环氧合酶-2在胃癌中的表达及预后意义

目的:研究环氧合酶-2(COC-2)在胃癌、不典型增生及慢性萎缩性胃炎中的表达情况,探讨COX-2表达的预后意义。方法经手术病理证实的早、中期胃癌281例,进行预后随访调查。232例进行免疫组织化学染色。检则COX-2在胃癌及非癌组织中的表达情况,分析其与预后的关系。结果:早期胃癌的5年生存率为93.4％,中期胃癌的5年生存率为59.0％。COX-2在慢性萎缩性胃炎中的表达明显低于不典型增生者(P<0.01),在癌组织中的表达明显高于非癌组织(P<0.05～0.01);COX-2与胃癌淋巴结转移、血管浸润均密切相关(P<0.01);COX-2阳性表达者5年生存率明显低于阴性者(P<0.01)。结论:COX-2在胃癌组织呈过度表达状态,与胃癌的生长和浸润转移关系密切,可以作为反映胃癌生物学行为和判断预后的有效指标。     

血管内皮生长因子在胃癌中表达及其预后意义

目的研究血管内皮生长因子(VEGF)在胃癌组织中表达的临床意义.方法应用抗 VEGF 多克隆抗体的免疫组织化学方法,观察 VEGF 在胃癌组织中的表达,分析 VEGF 表达与不同监床病理因素及预后之间关系.结果 128例胃癌 VEGF 阳性表达率为64.1%;Ⅲ、Ⅳ期病人 VEGF 阳性表达率明显高于Ⅰ期患者(P<0.05);VEGF 阳性表达率与肿瘤的生长方式、浸润深度及淋巴线转移关系之间有显著统计学意义,肿瘤呈浸润型生长(71.8%)或浸及浆膜者(73.5%)明显大于膨胀型生长(52.0%)或无浆膜浸润者(53.3%)(P<0.025),淋巴结转移阳性肿瘤(75.0%)明显大于无淋巴结转移者(50.0%)(P<0.05);此外,在86例术后随访患者中,VEGF 阳性表达肿瘤患者的术后5年生存率明显低于 VEGF 阴性表达肿瘤患者(P<0.05).     

Survivin、COX-2及VEGF在大肠癌中的表达及与肿瘤微血管密度的关系

目的:研究Survivin, COX-2, VEGF和肿瘤微血管密度(MVD)在大肠癌组织中的表达, 探讨其与大肠癌肿瘤血管生成的关系.方法:2007-09/2008-05哈尔滨医科大学附属第二临床医学院内镜下取材的大肠癌、肠息肉及肠炎标本. 所有标本均经病理检查证实诊断. 试验对象共分3组, 分别为大肠癌组织26例, 大肠息肉组织10例, 大肠黏膜慢性炎症组织7例. 采用免疫组织化学方法检测Survivin,COX-2, VEGF和CD34在大肠组织中的表达.结果:Survivin, COX-2与VEGF蛋白在大肠癌组织中阳性表达率分别为76.9%, 80.8%和69.28%, 明显高于大肠息肉组与肠炎组的表达( P<0.01或0.05). 大肠癌组织中MVD(CD34)明显高于大肠息肉组与肠炎组(23.69±9.96 vs13.10±7.05, 10.43±4.24, 均P<0.01). Survivin,COX-2和VEGF蛋白在大肠癌组中的表达与MVD相关(均P<0.05), Survivin和促血管形成因子COX-2, VEGF在大肠癌组织中的表达密切相关(χ2 = 11.18, 4.72, 均P<0.005).结论:Survivin可能通过COX-2, VEGF促进大肠癌肿瘤血管的形成.     

PTEN、COX-2、Survivin在胃癌中的表达及意义

目的 探讨第10染色体同源丢失性磷酸酶张力蛋白(PTEN)、环氧合酶-2(COX-2)、生存素(Survivin)与胃癌发生发展的关系及作用机制.方法 应用免疫组化S-P法检测80例胃癌组织、15例非胃癌胃黏膜组织中PTEN、COX-2、Survivin的表达.结果 80例胃癌组织中42例PTEN蛋白表达明显下降或缺失,且与胃癌TNM分期、淋巴结转移相关(P＜0.05).COX-2在胃癌组织中的阳性率为67.5%,显著高于正常胃黏膜组织(P＜0.05),并相关于胃癌淋巴结转移、TNM分期.Survivin在胃癌组织中的阳性率为70.0%(P＜0.05),和胃癌分化程度、淋巴结转移、TNM分期有相关性(P＜0.05).在PTEN蛋白阴性的胃癌组中,COX-2阳性率为70.37%,显著高于PTEN阳性组(P＜0.05).结论 抑癌基因PTEN在胃癌及胃癌发展过程中表达逐渐下降或缺失,与凋亡抑制基因COX-2可能存在相互作用,协同Survivin参与了胃癌的发展、转移及浸润.     

