Study on the Effects of FCu-IUD and FICu-IUD on Matrix metalloproteinases in Human Uterine Flushing and Endometrium

It is well know thatmatrix metalloproteinases(MMPs) participate in tissue remodeling duringphysiological and pathological processes,especiallyendometrial cycling,inflammation,tumor invasionand so on[1— 3 ] .We have observed that the activityand kinds of MMPs in the uterine flushing and en-dometrial tissue of FCu- IUD- bearing rabbits wereincreased significantly,the activity of some kindsof MMPs in the FICu- IUD- bearing rabbits weredecreased significantly as compared with the FCu-IUD…     

不同类型宫内避孕器对子宫内膜微血管影响的形态测量研究

采用ERNST-LEITZ-WETZLAR ASM形态测量系统,测定4种不同类型宫内避孕器(IUDs)对兔子宫内膜微血管总面积(TABV)、微血管面积在内膜中所占百分比(PBV)和微血管密度(DBV)的影响。结果表明,与空白自身对照子宫比较,凡接触IUDs 30d后的兔子宫,内膜中TABV、PBV和DBV均有所增加,TABV与PBV增加程度在不锈钢避孕器组(SS-IUD)和孕激素避孕器组(LNG-IUD)差异有显著性意义(P<0.05).组间比较,TABV与PBV增加程度顺序分别为SS-IUD>LNG-IUD、含孕激素与钢避孕器>含铜避孕器。     

The effect of CuIUD on estradiol receptor in the endometrium of rat — An autoradiographic study

Summary Autoradiography (ARG) and scanning microspectrophotometry were applied to study the changes of eosinophilic type II estradiol-binding sites in the endometrium of Sprague-Dawley rats with regular 4 -day estrous cycle, after 30 days of bearing a copper intrauterine device (CuIUD; 40.8mm²) in uterus.³H™estradiol™l7β (³H™E₂) labelled eosinophils were mainly distributed in the deep endometrial stroma and varied in quantity with the estrous cycle. In comparison with the contralateral uterine horn, the number of³H-E₂ labelled cells was significantly reduced in the CuIUD-bearing horn at estrus and diestrus The mean density of silver grains in the ARG positive eosinophils in the endometria exposed to CuIUD was decreased at diestrus, as compared with that in contralateral horns, and at proesttus with that in contralateral and control-group horns. The results suggested that the decrease of type II estradiol-binding sites induced by CuIUD may be closely related to the contraceptive action of copper.     

释放吲哚美辛的固定式铜宫内节育器减少子宫出血机制的研究

观察放置３种不同类型宫内节育器（ＩＵＤ）妇女置器前、后子宫内膜超微结构及官腔冲洗液前列腺素（ＰＧｓ）含量、组织型纤溶酶原激活剂（ｔ－ＰＡ）和纤溶酶原激活剂抑制剂（ＰＡＩ）活性以及凝血因子Ⅷ相关抗原（ｖＷＦ）含量的动态变化。结果：①ＦＩＣｕ－ＩＵＤ对子宫内膜损伤较ＦＣｕ－ＩＵＤ及ＴＣｕ１２２０Ｃ明显减轻。②置入ＦＣｕ－ＩＵＤ和ＴＣｕ２２０Ｃ后１、３、６个月,６－ｋｅｔｏ－ＰＧＦ_（１ａ）／ＴＸＢ_２比值显著高于置器前（Ｐ＜０．０１或Ｐ＜０．０５）,ＰＧＥ_２／ＰＧＦ_２比值升高不明显;置器后ｔ－ＰＡ活性升高显著（Ｐ＜０．０１）,ＰＡＩ活性则无明显变化;ｖＷＦ含量置器前、后无统计学差异。③放置ＦＩＣｕ－ＩＵＤ前后,以上各项生化指标的差异均无统计学意义,但与同期 ＦＣｕ－ＩＵＤ和 ＴＣｕ２２０Ｃ相比,６－ｋｅｔｏ－ＰＧＦ_（１ａ）／ＴＸＢ_２比值及 ｔ－ＰＡ活性下降有显著性意义。提示：ＦＩＣｕ－ＩＵＤ释放的吲哚美辛通过减轻Ｃｕ－ＩＵＤ引起的内膜损伤和异物反应,降低Ｃｕ－ＩＵＤ所致的ＰＧＩ２、ｔ－ＰＡ升高和提高ＴＸＡ_２水平,是其减少子宫出血的部分机制。     

