髋关节置换术和骨折内固定术治疗老年股骨颈骨折疗效比较

目的比较人工髋关节置换术与骨折内固定术治疗老年股骨颈骨折疗效。方法将1996～2005年在我院治疗的32例57岁以上有移位的股骨颈骨折患者分为两组，A组为髋关节置换术治疗，B组为内固定术治疗，随访1—3年，平均24个月。结果两组在住院时间和伤后入院手术时间比较，差异无显著性（P〉0．05），而并发症的发生率（A组6％，B组31％），扶双拐下地活动时间（A组7—21天，B组60—90天）及功能评价（A组优良率94％，差6％，B组优良率69％，差31％），差异有显著性（P〈0．05）。结论髋关节置换术治疗老年股骨颈骨折的优点为：（1）避免了股骨颈骨折不愈合及股骨头坏死问题；（2）降低并发症的发生率；（3）治疗时间短；（4）提高患者的生活质量。     

动力髋部螺钉和股骨近端髓内钉与人工股骨头置换术治疗老年股骨转子间不稳定型骨折的疗效分析

目的探讨动力髋部螺钉（DHS）、股骨近端髓内钉（PFN）和人工股骨头置换术对老年股骨转子间不稳定型骨折的疗效。方法96例股骨转子间不稳定型骨折患者中,DHS组34例,PFN组36例,人工股骨头置换术组26例,对其术中、术后指标进行比较。结果96例患者随访10—38个月,平均18个月。3组术中出血量、手术时间、术后引流量、术后下床时间之间的差异有统计学意义。术后并发症以DHS组最多,PFN组次之,人工股骨头置换组最少。按Harris评分系统评估,各组优良率分别是79．4％（DHS组）、94．2％（PFN组）、92．3％（人工股骨头置换组）;后两组的优良率显著高于DHS组（P〈0．05）。结论对于老年股骨转子间不稳定型骨折的治疗,PFN和人工股骨头置换两种术式均行之有效,优于DHS,在严格掌握适应证的前提下,人工股骨头置换术后可早期下床行走,减少并发症,对高龄伴严重骨质疏松的患者尤为适宜。     

人工股骨头置换与DHS治疗高龄股骨粗隆间骨折

目的通过比较分析人工股骨头置换术与DHS内固定术治疗高龄股骨粗隆间粉碎骨折的疗效及预后，前瞻性评价加长柄骨水泥型人工股骨头置换术治疗高龄股骨粗隆唰粉碎骨折的效果。方法1999年6月至2005年6月间我科收治高龄股骨粗隆间骨折120例患者，男67例。女j3例。年龄75～94岁，平均81．4岁。120例分别用人工股骨头置换术与DHS内固定术治疗，其中加长柄骨水泥型人工股骨头置换术50例，DHS内固定术70例。骨折类型按Evans分型。Ⅲ型58例。Ⅳ型62例。结果110例患者得到近期随访。平均随访时间1．5年．10例DHS内固定患者失访。DHS内固定组有6例手术失败后，均重新用加长柄骨水泥人工股骨头置换术治疗。加长柄骨水泥人工股骨头置换术50例．术后随访期间患者仅有1例并发肺部感染。其余疗效满意。术后并发症两组患者比较差异有显著性意义（P〈0．05）。结论人工股骨头置换术将成为治疗高龄股骨粗隆间不稳定骨折的主要方法。     