血管内皮生长因子与血小板衍化内皮细胞生长因子在老年人胃癌组织中的表达

目的　探讨老年人胃癌术前活检标本血管内皮生长因子 (VEGF)和血小板衍化内皮细胞生长因子 (PD ECGF)的表达及其与老年胃癌患者预后的关系。　方法　采用免疫组化法检测 92例老年胃癌VEGF、PD ECGF表达情况 ,并分析它们与胃癌临床病理特征关系及对预后的影响。　结果　VEGF、PD ECGF在胃癌组织的表达明显高于慢性萎缩性胃炎 ,进展期癌的表达又高于早期癌(P <0 0 1) ,VEGF、PD ECGF表达呈显著正相关 (相关系数R =0 4 0 5 4 )。VEGF、PD ECGF的阳性表达随着肿瘤大小、浸润深度、TNM分期的递增而呈上调表达 ,有淋巴结转移、血管癌栓的患者表达也明显高于无淋巴结转移、血管癌栓者 (P <0 0 1)。VEGF、PD ECGF阳性表达者总体生存率明显低于VEGF、PD ECGF阴性表达者 (P <0 0 1) ,VEGF、PD ECGF共同表达者生存率更低 (P <0 0 1)。多因素分析表明 ,淋巴结转移、TNM分期、VEGF的表达是老年人胃癌独立的预后因素。　结论　VEGF与PD ECGF表达呈正相关 ,均与胃癌生长、浸润转移关系密切 ,可作为估计老年人胃癌预后的重要因素。     

胃癌发生过程中Survivin促血管生成作用的研究

目的探讨胃癌发生过程中Survivin促进血管生成的作用。方法用免疫组化方法检测慢性非萎缩性胃炎、胃癌癌前病变、胃癌组织中Survivin的表达及MVD情况,分析Survivin的表达与胃癌临床病理参数之间的关系,分析Survivin的表达与MVD的关系。结果 Survivin蛋白在胃癌组织中的表达率为71.67%,显著高于慢性非萎缩性胃炎的3.33%及胃癌前病变组织的30.00%,三组间差异有统计学意义(P0.05);Survivin蛋白表达与肿瘤分化程度、胃癌局部浸润、淋巴结转移和TNM分期有关(P0.05)。Survivin表达阳性的胃癌组织及癌旁组织中MVD表达高于Survivin表达阴性的胃癌组织及癌旁组织(P0.05)。结论 Survivin的过度表达是胃癌发生过程中的早期事件,可能可以促进胃癌血管形成。     

血管内皮生长因子、微血管密度与食管鳞癌临床病理及预后的关系

为探讨血管内皮生长因子 (VEGF)、微血管密度 (MVD)与食管鳞癌临床病理和预后的关系 ,对 4 4例原发食管鳞癌、6例食管不典型增生及 10例正常人的食管组织石蜡切片进行血管标记和染色 ,检测 VEGF表达及 MVD。结果显示 ,食管鳞癌患者 VEGF阳性表达率为 6 5 .9% (2 9/ 4 4 ) ,VEGF表达与肿瘤大小、分化程度、淋巴结转移及 TNM分期显著相关 ;MVD与分化程度、淋巴结转移、TNM分期明显相关 ;VEGF表达阳性组 MVD(47.34± 11.5 7条 )明显高于阴性组 (33.73± 11.33条 ) ,VEGF表达与 MVD具有相关性 ;VEGF阴性组和阳性组术后 3年生存率有显著差异 ,高 MVD者和低 MVD者术后 3年生存率有显著差异 (P<0 .0 1)。认为食管鳞癌VEGF表达增强 ,MVD增高 ;VEGF表达在食管鳞癌的生长、浸润和转移过程中起重要作用 ;VEGF和 MVD对于判断食管鳞癌预后有重要参考价值     