子宫内膜HOXA10表达水平与吉妮宫内节育器相关性的研究

目的：研究子宫内膜HOXA10表达水平与吉妮宫内节育器的相关性。 方法：从使用吉妮宫内节育器的26名育龄女性（实验组）及10名未使用任何宫内节育器及激素避孕药的育龄女性（对照组）获得的子宫内膜活检标本,通过免疫组化方法进行HOXA10表达分析。结果：实验组的子宫内膜HOXA10的表达为4.39±1.4,弱于对照组的8.88±1.79 (P<0.01)。结论：实验组HOXA10表达显著下降,HOXA10表达的水平是子宫内膜容受性的重要指标,铜质吉妮宫内节育器作用机制可能涉及基因抑制子宫内膜容受性,HOXA10表达下降可能是导致铜质宫内节育器避孕作用的主要原因。     

放置宫内节育器后出血的宫腔镜及子宫内膜组织病理学检查

分析宫腔镜及子宫内膜组织病理学检查在查找置入宫内节育器（ＩＵＤ） 后出血原因中的作用。应用电视宫腔镜对１２０ 例ＩＵＤ后出血病例进行镜检， 并对其中１００ 例行诊刮及子宫内膜组织病理检查。结果： 经宫腔镜发现子宫内膜出血１０５ 例次 （６９．５％ ）， 内膜息肉及息肉样增生２９ 例次 （１９．２％ ）， 子宫粘连１１ 例次 （７．３％ ）， 子宫肌瘤６ 例次（４．０％ ）， ＩＵＤ形态与位置异常２９例次（２４．２％ ）。子宫内膜组织病理学检查１００ 例， 其中增生期及分泌期子宫内膜５２例（５２．０％ ）， 子宫内膜增生过长４４ 例（４４．０％ ）。结论： ＩＵＤ后出血的主要原因为ＩＵＤ对子宫内膜局部的机械性压迫，引起内膜充血、坏死及出血； ＩＵＤ的位置、大小与宫腔不相匹配； 合并子宫或子宫内膜病变如子宫内膜增生过长。宫腔镜结合子宫内膜组织病理检查可较准确地诊断ＩＵＤ后出血的原因。     

阴道三维超声评估宫腔粘连患者的子宫内膜容受性及其对妊娠的影响

目的 探讨经阴道三维超声评估宫腔粘连患者子宫内膜容受性的临床价值及其对妊娠的影响.方法 选取2019年1月—2020年8月华中科技大学协和深圳医院经宫腔镜确诊宫腔粘连的116例女性患者作为观察组,选取同期该院行妇科检查的健康女性60例作为对照组.研究对象在排卵日和行宫腔镜下宫腔粘连分离术后,均进行经阴道三维超声检查.测...     

The mechanical study of vascular endothelial growth factor on the prevention of restenosis after angioplasty

Restenosis following percutaneous transluminalcoronary angioplasty ( PTCA) is one of the majorre-search subjects of coronary heart disease( CHD) .The mechanism is quite complicated and not yetclear,buttheimpairmentofendothelium isknown asthe primary evocative factor of the progress ofrestenosis.It is supposed to prevent the restenosisfollowing PTCA by accelerating restoration of en-dothelial integrity and function.In the experimentalstudies,it was proved that exogenic vascular en-dothelial…     

Effect of buyang huanwu decoction on plasma thromboxane b2, 6-keto-prostaglandin f1α, endothelin and calcitonin gene related peptide in primary nephrotic syndrome patients