单极与双极人工股骨头置换(用或不用骨水泥)治疗老年股骨颈骨折

目的：比较单极与双极人工股骨头置换术(用或不用含水泥)治疗老年股骨颈骨折的疗效。方法：自1990年1月至2001年12月住院治疗的股骨颈骨折病例中，选择年龄、性别、随访时间均相仿的病例分为四组。A组为骨水泥型双极人工股骨头置换术患者40例；B组为非骨水泥型双极人工股骨头置换术患者45例；C组为骨水泥型单极人工股骨头置换术患者42例，D组为非骨水泥型单极人工股骨头置换术患者45例，进行回顾性分析。结果：比较四组住院时间、扶双拐下地活动时间、出院时功能评估、远期并发症、髋臼磨损和再手术率，无显著性差异(P＞0．05)。A组和C组的手术时间长、失血量大，分别与B组和D组相比，有显著性差异(P＜0．05)。随访中发现双极假体置换A组和B组步速明显快于单极假体置换C组和D组，有显著性差异(P＜0．05)。未应用含水泥的B组和D组，患者镇痛率多于应用骨水泥的A组和C组，但并不影响治疗效果的评价。四组功能评价优良率为：A组90．0％，B组84．4％，C组88．1％，D组82．2％，无显著性差异(P＞0．05)。结论：人工股骨头置换术是治疗老年人股骨颈骨折的有效方法，可促使者年人早期活动，提高生活质量。本组资料表明四种置换方法无明显差异，对要求术后有较快步行能力的老年患者，应选择双极人工股骨头置换，如有明显骨质疏松和股骨上段髓腔骨皮质薄弱情况的老年患者，骨水泥的应用是必需的。     

切开复位内固定术治疗Pilon骨折的手术时机选择

目的探讨切开复位内固定术治疗Pilon骨折的最佳手术时机选择。方法自2008—05－2012—06收治的55例Pilon骨折按手术时机分为2组,A组27例于伤后3—7d行切开复位内固定术,B组28例延期于伤后7—21d待软组织肿胀消退后再进行切开复位内固定术。比较2组骨折愈合时间、术后并发症发生率和踝关节功能。结果A组骨折愈合时间平均（18．7±3．1）周,并发症发生率29．6％;B组骨折愈合时间平均（15．4±2．4）周,并发症发生率7．1％;B组骨折愈合时间少于A组,并发症发生率低于A组,差异有统计学意义（P〈0．05）。末次随访时,A组踝关节功能优良率74．1％,B组踝关节功能优良率89．3％,B组踝关节功能优良率高于A组,差异有统计学意义（P〈0．05）。结论Pilon骨折手术时机的选择对手术效果具有关键的影响,分步延期手术可以缩短骨折愈合时间,改善愈合效果,促进踝关节功能的进一步康复。     

人工股骨头置换与内固定治疗股骨转子间骨折疗效比较

目的探讨人工股骨头置换术与内固定术治疗股骨转子间骨折的疗效。方法74例股骨转子间骨折患者中25例行标准柄双极人工股骨头置换术，49例行Gamma钉或Richard钉内固定术，对其临床疗效进行比较。结果人工股骨头置换组：1例1个月后死亡，余随访7～34个月；除1例有轻度髋内翻外，余患者均疗效满意。患髋功能按Harris评分法：优18例，良5例，可1例，优良率92％（23／25）。内固定组：随访10～34个月，X线摄片提示全部骨性愈合，优40例，良6例，可3例，优良率93．9％（46／49）。Gamma钉组有1例术后第6个月时发生1枚螺钉穿破股骨头而引起行走痛，重新行人工全髋置换术，术后功能良好。结论股骨转子间骨折的手术治疗，两种治疗方式均行之有效，人工股骨头置换术患者术后可以早期下地行走，缩短卧床时间，对高龄伴严重骨质疏松的患者尤为适合，但需严格掌握适应证。     