利用组织芯片技术研究COX-2在胃癌中的表达及其与血管生成的关系

目的:研究COX-2在胃癌中的表达及其与血管生成的关系,探讨其在胃癌转移中的作用及与胃癌病理生物学行为的关系．方法:采EnVision方法检测胃癌组织芯片中 COX-2的表达,用CD34进行微血管内皮细胞染色,计算微血管密度(MVD),分析其相关性．结果:COX-2在胃癌中的表达明显高于正常胃黏膜(P=0．001)．COX-2的高表达与胃癌的转移(P=0．019)和胃壁浸润深度(0．031)呈正相关,与胃癌的组织病理分型无关(P=0．495), 与Borrmann分型无关(P=0．109)组织MVD (65．49±20．64)明显高于正常胃黏膜组织 (36．21±18．47,P=0．001)．MVD值与胃癌的组织病理分型(P=0．003)和转移有关(P=0．043), 与胃癌胃壁浸润深度(P=0．627)和Borrmann 分型(P=0．634)无明显相关性．COX-2表达阳性组的MVD指数明显高于COX-2表达阴性组 (68．59±19．8 vs 25．82±7．76．P<0．05),COX-2 表达与MVD呈正相关(P=0．001)．结论:组织芯片技术对于快速检测胃癌及其他肿瘤的分子病理学改变是一个强有力的工具．COX-2表达可能通过促进血管形成对胃癌的发生、发展起重要作用,其可作为判断预后和指导治疗的有效指标．     

河湟谷地胃癌患者癌组织生长抑素受体2和CCL7蛋白的表达及临床意义

目的研究河湟谷地胃癌患者癌组织及癌旁组织中生长抑素受体(SSTR)2和细胞趋化因子(CCL)7蛋白的表达及与临床病理特征的关系。方法收集河湟谷地新发胃癌患者癌组织及癌旁组织各60例,免疫组化法检测SSTR2和CCL7蛋白的表达情况,分析不同临床病理参数对SSTR2和CCL7阳性表达的影响以及二者表达的相关性。结果 SSTR2在胃癌组的阳性表达率较癌旁组低(P<0.05);CCL7在胃癌组的阳性表达率较癌旁组明显升高,均具有统计学意义(P<0.05)。高、中分化胃癌组中SSTR2的阳性表达率明显高于低分化胃癌组(P<0.05);有浆膜浸润、肿瘤直径大、淋巴结有转移以及TNM分期为Ⅲ~Ⅳ期者的CCL7阳性表达率均远远高于无浆膜浸润、淋巴结无转移以及TNM分期为Ⅰ~Ⅱ期者(P<0.05)。SSTR2和CCL7在胃癌组织中的表达无相关性(P>0.05)。结论 SSTR2的缺失和CCL7的高表达可能促进河湟谷地胃癌的发生。     

乙型肝炎病毒感染对原发性肝细胞癌血管内皮生长因子、环氧合酶-2蛋白表达的影响

目的:探讨乙型肝炎病毒(HBV)感染对肝细胞癌(HCC)中血管内皮生长因子(VEGF)、环氧合酶-2(COX-2)蛋白表达水平的影响和肿瘤血管形成的临床病理意义.方法:利用血清学指标检测40例HCC的HBV感染情况,采用快速免疫组化法检测HCC中VEGF、COX-2的蛋白表达,用抗CD34单克隆抗体显示血管内皮细胞,根据CD34阳性的血管内皮细胞计数测定肿瘤微血管密度(MVD).结果:HBV感染组中VEGF、COX-2蛋白以及MVD的阳性表达率均高于非HBV感染组,差异有显著性意义(P<0.05).VEGF和COX-2表达呈正相关(r=0.618,P<0.01).结论:HBV可能通过上调VEGF、COX-2等血管形成因子表达,共同促进了肿瘤血管的生成,从而促进HCC的生长、浸润和转移.     

