GA—50医用器械消毒液对口腔门诊常用器械的消毒效果观察

用ＧＡ－５０医用器械消毒浸泡口腔门诊常用医疗器械，并对其杀菌效果和乙型肝炎表面抗原ＨＢｓＡｇ破坏试验进行了观察。结果表明：该消毒液作用５－１０ｍｉｎ，对金黄色葡萄球菌的杀灭率达９９．９％．任用－３ｈ，对枯草芽胞杆菌的杀灭率达９９．９％．作者１０－３０ｍｉｎ，对ＨＢｓＡｇ抗原有破坏作用该消毒液连续使用３周，仍能达到合格的要求。     

污染HBsAg的口腔器械消毒方法的实验研究

目的：探讨一种能在较短时间内灭活ＨＢＶ的消毒方法。方法：采用不同浓度的戊二醛擦拭消毒已污染ＨＢｓＡｇ阳性血清的口腔专科常用诊疗器械,用ＥＬＩＳＡ法检测ＨＢｓＡｇ。结果：用２０～３０ｍｌ／Ｌ戊二醛消毒剂擦拭消毒１遍已污染ＨＢｓＡｇ的口腔器械,ＨＢｓＡｇ的灭活率为８％,而采用擦拭消毒３遍的方法,ＨＢｓＡｇ的灭活率为９２％～１００％。结论：选用较高浓度的戊二醛消毒剂擦拭消毒３遍,可以达到灭活ＨＢｓＡｇ的目的。     

我院对病房患者及门诊器械乙肝病毒标志监测结果的报告

作者采用ＥＬＩＳＡ法对颌面外科３０例血清ＨＢｓＡｇ阳性患者，加测了唾液ＨＢｓＡｇ，血清及唾液ＨＢｅＡｇ．抗ＨＢｃ等项目。另对门诊各科４３１件口腔顺械抽样检测了ＨＢｓＡｇ。     

SCD消毒剂浸泡消毒口腔器械的实验研究

笔者选用近年来国内外已开始应用于医学临床的广谱高效消毒剂ＳＣＤ ,并用已污染ＨＢｓＡｇ阳性血清的 6种口腔常用治疗器械进行消毒的实验观察。一、材料和方法1.器械 :机头、口镜、牙钳、镊子、探针、弯盘 ;中和剂ＰＢＳ缓冲液 ;酶标仪ＳＴＬ(ＡＵＳＴＲＩＡ) ;ＨＢｓＡｇ试剂盒 (厦门新创 )。2 .污染ＨＢｓＡｇ的载体制备 :将载体放入装有 10 0ｍｌＨＢｓＡｇ阳性血清 (效价 1:10 0 0 )的烧杯中 ,放置 37 Ｃ温箱内45ｍｉｎ ,取出后待消毒。3.污染ＨＢｓＡｇ载体的消毒 :消毒剂为ＳＣＤ ,浓度为5 0 0ＰＰＭ、10 0 0ＰＰＭ、2 0 0 0ＰＰＭ …     

金星消毒液对口腔科器械消毒效果的初步研究

金星消毒液对口腔科器械消毒效果的初步研究赵勤俞未一孙金竺徐道寅（南京医学院附属口腔医院内科流行病学教研室２１０００９）～～～～～～～～～～～～～～～～～～～～乙型肝炎病毒表面抗原（ＨＢｓＡｇ）阳性的传播，主要是通过血液与唾液。而口腔疾病，医生在诊治时...     

口腔糜烂型扁平苔藓与慢性肝病的临床研究

本研究对３２例口腔糜烂型扁平苔藓患者的肝酶学及病原学进行检测，结果发现，有７例患者（２１．８８％）的血清谷丙转氨酶（ＡＬＴ）和／或血清谷草转氨酶（ＡＳＴ）值升高，且这７例患者中有５例ＨＢｓＡｇ、ＨＢｅＡｇ和抗ＨＢＣ阳性。另有１例患者ＨＢｓＡｇ、ＨＢｅＡｇ和ＨＢＣ阳性。２例患者抗ＨＣＶ阳性，而血清酶水平未见升高。本研究提示对口腔糜烂型扁平苔藓患者，除应注意询问相关病史外，还就常规进行功能和肝炎病毒检     

口腔科器械乙肝病毒消毒测试报告

为了解我科医疗器械目前采取的消毒方法对乙型肝炎病毒消毒的安全性、可靠性,我们对本科５种常用器械进行了乙型肝炎病毒表面抗原（ＨＢｓＡｇ）和ｅ抗原（ＨＢｅＡｇ）的消毒测试,结果如下。材料及方法１．调查对象：选择５套一次性口镜、镊子、探针和５颗车针,将器械...     

