儿童单纯性肥胖程度和脂肪细胞因子的关系

目的 探讨儿童单纯性肥胖程度与脂肪细胞因子瘦素、脂联素和抵抗素水平的关系.方法 选取2～6岁单纯性肥胖儿童110例(肥胖组),其中轻度肥胖组40例,中度肥胖组40例,重度肥胖组30例,另选取正常体重健康儿童40例为对照组,检测空腹血清瘦素、脂联素和抵抗素水平,比较脂肪因子的差异.结果 肥胖组血清瘦素、抵抗素水平明显高于对照组(P<0.01),血清脂联素水平明显低于对照组(P<0.01).中度肥胖组血清瘦素、抵抗素水平明显高于轻度肥胖组[分别为(14.3±1.9)μg/L比(7.8±1.5)μg/L与(14.6±2.2)μg/L比(13.1±3.0)μg/L](P<0.01),脂联素水平明显低于轻度肥胖组[(6.7±3.0)mg/L比(9.1±3.5)mg/L](P<0.01);重度肥胖组血清瘦素、抵抗素水平[分别为(20.4±4.7)、(18.3±3.2)μg/L]明显高于中度肥胖组(P<0.01),脂联素水平[(5.1±1.2)mg/L]明显低于中度肥胖组(P<0.01).结论 儿童脂肪细胞因子水平与肥胖程度密切相关,内脏脂肪堆积可能是造成脂肪因子改变的主要原因之一.     

Hcy及 VEGF与妊娠高血压疾病的关系

目的：探讨同型半胱氨酸（ Hcy）、血管内皮生长因子（ VEGF）与妊娠高血压疾病（ HDP）的关系及其临床意义。方法选取浙江省瑞安市人民医院2012年1月至2013年12月收治的800例HDP患者为研究对象,其中妊娠高血压375例,轻度子痫305例,重度子痫120例,另选取同期正常孕晚期妇女800例为对照组,分别采用循环酶法及酶类免疫法测定两组血清Hcy、VEGF水平。结果与对照组相比,HDP组孕妇Hcy水平较高,而VEGF水平较低,差异有统计学意义（ t值分别为4．265和5．426,均P＜0．05）。与妊娠高血压组、轻度子痫组相比,重度子痫组孕妇血清Hcy水平较高,差异具有统计学意义（ t值分别为34．575和15．855,均P＜0．05）,而VEGF水平较低,差异具有统计学意义（t值分别为9．512和6．906,均P＜0．05）。与预后良好组相比,预后差组孕妇血清Hcy水平较高,VEGF水平较低,差异有统计学意义（t值分别为5．448和6．325,均P＜0．05）。结论Hcy、VEGF水平升高与HDP病情发生、进展及转归具有密切的关系,Hcy、VEGF可作为HDP患者预后的评价指标。     

绝经期女性激素水平与动脉粥样硬化的关系

目的 研究绝经期女性激素水平与动脉粥样硬化(AS)的关系.方法 纳入2018年1月-2019年4月于银川市中医医院心血管内科住院的接受冠脉造影的绝经女性142例为研究对象,均行性激素水平检测,包括雌二醇(E2)、睾酮(T)、促卵泡生成素(FSH)、促黄体生成素(LH)水平及E2/T值.所有患者均接受冠脉造影,根据冠脉造...     

尿液中微量元素水平与儿童肥胖相关性分析

目的:探讨学龄前儿童体内微量元素水平与其肥胖之间的关系。方法:以苏州市某幼儿园5~7岁儿童为研究对象,通过测量身高、体质量、BMI指数(包括父母亲)筛选出肥胖儿童,共29人,并选取31名正常儿童作为对照组,检测其尿液中的微量元素含量。利用SPSS17.0软件进行差异性检验和相关性分析。结果:两组儿童微量元素水平差异极其显著(P=0.00),其中肥胖儿童尿液中Fe、Zn、Cu的水平分别为27.9±12.4μg/dl,41.4±21.5μg/dl,1.8±0.6μg/dl,均远远低于对照组(分别为83.7±21.6μg/dl,86.5±19.0μg/dl,51.9±3.9μg/dl),并且其尿液中Fe、Zn、Cu的水平与其BMI指数呈负相关(r0)。肥胖儿童BMI指数与其父母亲的BMI指数均呈正相关(0r1)。结论:尿液中微量元素水平与儿童肥胖之间有着密切联系,BMI指数越大,肥胖越严重,微量元素水平越低。此外,儿童肥胖以及肥胖程度还与其父母体型存在一定联系。     

