栗鼠圆窗置氯化钠高渗液对耳蜗血流和内耳电生理的影响

目的：研究０－５ ｍｏｌ／Ｌ,１－５ ｍｏｌ／Ｌ和６－１ ｍｏｌ／Ｌ高渗ＮａＣｌ 溶液置栗鼠圆窗对内耳电生理和耳蜗血流的影响。方法：动物全身麻醉,暴露圆窗,分别放置０－５ ｍｏｌ／Ｌ,１－５ ｍｏｌ／Ｌ和６－１ ｍｏｌ／Ｌ的ＮａＣｌ 溶液棉片,观察２ ｈ 内听神经复合动作电位（ＣＡＰ） 阈值及耳蜗血流（ＣＢＦ） 改变情况。结果：０－５ ｍｏｌ／Ｌ,１－５ ｍｏｌ／Ｌ和６－１ ｍｏｌ／Ｌ三种ＮａＣｌ 溶液分别可提高ＣＢＦ７－６ ％ ,２４－５％ 和３５－３％ ,０－５ ｍｏｌ／Ｌ的ＮａＣｌ 溶液对ＣＡＰ阈值提高不明显,１－５ ｍｏｌ／Ｌ和６－１ ｍｏｌ／Ｌ的ＮａＣｌ溶液明显提高,以高频区为显著,二者相比以６－１ ｍｏｌ／ＬＮａＣｌ 溶液引起的听力损伤为重。结论：ＮａＣｌ 高渗溶液棉片置圆窗可增加耳蜗血流量;高渗ＮａＣｌ 溶液对内耳听功能的影响随浓度的增加而增大     

自动超氧化物歧化酶活性分析仪的研究

目的研究和评价自动超氧化物歧化酶活性分析仪。方法通过正交试验分析影响测试的因素并研究最佳测试条件等。结果①影响测试结果的因素依次为：Ｔｒｉｓ－ＨＣＬ缓冲液的ｐＨ值＞焦性没食子酸浓度＞Ｔｒｉｓ－ＨＣＬ缓冲液的体积＞Ｔｒｉｓ－ＨＣＬ缓冲液的浓度＞搅拌速度；②最佳测试条件为：在２５℃时，０．１ｍｏｌ／Ｌ，ｐＨ８．２的Ｔｒｉｓ－ＨＣＬ缓冲液０．２ｍｌ，样品用量５～２０μｌ，０．２ｍｏｌ／Ｌ焦性没食子酸加入量为２０μｌ（终浓度１０ｍｍｏｌ／Ｌ）；③线性工作范围为：ＳＯＤ浓度２．２５～１１．２５μｇ／２０μｌ；检测限：ＳＯＤ２．２５μｇ／２０μｌ，灵敏度１．０４；④分析的精密度为：组内合并ＣＶ３．８８％～６．７８％，组间合并ＣＶ５．５５％；⑤分析的准确率为：回收率９０．１０％～１０２．３７％，平均回收率（９４．５１±８．２０）％。结论自动超氧化物歧化酶活性分析仪快速、简便、适用范围较广。     

L—Arg—NO途径对铝抑制大鼠海马CA3区诱发电位的影响

实验在７０只乌拉坦麻醉的ＳＤ大鼠上进行，在海马ＣＡ３区记录诱发的群体锋电位（ＰＳ）探讨了左旋精氨酸一氧化氮（Ｌ－Ａｒｇ－ＮＯ）途径对铝抑制ＰＳ波幅的作用。结果：（１）０．５ｍｏｌ／ＬＡｌＣｌ３注入ＣＡ３区后，ＰＳ幅度较注药前显著减小（Ｐ〈０．０５）。（２）ＣＡ３区内注入０．１和０．３ｍｏｌ／Ｌ硝基左旋精氨酸（ＮＬＡ）能分别加强０．２５和０．５ｍｏｌ／ＬＡｌＣｌ３的抑制效应，（３）ＣＡ３区注入０．３     

栗鼠圆窗置氯化钠高渗液对耳蜗血流和内耳电生理的影响

目的：研究０．５ｍｏｌ／Ｌ和６．１ｍｏｌ／Ｌ高渗ＮａＣｌ溶液置栗鼠圆窗对内耳电生理和耳蜗血流的影响。方法：动物全身麻醉,暴露贺窗,分别放置０．５ｍｏｌ／Ｌ１．５ｍｏｌ／Ｌ和６．１ｍｏｌ／Ｌ的ＮａＣｌ溶液棉片,观察２ｈ内听神经复合动作电位阈值况。结果：０．５ｍｏｌ／Ｌ,１．５ｍｏｌ／Ｌ和６．１ｍｏｌ／Ｌ三种ＮａＣｌ溶液分别可提高ＣＢＦ７．６％,２４．５％和３５．３％,０．５ｍｏｌ／Ｌ的ＮａＣｌ溶液对     