唾液对变链粘附作用的影响

用放射性同位素３Ｈ－胸腺嘧啶核苷标记变链Ｓ．ｍｕｔａｎｓＪＢＰ株（血清Ｃ型）及Ｓ．ｓｏｂｒｉｎｕｓ６７１５株（血清ｇ型），观察兔全唾液对这两种细菌在羟磷灰石（ＨＡ）表面粘附的影响．结果发现，ＪＢＰ和６７１５在无唾液包被的ＨＡ上的粘附无明显差异（Ｐ＞０．０５），但经唾液包被ＨＡ后ＪＢＰ粘附量明显多于６７１５（Ｐ＜０．０１），说明唾液成分可促进Ｃ型变链的粘附，但对ｇ型变链的粘附影响不大．     

金星消毒液在口腔科的使用与评价

本文对不同浓度的金星消毒液在不同时间内对金黄色葡萄球菌和ＨＢｓＡｇ灭活的效果进行了实验观察并提出了金星消毒液的合理使用方法及注意事项。     

不同浓度-时间点氢氟酸蚀刻机加工瓷面对瓷与牙釉质的整体粘结抗剪强度的影响

选择３０个氢氟酸（ＨＦ酸）浓度－时间搭配点，测试瓷贴面复合体整体粘结抗剪强度。结果表明：ＨＦ酸蚀刻可增加陶瓷与牙釉质间粘结强度，ＨＦ酸浓度－时间的最佳搭配点为：２．５％－５．０ｍｉｎ；５．０％－５．０ｍｉｎ；７．５％－２．５ｍｉｎ；１０．０％－１．０ｍｉｎ；１５．０％－０．５ｍｉｎ。陶瓷－树脂－牙釉质的粘结破坏形式以混合破坏为主     

Accuracy of Irreversible Hydrocolloid Impression Material Mixed With Disinfectant Solutions

Purpose Irreversible hydrocolloid is capable of resisting contamination by viruses and saliva when the disinfectant iodine or the disinfectant chlorhexidine is added to the water being used to mix the material. This study investigates the accuracy of alginate impressions made with water to which either of the two disinfectants has been added. Materials and Methods A total of 30 casts were made from an impression of a master cast. Ten impressions were made using water according to the manufacturer's instructions, ten were made using water to which iodine had been added, and ten were made using water to which chlorhexidine had been added. A measuring microscope was used to measure three different dimensions on each cast. Each measurement was repeated three times, and the average was used to compute the mean and standard deviation for each measured dimension. The data were statistically evaluated using a one-way analysis of variance. Results There was no statistical difference in the accuracy of irreversible hydrocolloid impressions mixed with water or with either of two disinfectant solutions. Conclusion Accurate casts can be obtained when either of the two disinfectants are incorporated into the alginate impression material.     

调合消毒法对藻酸盐印模材消毒效果的研究

目的:研究藻酸盐印模材料与消毒液直接调拌对微生物的杀灭效果。方法:用0.25%NaC lO、0.525%Na-C lO、1?、0.5?、0.25?消毒液调拌藻酸钾印模材制作实验样本,以无菌生理盐水调拌相同材料做阳性对照和阴性对照,分别接种枯草芽胞杆菌、大肠杆菌、金黄色葡萄球菌(以下简称金葡萄)、绿脓杆菌并在接种30 m in和60 m in取样,经培养后计算各组样本的CFU数并进行统计学分析。结果:0.25%NaC lO、0.525%NaC lO、1?、0.5?在30 m in和60 m in均能完全杀灭大肠杆菌和金葡菌,对枯草芽胞杆菌的杀灭率分别为43.6%~46.1%、61.5%~65.6%、63.7%~66.4%、50.0%~54.7%,对绿脓杆菌的杀灭率分别为60.9%~60.9%、68.5%~70.7%、72.2%~74.1%、66.3%~66.8%。而0.25?对大肠杆菌、枯草芽胞杆菌、金葡菌和绿脓杆菌的杀灭率分别为53.7%~52.7%、29.3%~35.5%、56.0%~60.1%、35.6%~34.9%。统计学分析表明,0.25%NaC lO、0.525%NaC lO、1?、0.5?对4种微生物各自的杀菌效果没有显著性差异,但显著高于0.25?的消毒效果。结论:0.25%NaC lO、0.525%NaC lO、1?、0.5?可完全杀灭大肠杆菌和金葡菌并有效杀灭枯草芽胞杆菌和绿脓杆菌,可用于口腔印模的消毒。     

过氧化氢银离子消毒剂控制微生物污染的效果检测

目的 研究过氧化氢银离子消毒剂对江苏省口腔医院牙周科的口腔科综合治疗台水路系统的微生物污染的控制效果。方法 选取牙周科4台椅位进行3 d/次的管道消毒,共6 d。消毒前后分别采集三用枪,漱口位,超声洁治机柄出水口的水样10 mL在R2A培养基上进行菌落培养,统计消毒前后菌落密度,数据用SPSS 19.0进行统计分析。结果 消毒前三用枪、漱口位、高速手机3个位点出水的平均菌落密度分别为34425、13116.67、42858.33 CFU/mL,无统计学差异。消毒之后灭菌率在92~100%之间,且三个位点的消毒效果无统计学差异。合格率在消毒结束当天早晨平均为91.67%,但消毒结束后第三天早晨,合格率仅为45.83%。结论 应用过氧化氢银离子消毒剂对口腔科综合治疗台水路系统微生物污染改善效果显著,但持久性方面有欠缺。     

