表皮郎格罕细胞表达高亲和力IgE-Fc受体

为了进一步证实抗Ｆ　ＲＩｍＡｂ特异性地结合至郎格罕细胞（ＬＣ）表面，我们用免疫金标记技术进行了免疫电镜检查。发现５ｎｍ金颗粒不连续性地分布于ＬＣ，而非其它表皮细胞表面，从而证明ＬＣ是抗Ｆｃ　ＲＩ反应性表皮细胞。最后，为了了解ＬＣ的Ｆｃ　ＲＩ基因表达与否，进行了ＰＣＲ试验。结果显示在富集ＬＣ而非去除ＬＣ的表皮细胞悬液中存在Ｆｃ　ＲＩａ、　和　链的转录物。总之，本研究提供了直接证据证明正常人表皮ＬＣ表达Ｆ　ＲＩ。     

带状疱疹患者T细胞亚群的检测

目的:检测带状疱疹患者外周血T细胞亚群表达水平。方法:采用流式细胞仪检测116例带状疱疹患者及80名健康对照者外周血T细胞亚群。结果:带状疱疹患者外周血CD3~+T细胞、CD4~+T细胞均显著高于健康人群,患者不同年龄组间、不同病程组间CD3~+T细胞、CD4~+T细胞无明显差异。结论:带状疱疹患者CD3~+T细胞、CD4~+T细胞升高,提示特异性细胞免疫得以重建。     

豚鼠体癣模型表皮郎格罕细胞的动态观察

应用ＡＴＰａｓｅ组化法对豚鼠实验性皮肤真菌病进行皮损表皮ＬＣ的动态观察。结果发现，在感染非治疗侧，ＬＣ受损，其密度明显降低，与正常对照例及感染治疗侧比较差异均有显著性意义。说明皮肤真菌感染后可造成皮损表皮ＬＣ的减少，从而引起一系列免疫病理变化。     

疥疮结节患者郎格罕细胞和淋巴细胞亚群的观察

疥疮结节患者郎格罕细胞和淋巴细胞亚群的观察吴安李伯埙谭升顺对疥疮结节患者的体液免疫已经进行了研究［１］。有人认为细胞免疫反应在其形成过程中也起着重要的作用［２］。为此我们应用了一组单克隆抗体对疥疮结节患者外周血及原位皮损进行了免疫组化标记（ＡＢＣ技术...     

免疫磁性分离技术分离,纯化小鼠表皮郎格罕细胞

为了探讨表皮郎格罕细胞（ＬＣ）的培养方法，研究其功能特点，须将ＬＣ与表皮其它细胞相分离。我们在Ｐｅｒｃｏｌｌ密度梯度离心技术纯化ＬＣ的基础上，采用免疫磁性分离技术，取得了更为满意的结果。通过生物素－亲合素的介导，用结合大鼠抗小鼠细胞表面Ｉａ抗原的单抗的免疫磁珠吸附ＬＣ表面，经高强度磁场筛选，从而将ＬＣ从表皮细胞悬液中分离、纯化出来。结果表明，ＬＣ的纯化率超过９９％，纯化后细胞活性超过９５％，产率超     

1,25（OH）2维生素D3对小鼠郎格罕细胞和接触变态反应的影响

研究了不同浓度的１，２５（ＯＨ）２维生素Ｄ（ＶＤ３）外涂对小鼠表皮郎格罕细胞（ＬＣ）的影响及对接触变态反应（ＣＨＳ）的局部和系统抑制作用。结果表明随着１，２５（ＯＨ））２ＶＤ３浓度的增加，ＬＣ树枝状减少，呈现卵圆或圆形细胞并且细胞数量下降。局部使用治疗剂量的１，２５（ＯＨ）２ＶＤ３能直接抑制小鼠的ＣＨＳ。并且，接受此种处理的小鼠脾细胞后，受体小鼠的ＣＨＳ亦同样抑制。这提示与诱导体内抑制性淋巴细胞的     

