Rapid In Vitro Plant Regeneration of Black Gram (Vigna mungo L. Hepper) Var. Sarala,an Important Legume Crop

Efficient in vitro regeneration of black gram (Vigna mungo L. Hepper) var. Sarala was achieved through organogenesis using cotyledonary explants excised from 4 days old seedlings on MS medium supplemented with 2.0 mg/l BAP. Organogenic calli were developed from cotyledonary tissues within 4–6 weeks of culture on MS medium supplemented with 3.0 mg/l 6-benzylaminopurine (BAP) along with 2.0 mg/l 1-napthaleneacetic acid (NAA). Shoot bud regeneration was achieved on MS medium supplemented with 2.0 mg/l BAP within 3–4 weeks of subculture. The number of shoots per culture varied from 1.12 to 8.75 in different growth media. The cultures incubated initially on dark photoperiod for 2 weeks and subsequently transferred to 16 h photoperiod showed higher number of shoot bud regeneration. The proliferated shoots were further sub-cultured on similar medium for higher rate of shoot bud regeneration. The elongated shoots were rooted on ½ strength MS medium fortified with 0.1–0.5 mg/l NAA or indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA) with 2 % (w/v) sucrose within 2–3 weeks of culture. The higher percentage of rooting was obtained on 0.1–0.25 mg/l NAA as compared with IBA or IAA. The rooted plantlets were transferred to soil mixture (soil: sand: vermi-compost, 1:1:1 ratio) and kept in the greenhouse with 85 % humidity. The regenerated plantlets were successfully grown with 75 % survival rate. This protocol can be used for genetic improvement of black gram.     

Microbial Consortium Improved Growth and Performance of Teak (Tectona grandis L.f.) in Nursery and Field Trials

Proceedings of the National Academy of Sciences, India Section B: Biological Sciences - The response of teak (Tectona grandis L.f.) to the selected microbial consortium (Ambispora...     

Bacillus sonorensis,a Novel Plant Growth Promoting Rhizobacterium in Improving Growth,Nutrition and Yield of Chilly (Capsicum annuum L.)

Proceedings of the National Academy of Sciences, India Section B: Biological Sciences - Plant growth promoting rhizomicroorganisms (PGPR) play an important role in improving plant growth, nutrition...     

Effect of Boron on the Contents of Chlorophyll,Carotenoid, Phenol and Soluble Leaf Protein in Mung Bean,Vigna radiata (L.) Wilczek

Greenhouse experiments were carried out to study the effect of boron (B) on certain biochemical constituents considering mung bean, Vigna radiata (L.) Wilczek as an experimental crop. B as boric acid (H₃BO₃) was applied at the concentrations of 0, 1, 2, 4, 8, 16 and 32 μg B g^?1 soil. Lower applied doses of B up to 4 μg g^?1 resulted in an increase in chlorophyll and carotenoid contents and decrease in stress indicators viz. total phenol content and soluble leaf protein content. Maximum increment in chlorophyll and carotenoid contents was observed at 4 μg g^?1 B concentrations, while at this concentration (4 μg g^?1) maximum decrease in total phenol content and soluble leaf protein content was observed. Reciprocal relationship between total chlorophyll contents and total phenol content was observed. Adverse effect on selected biochemical constituents beyond 4 μg g^?1 B concentrations reflected the importance of efficient and appropriate use of boron in agronomic practices for mung bean.     

Improvement of Growth,Photosynthesis and Antioxidant Defense in Rice (Oryza sativa L.) Grown in Fly Ash-Amended Soil

Proceedings of the National Academy of Sciences, India Section B: Biological Sciences - Due to the hostile conditions created by fly ash, its utilization in vegetation is restricted. Therefore, fly...     

Development of Microbial Consortia for Growth Attributes and Protein Content in Micropropagated <Emphasis Type="Italic">Bacopa monnieri</Emphasis> (L.)

The study was conducted for evaluation of the effect of microbial consortia for growth attributes and protein content of micropropagated plants of Bacopa monnieri. Five microbial strains e.g., Pseudomonas sp. BHUPSB04, Pseudomonas sp. BHUPSB06, Burkholderia sp. BHUPSB03, Bacillus sp. BHUPSB13 and Paenibacillus sp. BHUPSB16 were isolated and characterised on the basis of biochemical and molecular characters. Burkholderia sp. BHUPSB03 was screened as an effective and significant for phosphate solubilisation, production of indole-3-acetic acid, ammonia, hydrogen cyanide and siderophores. Hydrogen cyanide was produced by other strains namely Bacillus sp. BHUPSB13, Paenibacillus sp. BHUPSB16 and Pseudomonas sp. BHUPSB04. Higher indole-3-acetic acid production was recorded with Burkholderia sp. BHUPSB03 followed by Bacillus sp. BHUPSB13, Pseudomonas sp. BHUPSB04 and Pseudomonas sp. BHUPSB06 as compared to A. Brasilense and T. harzianum. Tetra inoculation of microbial consortia Azospirillum + Pseudomonas sp. BHUPSB06 + Trichoderma + Burkholderia sp. BHUPSB03 showed maximum growth attributes like shoot length, number of branching, dry weight and protein content in micropropagated plants of B. monnieri followed by tri-inoculation of A. brasilense + Pseudomonas sp. BHUPSB06 with Burkholderia sp. BHUPSB03/Pseudomonas sp. BHUPSB04/Paenibacillus sp. BHUPSB16/Bacillus sp. BHUPSB13 as compared to control and other treatments. Therefore, tetra and tri inoculations may be used as most efficient microbial consortia for enhancing the growth attributes and protein content in shoots of B. monnieri.