涎腺黏膜相关淋巴组织型淋巴瘤报告1例及文献系统分析

涎腺黏膜相关淋巴组织型淋巴瘤（Salivary glands mucosa-associated lymphoid tissue lymphoma ,SGML）,为无痛性,进展缓慢的恶性肿瘤,较少发生于头颈部。口腔颌面外科医生因认识不足,临床上易误诊。本文报告以1例典型多发性涎腺黏膜相关组织型淋巴瘤为案例,结合文献分析探讨涎腺黏膜相关淋巴组织型淋巴瘤的发病机制及诊疗方法。     

涎腺非何杰金氏淋巴瘤

发生于涎腺的淋巴瘤多为非何杰金氏淋巴瘤,发病率低,临床、病理诊断较为困难。本文就发生于涎腺的非何杰金氏淋巴瘤的发病情况、临床、病理、免疫组化特征、与EB病毒的关系、鉴别诊断、预后及近年来发现的结外淋巴瘤的新类型——涎腺粘膜相关淋巴组织淋巴瘤作一综述,以供临床、病理医生参考。     

涎腺非何杰金氏淋巴瘤

发生于涎腺的淋巴瘤多为非何杰金氏淋巴瘤，发病率低，临床，病理诊断较为困难，本文就发生于涎腺的非何杰金氏淋巴瘤的发病情况，临床，病理，免疫组化特征，与ＥＢ病毒的关系，鉴别诊断，预后及近年来发现的结外淋巴瘤的新类型－涎腺粘膜相关淋巴组织淋巴瘤作一综述，以供临床，病理医生参考。     

恶性淋巴上皮病2例

恶性淋巴上皮病２例谭清和，胡北平恶性淋巴上皮病变是发生于涎腺的少见恶性肿瘤，可以为原发性，也可以由良性淋巴上皮病变演变而来。特别是良性淋巴上皮病中的一种类肿瘤型假性淋巴瘤，其可能性更大。因为良性淋巴上皮病变中的淋巴成份可恶变为涎腺内或涎腺外的恶性淋巴...     

颊部小涎腺淋巴上皮病恶性淋巴瘤变1例

颊部小涎腺淋巴上皮病恶性淋巴瘤变１例刘渊，姜晓钟，刘会敏淋巴上皮病在口腔科临床并不罕见，一般为良性过程，病程可能很长而无特殊危害，但淋巴上皮病的上皮和淋巴成份可恶变为癌和恶性淋巴瘤。本科收治１例颊部小涎腺淋巴上皮病，发病１７年后恶性淋巴瘤变，现报告如...     

良性淋巴上皮病恶变为淋巴瘤2例报告

良性淋巴上皮病变中淋巴成份恶变即为涎腺内或涎腺外的恶性淋巴瘤，而上皮成份可恶变为恶性淋巴上皮病。类肿瘤型良性淋巴上皮病如发生恶变，其间隔时间可长达２０年，平均为０５～２９年，其发生率国外资料为０１６％，国内报道为０３１％。近期本院涎腺专科门诊有二例确诊为良性淋巴上皮病患者最后演变为恶性淋巴瘤。现报告如下。１　病例介绍　　病例１：女性，７１岁，５年前发现口咽鼻眼干燥，日渐加重，同时右腮腺区逐渐弥漫性肿大。３月前偶然发现右侧耳下一蚕豆大小肿块，唇腺活检示良性淋巴上皮病。给予ＳＳ糖浆、氯喹、地塞…     

涎腺对称性肿块的MRI诊断特征

目的:通过研究总结双侧涎腺肿块的临床表现及其MRI征像特征,并与病理学检查结果相对照,探讨双侧涎腺肿块的临床鉴别要点,以提高其确诊率。方法:收集双侧涎腺对称性肿块患者21例,回顾性分析其临床资料、MRI征象及病理学诊断,评价临床表现结合MRI检查对诊断的意义,并从中归纳出具有鉴别诊断意义的MRI特征。结果:本组21例双侧涎腺肿块,经病理学诊断,分别为淋巴上皮病变5例,涎腺慢性炎症7例,涎腺良性肥大3例,腺淋巴瘤2例,嗜酸性淋巴肉芽肿2例,恶性淋巴瘤及涎腺脂肪增生各1例;临床表现结合MRI检查得到准确诊断的共16例,总诊断符合率为76.2%,其中对于淋巴上皮病变的诊断符合率为95.2%,对于涎腺慢性炎症的诊断符合率为90.5%。结论:双侧涎腺对称性肿块以淋巴上皮病变或涎腺慢性炎症为多见,在MRI影像学上具有其特征性表现。     

涎腺粘膜相关组织型淋巴瘤2例报道及文献复习

粘膜相关淋巴组织(MALT)型淋巴瘤80年代初由Issacson和Wright首先报道[1],因其具有不同于其它类型淋巴瘤的临床病理特点,近年来成为淋巴瘤的研究热点,但国内原发于涎腺的MALT淋巴瘤的报道尚不多见,为了更好的认识此病,我们对本科近年来遇到的涎腺恶淋及其它淋巴组织增生性疾病进行回顾性观察,现将其中2例通过高年资病理医生确诊,并经免疫组化证实的MALT淋巴瘤病例报道如下,结合文献复习对其临床病理特征,诊断与鉴别诊断及发病机理进行讨论.     

