血清胸腺因子的研究进展

概述了血清胸腺因子(STF)的分离纯化、结构、特性、测定,体内分布、生物学作用及其与某些疾病的关系和潜在的药用价值。     

血清胸腺因子（FTS）的抗肿瘤作用

目的:观察血清胸腺因子(FTS)对人肝癌细胞的抑制作用。方法:裸鼠皮下接种人肝癌细胞 Hepg-2,待肿瘤生长后连续皮下注射不同剂量的 FTS,测定其抑瘤率;并测定 T 细胞增殖能力、吞噬指数(PI)等免疫指标。结果:荷瘤裸鼠皮下注射高、中剂量后抑瘤率均大于30%,且与对照组(NS)有显著性差异(p<0.01),低剂量组与 NS 组无显著性差异。另外 ConA 刺激后0.016 mg·mL~(-1)处刺激指数(SI)最大,FTS 组 PI 明显大于 NS 组。结论:FIS 对人肝癌细胞 Hepg-2有抑瘤作用且增强其免疫功能。     

血清胸腺因子抗EAC瘤、H22瘤、W256瘤作用的初探

目的：观察血清胸腺因子（FTS）对荷瘤小鼠的抑瘤作用。方法：荷EAC瘤小鼠、荷H22瘤小鼠、荷W256瘤大鼠分别皮下注射给予不同剂量的FTS,测定其生命延长率和抑瘤率,并测定T细胞增殖能力、迟发型超敏反应等免疫指标。结果：荷EAC、H22瘤小鼠注射FTS0.25、0.13mg/kg剂量后生命延长率或抑瘤率均大于30%,生存天数和瘤重与阴性对照组（NS）比较有统计学差异（P〈0.01）;荷W256瘤大鼠FTS各组瘤重与NS组比较均有统计学差异（P〈0.01）,抑瘤率均大于30%。另外,伴刀豆球蛋白A（ConA）刺激后0.016mg/mL处刺激指数最大。迟发型超敏反应亦呈现阳性结果。结论：FTS对EAC瘤、H22瘤、W256瘤均具有一定的抑制作用。     

胸腺因子D对小鼠寿命影响的实验研究

目的 　观察胸腺因子 D(TFD)对小鼠寿命的影响。 方法 　采用昆明种配对鼠 ,雄性 14对 ,雌性 11对。分组后分别饲以标准颗料饲料 ,2月以后用药组使用掺以含 TFD5 g·kg- 1的颗粒饲料 ,每天记录小鼠死亡数。 结果 　 5 0 %小鼠死亡时间和 85 %小鼠死亡时间的平均寿命 ,TFD组比相同性别对照组长 ,差异显著 (P<0 .0 5 )。 结论 　提示 TFD具有较好的抗衰老作用     

胸腺因子D对复发性口疮的免疫调节作用

采用双育法将１０６例ＲＡＮ患者随机分为Ａ、Ｂ两组。分别用ＴＦＤ２ｍＬ（５ｍｇ）或核酷２ｍＬ,隔日１次,连续肌注６０ｄ。在治疗前后测定细胞免疫和体液免疫指标。结果与正常对照组比较ＲＡＵ患者外周血淋巴细胞数减少,ＥＲＦＣ、ＣＤ４＋、ＣＤ８＋值降低;ＩｇＧ、ＩｇＡ、ＩｇＭ、Ｃ３水平升高。ＴＦＤ可使淋巴细胞数、ＥＲＦＣ、ＡＲＦＣ、ＣＤ３＋、ＣＤ４＋、ＣＤ８＋值升高;ＩｇＧ、ＩｇＡ、ＩｇＭ、Ｃ３值复常。     

胸腺因子D的抗应激作用与智能实验

本文采用昆明种鼠探讨胸腺因子Ｄ的抗应和用及其对智能的影响。     

国外自身免疫调节因子在免疫系统发展中作用的研究

自身免疫调节因子是一个为相当大部分遗传类型自身免疫性疾病负责的基因.自身免疫调节因子缺陷导致自体免疫疾病的发病机制是现在研究的关键,它可以为胸腺微环境内免疫系统区别自我或非我提供线索.     

