Antagonistic Effect of Poria Cocos on Ototoxicity of Kanamycin in Guinea－Pigs

ＡｎｔａｇｏｎｉｓｔｉｃＥｆｆｅｃｔｏｆＰｏｒｉａＣｏｃｏｓｏｎＯｔｏｔｏｘｉｃｉｔｙｏｆＫａｎａｍｙｃｉｎｉｎＧｕｉｎｅａ－ＰｉｇｓＬＩＵＹａｏ－ｃｈｕｎ（刘耀春）；ＬＩＵＧｕｉ－ｙｉｎｇ（刘桂英）；ＬＩＵＲｕ－ｌｉｎ（刘儒林）；ＣＨＥＮＧＸｉｕ－...     

Effect of Dietary Administration of Lathyrus sativus Pulse on Intestinal Biochemical Parameters in Normal and Scorbutic Guinea Pigs

Objective In order to investigate that ascorbic acid deficiency is responcible for lathyrus toxicity, the effect of dietary feeding of lathyrus pulse in normal and scorbutic guinea pigs for 3 months, on intestinal biochemical parameters was undertaken. Methods The intestinal brush border membrane (BBM) marker and xenobiotic metabolising enzymes (XME) were assayed. Results Exposure to 80% lathyrus alone and in scorbutic conditions showed significant inhibition of alkaline phosphatase (28%-30%), sucrase (19%) and γ-glutamyl transpeptidase (GGT) (15%-27%) enzymes, while Ca2 -Mg2 -ATPase was significantly inhibited (38%) in scorbutic plus lathyrus treated group. The phase I XME (AHH) remained unchanged while the phase II enzyme glutathione-S-tranferase (GST) was significantly decreased (20%-22%) in lathyrus and scorbutic plus lathyrus treated groups. Quinone reductase (QR) activity was found to be significantly decreased in lathyrus exposed group (20%). The intestinal biomarker contents including hexose (2     

Effect of Electroacupuncture on Reperfusion Ventricular Arrhythmia in Rat

Clinically,heartthrombolytictreatmentandopen heartsurgerypossiblygiverisetosevereven triculararrhythmia,evenlethalarrhythmia.Therefore,thesearchforpreventingtheheartfromreperfusion inducedmalignantarrhythmiashasbeenatopicofimmenseinterest.Thereisalargebodyofevidencesupportingthatelectroacu puncture(EA)at"Neiguan"acupointhasastrongprotectiveeffectonischemicmyocardium[1].OurpreviousstudyfoundthatEAatthe"Neiguan"and"Lingdao"acupointsincreasedthethresholdofventricularfibrillationinratswithacute…     

An Analysis of Therapeutic Effect of Drug Acupoint Application in 209 Cases of Allergic Asthma

Both therapies of traditional crude herb moxibustion and drug acupoint application were used in 209cases of allergic asthma to compare their long-term and short-term therapeutic effects and to analyzethe relationship between clinic therapeutic effects of both therapies and differential types of thedisease.The results showed that the short-term total effective rate in the group of drug acupointapplication was higher than that in the group of traditional crude herb moxibustion,the therapeuticeffects of drug acupoint application being closely related to differential types.Analysis also shows thebest short-term therapeutic effect was in the type of heat in the lung while the poorest effect in thetype of deficiency of the kidney-yang.     

Skin Allergy of Guinea Pigs to Mosquito Bites

Dynamic changes of local skin reactions and histopathology in guineapigs exposed to repeated bites of Aedes albopictus under controlled laboratoryconditions for 3 months are reported.It has been found that cutaneous basophilhypersensitivity(CBH)may occur in guinea pigs due to mosquito bites,and as yet,it has not been reported in medical literature.     

