SLE患者外周血中CD4+CD25+CD127low/-标记的调节性T细胞与临床表现和实验室指标相关性研究

目的:探讨CD4+CD25+CD127low/-标记的调节性T细胞(Treg)在系统性红斑狼疮发病机制中的作用.方法:用流式细胞仪检测45例系统性红斑狼疮(SLE)患者和45例年龄、性别相匹配的健康志愿者外周血CD4+T细胞中Treg的百分比,同时分析SLE患者外周血中的CD4+CD25+CD127low/-标记的Tr...     

寻常型银屑病患者外周血CD4~+CD25~+及CD8~+CD25~+细胞的检测

目的研究进展期寻常型银屑病患者外周血CD4+CD25+和CD8+CD25+调节性T细胞的数量变化及其在银屑病免疫病理学发病机制中的作用。方法应用流式细胞术对进展期寻常型银屑病患者外周血CD4+CD25+和CD8+CD25+调节性T细胞进行检测。结果进展期寻常型银屑病外周血CD4+CD25+细胞及CD8+CD25+调节性T细胞数量与正常对照组相比,均显著降低(P<0.05,P<0.005),而CD4+CD25+/CD8+CD25+比值无显著性差异(P>0.05)。结论寻常型银屑病的发病与CD4+CD25+和CD8+CD25+调节性T细胞的同步降低有关,与二者的比值无关。     

散发型白癜风患者外周血CD4~+/CD8~+T细胞比值及CD4~+CD25~+T细胞的检测

目的检测散发型白癜风患者外周血CD4+/CD8+T细胞比值及CD4+CD25+调节性T细胞水平,探讨其与散发型白癜风发病的关系。方法散发型白癜风患者29例,男13例,女16例。通过流式细胞仪对散发型白癜风患者外周血CD4+/CD8+T细胞比值及CD4+CD25+调节性T细胞水平进行检测,并与20例健康人相比较。结果与健康对照组相比,散发型白癜风患者外周血中CD4+/CD8+T细胞比值的差异无统计学意义(P0.05),而CD4+CD25+调节性T细胞水平明显减少,差异有统计学意义(P0.05),但在不同病程的患者中CD4+CD25+调节性T细胞数量的差异无统计学意义(P0.05)。结论散发型白癜风患者外周血中存在CD4+CD25+调节性T细胞水平下降,可能与散发型白癜风的发生发展有一定关系。     

扁平苔藓患者外周血CD4+CD25+Treg细胞的检测

目的:检测扁平苔藓患者外周血CD4+CD25+调节性T细胞(regulatory T cells,Treg)的数量及FOX3 mRNA的表达.方法:采用流式细胞术对25例扁平苔藓患者和25例健康对照者外周血CD4+CD25+Treg细胞进行检测,并通过RT-PCR方法检测FOXP3转录因子在外周血单个核细胞的表达.结果:泛发性扁平苔藓和口腔扁平苔藓患者外周血CD4+CD25+ Treg细胞数分别为(2.68±2.21)%和(2.38±2.67)%,均低于健康对照者(7.87±1.77)%;扁平苔藓患者的FOPX3表达水平也低于健康对照(P<0.05).结论:CD4+CD25+ Treg细胞数量和功能下降,可能通过导致扁平苔藓患者免疫功能紊乱参与了扁平苔藓的发病.     

银屑病和白癜风患者外周血CD4+CD25+调节性T细胞的检测

目的: 检测CD4+CD25+调节性T细胞(Treg)在白癜风和银屑病患者外周血中的表达.方法: 用流式细胞术检测19例白癜风患者、18例银屑病患者及19名正常人外周血中CD4+CD25+Treg的表达水平.结果: 白癜风患者的CD4+CD25+Treg表达率为(1.056±0.662)%,低于正常对照组(2.022±0.98)%,差异有统计学意义(P＜0.05);银屑病患者的CD4+CD25+Treg为(1.761±1.396)%,与正常人无显著性差异(P＝0.177).结论: CD4+CD25+Treg细胞在白癜风患者外周血中数量明显减少.     

