effect of acupuncture on functional activities of the organism relies on the integral connection of the acupoint--meridian-collateral-zangfu-organ system. When stimulated with acupuncture, the human body will brings its potential force into full play in preventing and treating diseases.     

仙龙颗粒对大鼠佐剂性关节炎T淋巴细胞亚群的影响

为探讨仙龙颗粒(XLG)对细胞免疫功能的影响,采用Freund's完全佐剂诱发SD大鼠佐剂性关节炎(AA),在灌服XLG 30天后,测定T淋巴细胞亚群CD 4、CD 8细胞表达率及CD 4/CD 8比值.结果显示XLG大剂量组明显抑制CD 4、提高CD 8细胞表达率,使CD 4/CD 8比值降低;中剂量组仅抑制CD 4细胞,对CD 8细胞无明显作用,CD 4/CD 8比值仍异常;小剂量组对CD 4/CD 8均无明显作用.表明XLG大剂量有较好的细胞免疫调节作用.     

INFLUENCE OF ACUPUNCTURE ON INSULIN RESISTANCE IN PATIENTS WITH SIMPLE OBESITY

With economical development of the society,elevation of livingstandard,andlife-style changes ,overweight andobesity are increasingly occurring,and their incidences tend to enhance year by year . Obesity not only affectsone’s posture , but also, more i mp…     

针灸治疗类风湿性关节炎对白细胞介素2影响的实验研究

<正> 类风湿性关节炎(简称RA)属祖国医学的“痹证”范畴,是以慢性对称性、多关节表现为主的一种全身性疾病。虽然病因尚不明了,但是认为其发病与自身免疫有关。近年来的研究表明RA与细胞免疫功能缺陷和体液免疫失调关系密切。为探讨     

EXPERIMENTAL STUDY ON THE EFFECT OF ACUPUNCTURE ON TROPISM-DISTRIBUTION OF TETRAMETHYLPYRAZINE AND THE CONTENT OF cAMP IN ARTHRITIS RATS

“The application of combined Acupuncture andmedication”is recorded earlyinNeijing(《内经》TheYellow Emperor’s Internal Classic),in which it isadvocated that medicineisinternally used and needlesexternally used,and its superiority has been con-firmed by a great number of clinical studies.To in-vestigate the mechanisms of acupuncture combinedwith medication,the authors of this paper observedthe influence of acupuncture on the distribution ofspecific medicine in rats,and found that acupunctu…     

CLINICAL STUDY ON ACUPUNCTURE IN THE TREATMENT OF POST-STROKE DEPRESSION WITH THE METHOD OF ACTIVATING THE DU MERIDIAN AND CLEARING THE MIND

Objective: To observe the therapeutic effect of acupuncture in the treatment of post-stroke depression (PSD) with the method of activating the Du Meridian and clearing the mind. Methods: Two hundred and thirty one cases of PSD were randomly divided into two groups, acupuncture and control groups. In acupuncture group, Baihui (百会 GV 20), Dazhui (大椎 GV 14), Neiguan (内关 PC 6), Taichong (太冲 LR 3) and Shenmen (神门 HT 7) were selected as the main points to invigorate flow of qi in the Du Meridian and clear the mind. Patients in the control group received Fluoxetine orally, 20 rng once per-day. In addition, the same routine treatments were given to both groups, strengthened with rehabilitation exercises as early as possible; 8 weeks constituted one therapeutic course. HAMD and severity of neuro-functional defect of each case were evaluated before and 8-week after the treatment. Results: The total effective rate of acupuncture group was 92.37%, the control group, 72.88%. According to x2 test, the difference between the two groups was remarkably significant (P＜0.05). Conclusion: Acupuncture therapy of activating the Du Meridian and clearing the mind in the treatment of PSD was safe and effective.     