Protective Effect of Electroacupuncture and Ischemic Preconditioning on the Circulatory Function in Pigs with Ischemia/Reperfusion Myocardial Injury

Objective: To investigate the effects of electroacupuncture and ischemic preconditioning (IPC) on circulatory function in pigs with myocardial ischemia/reperfusion injury. Method: Eighteen pigs with myocardial ischemia/reperfusion injury were randomly allocated into three groups, 6 in each. Group I was the control group, group II was the group that received IPC, and group III was that received both electroacupuncture and IPC. Blood malondialdehyde (MDA), superoxide dismutase (SOD), creatine phos-phokinase (CPK) and its isoenzyme (CK-MB), coronary artery flow and myocardial heat-shock protein (HSP) mRNA expression were detected for evaluation. Results: After treatment, the MDA content was decreased and SOD activities increased significantly in the acupuncture and IPC group compared with the control group (P<0. 05 respectively). The levels of CPK, CK-MB at 20, 60 min after reperfusion were significantly higher than those before treatment, but the levels in group III and group n were remarkably lower than     

Observation on the Effect of Electroacupuncture in Treating Maxillofacial Hemangioma

Accordingtotheclinicalmanifestationofangioma,especiallythehistopathologiccharacteristicsofvascularen dotheliumcellsandthebiologicalfeatures,angiomaisclassifiedtohemangiomaandvascularmalformationsbyMullikenandGlowacki.Thisclassificationissignificantlyinstructiveforclinicaldiagnosis,evaluationoftreatmentandprognosisofthedisease.Mosthemangiomalikecapil laryhemangioma,strawberryangioma,etc.couldself remitspontaneouslyandnospecialtreatmentisneeded.Butthevascularmalformationssuchascavernousan gioma…     

Effect of Corocinnarine on Physiological Characteristics of Guinea Pig Papillary Muscles

In our experiment it was found that,in guinea pig papillary muscles,8205 de-creased the automaticity and prolonged the functional refractory period(FRP)as well asdecreased the contractile amplitude dose-dependently,but exerted no effect on the excitabili-ty.The dose-response curves of isoproterenol shifted to the right in parallel manner,inaccordance with competitive antagonistic,but the effect of 8205 was much less than thatof propranolol.Dose-response curves of CaCl_2 shifted to the right non-parallelly.The re-sults indicated that the mechanism of anti-arrhythmia of 8205 might be related to theCa~(2+)-antagonism.     

Effect of Cardiopulmonary Bypass on Pulmonary Function in Infants

~~Effect of Cardiopulmonary Bypass on Pulmonary Function in Infants     

Study on Effect of Reinforcing Kidney and Invigorating Spleen Principle on Severe Asthma

Ｓｔｕｄｙ　ｏｎ　Ｅｆｆｅｃｔ　ｏｆ　Ｒｅｉｎｆｏｒｃｉｎｇ　Ｋｉｄｎｅｙ　ａｎｄ　Ｉｎｖｉｇｏｒａｔｉｎｇ　Ｓｐｌｅｅｎ　Ｐｒｉｎｃｉｐｌｅ　ｏｎ　Ｓｅｖｅｒｅ　ＡｓｔｈｍａＸＵＤｅ－ｓｈｅｎｇ，ＳＨＥＮＺｉ－ｙｉｎ，ＷＡＮＧＷｅｎ－ｊｉａｎ，ｅｔ...     

Protective Effect of Jiechangning (结肠宁) Decoction in Treating Experimental Ulcerative Colitis in Guinea Pigs

Ulcerative colitis (UC) is a recurrent inflam matory process of unknown etiology, which maybe maintained by autoimmune processes. Alongwith the alterations of our living style and diets,better health care, improved level of diagnosis andincreasing awareness of the disease, UC incidenceis rapidly rising in China(1). Aminosalicylatesagents, corticosteroids, immunosuppressants, andimmunomodulators are the main available medicalagents for treating it at present. …     

Co-culture of Mouse Embryos with Oviductal Cells of Guinea Pigs and Mice

Oviductal cell cultures were prepared from mice or guineo pigs at different days of estrus eycte and polarized or unpolarized mouse oviductal cells on the development of 1-cell mouse embryos in co-culture system were investigated. One-cell mouse embryos in co-culture with mouse metestrus and guinea pig Day 1 after ovulation had better development of embryos ( 73% and 79% of morula blastocysts, respectively). Co-culture of embryos with oviductal cells was improved when progesterons was added to the medium. Supplementing the co-cutture media with 20 ng/ ml estradiol-17β decreased the percentage of embryos forming blastocyst. The results showed that there was a very tow species specificity for oviductat cells and guinea pig oviductal cells. This provides a good possibitity for development of mouse embryos. The results also showed that good development for the 1-cell mouse embros is not cell polarity dependent.     