儿童抽动症与微量元素水平的关系研究

目的 探讨儿童抽动症(TD)与患儿体内铜、锌、铁、钙、镁、铅6种微量元素水平的关系,分析TD的危险因素,以期对今后的临床诊断和治疗提供参考资料.方法 选取2013年1月至2015年1月于保定市儿童医院就诊的196例抽动症患儿作为观察组,随机选取于本院门诊体检的健康儿童200例作为对照组.采用原子吸收分光光度仪,测量两组儿童体内铜、锌、铁、钙、镁5种微量元素水平及铅水平.运用病例对照法进行Logistic二元回归逐步分析TD与上述微量元素关系.结果 TD患儿体内的锌、铁水平均较正常儿童低(t值分别为7.711、2.691,均P<0.05),而铅水平明显比正常儿童高(t=3.596,P<0.05).不同性别的TD患儿锌、铁、铅水平之间的差异均无统计学意义(t值分别为0.911、0.891、0.896,均P>0.05),经Logistic逐步回归分析可知,锌、铁、铅水平是TD发病的相关危险因素(OR值分别为3.012、2.947、5.834,均P<0.05).微量元素铜、钙、镁在正常体检儿童体内的水平与TD患儿体内水平均无显著差异(t值分别为1.894、1.605、0.121,均P>0.05).结论 TD患儿体内锌、铁的水平低于正常儿童,而铅的水平高于正常儿童,提示儿童体内的微量元素失衡是抽动症发生的危险因素.     

孕妇血微量元素与胎儿生长受限的关系分析

目的 探讨西藏拉萨市部分孕妇末梢血微量元素铜、锌、钙、镁和铁含量以及其与胎儿生长和发育的关系,为孕产妇的保健及临床治疗提供依据.方法 采用原子吸收分光光度法检测245例妊娠晚期孕妇(包括胎儿生长受限38例,非胎儿生长受限207例)末梢血铜、锌、钙、镁、铁的含量.结果 非FGR者血铜、锌、钙、镁、铁的含量均在正常值范围,其中钙、铁水平偏低,略高于正常值下限.FGR组锌、铁、钙水平(分别为73.24±30.35μmol/L、1.32±0.36mmol/L、7.23±2.42mmol/L)均低于正常值和非胎儿生长受限组(分别为109.63±27.43μmol/L、1.56±0.34mmol/L、8.82±1.71mmol/L),有显著性差异(t分别为7.39、3.96、4.91,均P<0.05).结论 妊娠晚期母亲锌、钙、铁缺乏与FGR的发生有关,妊娠期应根据检测结果 适量补充.     

OSAS与T2DM糖代谢和胰岛素水平关系的研究

目的 对阻塞性睡眠呼吸暂停综合征(OSAS)和2型糖尿病(T2DM)的糖代谢、胰岛素水平关系进行探究。方法 选取2015年9月1日至2017年3月1日收治的75例OSAS患者设为研究组,另选同期收治的75例非OSAS者设为对照组,检测2组糖代谢水平(FPG、2hPG)、糖化血红蛋白指标和胰岛素水平(FIN、2hINS),分析OSAS与T2DM之间的关系。结果 经检测后,对比2组糖代谢、糖化血红蛋白指标和胰岛素水平,发现研究组均比对照组高(P<0.05);研究组中OSAS重度者的糖代谢值和胰岛素值均比OSAS轻度者与OSAS中度者明显升高(P<0.05)。结论 OSAS在一定程度上能引发异常糖代谢与T2DM,紊乱程度越大,糖代谢、胰岛素值越高,可见OSAS和T2DM在糖代谢和胰岛素水平方面呈正相关关系。     

Diet and carcinogen alter luminal butyrate concentration and intracellular pH in isolated rat colonocytes