CD4~+ CD25~+调节性T细胞与IL-17在梅毒血清固定患者外周血中的表达及意义

目的:观察梅毒血清固定患者外周血中CD4+CD25+调节性T细胞和IL-17的表达及意义。方法:采用三色流式细胞术及免疫酶联吸附法测定18例梅毒血清固定患者和18例对照者外周血中CD4+CD25+调节性T细胞和IL-17的表达水平。结果:梅毒血清固定患者外周血CD4+CD25+调节性T细胞的含量明显高于对照组(t=6.29,P<0.01);IL-17的含量与对照组比较差异无统计学意义(t=-1.68,P=0.102)。结论:CD4+CD25+调节性T细胞表达水平增高造成的免疫抑制可能与梅毒血清固定有关;IL-17的表达水平可能与梅毒血清固定无关。     

斑秃患者外周血CD4~+ CD25~+ Treg细胞计数及TGF-β_1的表达

目的通过检测斑秃患者外周血中CD4+CD25+Treg细胞计数、转化生长因子-β1(TGF-β1)的表达水平,探讨斑秃的发病机制。方法采用流式细胞技术检测50例未治疗的斑秃患者外周血中CD4+CD25+Treg细胞的水平变化,ELISA法检测血清中TGF-β1含量,并进行相关性分析,以30例健康者作为正常对照。结果斑秃患者组外周血CD4+CD25+Treg细胞占T淋巴细胞的比率和血清TGF-β1浓度均低于正常对照组(P均<0.01),且重型患者组显著低于正常对照与局限型患者组(P<0.01),局限型患者组低于正常对照组(P<0.05)。斑秃患者外周血CD4+CD25+Treg细胞水平,血清中TGF-β1含量均与SALT评分呈显著负相关(r=-0.566,-0.471;P均<0.01),而两者呈显著正相关(r=0.584,P<0.01)。结论斑秃患者外周血中CD4+CD25+Treg细胞与血清中TGF-β1呈一定相关性,且两者与斑秃病情的严重程度相关;CD4+CD25+Treg细胞的数量减少和TGF-β1表达降低可能与斑秃的细胞免疫失调有关。     

大败毒胶囊对梅毒血清固定患者外周血Treg/Th17比例及CD4~+ CD25~+ T细胞水平的影响

目的:探讨大败毒胶囊对梅毒血清固定患者外周血Treg/Th17及CD4~+CD25~+T细胞水平的影响。方法:随机双盲法将60例梅毒血清固定患者分为对照组与观察组,每组30例,对照组采取常规苄星青霉素治疗,观察组在对照组基础上加以大败毒胶囊口服,比较两组临床疗效、治疗前后Treg细胞、Th17细胞、Foxp3及CD4~+CD25~+T细胞比例变化情况。结果:观察组随访12个月临床治愈率、总有效率分别为83.33%、93.33%,均显著高于对照组的0.00%、43.33%,差异有统计学意义(P0.0001)。观察组末次随访时Treg细胞、Th17细胞、Foxp3、CD4~+CD25~+T细胞比例与对照组比较差异有统计学意义(P0.05)。结论:大败毒胶囊治疗梅毒血清固定疗效明确,能显著纠正外周血Treg细胞/Th17细胞失衡现象,降低CD4~+CD25~+T细胞比例,临床值得推广。     

梅毒血清固定患者外周血CD4+CD25+调节性T细胞比例及功能的研究

目的:通过检测梅毒血清固定患者外周血CD4+CD25+调节性T细胞(regulatory T cells,Treg)比例和功能的改变,探讨Treg在梅毒血清固定现象形成中的作用.方法:收集梅毒血清固定患者26例,正常对照23例,利用流式细胞术分别检测外周血Treg比例及Treg内Foxp3、细胞毒性T淋巴细胞抗原(CTLA)-4及白介素(IL)-10的定量表达情况.结果:梅毒血清固定组患者外周血Treg比例明显高于正常对照组(P＜ 0.01);且Treg内转录因子Foxp3及功能性分子CTLA-4和IL-10的表达量也明显高于正常对照组(P＜ 0.05或P＜0.01).结论:梅毒血清固定患者外周血Treg比例和功能的异常,可能是导致该现象形成的重要原因之一.     

