乙酰胆碱对人表皮细胞冷存的影响

目的：探讨乙酰胆碱（Ach）对人表皮细胞冷存活力的影响。方法：应用人表皮细胞的分离、培养技术，并进行MTT检测、病理切片以及电镜检测等手段进行观察。结果：不同浓度的Ach对冷存的表皮细胞均有一定的保存活力作用，其中以10^-1-10^-10mol/L的浓度为最佳。结论：认为Ach对冷存中的人表皮细胞有一定的保存作用，它可提高表皮细胞的保存活力。     

六亚早基二乙酰胺体外诱导人粘液表皮样癌细胞分化的实验研究

用六亚甲基二乙酞胺（ＨＭＢＡ）作为分化诱导剂，对人涎腺粘液表皮样癌细胞系ＭＥＣ－１细胞进行体外诱导（诱导浓度１ｍｍｏｌ／Ｌ），探讨ＨＭＢＡ对粘液表皮样癌细胞的诱导分化作用。实验经细胞生长动力学、细胞ＤＮＡ含量、核仁区嗜银蛋白染色指标观察，显示在ＨＭＢＡ作用下ＭＥＣ－１细胞有向成熟细胞分化的趋势，而且诱导所需浓度低，分化作用明显，有进一步研究价值。     

表皮细胞培养最佳条件的探索

目的：研究表皮细胞培养的最佳条件,为进一步将其作为皮肤组织工程种子细胞奠定基础。方法：以DMEM作为基础培养液,改变培养条件后,计数2周内克隆形成数,检测细胞增殖能力,确定最佳条件。结果：表皮细胞以分离酶分离可得到大多数基底细胞,在培养液pH值为7．0－7．2,钙离子浓度为0．4mmol/L,血清浓度为18％,温度为36℃,CO2浓度为6％时,表皮细胞克隆形成数量高。结论：在最佳培养条件下,表皮细胞生长状态良好,为人工表皮的构建奠定了基础。     

丝素蛋白对表皮细胞生长的影响

目的：了解丝素的生物学性能和丝素对表皮细胞生长的影响。方法：试验组采用培养液ＤＭＥＭ 加５％ 丝素蛋白原液,对照组采用培养液ＤＭＥＭ 加５％ 生理盐水的方法,对表皮细胞培养结果进行比较观察;观察指标为表皮细胞形态、细胞生长曲线、３Ｈ－ 胸腺嘧啶核苷测定等。结果：培养后３ 天试验组大量细胞贴壁生长,而对照组在培养后５ 天才大量贴壁生长;试验组细胞数在５ 天明显增多,对照组在７ 天才迅速增加;３Ｈ－ 胸腺嘧啶核苷测定试验组及对照组的增殖活力均在第７ 天达高峰,但同一时相点试验组增殖活力明显高于对照组。结论：丝素蛋白对表皮细胞生长具有促进作用。     

移植培养表皮细胞片时创面局部抗生素的合理选用

目的：优选出适于移植培养表皮细胞片（ＣＥＡ）的局部抗菌用药方案。方法：将培养的人表皮细胞置于含不同浓度抗生素的培养液中培养３天后，用噻唑蓝（ＭＴＴ）法测定其细胞生长率，同时采用定量铂环法和Ｋ－Ｂ纸片扩散法分别测定其在创面上和在体外的抗菌效果；并以此分别准备烧伤或肉芽创面，观察了对ＣＥＡ早期成活的影响。结果：对培养的人表皮细胞毒性作用为：碘伏（ＰＶＰ－Ⅰ）＞磺胺嘧啶银（ＡｇＳＤ）氟哌酸银（ＡｇＮ）＞丁胺卡那霉素（ＡＫ）／ＡＫ与ＰＲ复合抗生素（ＤＡＢ）＞氧哌嗪青霉素（ＰＲ）；ＡｇＮ和ＤＡＢ在临床使用浓度时抗菌效果较好，以此分别准备烧伤或肉芽创面，显著地提高了ＣＥＡ早期成活率。结论：烧伤创面外用１％ＡｇＮ、肉芽创面外用０．１％ＡＫ和０．２％ＰＲ的ＤＡＢ液湿敷、术后局部预防性应用０．０１％ＡＫ和０．０２％ＰＲ的ＤＡＢ液，可有效地提高ＣＥＡ的早期成活率。     