A 2 × 2 factorial experiment was conducted to examine the effects of two different dietary fibers and carcinogen treatment on colonic luminal short‐chain fatty acid (SCFA) concentrations and intracellular pH (pH_i) in rats. Twenty‐four male Sprague‐Dawley rats were divided into four groups, injected with a carcinogen [azoxymethane (AOM)] or normal saline (Sal), and fed one of two diets differing only in the type of dietary fiber [cellulose (Cell) or pectin (Pect)]. After 38 weeks of consuming these diets, the rats were euthanized, luminal contents were collected for analysis of SCFA concentrations, and colonocytes were isolated from the proximal and distal colon for subsequent determination of pH_i Changes in pH_i after the addition of exogenous sodium butyrate to the culture medium were also tested. The highest concentrations of SCF As were produced by the control rats (saline injected) consuming the pectin diet. Luminal butyrate concentrations were reduced in three of four colonic segments of carcinogen‐injected groups [proximal and distal cellulose (Prox Cell and Dist Cell) and distal pectin (Dist Pect)] compared with saline controls. The pH_i was consistently higher in colonocytes isolated from carcinogen‐injected rats (Prox Cell/AOM = 6.95 vs. Prox Cell/Sal = 6.65, Prox Pect/AOM = 6.75 vs. Prox Pect/Sal = 6.65, Dist Cell/AOM = 6.94 vs. Dist Cell/AOM = 6.85, Dist Pect/AOM = 6.92 vs. Dist Pect/Sal = 6.79) than in cells from saline‐injected rats. Furthermore, in the majority of rats, pH_i was lower in the proximal than in the distal colon. Addition of butyrate to cultured colonocytes consistently lowered pH_i, but the effect was more pronounced in the carcinogen‐injected animals. These data identify changes that occur intraluminally and intracellularly in colons of rats injected with AOM and suggest that, during tumorigenesis, alterations in butyrate production and basic colonocyte physiology may play an important role in the process.     

血清细胞因子水平与新生儿缺氧缺血性脑病病情程度关系

目的新生儿缺血缺氧性脑病(hypoxie-ischemic encephalopathy,HIE)是各种原因引起的脑组织缺血缺氧导致的脑部病变。本研究拟探讨血清细胞因子监测在新生儿缺氧缺血性脑损伤病情严重程度评估中的应用。方法选取2016-01-12-2018-01-30郑州市妇幼保健院新生儿科收治的缺氧缺血性脑损伤患儿106例作为病例组,另选取同期分娩的健康新生儿85名作为对照组。于出生后1d内及出生后第7天采用酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)对所有新生儿血清白细胞介素6(interleukin-6,IL-6)、IL-8、IL-10、IL-1β及肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)水平进行检测,采用方差分析及配对t检验进行组内数据对比。结果急性期患儿TNF-α、IL-10、IL-6、IL-8和IL-1β水平均高于恢复期,差异有统计学意义(均P0.001)。病例组急性期TNF-α、IL-10、IL-6、IL-8和IL-1β水平均低于对照组,差异有统计学意义(均P0.05),各项指标水平均以对照组最低,而且随病情程度加重,各项指标水平逐渐升高。恢复期虽然病例组各项指标均有所降低,但组间比较差异仍然具有统计学意义(均P0.05),变化规律与急性期相似。结论新生儿缺氧缺血性脑病患儿血清细胞因子水平升高,且细胞因子水平与病情严重程度有关。     

血清25-羟维生素D水平与2型糖尿病的关系

目的 研究血清维生素D水平在2型糖尿病发病过程中的作用。方法采用随机分层抽样,共589例志愿者纳入本研究。根据空腹血糖及口服葡萄糖耐量试验结果将志愿者区分为糖尿病人群及非糖尿病人群。共计249例检测了血清25-羟维生素D水平,应用二元Logistic回归分析糖尿病与相关因素的关系,应用Cox—Staurt趋势检验分析各年龄段血清25-羟维生素D水平,按血清25（OH）D四分位值分层,分别计算糖尿病患病优势比OR值及95％置信区间。结果所有年龄段均存在不同程度的25-羟维生素D缺乏,调节年龄、HOMA．IR、BMI后,血清25．羟维生素D水平与糖尿病发病呈剂量依赖的负相关（r=-0．9271,P〈0．01）,当血清25-羟维生素D水平达到94．6nmol／L以上时,糖尿病发病显著降低[OR=0．52,95％CI（0．23—0．78）,P〈0．01]。结论血清25-羟维生素D水平与2型糖尿病发病呈明显负相关,血清25-羟维生素D水平降低增加糖尿病发病风险。     