外周血及蜕膜组织中CD4~+ CD25~+调节性T细胞比例和炎症因子原因不明复发性流产的关系研究

目的:探究外周血及蜕膜组织中CD4~+CD25~+调节性T细胞比例以及相关炎症因子对原因不明复发性流产的影响。方法:选取2014年5月至2015年5月就诊于我院门诊的原因不明复发性流产(URSA)患者90例为观察组,并选取同期在妇产科门诊手术室的行人工流产手术的正常患者90例为对照组;分析两组患者的一般资料、外周血及蜕膜组织中CD4~+CD25~+调节性T细胞比例、调节性T细胞特异调节因子Foxp3和EBi3的表达以及血清炎症因子(包括TNF-α、IL-2、IL-6、IL-10)水平,研究其相关性。结果:与正常妊娠组相比,反复流产组外周血及蜕膜组织中Treg细胞比例明显降低,Foxp3和EBi3因子mRNA的表达水平明显降低,且无论URSA患者或者是正常对照组,蜕膜中各因子的表达均高于外周血中的表达,外周血TNF-α、IL-2水平明显升高,而IL-6和IL-10水平要明显降低,差异有统计学意义(P0.05)。结论:蜕膜中各因子的表达均高于外周血中的表达提示妊娠过程的主要免疫反应部位是"母-胎界面",Treg细胞比例、Treg细胞因子和Th2型因子IL-6、IL-10水平可能参与妊娠的维持,调控"母-胎界面"局部免疫耐受。对于复发性流产的发病过程具有一定的影响作用。     

Imbalance of CD4+CD29+ and CD4+CD45RA+ T-helper cell subsets in peripheral blood of patients with severe atopic dermatitis

To determine the proportion of T-helper cell subsets in the peripheral blood we studied 16 patients with mild, moderate and severe atopic dermatitis. Lymphocytes were isolated from heparinized peripheral blood and analysed by two-colour flow cytometry. Patients with severe atopic dermatitis had a decreased CD4+CD29+CD4+CD45RA+ ratio (p<0.01). We found a decreased absolute number of CD4+CD29+ cells (p<0.05) and an increased absolute number of CD4+CD45RA+ cells (p<0.05) in the peripheral blood. No significant changes in the CD4+CD29+CD4+CD45RA+ ratio were found in the peripheral blood of patients with clinically mild or moderate atopic dermatitis.     

脓毒症患者CD14+CD16+单核细胞的作用探讨

目的观察脓毒症患者外周血单核细胞CD14+CD16-、CD14+CD16+、CD14-CD16+三个亚群表达HLA-DR、CD14、CD16的异同,并分析CD14+CD16+单核细胞的特点。方法选取67例脓毒症患者为脓毒症组和15例健康志愿者作为对照组,脓毒症组根据疾病严重程度分为脓毒症（15例）、重症脓毒症（41例）和脓毒性休克组（11例）,收集临床资料,计算APACHE II评分,流式细胞仪检测单核细胞各亚群CD14、CD16、HLA-DR的表达。结果脓毒症患者CD14+CD16+单核细胞数目较正常对照组显著升高（P＜0.01）;脓毒症患者单核细胞HLA-DR、CD14平均荧光强度均较健康对照组明显降低,并随着脓毒症患者病情由轻到重而逐渐下降;各组中CD14+CD16+单核细胞相对高表达HLA-DR、CD14;单核细胞HLA-DR表达与临床指标间存在较好的相关关系。结论①脓毒症患者单核细胞HLA-DR表达显著降低;②CD14+CD16+单核细胞在脓毒症发病的免疫机制中可能起到重要作用。     

CD80CD86 deficiency disrupts regulatory CD4+FoxP3+T cell homoeostasis and induces autoimmune‐like alopecia