高龄转子间骨折三种手术方式的选择

目的探讨用四部分骨折Singh指数分类法来指导高龄转子间骨折治疗方式的选择。方法回顾性分析我院1990-2004年有完整随访资料的412例患者，男性227例，女性185例；年龄71-92岁，平均年龄74.6岁。参照E-vans和肱骨近端四部分骨折的分类方法，将高龄转子间骨折分为四部分骨折（四型），术前用健侧髋关节X线片来测量Singh指数。骨水泥固定采用人工股骨头置换手术185例，动力髋螺钉（dynamic hip screw，DHS）内固定123例，Gamma钉内固定104例。结果经过12-24个月的随访，依据疗效评定标准判定术后下肢功能的优良率，人工股骨头置换组（A组）94.59％，DHS内固定组（B组）65.85％，Gamma钉内固定组（C组）75.00％。A组优良率明显高于B、C组（P〈0.01）。内固定或假体松动失效率，A组为0，B组Singh指数4-6级内固定的失败率24.52％，1-3级的失败率为61.49％，C组Singh指数4-6级内固定失败率为15.22％，1-3级内固定的失败率为27.95％。A组失败率明显低于B、C组（P〈0.01）；C组明显低于B组（P〈0.01）；B、C组内Singh指数4-6级组失败率明显低于Singh指数1-3级组（P〈0.05）。平均手术时间、失血量及术后下床活动时间A组明显少于B、C组（P〈0.01）。结论四部分骨折Singh指数分类法对高龄转子间骨折的治疗具有指导意义，三部分骨折（小转子明显移位）、四部分骨折Singh指数在4级以下的患者应首选骨水泥型人工股骨头置换手术。     

高龄不稳定股骨粗隆间骨折患者应用骨水泥型人工股骨头置换术治疗的临床效果分析

目的探讨骨水泥型人工股骨头置换术治疗高龄不稳定股骨粗隆间骨折的临床效果及安全性。方法选取2017-01—2019-04间杞县中医院收治的82例高龄不稳定股骨粗隆间骨折患者,随机分为2组,每组41例。分别予以股骨近端防旋髓内钉(PFNA)内固定术(对照组)和骨水泥型人工股骨头置换术(观察组)。结果观察组手术时间、术中出血量,以及术后并发症发生率、下床负重锻炼时间和髋关节功能均优于对照组,差异有统计学意义(P0.05)。结论骨水泥型人工股骨头置换术应用于高龄不稳定股骨粗隆间骨折患者,手术创伤少、术后并发症风险低,有利于促进患者早日下床负重锻炼和髋关节功能恢复。     

人工股骨头置换术与股骨近端防旋髓内钉内固定治疗老年骨质疏松患者股骨转子间骨折的疗效比较

【目的】比较分析人工股骨头置换(AFHR)与股骨近端防旋髓内钉（PFNA）内固定治疗老年骨质疏松患者股骨转子间骨折的术后疗效。【方法】前瞻性分析我院从2013年1月至2016年1月我院收治的108例股骨转子间骨折患者，根据入院顺序随机将患者分为A组（54例）和B组（54例），A组行人工股骨头置换术(AFHR)治疗，B组行股骨近端防旋髓内钉（PFNA）内固定治疗，并对患者随访1年。分别记录统计患者的住院时间、手术时间、术中出血量、术后引流量、术后下地时间、术后髋关节功能Harris评分及术后并发症发生情况。【结果】两组间平均手术时间无统计学差异（p>0.05），而术中出血量、术后引流量、及下地时间之间差异具有统计学意义；两组患者术后早期1、3月，Harris评分差异具有统计学意义（p<0.05），术后6、12个月时，两组间Harris评分差异无统计学意义（p>0.05）; 两组并发症发生率差异具有统计学意义。【结论】人工股骨头置换术(AFHR)具有术后卧床时间短，早期可下地活动开始功能锻炼，有效的防止了并发症的发生，并显著提高了老年患者的生活质量。因此，人工股骨头置换术(AFHR)是治疗老年骨质疏松患者股骨转子间骨折更为理想的治疗方法。     