B16细胞中FLT3表达的检测及FLT3—Ligand对B16细胞生长的？…

目的;检测造血生长因子受体ＦＬＴ３在非造血来源的黑素瘤细胞系Ｂ１６中的表达,并研究其配基ＦＬＴ３－Ｌｉｇａｎｄ（ＦＬ）对ＧＬ１６细胞的作用。方法：通过ＲＴ－ＰＣＲ检测Ｂ１６细胞中ＦＬＴ３ｍＲＮＡ的表达,流式细胞仪检测ＬＦＴ３蛋白在Ｂ１６细胞表面的表达,在培养液中加入不同浓度的ＦＬＴ对Ｂ１６细胞进行培养。结果：ＦＬＴ３在Ｂ１６细胞中有ｍＲＮＡ的表达,在Ｂ１６细胞表面有蛋白质的表达。     

灵芝多糖对小鼠T细胞IL—2IL—3mRNA表达的影响

目的：研究灵芝多糖GLB7对小鼠T细胞IL－2，IL－3mRNA表达的影响，进一步探讨灵芝多糖免疫调节作用的机制。方法：培养开始时加入不同浓度的GLB7（5，10，20，40mg.L^-1)，培养一定时间后，以RT－PCR和凝胶图像吸收度分析系统检测灵芝多糖（GLB7）与小鼠脾脏T细胞IL－2，IL－3 mRNA表达之间的量效关系。结果：GLB7能剂量依赖性增强静息IL－2和IL－3 mRNA的表达，分别于11h和20h达到高峰，而对活化的T细胞则不产生影响，此时培养上清中IL－2和IL－2活性与对照组比较亦有明显升高，并具有一定的剂量依赖关系。结论：灵芝多糖免疫增强和抗肿瘤作用的基础与其在转录水平促进T细胞中IL－2和IL－3 mRNA的表达有关。     

表达hEGF导入基因的角朊细胞促进体外培养的正常角朊细胞增殖

目的 观察含外源性人表皮细胞生长因子（ｈＥＧＦ）基因改造的角朊细胞（ｈＥＧＦ－ＭＨＫ），对正常培养的角朊细胞（ＮＨＫ）增殖的影响；探讨转ｈＦＧＦ对创面愈合的生物学效应及作用机理。方法 收集含真核表达质粒ｐｃＤＮＡ３－ｈＥＧＦ的Ｈｅｇｆ－ＭＨＫ细胞培养上清，采用放射免疫分析法检测收集的上清液中ｈＥＧＦ含量；以１０％的浓度加入由正常人皮肤组织分离培养的ＮＨＫ细胞培养基内；ＭＴＴ法测定ＮＨＫ细胞增减１５     

含表皮细胞的无细胞真皮复合皮的构建及生长活性研究

为探讨体外构建复合皮的可能性，制备来源于猪皮的无细胞真皮基质，在其表皮面种植表皮细胞于体外培养，定期消化分离无细菌真皮上的表皮细胞，采用细胞计数法和增殖试验观察表皮细胞增殖活力，并分别于1周和2周后通过组织切片HE染色、扫描电镜观察细胞生长状况。结果显示，表皮细胞随培养时间延长，数量明显增多，且仍具有一定的增殖潜力。培养1周、2周后分别形成单层细胞膜片及含3－6层细胞的复层膜片。提示以异种无细菌真皮为载体，可于体外成功构建含表皮细胞层的活性复合皮。     

葛根素对火药烟雾诱导支气管上皮细胞凋亡的防护作用

目的 探讨葛根素对火药烟雾诱导的支气管上皮细胞(BEAS-2B)凋亡的保护作用及其机制.方法 体外培养BEAS-2B细胞,随机分为对照组(不加任何干预)、烟熏组(火药烟雾4g,作用10min)、烟熏+葛根素组(12.5、25、50、100μg/ml).细胞接种12h后加入葛根素,继续培养至24h后烟雾作用10min,继续培养2h后进行检测.采用CCK-8试剂盒检测细胞活力,Hoechst染色观察细胞凋亡情况,流式细胞仪检测Annexin V-PI双染细胞及caspase-3阳性率.结果 与对照组相比,烟熏组BEAS-2B细胞活力明显下降(P＜0.01),不同浓度葛根素能够不同程度地对抗烟熏的作用,其最佳保护浓度为25μg/ml.与烟熏组相比,烟熏+葛根素组Hoechst染色细胞凋亡率、Annexin V-PI双染细胞凋亡率及caspase-3阳性率均明显降低(P＜0.05,P＜0.01).结论 火药烟雾可致体外BEAS-2B细胞凋亡,而葛根素对火药烟雾诱导的BEAS-2B气管上皮细胞凋亡具有一定防护作用.     