Prognostic significance of expression of cyclooxygenase-2 and vascular endothelial growth factor in human gastric carcinoma

AIM: To investigate the role of cyclooxygenase-2(COX-2) and vascular endothelial growth factor (VEGF) in the development of gastric carcinoma and correlation between expression of COX-2 and VEGF and clinicopathologic features in tissues from patients with gastric carcinoma. METHODS: 281 patients with gastric carcinoma who underwent surgical resection between 1990 and 1999 at the First Affiliated Hospital, Anhui Medical University, PRC, were followed up. Expression of COX-2 and VEGF was investigated retrospectively in 232 gastric carcinoma tissues and 60 noncancerous specimens by using immunohistochemistry. RESULTS: The 5-year survival rates of early gastric carcinoma (EGC) and advanced gastric carcinoma (AGC) were 93.4 % and 59.0 %, respectively. Survival time was highly correlated with lymph node metastasis, vascular invasion, depth of invasion and treatment with chemotherapy. Compared with paired noncancerous tissues, expression of COX-2 and VEGF and microvessel density (MVD) value in carcinoma tissue were significantly higher. The MVD value was much higher in COX-2-positive group and VEGF-positive group than that in COX-2-negative group and VEGF-negative group. Expression of COX-2 and VEGF, as well as MVD value were highly correlated with lymph node metastasis and vascular invasion. The 5-year survival rate of patients with expression of COX-2 or VEGF was significantly lower than that of patients without COX-2 or VEGF expression. Multivariate analysis revealed that VEGF overexpression, lymph node metastasis, COX-2 overexpression, depth of invasion and vascular invasion were all independent prognostic factors of gastric carcinoma. CONCLUSION: Overexpression of COX-2 and VEGF in patients with gastric carcinoma can enhance the possibility of invasion and metastasis, implicating a poor prognosis. They may serve as the fairly good prognostic factors to indicate biologic behaviors of gastric carcinoma.     

Cyclooxygenase-2 overexpression correlates with vascular endothelial growth factor expression and tumor angiogenesis in gastric cancer

Angiogenesis is a key prerequisite for the successful establishment, growth, and dissemination of tumors. Vascular endothelial growth factor (VEGF) has a potent angiogenic activity and cyclooxygenase-2 (COX-2) promotes angiogenesis by modulated production of angiogenic factors including VEGF. The current study was designed to investigate the possible roles of COX-2 and VEGF in gastric cancer angiogenesis. In this study, we conducted an immunohistochemical investigation of COX-2 and VEGF expression in 97 patients with gastric cancer. To assess tumor angiogenesis, microvessel density (MVD) was determined by CD34 immunohistochemical staining. Expression of COX-2 and VEGF in gastric cancer tissues, was demonstrated in 63.9% and 75.3% of cases, respectively. The expression of COX-2 correlated significantly with VEGF expression. High MVD was significantly associated with depth of tumor invasion and poor survival. The mean MVD value of VEGF positive tumors was 79.8 +/- 32.0 and significantly higher than that of VEGF negative tumors. The mean MVD value of COX-2 positive tumors was 77.9 +/- 29.9 and not significantly higher than that of COX-2 negative tumor. The mean value of MVD in tumors positive for both COX-2 and VEGF was significantly higher than that in tumors negative for both. However, there was no correlation between COX-2 or VEGF expression and various clinicopathological features including patient survival. These results suggest that COX-2 may play an important role in carcinogenesis by stimulating tumor angiogenesis in concert with VEGF in human gastric cancer.     

Overexpression of cyclooxygenase-2 and tumor angiogenesis in human gastric cancer

BACKGROUND/AIMS: Cyclooxygenase-2 (COX-2) protein is overexpressed in various cancers, including esophageal, gastric, colon, and pancreatic. To better comprehend the role of COX-2 in gastric cancer, especially with regard to angiogenesis, we investigated COX-2 and vascular endothelial growth factor (VEGF) expression and microvessel density (MVD) in 108 patients with gastric cancer. METHODOLOGY: We used immunohistochemical analysis of formalin-fixed tissues of gastric cancer. RESULTS: Expression of COX-2 showed diffuse staining in the cytoplasm of tumor cells, however, no staining in normal epithelial cells. Of the 108 tumors examined, 71 (65%) were positive for COX-2 expression, the VEGF-positive cases numbered 43 of 108 cases (39.8%). The intensity of COX-2 expression did not correlate with any clinicopathological characteristics. The positive rate of VEGF expression in COX-2-positive cases was significantly higher than in COX-2-negative ones (47.9% vs. 24.3%, P<0.05). MVD in COX-2-positive cases was significantly higher than in COX-2-negative ones (22.0+/-7.8 vs. 18.5+/-7.5/1 mm2; P<0.05). CONCLUSIONS: Our study provides evidence that COX-2 is closely related with angiogenesis.     