Effect of Imidazopyridine a New Cardiotonic Agent on Isolated Guinea Pig Atria

The cardiotonic activity of imidazopyridine(MD),a Chinese-madeproduct of sulmazol(AR-L 115 BS),was verified and compared withthat of isoprenaline(ISO)on isolated guinea pig atria.The electri-cally-paced left atria were used to determine their positive ino-tropic action,while the spontaneously beating right atria were usedto determine their positive chronotropic action.AS a result,MD showed a strong positive inotropic actioncomparable to that of ISO,and some positive chronotropic actionwhich,however,was much weaker than that of ISO.The positiveinotropic action of a single dose of MD developed quite slowly andincreased gradually in a long time,whereas the action of ISOdeveloped so steeply that its peak effect was reached within a fewminutes.Propranolol could significantly block both inotropic andchronotropic actions of ISO but scarcely those of MD.This suggeststhat MD is not a β_1-receptor agonist.The experimental resultsdescribed above are similar to those reported abroad.     

Effects of Hypoxia on Calmodulin Levels of Lung Tissues and Small Pulmonary Arterial Walls in Young Pigs

The effects of chronic hypoxia on calmodulin levels of lung tissues and small pulmonary ar-terial walls were studied in young pigs, The tissue specimens of hypoxic animals were obtained underhypoxic conditions. The following results were collected:(1) The swine exposed to chronic intermittent hypoxia showed a significant pulmonary pressor re-sponse at a simulated high altitude of 4000 m.(2) A higher level of calmodulin was found in the lung tissues of chronic hypoxic animals. It maybe related to the increased release of some vaosactive substances from pulmonary non-muscularcalls.(3) No significant difference of calmodulin level of small arterial walls was demonstrated between theexperimental animals and the control.The findings suggest that pulmonary vasoconstriction due to hypoxia is not likely to be associatedwith obvious change in calmodulin level in the smooth muscle of blood vessels.     

TNF-α and IL-8 of the Patients with Allergic Asthma

The levels of serum TNF-α and IL-8 in the patients with allergic asthma during acute attack period and remission period, and the effects of glucocorticoid (GC) on them were investigated. By using ELISA, the levels of TNF-α and IL-8 were detected in the healthy volunteers (group C, n=40), the patients with allergic asthma (n=40) during acute attack period (group A) and remission period (group B) and those taking GC for a week (n=28). The results were compared among them. It was found that the levels of TNF-α and IL-8 in group A were higher than in group B and group C. In the patients subject to GC therapy, the levels of TNF-α and IL-8 were decreased as compared with those in group A. In group B, the level of TNF-α was higher than in group C, but there was no significant difference in the level of IL-8 between group B and group C. It was concluded that the inflammatory cytokines, TNF-α and 11.-8, played important roles in the bronchus allergic inflammation. GC could reduce the levels of serum TNF-α and IL-8 to exert the anti-inflammatory effects.     

Pathological Observation on the Acoustic Trauma Caused by Cannonade Explosion in Guinea Pigs

Animal experiments demonstrated that severe acoustic trauma of themiddle and inner ears occurred after cannonade explosion within a dis-tance of about 8 metres.Among the 60 ears of 30 guinea pigs,57 showedrupture of eardrums,32 had destruction of ossiele-chains,while 47 hadaccumulation of blood and exudates in the middle ears;a gradient of in-jury of the cochlea was also observed,minimal damage occurred in 3,increased in 41,and maximal in 16.The injuries occurred chiefly in thefirst and second turns,but the third and fourth turns were also involvedin 21.The results coincide with the clinical audiometry curve of thepatients who suffered from explosive acoustic trauma.     