胸腺因子D和胸腺酶对胃癌MGC803细胞株的抑制作用

单个癌细胞及其微环境与宿主整体之间的关系,对于治疗是至关重要的。生物反应调节剂胸腺因子Ｄ（ＴＦＤ）及其和超氧化物歧化酶的复合制剂胸腺酶（ＴＦＳ）,对胃癌ＭＧＣ８０３细胞生长的抑制试验表明,高浓度（４．０￣０．５ｍｇ／ｍｌ）的抑制率均在５０％以上,低浓度（０．２５￣０．０３１５ｍｇ／ｍｌ）的抑制率在４８％￣１０％之间。人工胃液和人工肠液加入制剂中作用０．５￣２ｈ,对其抑制率无明显影响,ＴＦＤ和ＴＦＳ     

扇贝多肽对UVB辐射小鼠胸腺淋巴细胞的保护作用

目的：研究扇贝多肽(polypeptide ftom Chlamys，farreri)对中波紫外线(UVB)辐射损伤小鼠胸腺淋巴细胞的保护作用。方法：UVB辐射小鼠胸腺淋巴细胞，MTT法检测胸腺淋巴细胞的活性；酶生化法检测细胞谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性；流式细胞术检测细胞内活性氧(ROS)的产生量以及细胞凋亡率。结果：PCF能明显减轻UVB辐射对小鼠胸腺淋巴细胞的损伤；提高细胞GSH-Px、SOD和CAT活性；降低细胞中ROS的产生量和细胞凋亡率。结论：PCF对小鼠胸腺淋巴细胞具有明显的辐射保护作用。     

竹节参多糖对高血脂模型小鼠的影响作用

目的:研究竹节参多糖(D1)降血脂的药理活性。方法:腹腔注射75%蛋黄生理盐水溶液0.02mL.g-1建立小鼠高血脂动物模型。随机分为空白对照组、高脂模型组、2个阳性对照组(血脂康组及辛伐他汀组)和2个试验组(D1高剂量组及D1低剂量组)。空白对照组给予生理盐水,高脂模型组给予蛋黄乳溶液和生理盐水,阳性对照组和试验组以羧甲基纤维素钠混悬,配成混悬药液样品。测定给药后动物血浆中TG、TC、LDL-C、HDL-C的浓度。结果:阳性对照组和试验组各项指标与模型组比较,差异均有显著性。结论:竹节参多糖具有一定的降血脂的作用,并存在量效关系。     

牛黄天龙方药对荷瘤小鼠免疫功能的影响及其抗肿瘤作用的实验研究

目的了解中药复方牛黄天龙方药对荷瘤小鼠免疫功能方面的影响。方法建立H22荷瘤小鼠模型;分高、中、低3个剂量组灌胃给药,疗程结束后,取小鼠血清,检测白细胞介素-2（IL-2）及肿瘤坏死因子（TNF—α）水平;分离脾细胞培养,测定自然杀伤细胞（NK）,淋巴因子活化杀伤细胞（LAK）活性;分离睥细胞检测T淋巴细胞亚群。结果牛黄天龙方药具有明显增强H22荷瘤小鼠细胞免疫功能的作用并可延长荷瘤小鼠的生存时间（P〈0．05）。结论牛黄天龙方药对H22荷瘤小鼠具有显著免疫调节的抗肿瘤作用。     

雌激素改善小鼠学习记忆的实验研究

目的 :研究雌激素去除 (OVX)与雌激素替代 (ERT)对小鼠T迷宫学习记忆的影响。方法 :3mo鼠龄的雄性C 5 7BL/ 6J小鼠 6 0只 ,随机分 2组 ,分别为假手术组和卵巢切除组 ,每组又分为不给雌激素、给雌激素 7d、给雌激素 4 0d的 3个亚组 ,每组 10只。小鼠摘除卵巢后饲养至 7mo鼠龄进行迷宫实验 ,将 2组小鼠于迷宫测试前 7d和 4 0d分别埋植控释雌激素 (17β 雌二醇 )药丸每丸 0 .18mg ,测试各组小鼠对T迷宫的正确选择次数。结果 :卵巢切除组不给雌激素亚组其正确选择次数明显下降 ,与假手术组的不给雌激素亚组比较 ,d 1,d2和d 3为 (42± 10 ) %vs (5 4± 9) % ,(47± 11) %vs (6 2± 10 ) %和 (5 0± 10 ) %vs (6 8± 10 ) %均P<0 .0 1;给雌激素 7d和 4 0d亚组其正确选择次数明显提高d 1,d 2和d 3为 (5 7± 7) %vs (42±10 ) % ,(6 5± 11) %vs (47± 11) %和 (77± 11) %vs(5 0± 10 ) % (P <0 .0 1) ,而且长期给雌激素亚组其正确选择次数明显高于短期给雌激素亚组。假手术组的不给雌激素和给雌激素组其正确次数差异无显著意义 (P >0 .0 5 )。结论 :雌激素去除明显损害了小鼠的学习记忆能力 ,而雌激素替代可以改善小鼠的学习记忆能力 ,正常小鼠予雌激素负荷并不能影响其学习记忆过程     

