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1.
Kanazawa Y Saito Y Supriatna Y Tezuka H Kotani T Murata Y Okazawa H Ohnishi H Kinouchi Y Nojima Y Ohteki T Shimosegawa T Matozaki T 《Genes to cells : devoted to molecular & cellular mechanisms》2010,15(12):1189-1200
Mononuclear phagocytes such as dendritic cells (DCs) and macrophages in the lamina propria (LP) are thought to be important for both induction of inflammatory responses and maintenance of immunologic tolerance in the mammalian intestine. The molecular mechanisms by which these cells regulate intestinal immunity have remained poorly understood, however. Signal regulatory protein α (SIRPα) is a transmembrane protein that is specifically expressed in DCs, macrophages and neutrophils. Here, we show that SIRPα is abundant in CD11c(+) CD11b(+) LP cells of the mouse intestine. Whereas SIRPα did not appear to be important for the steady-state homeostasis of mucosal immunity in the intestine, the flagellin-stimulated production of IL-17 or interferon (IFN)-γ by LP cells of SIRPα mutant (MT) mice that lack the cytoplasmic region of the protein was markedly decreased compared with that observed with wild-type cells. Moreover, the flagellin-induced production of IL-6 by LP cells from SIRPα MT mice was also greatly reduced. SIRPα MT mice were also resistant to the development of colitis induced by IL-10 deficiency. Our data thus suggest that SIRPα expressed on CD11c(+) LP cells is important for the production of IL-17 or IFN-γ in the LP as well as for the development of colitis induced by IL-10 deficiency. 相似文献
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Reza Rahimian Mohammad Reza Zirak Nahid Fakhraei Ahmad Mohammadi-Farani Hanan Hamdi 《Immunopharmacology and immunotoxicology》2016,38(6):432-440
Inflammatory bowel disease (IBD) is a chronic inflammation of the gastrointestinal (GI) tract. Tropisetron, a selective 5-HT3 receptor antagonist, is highly used to counteract chemotherapy-induced emesis. Previous studies revealed the anti-in?ammatory properties of this drug. The aim of this study was to evaluate the role of peroxisome proliferator-activated receptor gamma (PPARγ) receptor in the protective effect of tropisetron in an animal model of ulcerative colitis. Experimental colitis was induced by a single intra-colonic instillation of 4% (V/V) acetic acid in male rats. Tropisetron (3?mg/kg) and GW9662 (PPARγ antagonist) (5?mg/kg) were given twice daily for 2 days after colitis induction. Forty-eight hours after induction of colitis, colon was removed and macroscopic and microscopic features were given. Moreover, colonic concentrations of malondialdehyde (MDA), nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) levels, myeloperoxidase (MPO), and PPARγ activity were assessed. Both macroscopic and histopathological features of colonic injury were markedly ameliorated by tropisetron. Likewise, levels of NO, MDA, TNF-α, and IL-1β diminished significantly (p?.05). GW9662 reversed the effect of tropisetron on these markers partially or completely. In addition, tropisetron increased the PPARγ and decreased the MPO activity (p?.05). Tropisetron exerts notable anti-in?ammatory effects in acetic acid-induced colitis in rats, which is probably mediated through PPARγ receptors. 相似文献
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T. G. Shishkina D. G. Sakharov N. N. Dygalo 《Bulletin of experimental biology and medicine》2001,131(3):263-264
Administration of specific oligonucleotide selectively inhibiting 2A-adrenoceptor gene expression into the locus coeruleus of male rats for 3 days activated the hypothalamic-pituitary-adrenal system, which was manifested in a rise of blood plasma corticosterone content in rats with normal and hypertrophied (after castration) adrenal glands. These data indicate that 2A-adrenoceptors of the locus coeruleus are involved in the regulation of basal plasma corticosterone content. 相似文献
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《Clinical microbiology and infection》2014,20(11):O894-O903