Alopecia areata (AA) is an autoimmune disease that results in spot baldness in humans. Adequate animal models for AA are currently lacking. The objective of this study was to elucidate the mechanism of autoimmune-like alopecia (ALA) in C57BL/6.CD80CD86-deficient (B6.CD80CD86^−/−) mice. Incidence and severity of alopecia were analysed in 58 B6.CD80CD86^−/− mice using histological examination, flow cytometry, multiplex enzyme-linked immunosorbent assay, quantitative RT-PCR and CD25 inhibition test. Both male and female B6.CD80CD86^−/− mice showed almost 100% incidence of hair loss at 40 weeks of age. Moreover, CD4+FoxP3+Treg (Treg) cell population in B6.CD80CD86^−/− mice was significantly lower than in B6 mice, which presumably underlined autoimmune reaction. Histologically, B6.CD80CD86^−/− mice showed CD4+ and CD8+ T-cell infiltration around terminal follicle region and exhibited hair follicle destruction in the anagen or catagen stage. Negative correlation between the number of CD4+FoxP3+ Tregs and ALA was confirmed by the CD25 depletion test in B6 mice, as follicle destruction was similar to that observed in B6.CD80CD86^−/− animals. CD80CD86 deficiency disrupted CD4+FoxP3+ Treg homoeostasis and prompted the development of ALA. We demonstrated that B6.CD80CD86^−/− mice might have several advantages as an ALA model, because they exhibited high incidence of disease phenotype and epipathogenesis similar to that observed in human AA.     

白塞病患者外周血CD4+CD25+调节性T细胞的检测及其临床意义

目的检测白塞病(BD)患者外周血Cd4+ CD25+调节性T细胞数量变化并分析其与疾病活动性的关系,探讨其临床意义.方法利用流式细胞仪和单克隆荧光抗体技术测定31例BD患者和27例健康对照者外周血中CD4+CD25+调节性T细胞在CD3+Cd4+细胞中的比例.结果活动期患者外周血中CD4+CD25+调节性T细胞在CD3+CD4+细胞中的百分率(6.43%±2.54%),低于健康对照者(8.17%±2.87%)(P＜0.05).非活动期患者外周血中CD4+CD25+调节性T细胞在CD3+ CD4+细胞中的百分率(8.35%±2.31%),略高于健康对照者(P＞0.05).结论BD患者外周血中CD4+CD25+调节性T细胞在CD3+CD4+细胞中的比例降低与疾病活动相关.通过检测外周血CD4+CD25+调节性T细胞在CD3+ CD4+细胞中的比例来评估机体免疫状态是一个可行的办法.     

Regulation of nickel-induced T-cell responsiveness by CD4+CD25+ cells in contact allergic patients and healthy individuals

In this study, we investigated the capacity of CD4⁺CD25⁺ regulatory T cells to suppress nickel‐specific effector T cells, both in nickel‐allergic patients and healthy controls. CD4⁺ cells isolated from allergic patients showed an increased proliferative response to nickel, whereas CD4⁺ cells from negative controls did not respond to allergen. When CD4⁺CD25⁺ cells were depleted, nickel‐specific responsiveness was strongly increased both in allergic and in non‐allergic individuals, with the most pronounced effect in allergic patients. These regulatory T cells were anergic to nickel but inhibited nickel‐specific CD4⁺CD25^– effector T cells in coculture experiments. CD4⁺CD25⁺ cells from nickel‐allergic patients showed only a limited capacity to suppress effector T‐cell responsiveness, because an increased nickel reactivity could still be detected in these cocultures. None of the isolated CD4⁺CD25⁺ cells, either isolated from healthy controls or allergic patients, produced IL‐10 in response to nickel. Overall, these results support the view that CD4⁺CD25⁺ cells can control the activation of nickel‐specific effector T cells in non‐allergic individuals, whereas this regulatory capacity is impaired in allergic patients. To investigate the presence of allergen‐specific regulatory T cells in truly naïve, non‐sensitized individuals, T‐cell reactivity should also be studied with non‐environmental contact allergens, such as para‐phenylenediamine.     

CD8~+的淋巴瘤样丘疹病1例

患者女,38岁。全身反复红斑、斑片8年,丘疹、结节1年。全身多处泛发色素沉着斑及红色小丘疹,左腰部有一直径1cm大小的斑块。斑块处组织病理示:真皮及皮下弥漫大细胞,有异形性;免疫组化:异形细胞LCA(+)、CD3(+)、CD4(+)、CD8(+)、CD30(+)、GranzymeB(+),诊断为淋巴瘤样丘疹病,A型。该患者以红斑起病之后出现丘疹,但从未出现溃疡坏死,丘疹的大小和可自行消退的特点符合淋巴瘤样丘疹病,CD8+是LyP较为少见的免疫表型。该病应与皮肤CD30(+)间变性大细胞淋巴瘤和蕈样肉芽肿鉴别,需监测其预后及转归。     