CD34和VEGF在肝癌组织中的表达及与肿瘤血管生成的相关性

目的探讨CD34和血管内皮生长因子（VEGF）在肝细胞肝癌（HCC）组织中的表达及微血管密度（MVD）的临床病理意义。方法应用链霉素扰生物素蛋白-过氧化物酶法（S—P法）对50例HCC患者进行肿瘤血管生成免疫组织化学检测。对CD34阳性血管进行MVD计数．对VEGF进行半定量计数，并分析与HCC的临床病理特征的关系及意义。结果CD34在HCC组织中呈广泛、窦隙状表达，MVD值在有汇管区癌栓和肝内转移者高于无汇管区癌栓和无肝内转移者（P〈0．05），VEGF表达的阳性率在有汇管区癌栓和肝内转移者明显高于元汇管区癌栓及无肝内转移者（P〈0．05）。MVD值在VEGF阳性组和阴性组之间差异有显著性（P〈0．05）。结论HCC中MVD值和VEGF阳性表达率明显增高，由自分泌和旁分泌产生的VEGF通过促进HCC肿瘤血管生成而促进HCC的生长和转移。     

Microvessel density is a prognostic marker of human gastric cancer

INTRODUCTIONGastric cancer is one of the most frequent and lethal malignancies worldwide, especially in Eastern Asia including China, and the 5-year survival rate is only about 20%[1]. A recent research has shown an increasing trend of gastric cancer mortality in China in the past 20 years, especially in rural areas and among aged people[2]. To date, the treatment outcome of this common malignancy is still not satisfactory. One major difficulty in the diagnosis and treatment of gastric c…     

Prognostic significance of vascular endothelial growth factor expression and microvessel density in carcinoma of ampulla of Vater

BACKGROUND/AIMS: To investigate whether the expression of vascular endothelial growth factor (VEGF) and microvessel density (MVD) are of prognostic significance in ampullary carcinoma. METHODOLOGY: Twenty-two resected tumor specimens from patients with ampullary carcinoma were immunohistochemically stained for VEGF and CD34 (surrogate for vessels) by streptavidin-peroxidase method. RESULTS: Expression of VEGF in tumor tissue was found in 50% of patients. The mean MVD for entire group was 26.4 +/- 12.8. A significantly higher MVD was observed in the tumors with positive VEGF expression (35.0 +/- 9.6) compared with that of negative VEGF expression (17.7 +/- 9.3) (p<0.01). The expression of VEGF and MVD were closely related lymph node status and tumor TNM stage. The positive expression rate of VEGF and the average MVD in patients with lymph node metastases were 85.7% and 33.1 +/- 10.8 respectively, which were significantly higher than those in patients without lymph node metastases (33.3% and 22.8 +/- 11.8 respectively) (p<0.05). The positive expression rate of VEGF and the average MVD in patients with stage III and were 75% and 36.3 +/- 8.4 respectively, which were significantly higher than those in patients with stage I (25% and 18.4 +/- 10.1 respectively) or stage II (50% and 23.8 +/- 13.4 respectively) (p<0.05). The Kaplan-Meier survival curves showed that the 3-year survival rate for patients with positive VEGF expression or a high MVD (9.1% and 10% respectively) were lower than those in patients with negative VEGF expression or a low MVD (63.64% and 58.33% respectively) (p<0.05). CONCLUSIONS: VEGF is positively correlated with MVD in ampullary carcinoma. VEGF and angiogenesis may play an important role in lymph node metastasis and progression of ampullary carcinoma. VEGF and MVD appear to be important prognostic predictor in patients with ampullary carcinoma.     

Correlation between expression of cyclooxygenase-2 and angiogenesis in human gastric adenocarcinoma