针刺联合药物对小鼠肿瘤细胞抑制性作用的实验研究

目的 观察针刺与氟尿嘧啶、益气解毒中药复方联合应用对荷瘤小鼠肿瘤细胞的抑制性作用.方法 制备小鼠肝癌H22细胞实体瘤模型,分成肝癌模型组、化疗药物组（氟尿嘧啶腹腔注射）、益气解毒方组（中药灌胃）、针刺大椎穴组、化疗药物＋针刺大椎穴组、益气解毒方＋针刺大椎穴组,并与正常小鼠进行对照.观察荷瘤小鼠一般生存状态,并计算各组肿瘤抑制率.结果 化疗药物组小鼠一般状态较差,益气解毒方组及针刺组一般状态均优于化疗药物组,针药联用组小鼠的一般生存状态优于模型组与单一疗法组;各实验组对肿瘤均有明显抑制作用（P＜0.01）.结论 针刺联合药物对小鼠肿瘤细胞的生长具有较好的抑制作用.     

孕鼠铅暴露对仔鼠血铅水平影响的研究

目的探讨孕鼠铅暴露对仔鼠血铅水平的影响。方法wistar大鼠42只,雌雄各半,按雌雄配对分为五组（A、B、C、D、E）。A组为正常对照组,进食正常饲料B、C、D、E组每公斤饲料分别添加醋酸铅0．3g,0．9g,2．7g,8．1g,产仔后恢复正常饲料。母鼠与仔鼠组a—e1、2于生后1、3周分别进行血铅测定。结果仔鼠血铅水平与母鼠血铅水平呈正相关（r=0．517）。结论胎鼠血铅水平与母鼠孕期铅暴露的程度相关。     

Effects of acute or chronic polychlorinated biphenyl ingestion on maternal metabolic homeostasis and on the manifestations of embryotoxicity caused by cyclophosphamide in mice

Diets of mice contained Aroclor 1254 (PCBs; 0, 1, 10 or 100 ppm). Some groups ingested PCBs beginning on gestation day (gd) 6 (vaginal plug positive day = gd 0) and continuing throughout pregnancy (acute exposure). Others were put on PCBs 90 days before mating and continued (chronic exposure) until all animals were sacrificed on gd 18. None of the PCBs diets had adverse effects on offspring. Chronic ingestion of 100 ppm reduced the conception rate. All chronic and acute 100 ppm pregnant mice had significantly altered ratios of liver to body weight. The aim of the different pretreatment regimens was to affect to variable degrees metabolism of cyclophosphamide (CPA). Bioactivation in the dam's liver is a prerequisite for embryotoxic effects in mice. Two doses of CPA were selected that caused malformations in 21.6% (10mg/kg) or 93.4% (16 mg/kg) of the fetuses when injected on gd 11. The interaction (enhancement, no effect, attenuation) between acute or chronic PCBs and CPA could thus be examined. Double stained fetal limbs and their bone lengths were evaluated. The observations were correlated with cytochrome P-450, the levels of which were elevated in all 100 ppm groups. The activities of enzymes coupled to this hemoprotein family were induced in maternal liver in an exposure mode and PCBs concentration-dependent manner. Body burdens of PCBs in dams and fetuses were related to diet levels in acutely, but not in chronically treated animals. Placental transfer of radioactivity from ring-labelled ¹⁴C-CPA, measured in control and 100 ppm chronic mice, was not significantly affected by PCBs. Contrary to what one would expect from the P-450 levels and presumably induced monooxygenase catalyzed CPA metabolism, the effects on limb development were attenuated. The amelioration observed in mice differs markedly from the effects recently reported in rats, where induction of CPA metabolism enhanced its teratogenicity.Supported by U.S. Public Health Service grant NIH-ES02461. Presented in part at the 66th annual meeting of the Federation of American Societies for Experimental Biology in April 1982 in New Orleans, Louisiana, at the 22nd annual meeting of the Teratology Society in June 1982 in French Lick, Indiana, and at the International Symposium on PCBs in the Great Lakes in March 1982 at Michigan State University in East Lansing, Michigan.Dedicated to Professor Dr. med. Helmut Kewitz on the ocassion of his 65th birthday     