Objective: To explore the mechanism of Buyang Huanwu Decoction (BYHWD) in treating primary nephrotic syndrome (PNS).Methods: Based on the treatment of prednisone acetate and cytoxan, two groups of PNS patients were treated with aspirin and persantin (western medicine group, WM, 35 patients) and BYHWD plus WM (TCM-WM group, 35 patients) respectively. The effect on anticoagulation was observed and compared. Plasma levels of thromboxane B₂ (TXB₂ ), 6-keto-prostaglandin F_1α (6-K-PGF_1α), endothelin (ET), calcitonin gene related peptide (CGRP) were determined before and after treatment, at the reducing dose and shifting to maintenance dose of prednisone. The therapeutic effect of the two groups were also observed. Another group of 30 healthy subjects was established for control.Results: The differences of TXB₂, 6-K-PGF_1α, ET and CGRP between patients and healthy subjects were very significant before treatment (P < 0. 001). The above-mentioned 4 parameters improved synchronously with the clinical improvement in the therapeutic course and they were better in the TCM-WM group than those in the WM group (P < 0.001 ), and the complete remission rate of the former group was also higher than that of the latter (62.9 % vs 37.1 %, χ² = 4.63, P < 0.05).Conclusion: BHD could improve the therapeutic effect in treating PNS through the mechanism of improving TXB₂, 6-K-PGF_1α, ET and CGRP levels.     

Clinial Implication of tThe Expression of Aurora B in Normal Endometrium and Endometrial Carcinoma

The expression of Aurora B in normal endometria and endometrial carcinomas and its relation with clinicopathologic parameters of endometrial carcinomas were investigated.Streptavidin-biotin peroxidase(SP) immunohistochemical technique was used to detect the expression of Aurora B in 10 cases of normal proliferative phase endometria,10 cases of normal secretory phase endometria and 72 cases of endometrial carcinomas respectively.According to the 1988 International Federation of Gynecology and Obstetrics(FIGO) grade,there were 37 patients in grade 1,23 in grade 2 and 12 in grade 3 respectively.According to the FIGO stage,there were 59 patients in stage Ⅰ-Ⅱ and 13 patients in stage Ⅲ-Ⅳ.Aurora B was expressed in both normal proliferative phase endometria,secretory phase endometria and endometrial carcinomas,but its positive labeling index(PLI) in proliferative phase endometria was significantly higher than that in secretory phase endo-metria(P<0.01) and endometrial carcinomas(P<0.01).The PLI of Aurora B was lower in tumors with well differentiation(G1),low surgical staging(Ⅰ-Ⅱ),and ≤1/2 myometrial invasion than that in tumors with moderate and low differentiation(G2-G3),higher surgical staging(Ⅲ-Ⅳ),and >1/2 myometrial invasion(all P<0.01).Aurora B exerts its functions in the replication of normal endometrial glandular cells;Expression of Aurora B is significantly correlated with biologic behavior of endo-metrial carcinoma,indicating that Aurora B may be a promising prognostic factor in endometrial carcinoma.     

Superparamagnetic Iron Oxide Labeling of Neural Stem Cells and 4.7T MRI Tracking in vivo and in vitro

Neural stem cells were labeled with superparamagnetic iron oxide (SPIO) and tracked by MRI in vitro and in vivo after implantation. Rat neural stem cells were labeled with SPIO combined with PLL by the means of receptor-mediated endocytosis. Prussian blue staining and electron mi-croscopy were conducted to identify the iron particles in these neural stem cells. SPIO-labeled cells were tracked by 4.7T MRI in vivo and in vitro after implantation. The subjects were divided into 5 groups, including 5×105 labeled cells cultured for one day after labeling, 5×105 same phase unla-beled cells, cell culture medium with 25 μg Fe/mL SPIO, cell culture medium without SPIO and dis-tilled water. MRI scanning sequences included T1WI, T2WI and T2WI. R2 and R2 of labeled cells were calculated. The results showed: (1) Neural stem cells could be labeled with SPIO and labeling efficiency was 100%. Prussian blue staining showed numerous blue-stained iron particles in the cyto-plasm; (2) The average percentage change of signal intensity of labeled cells on T1WI in 4.7T MRI was 24.06%, T2WI 50.66% and T2WI 53.70% respectively; (3) T2 of labeled cells and unlabeled cells in 4.7T MRI was 516 ms and 77 ms respectively, R2 was 1.94 s-1 and 12.98 s-1 respectively, and T2 was 109 ms and 22.9 ms, R2 was 9.17 s-1 and 43.67 s-1 respectively; (4) Remarkable low signal area on T2WI and T2WI could exist for nearly 7 weeks and then disappeared gradually in the left brain transplanted with labeled cells, however no signal change in the right brain implanted with unlabeled cells. It was concluded that neural stem cells could be labeled effectively with SPIO. R2 and R2 of labeled cells were increased obviously. MRI can be used to track labeled cells in vitro and in vivo.     