HBV-DNA与乙型肝炎病毒宫内感染之间的关系

目的 探讨HBV-DNA在乙型肝炎病毒(HBV)垂直传播中的作用.方法 选择2009年1月-2010年1月在我院正规产检的HBsAg阳性孕妇,定量检测其血清HBV-DNA和新生儿脐带血HBV-DNA含量.结果 HBsAg阳性孕妇外周血和新生儿脐带血HBV-DNA含量具有相关性(r=0.401,P<0.001),HBeAg阳性孕妇其新生儿宫内感染率较HBeAg阴性孕妇的新生儿宫内感染率明显升高,差异有统计学意义(P<0.01);脐带血HBV-DNA阳性孕妇中,剖宫产组与阴道分娩组HBV-DNA滴度对数之间差异无统计学意义(t=0.466,P=0.642).结论 母体血清HBV-DNA高滴度和HBeAg阳性是导致宫内感染的高危因素,而且HBV垂直传播与分娩方式无关.     

婴儿微青春期维生素D水平与性腺功能的关系

目的探讨健康婴儿微青春期维生素D水平与性腺激素的关系。方法共100例婴儿纳入研究,其中男婴54例,女婴46例,在出生后30 d^45 d测定维生素D、LH、FSH、TT、E2、AMH和inhibin B水平。将所有婴儿分为维生素D缺乏(<10 ng/ml)、维生素D不足(10 ng/ml^20 ng/ml)和维生素D充足(>20 ng/ml)3组。分析维生素D水平与生殖激素的相关性。结果维生素缺乏组TT水平高于维生素充足组,差异有统计学意义[均值(0.51±0.31)ng/ml VS(0.27±0.22)ng/ml,P<0.05];而维生素D缺乏组的inhibin B则低于维生素充足组[均值:(21.1±15.62)pg/ml VS(53.36±39.36)pg/ml,P<0.05]。结论随机对照试验显示维生素D在微青春期对性腺激素的重要性。维生素D对微青春期女婴的TT和inhibin B有一定的作用,维生素D可能在生命早期对性腺功能有影响。     

Differing effects of pectin, cellulose and lignin on stool pH, transit time and weight

Randomized cross-over studies were undertaken to determine the effects of daily dietary supplements of pectin (12 g/d), cellulose (15 g/d) and lignin (12 g/d) on stool characteristics of healthy volunteers. Detailed dietary records were kept throughout the study. Stool collections over 48 h were used to determine mean stool pH and weight. The single stool transit time was measured using radio-opaque markers. Pectin did not significantly alter the mean stool pH, transit time or 24 h wet weight. Cellulose lowered the mean stool pH from 6.38 to 6.12, decreased mean stool transit time by 27% and increased mean wet stool weight by 57%. Lignin lowered the mean pH from 6.34 to 6.25, decreased the stool transit time by 20% and increased stool weight by 27% but these changes were not statistically significant. These findings have shown that individual fibre components have different colonic metabolic effects and support the view that associations between dietary fibre intakes and diseases such as colorectal cancer should be evaluated with regard to the type of fibre components consumed.     

Relationship between plasma adiponectin levels and metabolic risk profiles in Taiwanese children

OBJECTIVE: Adiponectin, a novel adipokine with antiinflammatory and insulin-sensitizing properties, has an important role in glucose metabolism and is negatively correlated with body fat amount in adults. The purpose of this study was to evaluate the association of plasma adiponectin level with metabolic risk profiles and insulin resistance status among Taiwanese children. RESEARCH METHODS AND PROCEDURES: We enrolled 1,248 children (608 boys and 640 girls) to ascertain their demographic, anthropometric, and cardiovascular risk factors distribution in Taipei. We measured plasma insulin, adiponectin, and leptin levels by radioimmunoassay (Linco Research Inc, St. Charles, MO). We calculated an insulin resistance index (IRI) using the Homeostasis Model Assessment model and also calculated an insulin resistance syndrome (IRS) summary score for each individual by adding the quartile ranks from the distribution of systolic blood pressure, serum triglyceride, high-density lipoprotein-cholesterol (HDL-C) (inverse), and insulin levels. RESULTS: In general, the boys had larger BMI, higher systolic blood pressure, serum total cholesterol, and triglyceride, and lower plasma leptin and adiponectin levels than girls. Plasma adiponectin levels were correlated negatively with BMI, leptin, insulin, IRI, and IRS summary score but positively correlated with HDL-C in both boys and girls. In multivariate regression analyses, adiponectin was negatively associated with insulin (girls only), IRI (girls only), and IRS score, and positively associated with HDL-C in both genders even after adjusting for age, BMI, plasma leptin level, and other potential confounders. DISCUSSION: These data suggest that plasma adiponectin levels were negatively associated with metabolic risk profiles that may have played a protective role in the development of insulin resistance among Taiwanese school children.     