过敏性紫癜患儿T细胞亚群和免疫球蛋白检测

过敏性紫癜(Henoch-Schonleinpurpura,HSP)的基本病理改变是全身弥漫性小血管炎症反应,由HSP引起的肾损害是儿童时期常见的肾小球疾病,但其发病机理尚未明确。本研究应用多色流式细胞仪检测HSP患儿外周血CD3⁺、CD4⁺及CD8⁺细胞亚群的数量变化,并对血清免疫球蛋白和补体成份进行了测定,旨在探讨其发病机理。     

Precursors of Langerhans cells

LC are dendritic cells localized in the supra-basal layer of the epidermis and in mucous epithelia. Their density ranges from 200 to 900 cells/mm². Their first function is to take an antigen, process it and present the processed antigen to the lymphocytes ([1] Misery L. La cellule de Langerhans. Editions Techniques, Encycl. Méd. Chir. Dermatologie 1994;12-220-B-10). They are involved in numerous diseases. The origin of these cells has been debated but a consensus has now been reached. Langerhans cells (LC) were first described in 1868 by Paul Langerhans ([2] Über die Nerven der menschlichen Haut. Virch Arch A (Pathol Anat) 1868;44:325–337). He thought that it was a nerve cell. LC were first considered to be a component of the nervous system because their dendritic processes are similar so nerve fibers and because of their staining by gold chloride. Other authors have discussed a possible relationship with melanocytes. LC were regarded either as melanocytes, the progeny of melanocytes after division, or as melanocytes in an arrested stage of development ([3] Masson P. My conception of cellular naevi. Cancer 1951;4:9–15; [4] Fan J, Schoenfeld RJ, Hunter J. A study of the epidermal clear cells with special reference to their relationship to the cells of Langerhans. J Invest Dermatol 1959;32:445–450; [5] Zelickson AS. Granule formation in the Langerhans cell. J Invest Dermatol 1966;47:498–502). S100 protein is expressed on melanocytes, on nerve cells and on LC ([6] Cocchia D, Michetti F, Donato R. Immunochemical and immunocytochemical localization of S100 antigen in normal human skin. Nature 1981;294:85–87), but this does not imply that there is a common lineage. Subsequent research showed that this cell is in fact an immune cell. Electron microscopy has given us a new and very important insight into the nature of LC. Thus, there is no tonofilament, no desmosome and no melanosome ([7] Birbeck MS, Breathnach AS, Everall JD. An electron microscopy study of basal melanocyte and high level clear cell (Langerhans cell) in vitiligo. J Invest Dermatol 1961;37:51–63). The most important ultrastructural feature is the presence of Birbeck granules. Many experiments have pointed to a hone-marrow origin of LC and their probable relationship with the phagocytic mononuclear cell system.     

The inhibitory effect of PUVA on the immunity of experimental dermatophytosis in guinea pigs

Summary The effect of topical PUVA on the disease course and immunity of T. mentagrophytes dermatophytosis was investigated in guinea pigs. Animals which had been inoculated on nontreated skin showed mild erythematous lesions with scaling in a few days and then developed the most intense reaction between days 10 and 14. The lesions resolved completely by the third week. On the other hand, animals which had been inoculated on the PUVA-treated sites showed only mild squamous, erythematous lesions until the fourth postinfective week, when the intense reaction began to appear. Complete regression was observed by the fifth week in these animals. Trichophytin tests performed on the 14th day were positive in the guinea pigs of nontreated group, while negative in the PUVA-treated animals. The latter group revealed a positive reaction on the fifth week. PUVA did not show inhibitory effect on the sensitization by intracutaneous injection of trichophytin antigen. The PUVA treatment depleted the ATPase-positive Langerhans' cells. These results indicate that PUVA treatment suppresses the immunity of dermatophytosis and delays the spontaneous resolution of the lesions, and suggest that the Langerhans' cell is involved in the development of cell-mediated immunity in experimental dermatophytosis.     