涎腺粘膜相关淋巴组织淋巴瘤

涎腺粘膜相关淋巴组织淋巴瘤是近年逐渐被认识的一类B细胞来源的非何杰金氏淋巴瘤。多发于50岁以上的女性,与干燥综合征关系密切,病变多为无痛性过程,发展缓慢,长期局限在涎腺原发灶,其他结外器官极少受累。病理学、细胞学及遗传学方面有特异表现。诊断依靠病理检查,免疫组化、细胞学及遗传学检查有辅助作用。预后较好。     

EB病毒感染与涎腺良性淋巴上皮病损恶变的关系

目的 了解Epstein-Barr病毒(EBV)感染与涎腺良性淋巴上皮病损恶变的关系。方法 采用针对EB病毒编码的小分子RNA(EBER-1)的寡核苷酸探针对2例涎腺良性淋巴上皮病损灶性恶变、14例恶性淋巴上皮病损(MLEL)、4例良性淋巴上皮病损(BLEL)、4例非特异性慢性涎腺炎石蜡包埋组织进行原位杂交检测;采用聚合酶链反应(PCR)进行EBV BamHI-W片段检测。结果 2例良性淋巴上皮病损灶性恶变形态学上良性区域或恶性区域,EBER-1及EBV BamHI-W片段均为阳性,14例MLEL的癌细胞核均可见EBER-1强阳性信号,其中12例EBV BamHI-W片段阳性,4例BLEL及4例非特异性慢性涎腺炎均为阴性。结论 少数恶性淋巴上皮病损可能在BLEL基础上发生,EB病毒感染与涎腺良性淋巴上皮病损癌变发生可能有密切关系,EBV感染可发生在BLEL癌变形态学改变之前。     

柠檬精油对牙周致病菌抑制作用及其安全性初步研究

目的:探讨柠檬精油对牙周致病菌的体外抗菌活性及对细胞增殖的影响。方法:采用微量液体稀释法测定柠檬精油对Pg、Fn、Aa、Pi的最小抑菌浓度(minimal inhibitory concentration,MIC)及最小杀菌浓度(minimum bactericidal concentration,MBC);以较低浓度的MIC为标准稀释LEO作为实验组,采用MTT法测定柠檬精油对HUVECs的毒性作用,明确抑菌浓度下LEO的安全性。结果:柠檬精油对牙周主要致病菌均有抑菌作用,Pg、Fn、Aa、Pi的MIC分别是9.0 g/L、4.5 g/L、4.5 g/L、9.0 g/L,Aa、Fn的 MBC是9.0 g/L,Pg、Pi的MBC未测得。1/2MIC、1/20MIC浓度的LEO能够抑制人脐静脉内皮细胞的生长,而低于1/200MIC浓度的LEO则对人脐静脉内皮细胞的生长没有影响,其中1/200MIC浓度的LEO作用明显优于0.02%的CHX。结论:体外环境中,柠檬精油对牙周致病菌Pg、Fn、Aa、Pi具有抗菌活性,低浓度应用对机体相对安全。     

Oral cancer: molecular technologies for risk assessment and diagnosis

Purpose: The effective biomarkers related to diagnosis, metastasis, drug resistance and irradiation sensitivity of oral cancers will help the pathologist and oncologist to determine the molecular taxonomy diagnosis and design the individualization treatment for the patients with oral cancers.     

Computer-assisted planning for maxillofacial prosthodontic rehabilitation

Computerized tomography (CT) planning and the use of CT derived surgical templates for implant placement have shown promise for restoring function within months after surgical reconstruction of acquired post-oncologic defects.     

Stimulated whole saliva components in children with Down syndrome

The authors report on the components of stimulated whole saliva from children with Down syndrome—including pH, flow rate, sialic acid and protein concentrations, and amylase and peroxidase activity. Saliva samples were collected from 35 children aged 6–10 years. Of the participants, 17 had Down syndrome and 18 did not. To stimulate saliva production, the children chewed a piece of parafilm for 10 minutes before the sample was collected. Soon after collecting the saliva sample, the authors measured pH using a portable pH-meter. Sialic acid levels were determined with a thiobarbituric acid assay. Protein content was determined with Folin's phenol reagent. Amylase was assayed and the authors measured the maltose produced by the breakdown of starch and peroxidase using ortho-dianisidine.
No statistically significant difference was observed in levels of sialic acid (free and total) between the two groups. Protein concentration was about 36% higher in the group with Down syndrome. However, the salivary flow rate, pH, and amylase and peroxidase activities were lower among the children with Down syndrome.     

