舍格林综合征患者T细胞亚群,Il—2活性及Ts细胞功能的研究

本文应用单克隆抗体OKT系列、IL—2活性检测及短寿命Ts功能测定技术,对20例舍格林综合征(Sjog ren's syndrome,SS)患者末稍血T淋巴细胞亚群、PBL产生IL—2能力及抑制性T细胞的功能进行了研究。结果显示SS在发病期间OKT_3、OKT_4阳性细胞比率较正常对照组明显降低(P<0.001),OKT_3阳性细胞比率高于正常对照组(P<0.001)。PBL产生IL—2较正常对照组明显降低(P<0.001),但Ts功能和正常对照组无明显差异(P>0.05)。原发性SS(6例)和继发性SS(14例)PBL产生IL—2活性及Ts功能,两组SS之间无明显差异。结果提示T_H细胞数量及功能降低,T细胞亚群数量及功能上失衡与本病的发病相关。     

舍古林综合征中药治疗前后T细胞亚群的变化

本文对３８例舍古林综合征患者进行了Ｔ细胞亚群的检测。发现该病患者有Ｔ８减少，Ｔ４／Ｔ８增高的倾向。用“活血生津丸”治疗后，Ｔ９水平及Ｔ４／Ｔ８比例恢复正常。推论该病的自身免疫状态与Ｔ４／Ｔ８比例失衡有关。中药的治疗作用在于调整了这种失衡的状态。     

中西医结合治疗舍格林氏综合征

对按照口腔干燥，干燥性角结膜炎和类风湿性关节炎三大症状中两项存立而确诊［１］的１２例舍格林氏综合征（以下简称Ｓ．Ｓ）患者进行了临床及血液、血清学检查并给予中西医结合的方法治疗，现将检查结果及疗效报告如下：临床资料１一般情况：观察对象１２例，均为１９８...     

Sjoegren综合征中T细胞亚群值的测定

目的：研究Ｓｊｏｅｇｒｅｎ综合征中Ｔ细胞亚群和免疫球蛋白的变化,方法：应用单克隆抗体技术检测Ｔ细胞亚群,用单向免疫扩散法检测ＩｇＡ,ＩｇＧ,ＩｇＭ。结果：Ｓｊｏｅｇｒｅｎ综合征患者外周血中ＣＤ＾４降低（Ｐ〈０．０５）,ＣＤ＾８升高（Ｐ〈０．０５）,其程度与病情发展相一致,严重者ＣＤ＾４／ＣＤ＾８倒置,ＩｇＡ,ＩｇＧ,ＩｇＭ均升高（Ｐ〈０．０５）,尤以ＩｇＧ显著（Ｐ〈０．０１）,结论：Ｓｊｏｅｇｒｅ     

Sjogren综合征患者外周血T细胞亚群测定

本文采用间接免疫萤光法，用抗人Ｔ细胞亚群单克隆抗体，测定１２例ＳＳ患者外周血Ｔ淋巴细胞亚群，结果发现，与正常对照者相比，ＣＤ３降低，ＣＤ４升高，ＣＤ８升高，ＣＤ４／ＣＤ８降低，经过统计学处理，ＣＤ３两组间有显著差别（Ｐ〈０．０５），ＣＤ４两组间无显著差别（Ｐ〉０．０５），ＣＤ８两组有非常显著差别（Ｐ〈０．０１），ＣＤ４／ＣＤ８两组间有非常显著差别。     

舍格林综合征唇腺组织中CD45的表达及意义

目的：研究舍格林综合征(Sjogren’s Syndrome，简称SS)患者唇腺组织中过度浸润的淋巴细胞上的白细胞共同分化抗原(Leukocyte common antigen-45，CD45)的表达，以探讨淋巴细胞在其免疫反应中的作用。方法：通过HE染色观察淋巴细胞的浸润特点，采用免疫组织化学法检测CD45R、CD45RO在淋巴细胞上的表达。结果：唇腺组织过度浸润的淋巴细胞中CD45RO阳性表达的T细胞占65．74％，CD45R阳性表达的B细胞占27．38％．结论：在SS病变中是以细胞免疫为主、体液免疫为辅的免疫反应。     