AIM: To evaluate the expression of cyclooxygenase (COX2) and the relationship with tumor angiogenesis and advancement in gastric adenocarcinoma.METHODS: Immunohistochemical stain was used for detecting the expression of COX-2 in 45 resected specimens of gastric adenocarcinoma; the monoclonal antibody against CD34 was used for displaying vascular endothelial cells, and microvascular density (MVD) was detected by counting of CD34-positive vascular endothelial cells. Paracancerous tissues were examined as control.RESULTS: Immunohistological staining with COX-2-specific polyclonal antibody showed cytoplasmic staining in the cancer cells, some atypical hyperplasia and intestinal metaplasia,as well as angiogenic vasculature present within the tumors and prexisting vasculature adjacent to cancer lesions. The rate of expression of COX-2 and MVD index in gastric cancers were significantly increased, compared with those in the paracancerous tissues (77.78 vs 33.33 %, 58.13±19.99 vs 24.02±10.28, P＜0.01, P＜0.05, respectively). In 36 gastric carcinoma specimens with lymph node metastasis, the rate of COX-2 expression and MVD were higher than those in the specimens without metostasis (86.11 vs 44.44 %,58.60±18.24 vs 43.54±15.05, P＜0.05, P＜0.05, respectively).The rate of COX-2 expression and MVD in the specimens with invasive serosa were significantly higher than those in the specimens without invasion to serosa (87.88 vs 50.0 %,57.01±18.79 vs42.35±14.65, P＜0.05, P＜0.05). Moreover,MVD in COX-2-positive specimens was higher than that in COX-2-negative specimens (61.29±14.31 vs 45.38±12.42,P＜0.05). COX-2 expression was positively correlated with MVD (r=0.63, P＜0.05).CONCLUSION: COX-2 expression might correlate with the occurance and advancement of gastric carcinoma and is involved in tumor angiogenesis in gastric carcinoma. It is likely that COX-2 by inducing angiogenesis can be one of mechanisms which promotes invasion and metastasis of gastric carcinoma. It may become a new therapeutic target for anti-angiogenesis.     

Relationship between the expression of iNOS,VEGF,tumor angiogenesis and gastric cancer

AIM：To in vestigate the relationship between the expression of inducible nitric oxide synthase(iNOS),vascular endothelial growth factor(VEGF),the microvascular density(MVD)and the pathological features and clinical staging of gastric cancer.METHODS:Immunohistochemical staining was used for detecting the expression of iNOS and VEGFin46resected specimens of gastric carcinoma;the monoclonal antibody against CD34 was used for displaying vascular endothelial cells,and MVD was detected by counting of CD34-positive vascular endothelial cells.RESULTS:Of 46resected specimens of gastric carcinoma,the rates of expressions of iNOS and VEGF were 58.70%and76.09%,respectively,and MVDaveraged55.59&#177;19.39,Judged by the standard TNM criteria,the rate of expression of iNOS in stageⅣ（84.46%)was higher than those in stageⅠ,Ⅱ,Ⅲ（Fish exact probabilities test,P=0.019,0.023and 0.033,respectively);the rates of expression of VEGFin stage Ⅲ,Ⅳ（76.0%,92.31%,respectively)were higher than those in stageⅠ,Ⅱ（Fis exact probabilities test,P=0.031,0.017,0.022and0.019).MVDs in stageⅢ,Ⅳ（64.72&#177;14.96,67.09&#177;18.29,respectively)were higher than those in stageⅠ,Ⅱ(t\2.378,4.015,2.503and2.450,P&lt;0.05,P&lt;0.001,P&lt;0.001,P&lt;0.05,respectively),In37gastric carcinoma specimens with lymph node metastasis,MVD(68.69&#177;18.07)and the rates of expression of iNOS and VEGF(70.27%,83.78%,respectively)were higher than those in the specimens with absence of metastasis(t=2.205,X^2=6.3587,X^2=6.2584,P&lt;0.01,P&lt;0.05,P&lt;0.05,respectively),MVD and the expressions of iNOS and EGF were not correlated to the location,size or grade of tumor,nor with the depth of invasion of tumor;MVDs in the positive iNOS and VEGF specimens(59.88&#177;18.02,58.39&#177;17.73,repectively)were higher than those in the negative iNOS and VEGF specimens(X^2=6.3587and 6.1574,P&lt;0.05,P&lt;0.05,respectively);thus the expressions of iNOS and VEGF was correlated to MVD,but the expression of iNOS was not correlated to that of VEGF,In addition.of the 46 surviving patients,the 5-year survival rate of patients with positive iNOS or VEGF tumors was significantly less than that of patients with negative iNOS-or VEGF tumors(X^2=4.3842and 5.4073,P&lt;0.05,P&lt;0.05.respectively).CONCLUSION:The expressions of iNOS and VEGF are colosely related to tumor angiogenesis,and are involved in the advancement and the lymph node metastasis;thusMVD and the expressions of iNOS and EGF may serve indexes for evaluating staging of gastric carcinoma and forecasting its risk of metastasis,which will help establish a comprehensive therapeutical measure of post-operative patients and provide a new approach to tumor therapy.