人工股骨头置换术治疗高龄股骨转子间骨折

目的将高龄股骨转子间骨折的人工股骨头置换术与高龄股骨颈骨折行人工股骨头置换术的临床结果相比较,探讨人工股骨头置换术治疗高龄股骨转子间骨折的经验、适应证以及早中期随访结果。方法对1996年8月～2002年4月间行人工股骨头置换术治疗的80岁以上新鲜的40例股骨转子间骨折和45例股骨颈骨折高龄患者进行回顾性分析,将随访结果进行比较和评估,并进行统计学分析。结果两组随访时间均为3～7年,转子间骨折组平均4．6年,股骨颈骨折组平均5．5年。两组在住院时间、术中出血量、术后引流量、术后扶拐下地时间和术后早期并发症等方面比较,差异无显著性意义(P＞0．05)。转子间骨折组比股骨颈骨折组手术时间长,术后出现双下肢不等长的可能性高,差异有显著性意义(P＜0．05)。末次随访的Harris髋关节评分,两组比较差异无显著性意义(P＞0．05)。术后并发症情况：在深静脉血栓、髋关节间隙变化、疼痛、假体柄松动下沉等方面,两组比较差异无显著性意义(P＞0．05)。两组假体翻修率差异无显著性意义(P＞0．05),均未见因髋臼磨损而发生髋臼内陷甚至中心性脱位。结论使用标准骨水泥型股骨假体,股骨转子间骨折行人工股骨头置换术取得了与股骨颈骨折行人工股骨头置换术相同的临床效果。     

维生素D受体在糖尿病肾病足细胞损伤及蛋白尿缓解中的作用

目的探讨维生素D受体(VDR)在糖尿病肾病(DKD)足细胞中的表达水平及在足细胞损伤及蛋白尿缓解中的作用。方法(1)本研究纳入了65例诊断患有2型糖尿病(伴或不伴蛋白尿)的患者,并纳入了25例年龄和性别相匹配的健康体检者为对照组。根据白蛋白/肌酐(ACR)的尿排泄比例对2型糖尿病患者进行分组,分别为无蛋白尿(ACR<30 mg/g,n=24)、微量白蛋白尿(ACR 30~300 mg/g,n=18)和临床蛋白尿(ACR>300 mg/g,n=23)。另选择25例经肾活检确诊的DKD患者作为DKD组。正常肾脏组织标本均取自泌尿外科同一时期肾脏肿瘤切除患者10例。将各组检测指标进行对比,同时采用实时定量PCR、ELISA法和免疫组化法检测VDR在各组患者的血液、尿液样本和肾脏组织中的表达情况,以及使用Pearson相关分析分析VDR与尿蛋白的相关性。(2)在2型糖尿病肾病小鼠模型中对上述结果进行验证,将遗传背景均为C57BLKs/J的雄性db/db小鼠及同窝出生的db/m小鼠,随机分为正常对照组(A组)、DKD对照组(B组)、DKD二甲基亚砜处理组(C组)、DKD帕立骨化醇(VDR激动剂)处理组(D组),C、D组连续腹腔注射处理8周,对照组不做任何处理。小鼠10周龄时开始连续干预8周,在小鼠22周龄(开始干预后12周)检测各组小鼠体重、血、尿生化指标对比;Western印迹法检测β⁃catenin、VDR的变化;免疫荧光观察足细胞标志蛋白podocin及足细胞损伤蛋白α⁃SMA的表达变化。结果(1)与正常健康对照组相比,无蛋白尿组、微量白蛋白尿组和临床蛋白尿组的糖尿病患者血浆中VDR的mRNA和蛋白水平均较低(均P<0.05);与无蛋白尿组的糖尿病患者相比,微量白蛋白尿组和临床蛋白尿组的糖尿病患者血浆中VDR的mRNA和蛋白水平均较低(均P<0.05)。(2)与正常健康对照组相比,无蛋白尿糖尿病组和DKD组患者血浆中VDR的mRNA和蛋白水平均较低(均P<0.05);与无蛋白尿糖尿病组患者相比,DKD组患者血浆中VDR的mRNA和蛋白水平亦较低(均P<0.05)。(3)免疫组化结果显示,DKD组肾组织中VDR的表达明显少于正常对照组。(4)DKD患者血浆中VDR mRNA相对水平与ACR呈负相关(r=-0.342,P<0.05)。(5)各组尿液上清液中VDR的水平与血浆中的水平呈相反趋势。(6)Western印迹结果显示,B组、C组肾小球足细胞β⁃catenin蛋白表达高于D组(均P<0.05),VDR蛋白的表达低于D组(均P<0.05);免疫荧光结果显示,B组、C组肾小球足细胞podocin的表达低于D组(均P<0.05),α⁃SMA的表达高于D组(均P<0.05)。结论VDR高表达缓解DKD足细胞损伤及蛋白尿。     