A Study on Protective Action of Astragalus Injection(黄芪注射液)in Ouabain-Induced Cardiac Toxicity of Guinea Pigs

ＡｓｔｒａｇａｌｕｓＩｎｊｅｃｔｉｏｎ（ＡＩ）ｈａｓｂｅｅｎｗｉｄｅｌｙｕｓｅｄｔｏｔｒｅａｔｃｈｒｏｎｉｃｃｏｎｇｅｓｔｉｖｅｈｅａｒｔｆａｉｌｕｒｅ（ＣＨＦ）．Ｒｅｃｅｎｔｌｙ,ＹＡＮＧｅｔａｌ．ｈａｖｅｒｅｐｏｒｔ ｅｄｔｈａｔＡＩｗａｓｃｌｉｎｉｃａｌｌｙｅｆｆｅｃｔｉｖｅｉｎｄｅｃｒｅａｓｉｎｇｔｈｅｉｎｃｉｄｅｎｃｅｏｆｄｉｇｉｔａｌｉｓｍ（1）．ＡｌｔｈｏｕｇｈＡＩｈａｓｂｅｅｎｃｌａｉｍｅｄｔｏｉｎｈｉｂｉｔｄｉｇｉｔａｌｉｓｍ,ｉｔｓｍｅｃｈａ ｎｉｓｍｏｆａｎｔｉ ｄｉｇｉｔａｌ…     

The Influence of LHRH, EB and PGF2α on the Development of Ovarian Follicles in Guinea Pigs

Effects of luwinizing hormone releasing hormone ( LHRH), estradiot benzoate (EB), prostaglandin (PGF2α) and the age of animals on the development of ovarian follicles in immature and mature guinea pigs were studied. Administration of 5ng LHRH / hr, 10 times a day, starting from 20-30 days Of age induced the flrst vaginal opening within 4 days, and 5 of the 10 animals had first ovulation. Tire results also show that appropriate doses of EB facilitated the development of ovary. The granulosa cells of guinea pigs at different ages were cocuttured with the pituitart, glands of mature ones in vitro. Results indicate that the responsiveness of granulosa cells of 20-day-guinea prgs to gonadotropins was similar to that of the matured ones. Effects of gonadotrophin on ovulation in hysterectomized and PGF2α or saline-treated animals were tested. Results demonstrate that when PGF2α was given simultaneously with hCG or FSH, 80% of the guinea pigs ovulawd and had fresh, healthy looking corpus tuteum. It is concluded that increased LHRH release from hypothatamus may be the key factor for tire onset of puberty in guinea prgs. EB and PGF2α can influence the development of the ovary.     

Analysis of Time and Frequency Domain of Auditory Brainstem Response in Normal Guinea Pigs

The auditory brainstem response (ABR) was measured for 90 cars of norml gui-nea pigs,from which the mean latency,amplitude and recognition of waves were obtain-ded.The waves were classified into six types,with wave Ⅲ as the main wave which ac-counted for 77.1% of all the waves.The frequency domain of each type was analysed bymeans of digital filtering and auto-regressive (AR)estimation.The main frequency com-position of ABR in the guinea pigs was restricted within 1600 Hz,in which there werethree peaks at AR spectrum,the mean value of the peaks being 107.33 Hz,566.67 Hzand 1076 Hz respectively.The AR spectrograms of all types waves were very similar toeach other.     

Clinical Observation of the Therapeutic Effect of Ginkgo Leaf Concentrated Oral Liquor on Bronchial Asthma

Ｃｌｉｎｉｃａｌ　Ｏｂｓｅｒｖａｔｉｏｎ　ｏｆ　ｔｈｅ　Ｔｈｅｒａｐｅｕｔｉｃ　Ｅｆｆｅｃｔ　ｏｆ　Ｇｉｎｋｇｏ　Ｌｅａｆ　Ｃｏｎｃｅｎｔｒａｔｅｄ　Ｏｒａｌ　Ｌｉｑｕｏｒ　ｏｎ　Ｂｒｏｎｃｈｉａｌ　ＡｓｔｈｍａＬＩＭｉｎｇ－ｈｕａ，ＹＡＮＧＢｅｎ－...