HLA-DR在舍格林综合征病变中的作用

目的 探讨舍格林综合征（Sjoegren′s Syndrome，简称SS）患者唇腺组织中的淋巴细胞过度浸润与人类主要组织相容性系统Ⅱ类抗原（HLA－DR）的关系。方法 通过HE染色观察淋巴细胞的浸润，采用免疫组织化学法检测HLA－DR的表达，运用图象分析技术对结果进行定量分析。结果 唇腺组织中的HLA－DR表达与淋巴细胞侵润密切相关；淋巴细胞浸润密集的组织，炎症浸润广泛，导管上皮增生与腺泡破坏显著。结论 在SS的发生、发展中HLA－DR与淋巴细胞浸润呈正相关。     

舍格林氏综合征免疫病理特性、病因探讨及诊断

舍格林氏综合征(SS)是一种以外分泌隙损伤为主的常见全身性自身免疫性疾病。其特异性免疫学变化包括血中出现涎腺导管上皮抗原抗体;局部腺体组织浸润淋巴细胞中T辅助细胞增高、B细胞功能亢进。病因研究发现EBV,HIV,HCV,HTLV-Ⅰ等病毒与SS发生可能相关。SS的诊断需要临床、免疫学及病理学三方面指标的结合,目前尚缺乏全球公认的统一诊断标准。     

着色性干皮病伴舍格林综合征及基底细胞癌1例

着色性干皮病 (ＸｅｒｏｄｅｒｍａＰｉｇｍｅｎｔｏｓｕｍ ,ＸＰ)是一种罕见的常染色体隐性遗传病 ,癌变率高 ,常表现在口腔颌面部。鉴于本病罕见 ,且并发腮腺舍格林综合征恶变 ,国内尚无报道。现将我院收治 1例报告如下 :病例报告患者 ,男 ,30岁。 2 0年前出现面颈部广泛雀斑样色素沉着 ,不痛不痒。 15年前眶下色素沉着区开始溃烂 ,涂药后可愈合 ;反复发作 ,逐渐形成疤痕 ,下睑外翻。 5年前右眶下区疤痕溃烂 ,多次用药 ,至今不愈。来我院皮肤科就诊 ,溃烂处活检病理报告 :符合基底细胞癌 ;临床诊断为 :①右眶下区基底细胞癌②着色性干皮病…     

干燥综合征涎腺组织细胞凋亡和Fas抗原表达的研究

为进一步探讨干燥综合征(Sjogren Syndrome,SS)涎腺损害的机理,通过对18例SS患者组和6例对照组的研究,从形态上观察到:SS组淋巴细胞浸润在3级以上的病例(n=10),其部分导管上皮和腺泡细胞有典型的细胞凋亡征象,用DNA末端标记不仅确认为这10例中细胞凋亡的存在,而且发现了另外6例也有细胞凋亡,结果与对照组有显著性差异(P<0.01)。Fas抗原检测结果:18例患者均有不同程度表达,对照组未见阳性表达。表明涎腺组织细胞凋亡可能是SS口腔病损的主要形式之一,而且与Fas抗原表达及介导有关。淋巴细胞浸润严重组(n=10)与轻微组(n=8)之间Fas抗原表达有显著性差异(P<0.01)。表明Fas表达及介导的腺体组织的细胞凋亡与淋巴细胞浸润有关。     

Dimensional change of refractory materials used for ceramic veneers

The current literature considers a number of clinical factors which affect the fit of all-ceramic laminate veneers. However, little consideration has been given to the refractory die materials, and the laboratory techniques used during the construction of these restorations. This study found a wide range of dimensional change occurred during setting and through six firing cycles, for seven refractories recommended for the construction of laminate veneers. It is therefore important that where patient treatment involves the use of veneers the clinician considers the suitability of the materials offered by the laboratory, in order to obtain the optimum marginal integrity.     

Immune reconstitution inflammatory syndrome in HIV infection: taking the bad with the good

In this review, we will describe the immunopathogies of immune reconstitution inflammatory syndrome, IRIS. IRIS occurs in a small subset of HIV patient, initiating combination antiretroviral therapy (ART), where immune reconstitution becomes dysregulated, resulting in an overly robust antigen‐specific inflammatory reaction. We will discuss IRIS in terms of the associated coinfections: mycobacteria, cryptococci, and viruses.     