Role of Langerhans cells in epidermotropism of T cells

Summary Certain T lymphocytes display a specific affinity for the epidermis (epidermotropism). Recent studies have suggested that Ia⁺ Langerhans cells (LCs) are possible targets for the epidermotropism. A variety of self-Ia-reactive cloned T cells were tested for their ability to migrate into the epidermis following intradermal inoculation into the footpads of syngeneic mice. Clone BB5 was chosen as representative of the epidermotropic T cells. We investigated whether the depletion of Ia⁺ LCs from the epidermis by tape-stripping could alter the migration of BB5 cells into the epidermis. The epidermal invasion of BB5 cells was markedly impaired in those mice whose LCs were depleted by 95% after repetitive tape-stripping. Because production of epidermal-derived thymocyte activating factor (ETAF) by the epidermal cells was augmented after repetitive tape-stripping, the diminished migration of BB5 cells into tape-stripped epidermis did not result from a decrease in ETAF production which is thought to attract T cells chemotactically. These results suggest that Ia⁺ LCs may play an inductive role in the preferential migration of T cells into the epidermis.     

The ATPase-staining of Langerhans cells in previously stored skin tissues.

The ATPase-staining of Langerhans cells is usually done on fresh, unfixed biopsy specimens. In the present study, we attempted to stain these cells in previously stored tissues of guinea pig skin. The ATPase activity disappeared after freezing or preservation in formalin or ethanol. In contrast, tissue specimens which had been stored in physiological saline at 4 degrees C stained as well as fresh cells even after 14 days.     

自体疣包埋治疗尖锐湿疣及其对机体T细胞亚群的影响

目的研究利用包皮环切术(PC)进行自体疣包埋治疗尖锐湿疣(CA)疗效及其对机体细胞免疫功能的影响。方法将102例包皮过长的尖锐湿疣患者随意分成两组,两组均采用PC微波治疗,治疗组在PC中进行自体疣包埋,对照组不进行自体疣包埋;两组治疗前后均检测外周血T淋巴细胞亚群。结果治疗组复发率低,疗效优于对照组,治疗组与对照组比较,CD8+细胞数量明显下降(P<0.05),CD4+细胞数量及CD4+/CD8+比值明显提高(P<0.05)。结论利用PC,自体疣包埋治疗尖锐湿疣疗效好,能降低复发率,其机理可能是自体疣组织埋植能改善CA患者的细胞免疫功能。     

Nail changes in Langerhans cell histiocytosis

Nail changes in Langerhans cell histiocytosis are distinctly uncommon. Paronychial erythema, swelling and subungual pustules of the fingernails and toenails were cardinal, and were supported by diffuse as well as dense collections of mononuclear Langerhans cells evidenced by microscopic investigation. Oral administration of co-trimoxazole (800 mg sulphamethoxazole + 160 mg trimethoprim) every 12 h, 50 mg/d cyclophosphamide and 80 mg/d predinisolone were the mainstay of treatment, supported by scalp tar shampoo and local betamethasone lotion application.     

银屑病患者皮损局部朗格汉斯细胞数量异常机制的研究

目的：探讨银屑病患者皮损局部朗格汉斯细胞(LCs)数量异常的原因。方法：培养银屑病患者皮损处角质形成细胞，通过微孔小室实验检测其上清液对单核细胞的趋化功能；通过酶联免疫吸附法(ELISA)检测上清液中单核细胞趋化蛋白—1(MCP-1)的表达。结果：银屑病患者皮损处角质形成细胞分泌上清液对单核细胞的趋化能力明显强于正常对照组；其分泌的MCP-1水平也高于正常人。结论：银屑病角质形成细胞表达的趋化因子趋化了更多数量的单核细胞至皮损局部，因此，银屑病患者皮损局部LCs的数量理应是增多的。但由于银屑病角质形成细胞表达的其它一些因子也促使了单核细胞衍生的LCs的活化，活化的LCs会迁移至淋巴结或活化后凋亡，又导致其数量减少。因此，银屑病患者皮损局部朗格汉斯细胞数量是一动态变化过程。