Since the first carbapenem-resistant Klebsiella pneumoniae strain was isolated in 2008, Enterobacteriaceae with reduced susceptibility to one or more carbapenems have emerged sporadically in different geographical regions in Croatia. These observations gave rise to a multicenter study on carbapenem resistance in Enterobacteriaceae from Croatia. Fifty-seven carbapenem-non-susceptible strains of Enterobacteriaceae were collected during 2011–2012 from four large hospital centres in Croatia. Overall, 36 strains produced VIM-1 β-lactamase, three produced NDM-1, and one produced KPC-2. A high degree of clonal relatedness was observed in Enterobacter cloacae and Citrobacter freundii strains, in contrast to K. pneumoniae strains. BlaVIM genes were located within class1 integron which contained genes encoding resistance to aminoglycosides (aacA4). The study found strong association between blaVIM and qnrB6 and between blaNDM and qnrA6 genes. 相似文献
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Mahya Najjari Gholamhassan Vaezi Vida Hojati Zahra Mousavi 《Immunopharmacology and immunotoxicology》2018,40(3):256-261
Purpose: Evidence show that statins possess wide beneficial cardioprotective and anti-inflammatory effects; therefore, in the present experiment, we investigated the antiarrhythmic properties of atorvastatin in ouabain-induced arrhythmia in isolated rat atria and the role of several inflammatory cytokines in this effect.Materials and methods: Male rats were pretreated with either of atorvastatin (10?mg/kg) or vehicle, orally once daily for 6 weeks. After induction of anesthesia, we isolated the atria and after incubation with ouabain, time of onset of arrhythmia and asystole as well as atrial beating rate and contractile force were recorded. We also measured the atrial levels of IL-1β, IL-6, and TNF-α after the injection of ouabain to animals.Results: Pretreatment with atorvastatin significantly delayed the onset of arrhythmia and asystole compared with vehicle-treated group (p?.01, p?.001, respectively). Incubation of ouabain boosted both atrial beating rate and contractile force in vehicle-treated group (p?.05), while these responses in atorvastatin-treated group were not significant (p?>?.05). Injection of ouabain elevated the atrial levels of IL-1β, IL-6, and TNF-α, while pretreatment of animals with atorvastatin could reverse the ouabain-induced increase in atrial IL-1β and IL-6 (p?.01 and p?.05, respectively).Conclusions: It is concluded that observed antiarrhythmic effects of atorvastatin might be attributed to modulation of some inflammatory cytokines, at least IL-1β and IL-6. 相似文献
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Neuroscience and Behavioral Physiology - Individual sensitivity to blockade of β1,2-adrenoceptors was studied by dividing rats into groups depending on the manifestation of conditioned reflex... 相似文献
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Prevalence of HBs antigen in blood donors in the Bouaké regional centre of blood transfusion in 2001
Kra O N'Dri N Ehui E Ouattara B Bissagnene E 《Bulletin de la Societe de pathologie exotique (1990)》2007,100(2):127-129
A prospective cross-sectional study was conducted in the regional center of blood transfusion in Bouaké from December 1, 2001 to February 28, 2002. One thousand two hundred thirty one new blood donors were tested. HBs Antigen detection was made according to ELISA technique (Hepanostika HBs Ag Uni-Form II). HBs Antigen prevalence in blood donors in Bouaké was 12.5%. One hundred fifty four blood donors were tested positive and were divided into 131 males (85%) and 23 females (15%). Their average age was 27, 5 years old (18-65 years). HBs Antigen carriage rate was lower in females and students. They were mainly pupils (62%) and had risk factors of hepatitis B infection (intramuscular injection, multiple sexual partners, unsafe sex). HBs Antigen carriage rate in blood donors is high in Bouaké and justifies the systematic screening of this Antigen in any blood donor to reduce the transfusion risk. On the other hand, it is necessary to modify the blood collection strategy in order to make the most of the donation and to decrease the residual risk. 相似文献
8.