毛冬青素L7抗小鼠缺氧作用

目的:研究毛冬青素L7抗小鼠缺氧作用。方法:通过常压耐缺氧、亚硝酸钠中毒、氰化钾中毒、利多卡因中毒、夹闭气管小鼠心电消失时间、断头小鼠张口动作持续时间等实验观察其抗小鼠缺氧作用。结果:毛冬青素L7可明显延长小鼠在常压缺氧条件下的存活时间;延长亚硝酸钠、氰化钾、利多卡因中毒后的小鼠存活时间;延长夹闭气管小鼠心电消失时间及断头小鼠的喘气时间。结论:毛冬青素L7对缺氧有保护作用。     

Radiopharmacokinetic and dosimetric parameters of 188Re-lanreotide in athymic mice with induced human cancer tumors

Radiolabeled peptides, like the somatostatin analogs, have been used for peptide receptor-mediated radionuclide therapy (PRMRT) in metastatic neuroendocrine tumors.

The eight amino acid peptide 3-(2-naphthalenyl)-d-alanyl-l-cysteinyl-l-tyrosyl-d-tryptophyl-l-lysyl-l-valyl-l-cysteinyl-l-threoninamide,cyclic(2 → 7)-disulfide (9Cl) (lanreotide) was found to bind to the five somatostatin tumor receptors. Lanreotide has been labeled via the bifunctional chelating agent, DOTA, to ¹¹¹In, and ⁹⁰Y. A direct labeling method was used to label lanreotide with ¹⁸⁸Re. Athymic mice with implanted human cancer tumors (uterine-cervix, renal, and neuroblastoma) were injected with radiochemically pure ¹⁸⁸Re-lanreotide (1.11 MBq). The percent injected activity (%IA/g) from serial blood samples was the input data for the WinNonlin computer program to obtain radiopharmacokinetic parameters. The organs’ percent injected activity per gram of tissue (%IA/g) was extrapolated to the weights of a 70 kg male model organs and the number of nuclear transitions (N) were the input for the OLINDA/EXM program to obtain dosimetry estimates. Induced uterine-cervix tumors (HeLa cells) show a mean 2.4 %IA/g uptake up to 24 h and the tumor/blood ratio was over 1.85 (1.5–24 h post-injection) confirming ¹⁸⁸Re-lanreotide remains bound to the tumor. The estimated tumor absorbed dose was 460 mGy/MBq. Human effective dose was 0.0182 mSv/MBq. Therefore, ¹⁸⁸Re-lanreotide is a good candidate for PRMRT and a clinical trial is being planned in order to acquire individual dosimetric data.     

盐酸苯环壬酯对小鼠的围产期毒性试验研究

盐酸苯环壬酯分成２．５、１２．５和６２．５ｐｐｍ３个浓度组，溶于自一不中，供孕鼠从妊娠ｄ１５开始至断乳时饮用。结果显示，在相当于人临床用量１５－７５倍剂量范围内，该药对小鼠胚胎后期生长发育、母鼠分娩、哺乳及新生小鼠神经行为发育无明显影响。６２．５ｐｐｍ组孕鼠给药期间体重增长抑制，死产仔鼠数增多，雄仔鼠肝脏重量增加，表现出一定的母体毒性和发育毒性。     

甲羟孕酮辅助老年性肺癌化疗生存状况的临床观察

目的观察甲羟孕酮在老年性肺癌化疗期间及化疗后,对食欲、进食状况、体重、胃肠道反应以及全身情况的辅助作用。方法观察50例老年性肺癌化疗加甲羟孕酮辅助治疗病人与50例单纯化疗病人治疗期间及化疗后,食欲、食量、体重变化、胃肠道反应以及全身情况变化的比较。结果治疗组82％食欲有改善,78％食量增加,34％化疗期间无明显胃肠反应,KPS评分增加大于10分占66％。单化组食欲,食量无一例改善,仅4％无恶心、呕吐,KPS评分大于10分占6％,差异有显著性。结论甲羟孕酮辅助老年性肺癌化疗作用明显,有提高食欲,降低化疗胃肠道反应,提高生活质量的作用。