三种宫内节育器放置5年的临床效果比较

目的比较三种含铜宫内节育器放置的临床避孕效果。方法将自愿接受放环避孕的2593例妇女随机分为三组,分别放置元宫药铜(220)环809例,Tcu 220环880例,吉妮类环904例,随访5年,对比观察三组妇女的避孕效果、续用率以及副反应发生率。结果三组带器妊娠率、脱落率、因症取出率及续用率比较差异有统计学意义(P<0.05或0.01),其中吉妮类环的带器妊娠率、脱落率、因症取出率最低(P<0.01),续用率最高(P<0.01)。结论三种含铜宫内节育环的避孕效果均较良好,以吉妮类环最好,可推广应用。     

应用带铜节育器治疗继发性功能性闭经的35例临床观察

目的 探讨应用带铜节育器(IUD)治疗继发性功能性闭经的疗效.方法 2002年3月-2009年10月在本院门诊就诊的35例自愿参加治疗的继发性功能性闭经患者,闭经时间为4个月～3年,无过度肥胖和极度消瘦者,B超及盆腔检查子宫、卵巢无异常,血清促卵泡素(FSH)、黄体生成素(LH)、孕酮(P)、睾酮(T)、催乳素(PRL...     

A preliminary investigation on the possible association between diminished copper availability and non-alcoholic fatty liver disease in epileptic patients treated with valproic acid

Abstract

Background. Patients treated with valproic acid (VPA) present a high incidence of non-alcoholic fatty liver disease (NAFLD) (around 61%). Several recent studies suggest that low copper stores could be associated with NAFLD, and a significant decrease of copper availability in VPA-treated patients has been described.

Design and methods. In 101 adult epileptic patients treated with valproic acid in monotherapy (n = 75) and polytherapy (n = 26) the copper availability was evaluated using the specific oxidase activity of ceruloplasmin (activity per unit mass of enzyme protein) and the copper/ceruloplasmin ratio. Copper deficiency was supposed in the cases in which this biochemical variable was smaller than the lower reference limit (333 U/g).

Results. The differences between the groups of patients with ceruloplasmin oxidase activity smaller or greater than 333 U/g for the serum levels of aminotransferases, gamma-glutamyltransferase, butyrylcholinesterase, cholesterol, triglycerides, and C-reactive protein, and the APRI and FIB-4 liver fibrosis scores were not statistically significant. Most patients (93%) had low APRI and FIB-4 scores, suggesting absence of significant liver fibrosis.

Conclusions. The results obtained do not confirm the hypothesis of an association between diminished copper availability and NAFLD in patients treated with valproic acid.     

丹酚酸B对急性心肌缺血/再灌注损伤大鼠心肌保护作用的机制研究*

[目的]通过观察丹酚酸-B对心肌缺血再灌注损伤大鼠的实验观察探讨其作用机制.[方法]将实验动物分为5组,假手术组、模型组、卡托普利组、复方丹参片组、丹酚酸-B组分别给于不同处理因素.[结果]丹酚酸-B可降低RI大鼠血浆AT-1、ET、TXA2、TNF、Ca2+水平,提高PGI2、SOD水平,明显优于复方丹参片组及模型组(P＜0.05).[结论]丹酚酸-B具有较好的RI心肌细胞保护作用.     