Background: Poland is considered an ethnically homogeneous country, with no significant national, linguistic, religious or racial minorities. Thus, social differences in rates of maturation, height and weight may be assumed to contain a negligible genetic component and serve as a reflection of environment, i.e. living conditions.Aim: This study seeks to determine whether changes in economic conditions in Poland, in particular the acute economic crisis of 1977–1989 and the transformation of the political system in 1989, had an effect on the biological status of girls from various categories of the rural population.Subjects and methods: Rural girls aged 9.5–18.5 years were studied in 1967 (n?=?7889), 1977 (n?=?7771), 1987 (n?=?13?556) and in 2001 (n?=?9599). The stratification of participants (farmers, farm-workers and non-farmers) was based on the source of their family income, parents' education, number of children per family and household appliances. Age at menarche (AM), body height, and weight were used as biological indicators of living conditions.Results: During the decade 1967–1977, while a relatively good economic situation prevailed in the country, AM decreased by 0.64 years and distinct secular trends in height and weight were noted. During the decade 1977–1987, years of economic crisis, secular trends were arrested and AM increased by 0.11 years. Landless rural families were more strongly affected by food shortages than were farmers who were the food producers. The study, repeated in 2001, showed positive secular trends in body height and a decrease in AM of 0.24 years for decade for daughters of farmers this decrease in AM was twice as high as in non-farmer families. The latter group experienced acute unemployment after the political and economic system transformation (1989). AM was earliest in daughters of non-farmers, and latest in those from farmer families. In 1967, the difference between the mean ages at AM for these groups amounted to 0.53 years, in 1977 to 0.44 years, in 1987 to 0.33 years and to only 0.15 years in 2001.Conclusion: The categories of the rural population, farmers, farm-workers and landless rural inhabitants were variously affected by the economic crisis, as well as by the process of economic transformation. This shows that living conditions of each of those categories changed in different ways and to a different degree during the years 1967–2001. Farmers' families achieved the highest social advancement, as the AM of girls from those families decreased by 0.98 years compared to those from farmer-worker and landless rural families, which decreased by 0.85 and 0.60 years, respectively. 相似文献
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Natalia De ISIA C&#;line HUSELSTEIN Luc MARCHAL Marie-Nathalie SARDA-KOLOPP Jing-Ping OU YANG Jean-Franois STOLTZ Assia ELJAAFARI 《生物医学工程学杂志》2005,(Z1)
1 Introduction Mesenchymal stem cells (MSCs) are multipotential stem cells which can be expanded in culture while still maintaining their undifferentiated state. They have the potential to differentiate into distinct mesenchymal tissue cells, including chondrocytes. Thus, they are an attractive cell source for cartilage tissue engineering. In vitro high density micromass culture has been widely used for chondrogenesis induction. The objective of our study was to analyze viability and differe… 相似文献
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The purpose of the survey was the routine assessment of the MTBDRplus® kit performance in the determination and characterization of Mycobacterium tuberculosis resistance to rifampicin. The survey was carried out on a collection of 144 strains (126 of which were resistant to rifampicin) isolated on patients from 15 countries. Sensitivity to antituberculosis drugs was determined by a liquid culture system and the reference method was the amplification and sequencing of a target region of the rpoB gene whose mutations are responsible for rifampicin resistance (codons 507 to 533). The assessed kit was based on a reverse hybridization technique using eight overlapping probes covering the target region and four probes representing the most-frequently observed mutations. The assay performance was found excellent, specificity: 100%, sensitivity: 99.2%; 17 mutations affecting 10 codons were reported, two of which were newly identified. 相似文献
13.
How complex multipart visual objects are represented perceptually remains a subject of ongoing investigation. One source of evidence that has been used to shed light on this issue comes from the study of individuals who fail to integrate disparate parts of visual objects. This study reports a series of experiments that examine the ability of two such patients with this form of agnosia (integrative agnosia; IA), S.M. and C.R., to discriminate and categorize exemplars of a rich set of novel objects, “Fribbles”, whose visual similarity (number of shared parts) and category membership (shared overall shape) can be manipulated. Both patients performed increasingly poorly as the number of parts required for differentiating one Fribble from another increased. Both patients were also impaired at determining when two Fribbles belonged in the same category, a process that relies on abstracting spatial relations between parts. C.R., the less impaired of the two, but not S.M., eventually learned to categorize the Fribbles but required substantially more training than normal perceivers. S.M.'s failure is not attributable to a problem in learning to use a label for identification nor is it obviously attributable to a visual memory deficit. Rather, the findings indicate that, although the patients may be able to represent a small number of parts independently, in order to represent multipart images, the parts need to be integrated or chunked into a coherent whole. It is this integrative process that is impaired in IA and appears to play a critical role in the normal object recognition of complex images. 相似文献
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V. P. Zykov A. Yu. Shcherbina E. B. Novikova T. V. Shvabrina 《Neuroscience and behavioral physiology》2009,39(7):635-638
A total of 60 patients aged 6–16 years with tics and Tourette’s syndrome were studied. Antibodies to caudate nucleus proteins
were assayed by western blot hybridization. Ten patients with Tourette’s syndrome were found to have antibodies to caudate
nucleus protein. Seven patients with neuroleptic-resistant types of Tourette’s syndrome received single transfusions of immunoglobulin
preparations, which produced regression of vocal and motor hyperkinesias and improvement in behavior (remission for more than
six months). The observation of antibodies to caudate nucleus proteins and the positive effects seen on administration of
immunoglobulins to patients with Tourette’s syndrome support previous data on the possibility of using immunoglobulin therapy
in the treatment of tic-type hyperkinesias and provide evidence of the involvement of autoimmune mechanisms inducing damage
to the dopaminergic system of the striatum.