OBSERVATION ON THE THERAPEUTIC EFFECT OF ACUPUNCTURE IN THE TREATMENT OF POSTOPERATIVE HEMIPLEGIA

Hemiplegiaisacommoncomplicationofcraniocerebraloperationinclinic.Acupuncturetherapycaneffectivelyraisethemyodynamiaoftheparalyzedlimbandaccelerateitsfunctionalrecovery[1 - 4 ] .FromJanuaryof 2 0 0 2toDecem berof 2 0 0 3 ,theauthorsofthepresentpapertreated 2 0casesofpostoperativehemiplegiapa tientswithacupuncturetherapyandachievedsatisfiedresults.Itisreportedasfollows.1　CLINICALDATAFortyhemiplegiapatientsundergoingcran iocerebraloperationwererandomlyandevenlydividedintocontrolgroupandtre…     

OBSERVATION OF THERAPEUTIC EFFECT ON TREATMENT OF THE GREATER OCCIPITAL NEURALGIA WITH ACUPUNCTURE

Thegreateroccipitalneuralgiaischieflymanifestedasprominentstabbing throbbingpainintheoccipitalr transmittedtothelateraltemproparietalregion.Thepainattackscontinuouslyorintermittently,associate paroxysmalaggravationofspinalnervedisorder.Thediseaseiscommonl…     

EXPERIMENTAL OBSERVATION ON ACUPUNCTURE TREATMENT OF MULTIPLE AORTO—ARTERITIS IN THE RABBIT

Ａｏｒｔｏ ａｒｔｅｒｉｔｉｓｉｓａｎｏｎｓｐｅｃｉｆｉｃａｒｔｅｒｉｔｉｓｉｎ ｖｏｌｖｉｎｇｔｈｅｍａｉｎｔｒｕｎｋｓｏｆｔｈｅｌａｒｇｅａｒｔｅｒｉｅｓ ,ｓｕｃｈａｓ ｐｕｌｍｏｎａｒｙａｒｔｅｒｙ ,ｅｔｃ..Ｔｈｅｒｅｈａｖｅｂｅｅｎｓｏｍｅｒｅｐｏｒｔｓａｂｏｕｔｔｈｅｔｒｅａｔｍｅｎｔｏｆｍｕｌｔｉ ｐｌｅａｏｒｔｏ ａｒｔｅｒｔｉｓｗｉｔｈａｃｕｐｕｎｃｔｕｒｅｉｎｊｏｕｒｎａｌｓ .Ｔｈｅｅｆｆｅｃｔｏｆａｃｕｐｕｎｃｔｕｒｅｔｈｅｒａｐｙｆｏｒａｏｒｔｏ ａｒ ｔｅｒｉｔｉｓｃｒｅａｔｅ…     

INFLUENCE OF ACUPUNCTURE ON INSULIN RESISTANCE IN OBESITY PATIENTS

Ｉｎｓｕｌｉｎｒｅｓｉｓｔａｎｃｅ (ＩＲ)ｉｓｒｅｆｅｒｒｅｄｔｏｄｅ ｃｒｅａｓｅｄｂｉｏｌｏｇｉｃａｌｅｆｆｅｃｔｏｆｉｎｓｕｌｉｎｃｏｍｐａｒｅｄｗｉｔｈｔｈｅｎｏｒｍａｌｃｏｎｄｉｔｉｏｎ .ＩＲｏｃｃｕｒｓｉｎｍａｎｙｋｉｎｄｓｏｆｄｉｓｅａｓｅａｎｄｕｎｄｅｒｌｉｅｓａｃｏｍｍｏｎｐａｔｈｏｇｅｎｅｓｉｓｉｎｓｏｍｅｄｉｓｅａｓｅｓ[1 ] .ＩＲｉｓｆｏｕｎｄｉｎｏｂｅｓｉｔｙｐａ ｔｉｅｎｔｓ,ｗｈｉｃｈｍａｋｅｓｏｂｅｓｉｔｙｂｅｃｌｏｓｅｌｙｒｅｌａｔｅｄｔｏｄｉａｂｅｔｅｓ,ｈｙｐｅｒ…