放置不同类型宫内节育器的家兔子宫内膜组织化学观察

通过放置惰性宫内节育器(IUD)及3种不同类型活性IUD后,家兔子宫内膜酸性磷酸酶(ACP)及碱性磷酸酶(AKP)的组织化学观察,发现各置器组兔子宫内膜ACP活性均较自身对照子宫有所增高,以Cu-IUD组最为明显,且Cu-IUD置器组子宫ACP活性显著高于LNG-Cu-IUD置器组,提示LNG与Cu~(++)有一定的拮抗效应,为发展LNG-Cu-IUD提供了理论依据。在Cu-IUD及LNG-Cu-IUD置器组中,AKP活性虽有降低趋势,但无显著意义(P>0.05)。     

气候因素对青铜文物模拟材料大气腐蚀的影响

运用QCM反应性监测方法研究了暴露在博物馆模拟展柜中的青铜文物模拟材料的腐蚀行为及规律,并利用扫描电镜(SEM)、能谱仪(EDS)以及光电子能谱(XPS)分析手段,对青铜文物模拟材料在腐蚀60 d后的腐蚀形貌与产物进行了分析。结果表明:光照的存在以及温度、湿度的升高都会加速青铜文物模拟材料的腐蚀,其中ERCO LED灯在色温为4 000 K时对青铜文物模拟材料的腐蚀影响最严重,在腐蚀60 d后的主要腐蚀产物为Cu₂O、CuO、SnO、SnO₂,还有少量铜的碳酸盐、铜的硫酸盐与铜的硫化物、铜的硝酸盐与亚硝酸盐、铜的氯化物。     

瞬时受体电位阳离子通道亚家族C成员6对子宫内膜癌细胞周期的调控作用

目的 探讨子宫内膜癌组织中瞬时受体电位阳离子通道亚家族C成员6（TRPC6）的表达变化及其对子宫内膜癌细胞周期的调控作用。方法 应用qRT-PCR技术和蛋白质印迹法检测30例正常子宫内膜、30例不典型增生和32例子宫内膜癌组织中TRPC6的表达。分别用SKF59636（TRPC6阻断剂）和小干扰RNA（siRNA）干扰两种方法阻断TRPC6的作用,观察子宫内膜癌细胞HEC-1A细胞周期的变化。结果 子宫内膜癌组织中的TRPC6 mRNA和蛋白表达量均高于不典型增生组织和正常子宫内膜组织（P<0.01）。SKF96365呈剂量依赖性方式将细胞阻滞于G₂/M期,使G₀/G₁期细胞减少;转染siRNA可将子宫内膜癌细胞阻滞于G₂/M期,使G₀/G₁期细胞减少,同时上调磷酸化细胞分裂周期蛋白2（pCDC2）的表达。结论 子宫内膜癌组织中TRPC6表达增高,TRPC6可能通过影响pCDC2蛋白表达参与子宫内膜癌细胞周期调控。     

Predicting Endometrium Receptivity with Parameters of Spiral Artery Blood Flow

Endometrium receptivity refers to a statewhen endometrium allows a blastocyst to attach,penetrate and induce changes in the stroma whichresult in embryonici mplantation[1].It plays ani m-portant role in determining the outcomes of ARTtreat ment .In this study ,the difference of spiralartery bloodflowbetweeninfertile women who hadundergone ART treat ments with different out-comes (pregnancy and non-pregnancy) was retro-spectively analyzed to evaluate whether the param-eters of spiral artery b…     

一种新型锰金属有机框架纳米载体用于克服肿瘤缺氧增效光动力治疗

使用简单的溶剂法制备了一种新型的锰簇卟啉金属有机框架纳米载体(nMn-MOF),并对其体外增效光动力治疗的能力进行了考察。通过动态光散射仪、透射电镜、X射线光电子能谱对该纳米载体进行表征,采用氧探针、ICG荧光染料考察其催化过氧化氢产生氧气和光照下产生单线态氧(¹O₂)的能力。在细胞水平上,采用CCK-8法检测纳米载体的暗毒性和光毒性,通过荧光染色观察纳米载体改善肿瘤细胞乏氧和产生活性氧的能力。结果表明,制备的纳米级的锰簇卟啉金属有机框架纳米载体具有过氧化氢特异性产氧和产生高水平单线态氧的能力,改善肿瘤乏氧,从而增效光动力治疗。