Translated from Zhurnal Nevrologii i Psikhiatrii imeni S. S. Korsakova, Vol. 108, No. 8, pp. 10–13, August, 2008. 相似文献
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Ekimova I. V. Guzeev M. A. Simonova V. V. Pastukhov Yu. F. 《Neuroscience and behavioral physiology》2021,51(6):704-710
Neuroscience and Behavioral Physiology - Objective. To assess changes in the time characteristics of the states of sleep and waking which may serve as nonmotor signs of the initial stage of PD... 相似文献
18.
《解剖学报》1988,(2)
Ju et al first parcellated in detail the bed nuclei of stria terminalis according to its cyto-and chemoarchitecture. They found that the oval nucleus (OV) was rich in neurotensin (NT)-and corticotropin-releasing factor (CRF)like immunoreactivities. Using indirect immunohistochemical technique it has been shown in the present study that both neuropeptides show quite similar distribution pattern in OV of the Sprague- 相似文献
19.
Antimicrobial resistance is a major health problem worldwide, but marked variations in the resistance profiles of bacterial pathogens are found between countries and in different patient settings. In Tunisia, the strikingly high prevalence of resistance of bacteria to penicillins and cephalorosporins drugs including fourth generation in clinical isolates of Gram negative bacteria has been reported. During 30 years, the emerging problem of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolates is substantial, and some unique enzymes have been found. Recently, evidence that Gram-negative bacteria are resistant to nearly all available antimicrobial agents, including carbapenems, have emerged. 相似文献
20.
XUE QIONG WU YANG LU JIAN QIN LIANG JUN XIAN ZHANG GUANG YU ZHANG CUI HUAN LU HONG MIN LI BEI CHUAN DING Tuberculosis Research Laboratory Tuberculosis Center the Second Affiliated Hospital Chinese PLA General Hospital Beijing P. R. China Thorax Disease Hospital of Hebei Province Shijiazhuang P. R. China The Research Institute of TB Control Prevention of Beijing Beijing P. R. China 《中华微生物学和免疫学杂志(英文版)》2006,4(1):1-8
The relationship between embB mutation of Mycobacterium tuberculosis and ethambutol (EMB) resistance of the clinical isolates of tuberculous patients in China was investigated by reverse dot blot hybridization (RDBH) in addition to evaluating the clinical value with application of PCR-RDBH technique to detect EMB resistance. In the present study, the genotypes of the 258 bp fragments of embB genes from 196 clinical isolates of M. tuberculosis were analysed with RDBH and DNA sequencing. It was demonstrated that 60 out of 91 phenotypically EMB-resistant isolates (65.9%) showed 5 types of missense mutations at codon 306 of embB gene, resulting in the replacement of the Met residue of the wild type strain with Val, Ile or Leu residues. In these mutations, the GTP mutation (38/91, 41.8%) and the ATA mutation (16/91, 17.6%) were the most encountered genotypes. The embB mutation at codon 306 could also be found in 69 isolates of phenotypically EMB-sensitive but resistant to other anti-tuberculous drugs, but no such gene mutation could be found in 36 strains of drug-sensitive isolates. Meanwhile, the concordance with the results of DNA sequencing for one wide-type probe and 5 probes for specific mutations was 100% . It was concluded that the EMB-resistance occurring in most M. tuberculosis is due to appearance of embB mutation at codon 306, and the PCR-RDBH assay was proved to be a rapid, simple and reliable method for the detection of gene mutations, which might be a good alternative for the drug-resistance screening. 相似文献