Evaluation of tumour-infiltrating CD4+CD25+FOXP3+ regulatory T cells in human cutaneous benign and atypical naevi, melanomas and melanoma metastases

BACKGROUND: CD4+CD25+FOXP3+ regulatory T cells (Tregs) are thought to induce immunotolerance in melanoma. They have not yet been investigated in the entire spectrum of melanocytic cutaneous lesions within a tumour site. OBJECTIVES: To evaluate CD4+CD25+FOXP3+ Tregs among tumour-infiltrating lymphocytes in cutaneous melanocytic lesions. METHODS: We analysed 128 lesions (10 benign junctional common naevi, 10 benign compound common naevi, 10 compound Spitz naevi, 10 junctional atypical naevi, 20 compound atypical naevi, 20 radial growth phase melanomas, 30 vertical growth phase melanomas and 18 melanoma metastases). Tregs were identified by CD25-FOXP3 double immunostains. RESULTS: This study indicates that CD4+/CD25+FOXP3+ Tregs are present in all groups of lesions. Junctional atypical naevi, compound atypical naevi and radial growth phase melanomas showed the highest percentages of CD4+CD25+FOXP3+ Tregs (junctional atypical naevi vs. junctional common naevi, compound common naevi, compound Spitz naevi, melanoma metastases: P < 0.0001; junctional atypical naevi vs. vertical growth phase melanomas: P = 0.001; compound atypical naevi vs. junctional common naevi, compound common naevi: P < 0.0001; compound atypical naevi vs. compound Spitz naevi, melanoma metastases: P = 0.002; compound atypical naevi vs. vertical growth phase melanomas: P = 0.02; radial growth phase melanomas vs. junctional common naevi, compound common naevi, compound Spitz naevi, melanoma metastases: P < 0.0001; radial growth phase melanomas vs. vertical growth phase melanomas: P = 0.008). CONCLUSIONS: The strong prevalence of CD25+FOXP3+ Tregs both in junctional and compound atypical naevi and radial growth phase melanomas, suggests that they induce immunotolerance early during melanoma genesis, favouring melanoma growth. Their evaluation within a tumour site could be useful for prognostic and therapeutic purposes.     

免疫表型为CD8~+,CD4~-,CD56~+的蕈样肉芽肿1例

报告1例免疫表型为CD4-,CD8+,CD56+的蕈样肉芽肿(MF)。患者男,21岁。双侧腋窝、躯干及腹股沟大片状灰红色至棕红色斑8年,呈渐进性发展,无任何自觉症状。腹部皮损组织病理示表皮轻度角化不全,真皮内致密淋巴细胞,部分细胞入侵表皮。免疫组化示CD4-,CD8+,CD3+,CD43+,CD68灶状+,CD30灶状+,CD56+,TIA-1-,EBV-。诊断:蕈样肉芽肿。给予阿维A胶囊30mg/d,顿服,糠酸莫米松乳膏及多磺酸粘多糖乳膏外用。皮损缓解,临床随访4个月,病情稳定。     

慢性荨麻疹患者外周血中CD8~+CD28~-T细胞检测结果分析

目的:探讨CD8~+CD28~-T细胞在慢性荨麻疹患者致病机制中的作用。方法:收集临床慢性荨麻疹(CU)病例83例,同时设立对照组64例进行比较。应用ELISA法检测研究对象血清中抗Ig E抗体、抗FcεRⅠ抗体浓度,流式细胞仪检测外周血中CD3~+、CD4~+、CD8~+、CD8~+CD28~-、CD4~+CD25~+T细胞比例,分析检测结果。结果:83名CU中,23例抗Ig E抗体为阳性,占27.7%(27/83);31例抗FcεRⅠ抗体为阳性,占37.3%(31/83)。CU患者外周血CD8~+T细胞比例、CD8~+CD28~-T细胞比例低于正常对照组(P0.05),CD4~+/CD8~+比值、CD4~+CD25~+T细胞比例均高于对照组(P0.05)。结论:CU患者机体外周血CD8~+CD28~-T细胞、CD4~+CD25~+T细胞比例与对照组相比存在差异,CD8~+CD28~-T细胞比例降低也可能是CU致病机制之一。