Comparison of University of Wisconsin and University of Pittsburgh solutions for heart transplantation

The effectiveness of University of Wisconsin (UW) and University of Pittsburgh (UP) solutions for the preservation of rat hearts was compared. Lewis rat hearts were preserved with UW (group A, n=45) or UP (group B, n=45) solution for 0 or 24 h and then transplanted heterotopically into the recipients' abdomen. Ten recipients in each group were observed to obtain 1-week graft survival rates. Tissue water content and tissue content of adenine nucleotides were measured 2 h after transplantation in six grafts from each group. Six hearts preserved for 0 h and seven hearts preserved for 24 h were taken from each group 24 h after grafting for histopathology. The 1-week graft survival rates of groups A24 and B24 were 60% and 10%, respectively. In the 24-h preserved grafts, adenosine triphosphate (ATP) and energy charge [(ATP+adenosine diphosphate/2)/(ATP+adenosine diphosphate+adenosine monophosphate)] of groups A and B were 0.972±0.165 and 0.200±0.123 mg/g wet tissue (P<0.05) and 74.4% and 61.1% (P<0.05), respectively. The tissue water content of group A24 was 71.7%, whereas that of group B24 was 74.1% (P<0.05). Histopathology revealed more severe muscle edema and necrosis and infiltration of polymorphonuclear cells in group B24 than in group A24. We conclude that UW solution is more appropriate for rat heart preservation than UP solution.     

Renal allograft rejection: the temporal relationship and predictive value of plasma TNF (alpha and beta), IFN-gamma and soluble ICAM-1

Recently, close interactions have been described between the tumour necrosis factors alpha and beta (TNF-alpha and beta), interferon-gamma (INF-gamma) and intercellular adhesion molecule-1 (ICAM-1) in T-cell mediated immune activation. During the process of renal graft rejection, the properties of these cytokines to act as powerful stimulators of macrophages, to upregulate class II MHC expression and to stabilise cell-to-cell binding make them of great potential interest. The aim of the present study was to determine the plasma levels of each cytokine and soluble ICAM-1 in 16 renal allograft recipients. We examined plasmas of patients for the first 2 weeks after transplantation and correlated results with the clinical pattern of rejection. Our data suggest an immunopathologic involvement of TNF-alpha, TNF-beta and slCAM-1 in renal allograft rejection and showed that there was a significant elevation in plasma concentrations of these parameters 2 or 3 days prior to the diagnosis of clinical rejection. Rises in INF-gamma did not appear to be significant with regard to rejection as very high levels were found in patients showing no evidence of clinical rejection.     

移植肾IgA肾病临床病理及预后分析

目的探讨移植肾IgA肾病(IgAN)复发或新发的诱因及移植肾生存的危险因素。方法选取2012年11月至2018年12月浙江大学医学院附属第一医院经肾活检确诊为移植肾IgAN的患者,按照血肌酐(Scr)增高水平、估算肾小球滤过率(eGFR)下降率分为稳定组(Scr升高值<20μmol/L,eGFR下降率<10%)和进展组(Scr增高但未达翻倍值,30%相似文献   