Effect of Pingyangmycin on human venous malformation endothelial cells

Purpose: Venous malformations are common vascular anomalies with a propensity of the head and neck. Intralesional injection of Pingyangmycin (PYM, bleomycin A5 hydrochloride) is a widely used sclerotherapy method for the treatment of venous malformation.     

牙髓再生的研究现状

目前,根管治疗术在临床上已得到了广泛的应用并成功的保存了患牙,但其仍有许多术后并发症。随着组织工程技术在口腔领域的应用,构建一种组织工程化牙髓进行牙髓-牙本质复合体的再生治疗成为可能。近年来国内外已有很多关于牙髓再生的研究,并且已经取得了一定成果。本文将对目前国内外牙髓再生的研究进展现状作一综述。     

替牙期骨性Ⅲ类错牙合患者颞下颌关节特征的锥形束CT研究

目的:采用锥形束CT(CBCT)研究替牙期骨性Ⅲ类错牙合患者颞下颌关节的影像学特征,探讨Ⅲ类功能状态下,颞下颌关节的生长、改建机制。方法:从就诊于昆明医科大学附属口腔医院正畸科的患者中选取符合纳入标准的替牙期骨性Ⅲ类错牙合患者及骨性Ⅰ类错牙合患者各20名,使用NNT viewer 5.3图像处理软件进行三维重建及线距和角度的测量,并进行统计学分析。结果:替牙期骨性Ⅲ类错牙合患者组和替牙期骨性Ⅰ类错牙合患者组对比结果为:矢状面双侧关节前间隙偏小、双侧关节上间隙偏小、双侧关节结节斜度偏小;冠状面双侧关节内间隙偏小,双侧关节上间隙偏小,双侧关节外间隙偏小,右侧髁状突角度偏小;横截面右侧髁状突前后径偏小。结论:替牙期骨性Ⅲ类错牙合患者颞下颌关节发育不充分,呈现髁状突,关节窝深度,关节结节斜度;冠状面关节内、外间隙均较小的特征。骨性Ⅲ类错牙合患者髁状突在关节窝中处于前置近关节窝顶位置。骨性Ⅲ类错牙合患者颞下颌关节影像学特征与其功能状态相适应。     

Ghlorhexidine as a root canal medicament for treating inflammatory lesions in the periodontal space

Abstract— The purpose of the present study was to investigate the therapeutic effect of intra-canal application of chlorhexidine on inflammatory root resorption. Dental pulps from monkey incisors were infected and resealed prior to extraction of the teeth. Root dentin was mechanically exposed and the teeth were replanted under aseptic conditions. After 4 weeks, the experimental teeth were dressed with chlorhexidine gel and resealed. Animals were sacrificed 4 weeks later, and their jaws prepared for histologic examination. Results showed that both marginal and apical periodontal inflammation and resorption were reduced in the chlorhexidine-treated teeth. It was concluded that the use of intra-canal chlorhexidine may be a useful adjunct in the treatment of inflammatory root resorption, but further human trials need to be undertaken before its clinical use can be recommended.     

小鼠牙髓干细胞同种异体移植后体内免疫反应初探

目的:初步探究小鼠牙髓干细胞同种异体移植后的全身免疫反应。方法:牙髓干细胞分离获得自C57BL/6 小鼠牙髓组织,培养至第3 代,与纳米羟基磷灰石(n-HA)复合培养形成复合物,扫描电镜观察其生长黏附情况。40只4周龄的C57BL/6 小鼠随机分成两组。实验组将复合物移植入上背部皮下,对照组将纳米羟基磷灰石植入上背部皮下,术后均使用外科方法严密缝合创口。两组动物术后均观察4周、6周处死,分离脾脏,制成脾淋巴细胞悬液,流式细胞仪测定CD4⁺、CD8⁺的值。结果:牙髓干细胞在体外与纳米羟基磷灰石结合良好;免疫组化染色实验组4周、6周均可见牙本质涎蛋白(dentin sialoprotein,DSP)阳性表达;实验组与对照组相比,术后4周、6周CD4⁺/CD8⁺值均无明显差异。结论:牙髓干细胞同种异体移植后,尚不能够引起机体的免疫排斥反应,其在体内仍具有低免疫原性,有望成为组织工程同种异体种子细胞来源。