Relationship between blood lead levels and renal function in lead battery workers

OBJECTIVES: The aim of this study was to investigate the correlation between blood lead (PbB) levels and renal function indices of blood-urea nitrogen (BUN), serum creatinine (SC) and uric acid (UA) among lead battery workers with exposure to lead. METHODS: A total of 229 workers of both genders from two lead battery factories were recruited in this cross-sectional study. The personal airborne and blood samples were collected on the same day. The airborne lead (PbA) and PbB levels, and individual renal function parameters were measured and statistically analyzed. RESULTS: A positive correlation between PbB levels and individual renal function index of BUN, SC, and UA was found ( P<0.01). The PbB levels and renal function indices showed significant difference between male and female workers. Based on a multiple regression model, an increment of 10 micro g/dl PbB produced an increase of 0.62 mg/dl BUN, after being adjusted for work duration and age, and an increase of 0.085 mg/dl UA, after being adjusted for gender and body weight. Workers with PbB 60 microg/dl showed a positive dose-effect relationship with significant difference in BUN ( P<0.001) and UA ( P<0.05), and the percentage of workers with BUN and UA over the reference value also showed an increasing trend. CONCLUSION: Blood-urea nitrogen and uric acid could be considered as suitable prognostic indicators of renal dysfunction in lead-exposed workers. Our results showed that PbB levels higher than 60 micro g/dl had increasing chances of inducing adverse renal effects.     

Relationship between ambient sulfur dioxide levels and neonatal mortality near the Mt. Sakurajima volcano in Japan

We examined the association between neonatal mortality and ambient sulfur dioxide (SO2) levels in the neighborhood of Mt. Sakurajima, Yamashita public health district of Kagoshima City, during the period between 1978 and 1988. The analysis using Poisson regression models showed that the monthly average level of SO2 was positively associated with the neonatal mortality (P = 0.002). When the SO2 levels were categorized into four groups to estimate the relative risk (RR) of neonatal mortality using the lowest exposure category as a reference, the RR increased with elevated exposure levels (P for trend < 0.001) and was the highest in the group with the highest level of exposure (RR = 2.2, 95% confidence interval; 1.2-4.1). Other than SO2, we also examined the number of eruptions, the amount of ashfall, and the average level of suspended particulate matter. None of these factors was associated with neonatal mortality. Although the present study suggests that increase in SO2 levels has had an adverse effect on neonatal mortality in the neighborhood of Mt. Sakurajima, it is difficult to determine the source of the SO2. Further studies are necessary to elucidate the mechanisms of the excess neonatal mortality probably associated with the volcanic SO2 levels.     

感染性休克患者D-二聚体水平的变化与弥散性血管内凝血的关系

目的 研究感染性休克患者D-二聚体水平的变化与弥散性血管内凝血（DIC）的关系.方法 将188例感染性休克患者按是否发生DIC分为DIC组（36例）和非DIC组（152例）,患者血清D-二聚体水平采用全自动乳胶微粒增强定量免疫比浊法进行测定,采用受试者工作特征（ROC）曲线计算血清D-二聚体水平的截断值.结果 DIC组和非DIC组休克后1d血清D-二聚体水平比较差异无统计学意义[（1258.69±296.34） mg/L比（1108.45±226.23） mg/L,P＞0.05];DIC组休克后3、5、7d血清D-二聚体水平显著高于非DIC组[（1468.95±448.78） mg/L比（967.76±214.32） mg/L、（1702.12±578.40） mg/L比（886.57±187.22） mg/L、（1924.21±796.76） mg/L比（821.46±178.32） mg/L],差异有统计学意义（P＜0.05）.采用ROC曲线计算血清D-二聚体水平的截断值为303 mg/L,以血清D-二聚体水平≥1203μg/L来判断感染性休克后发生DIC的敏感度为81.5％,特异度为76.3％.结论 感染性休克患者血清D-二聚体水平的变化不仅与患者的疾病严重程度相关,还与患者是否发生DIC有密切的关系.因此测定患者血清D-二聚体水平对感染性休克患者的诊断和判断预后有重要的临床意义.