Immune competent cells in non-irradiated autologous jejunal grafts used for reconstruction of the oral cavity and oropharynx

Autologous jejunal grafts used for primary reconstruction in cases of extensive soft tissue defects following tumor resection in the upper aerodigestive tract were investigated by immunohistochemistry (APAAP technique). Biopsies from eight patients were taken intra-operatively, at the time of transplantation and 2, 4, and 6 months post-operatively. A panel of monoclonal antibodies directed against surface antigens of the major subpopulations of the immune system was used. In all of the patients, a remarkable increase of all antigens investigated was detected 2 months post-operatively, which remained unchanged in the following biopsies (4 and 6 months postoperatively). Significantly higher numbers of CD45RA+ and CD45RO+ (P<0.05) leukocytes were detectable. This increase was due to both subsets of T and B cells, but only for CD19+ B cells was the increase significant. In addition, NK cells (CD16+ lymphocytic cells, P<0.01) and mature macrophages (25F9+ cells, P<0.01) increased. The first post-operative biopsy showed a significantly higher expression of activation-associated antigens (ICAM-1, VCAM, and HLA-DR) on monocytes/macrophages and endothelial cells. Our findings indicate that autologous jejunal grafts facilitate immunological function in the new microenvironment.     

腭-心-面综合征患者语音治疗方法探讨

目的:探讨腭-心-面综合征异常语音患者有效的语音治疗方法。方法:选取腭-心-面综合征行咽成形术后患者57例(男30例,女27例),年龄4-17岁(平均10.6岁)。在汉语语音教学基础上,用简单易学的行为模式进行诱导,建立正确的发音部位与发音方法。比较治疗前、后的语音清晰度和过度鼻音,采用SPSS16.0软件包分别对数据进行t检验和Wilcoxon符号秩和检验,评价疗效。结果:57例患者经2-8个月的语音治疗,语音清晰度由治疗前的33.75%提高到治疗后的97.86%,差异显著(P<0.01)。治疗前轻度过度鼻音7例,中度33例,重度17例;治疗后异常鼻音消除者38例,轻度异常鼻音17例,中度异常鼻音2例,经秩和检验,两者具有显著差异(P<0.01)。结论:对存在严重语音障碍并伴有学习能力缺陷的腭-心-面综合征患者,在汉语语音教学基础上,应用行为诱导模式,可以恢复正常语音清晰度。     

Roberts syndrome,normal cell division,and normal intelligence

Roberts-SC phocomelia syndrome (RS) is an autosomal recessive disorder of symmetric limb defects, craniofacial abnormalities, pre- and postnatal growth retardation, and mental retardation. Patients with RS have been reported to have premature separation of heterochromatin of many chromosomes and abnormalities in the cell-division cycle. No case has been reported who had normal intelligence and normal cell division with typical clinical features of the RS. We report a case of a six-year-old male of clinical and radiologic findings of typical RS with normal cell division and normal intelligence.Although he showed growth retardation, his intelligence was normal. Van Den Berg and Francke later reported that 79 out of 100 cases of Roberts syndrome had premature cell separation (PCS). We think that this case may demonstrate severe expression of the Roberts syndrome even though PCS is not exhibited. The limb involvement of this case was symmetrical, and he showed phocomelia of upper limbs, equinus valgus deformity of ankle, aplasia of fibula, and shortness of fifth toes while his hands and feet were normal with 5 rays each. Craniofacial abnormalities of this case were typical; he showed scaphocephaly, mild hypertelorism, mandibular hypoplasia, dysplastic helix of ear, narrowing of external auditory canal, and cleft palate with wide gap.This report supports the theory that normal intelligence can make social-personal adjustment possible even if all of the stigmata of Roberts syndrome is present.     

Clear cell change in a calcifying odontogenic cyst

A case of calcifying odontogenic cyst exhibiting clear cell change is described. Histomorphologic similarities to the clear cell type of calcifying epithelial odontogenic tumor were noted and the importance of differential diagnosis from clear cell types of salivary gland neoplasms and metastatic hypernephroma are emphasized.