人血浆萘哌地尔浓度的高效液相色谱法测定

目的 研究人血浆萘哌地尔的高效液相色谱法（ＨＰＬＣ）测定。方法 血浆样品依次用６ｍｏｌ／ＬＨＣｌ，ＮａＯＨ处理，乙醚提取２次，色谱分析柱为粒径５μｍ，填料为ＥｃｏｓｐｈｅｒｅＣ１８，内标为美托洛尔，流动相为甲醇－乙腈－水－０．０２ｍｏｌ／ＬＫＨ２ＰＯ４（５５：２０：２２：３）ｐＨ４．１０。荧光检测器激发波长２９０ｎｍ，发射波长３４０ｎｍ。结果 最低检测浓度为１．０ｎｇ／ｍｌ天内及天间的变异系数为１     

58例梗阻性黄疸红细胞脆性分析

应用两管法测定红细胞溶血率，对５８例梗阻性黄疸患者进行红细胞脆性分析。发现梗阻性黄疸状态下（胆红素＞３７０μｍｏｌ／Ｌ）约有２０％红细胞的脆性升高，于０．３４５％ＮａＣｌ溶液中红细胞溶破率为９２．５±６．３％，与正常对照组相比Ｐ＜０．０５。黄疸减低后５天（胆红素＜１３０μｍｏｌ／Ｌ）红细胞脆性与正常对照组相比Ｐ＞０．０５，于０．３４５％ＮａＣｌ溶液中红细胞溶破率为７９．７±８．６％。     

用磷酸三丁酯从H_2SO_4－NaCl介质中萃取Ga（Ⅲ）

利用正交试验法探讨了磷酸三丁酯（ＴＢＰ）萃取Ｇａ~（３＋）时ＴＢＰ、Ｈ_２ＳＯ_４及ＮａＣｌ浓度对萃取率的影响。结果表明：在１～１．５ｍｏｌ·Ｌ~（－１）Ｈ_２ＳＯ_４及２５０ｇ·Ｌ~（－１）ＮａＣｌ介质中用（ＴＢＰ）＝１５％～２０％萃取Ｇａ~（３＋）时可得９０％以上萃取率。提示：此法优于具有污染性的ＨＣｌ法。     

海风藤酚,甲基海风藤酚,海风藤醇A和海风藤醇B对兔血小板…

海风藤酚、甲基海风藤酚、海风藤醇Ａ和海风藤醇Ｂ均有抑制血小板激活因子（ＰＡＦ）诱导的兔血小板聚集作用，ＩＣ５０分别为１７．９、９．２、１４２．０、１４．０μｍｏｌ·Ｌ＾－１。经阿司匹林（ＡＳＡ）、磷酸肌酸／磷酸肌酸激酶（ＣＰ／ＣＰＫ）预处理的兔洗涤血小板，甲基海风藤酚和海风藤醇Ｂ仍能抑制ＰＡＦ诱导的兔血小板聚集，ＩＣ５０为９．０、１６．５μｍｏｌ·Ｌ＾－１。同时，４种成分对于二磷酸腺苷由１．５９ｎ     

白屈菜赤碱对PC12细胞乙酰胆碱诱发电流的快速抑制作用

采用全细胞膜片钳技术研究蛋白激酶Ｃ（ＰＫＣ）选择性抑制剂白屈菜赤碱（ＣＨＴ）对ＰＣ１２细胞上乙酰胆碱（３０μｍｏｌ·Ｌ－１）诱发电流（ＩＡＣｈ）的影响。研究表明ＣＨＴ（０．１～１０μｍｏｌ·Ｌ－１）预温流细胞５ｍｉｎ可使ＩＡＣｈ峰值受抑制,此作用呈浓度依赖性,可逆性和非电压依赖性。ＣＨＴ（５μｍｏｌ·Ｌ－１）温育细胞６ｍｉｎ内对ＩＡＣｈ的抑制作用呈时间依赖性。通过微电极将更为有效的ＰＫＣ抑制剂ＰＫＣＩ１９～３１（０．１～５μｍｏｌ·Ｌ－１）透析入细胞内以阻断ＰＫＣ,并不影响ＣＨＴ抑制ＩＡＣｈ的作…     

大鼠主动脉α_1肾上腺素能受体激活引起Ca~（2＋）内流的特性

大鼠主动脉α_１肾上腺素能受体激活引起Ｃａ~（２＋）内流的特性南通医学院药理学教研室徐济良等在大鼠主动脉平滑肌标本上，钙通道阻断剂──硝苯吡啶（０．５μｍｏｌ·Ｌ－１）能完全阻断由ＫＣｌ（１００ｍｍｏｌ·Ｌ－１）引起的血管收缩和４５Ｃａ内流，并部分抑制...     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.