Effect of Incorporation of Disinfectant Solutions on Setting Time, Linear Dimensional Stability, and Detail Reproduction in Dental Stone Casts

Purpose: The aim of this paper was to analyze the influence of incorporation of disinfectants during the cast die stone-setting time. Setting time, linear dimensional stability, and reproduction details on casts were measured.
Materials and Methods: Die stone type IV specimens with disinfection solutions (sodium hypochlorite 1%, glutaraldehyde 2%, chlorhexidine 2%) were incorporated in two concentrations (50%, 100%). The detail reproduction, dimensional stability, and setting time were tested in accordance with ADA recommendations.
Results: Disinfecting solutions promoted an increase in setting time compared to control; sodium hypochlorite was responsible for the highest setting time. The addition of undiluted sodium hypochlorite 1.0% led to contraction during setting, but the groups with 50% diluted sodium hypochlorite 1.0% and undiluted chlorhexidine 2.0% resulted in intermediate values compared to the other groups, thus matching the control. The others did not demonstrate any effect on expansion. For detail reproduction, it was observed that the control group presented results similar to the others, except those where sodium hypochlorite was added.
Conclusions The addition of sodium hypochlorite in both dilutions significantly altered, negatively, all the evaluated properties. But the addition of glutaraldehyde and chlorhexidine did not promote any significant alterations in the evaluated properties.     

Efficacy of Different Disinfectant Systems on Alginate and Addition Silicone Impression Materials of Indian and International Origin: A Comparative Evaluation

Study was planned to evaluate the efficacy of commonly used disinfectants and to study qualitatively and quantitatively the persistence of microflora on the untreated (control group) and the disinfected impression surface after 24 h. Disinfectant systems used were immersion systems like glutaraldehyde, sodium hypochlorite and the ultraviolet chamber. The effect of disinfectant on most commonly used Indian impression materials was carried out in this study and results compared with the most commonly used foreign brands for irreversible hydrocolloid and addition silicone. Impressions were made of 25 healthy volunteers. These were disinfected and incubated in an incubator for 24 h at 37°C for aerobic organisms. The inoculation in nutrient media was done to test the viability of microorganisms that can persist after rinsing and disinfection of the impression surface. The colony forming units were counted and compared with that of control group. Control group of all the impression material samples showed growth of Streptococcus viridans, Diphtheroids, Streptococcus pneumoniae to a greater extent. The growth of Candida albicans, Pseudomonas aerugenosa and Staphyloccus albus was present in all the groups but to a lesser extent. The persistence of the microflora on the impression surface of both the studied brands was similar but the concentration of organisms in the alginate control group was two folds as compared to addition silicone group. Use of ultraviolet chamber gave better results compared to the studied immersion systems. All the disinfection systems were effective in reducing the microbial load with ultraviolet chamber as the most effective.     

Effect of Chemical Disinfectant on the Transverse Strength of Heat-polymerized Acrylic Resins Subjected to Mechanical and Chemical Polishing: An in vitro Study

Aims: To evaluate the effect of chemical disinfectant on the transverse strength of heat-polymerized acrylic resins subjected to mechanical and chemical polishing. Materials and methods: A total of 256 rectangular specimens (65 * 10 * 3 mm) 128 per resin (Lucitone-199 and Acralyn-H) were fabricated. One side of each specimen was not polished and the other was either mechanically (n = 96) or chemically (n = 96) polished and immersed for 10, 30 and 60 minutes in 2% alkaline glutaraldehyde. Mechanically polished (n = 32) and chemically polished (n = 32) control specimens were immersed only in distilled water. The transverse strength (N/mm(2)) was tested for failure in a universal testing machine, at a crosshead speed of 5 mm/min. Data were statistically analyzed using 2-way ANOVA and Student t-test. Results: chemical polishing resulted in significantly lower transverse strength values than mechanical polishing. Lucitone- 199 resin demonstrated the highest overall transverse strength for the materials tested. Heat-polymerized acrylic resins either mechanically or chemically polished, did not demonstrate significant changes in transverse strength during immersion in the disinfecting solution tested, regardless of time of immersion. Conclusion: Lucitone-199 resin demonstrated the highest overall transverse strength for the materials tested and significantly stronger than Acralyn-H with either type of polishing following immersion in 2% alkaline glutaraldehyde. Clinical significance: There is a concern that immersion in chemical solutions often used for cleansing and disinfection of prostheses may undermine the strength and structure of denture base resins. In this study it was observed that, the transverse strength of samples of Lucitone-199 was higher than that of the samples of Acralyn-H. The chances of fracture of the denture made of Lucitone-199 are less than that of dentures made of Acralyn-H. The chemically polished dentures may be more prone to fracture than mechanically polished dentures. Keywords: Heat-polymerized acrylic resins, Chemical disinfection, Mechanical polishing, Chemical polishing. How to cite this article: Sharan S, Kavitha HR, Konde H, Kalahasti D. Effect of Chemical Disinfectant on the Transverse Strength of Heat-polymerized Acrylic Resins Subjected to Mechanical and Chemical Polishing: An in vitro Study. J Contemp Dent Pract 2012;13(3):389-393. Source of support: Nil Conflict of interest: None declared.