Optimization of the system of medical service of students of Cadet Schools of the Ministry of Defense of the Russian Federation

One of the factors of the successful military career guidance Cadet schools students is preserving and promoting their health. Medical support of children and adolescents aged 10-17 years should include the full range of medical and preventive measures defined for this group. The state of providing outpatient care for pupils at the Cadet School in St. Petersburg was studied. These results show that full medical care in accordance with the standards can be based only on children's health clinics. It is important that the organization of medical support pupils cadet schools should be cooperate with civilian health care.     

带状疱疹83例误诊原因分析

带状疱疹是由水痘—带状疱疾病毒引起的皮肤科常见疾病。其主要的病理损害,一是受累神经的严重炎症性浸润,继而导致受侵犯神经节内神经细胞变性、坏死;二是皮肤的水泡。迅速抑制神经节和相应的感觉神经纤维的充血、水肿和坏死,防止粘连形成,达到迅速镇痛、改善皮损,缩短病程及防止后遗症的发生是治疗的关键。因而,尽早明确诊断,     

Analysis of the results of the international comparison of activity measurements of a solution of Fe

The results of an international comparison of activity measurements of a solution of ⁵⁵Fe organized by the BIPM in 2005 are reported and analysed. This exercise, which follows the procedures of the CIPM mutual recognition arrangement to update older comparisons, is a renewal of the comparison organized by the BIPM that took place in 1978. A EUROMET comparison was organized in 1996 specifically to compare activity measurements of a ⁵⁵Fe solution by means of liquid-scintillation techniques. Results of these three comparisons are presented and discussed in this paper.

The radionuclide solution was provided by the NPL, which also distributed the samples to the participants. The activity of the ampoules was measured by 16 laboratories using 12 methods producing 25 results. Some general considerations on uncertainty assessments pertaining to the different techniques used are drawn. The outcome of four different estimators is compared from which the presence of at least one outlier can be confirmed. Further measurements should be made to try to reduce the discrepancy between the results. To date the outcome of the present comparison does not show an improvement to that of the 1996 comparison.     

Estimation of accumulated dose of radiation by method of ESR-spectrometry of dental enamel of mammals

ESR-spectrometry was used to investigate radiation-induced paramagnetic centers in enamel of mammals: carnivores (polar bear and fox), ungulates (reindeer, European bison, moose), and man. Values at half the microwave power saturation of the radiation signal, P_1/2, evaluated at room temperature, was found to range from 16 to 26 mW for animals and man. A new approach to discrimination of the radiation induced signal from the total ESR spectrum of reindeer enamel is proposed. ‘Dose-response’ dependencies of enamel of different species mammals were measured within the dose range from 0.48 up to 10.08 Gy. Estimations of ‘radiosensitivity’ enamel of carnivores and ungulates showed good agreement with radiosensitivity enamel of man by ESR method.     

Evaluation of the results of various technics of non-surgical therapy of varicocele

A new method of non-surgical treatment of varicocele syndrome is described: it consists in sclerotherapy of spermatic vein by trans-femoral percutaneous catheterization with balloon-catheters. In 8 cases venous thrombosis has been induced by direct electric clotting. The techniques and a 6 months follow-up are discussed. It is pointed out that this procedure should be considered as the method of choice for tubular lesions and sub-fertility prophylaxis in young people and in childhood.     

延迟性脾破裂误漏诊原因分析

目的探讨延迟性脾破裂误漏诊原因和预防措施.方法回顾性分析总结12例延迟性脾破裂中的诊断和误漏诊的经验与教训.结果本组延迟性脾破裂的误漏诊5例(41.66%).对多发伤与脾破裂并存可能认识不足,外伤史轻微或伤员隐瞒外伤史,缺乏腹痛-缓解-突然再腹痛的典型病史,缺乏“对冲性脾破裂”力学分析和整体化诊断思路等为其误漏诊的主要原因.结论详细的外伤史和全面系统检查,重视腹以外多发伤掩盖腹内脏器伤及延迟性脾破裂可能.确立外伤-腹内脏器伤-脾破裂整体化诊断思路.不间断地辅以B超检查脾形态学变化和腹内有无积液,腹腔穿刺确定有无血腹、X线胸腹部检查观察左侧胸肋角和膈肌运动情况、必要时CT检查以尽早发现脾包膜下血肿,降低延迟性脾破裂误漏诊率.