Osteoporosis and obesity: Role of Wnt pathway in human and murine models

Studies concerning the pathophysiological connection between obesity and osteoporosis are currently an intriguing area of research.Although the onset of these two diseases can occur in a different way,recent studies have shown that obesity and osteoporosis share common genetic and environmental factors.Despite being a risk factor for health,obesity has traditionally been considered positive to bone because of beneficial effect of mechanical loading,exerted by high body mass,on bone formation.However,contrasting studies have not achieved a clear consensus,suggesting instead that excessive fat mass derived from obesity condition may not protect against osteoporosis or,even worse,could be rather detrimental to bone.On the other hand,it is hitherto better established that,since adipocytes and osteoblasts are derived from a common mesenchymal stem cell precursor,molecules that lead to osteoblastogenesis inhibit adipogenesis and vice versa.Here we will discuss the role of the key molecules regulating adipocytes and osteoblasts differentiation,which are peroxisome proliferators activated receptor-γand Wnts,respectively.In particular,wewill focus on the role of both canonical and non-canonical Wnt signalling,involved in mesenchymal cell fate regulation.Moreover,at present there are no experimental data that relate any influence of the Wnt inhibitor Sclerostin to adipogenesis,although it is well known its role on bone metabolism.In addition,the most common pathological condition in which there is a simultaneous increase of adiposity and decrease of bone mass is menopause.Given that postmenopausal women have high Sclerostin level inversely associated with circulating estradiol level and since the sex hormone replacement therapy has proved to be effective in attenuating bone loss and reversing menopause-related obesity,we hypothesize that Sclerostin contribution in adipogenesis could be an active focus of research in the coming years.     

Evaluation and comparison of the pharmacokinetic and pharmacodynamic properties of allopregnanolone and pregnanolone at induction of anaesthesia in the male rat

We have evaluated and compared the pharmacokinetic and pharmacodynamicproperties of allopregnanolone and pregnanolone at inductionof anaesthesia in male rats. A threshold method was used, andthe first burst suppression period of 1 s or more in theEEG was selected as the end-point after fairly slow infusions.An optimal dose of 4.0 mg kg^–1 min^–1was noted for both steroids. Brain concentrations were low atlow infusion rates, indicating that acute tolerance was notoccurring. Significant positive correlations were noted betweendose rate and serum concentrations of allopregnanolone (r =0.94, P<0.001) and pregnanolone (r = 0.88, P<0.001). Suchcorrelations were also seen in striatum, cerebellum, cortexand muscle for both steroids (P<0.01). Despite changing infusionrates, the concentrations of both steroids in brainstem, hippocampusand fat remained stable. Because no correlation between infusionrate and steroid concentration was noted in the brainstem andhippocampus, these two brain areas may be regarded as primarysites of action for allopregnanolone and pregnanolone. Pregnanoloneconcentrations in the brainstem and hippocampus were significantlyhigher than those of allopregnanolone, suggesting that allopregnanolonewas more potent than pregnanolone in inducing anaesthesia. Br J Anaesth 2001; 86: 403–12     

Role of Conventional and Diffusion-Weighted Magnetic Resonance Imaging of Spinal Treatment Protocol for Hydatid Disease

Objective:

To demonstrate the role of magnetic resonance imaging (MRI) in determining the treatment protocol for hydatid disease of the spine.

Design:

Case report; literature review.

Findings:

Diffusion-weighted MRI can help differentiate complicated infected hydatidosis from abscesses, epidermoid cysts from arachnoid cysts, and benign from malignant vertebral compression fractures. It is also helpful in differentiating between abscesses and necrotic tumors.

Conclusion:

Diffusion-weighted MRI can help differentiate between infections requiring immediate surgery and those that can be treated medically with antihelmintic treatment.     

末端可弯输尿管肾镜与电子输尿管软镜治疗肾结石的比较

目的比较末端可弯曲输尿管肾镜（孙氏镜）与电子输尿管软镜联合钬激光治疗肾结石的疗效、安全性及优缺点。 方法回顾性分析我院2015年9月至2017年3月应用孙氏镜与电子输尿管软镜联合钬激光治疗120例肾结石患者临床资料,其中65例采用电子输尿管软镜（电子镜组）,55例应用孙氏镜（孙氏镜组）,比较两组的临床资料及碎石效果。 结果两组手术时间、并发症、术后平均住院时间以及清石率等比较差异均无统计学意义（P>0.05）,总住院时间、治疗费用比较差异有统计学意义（P<0.05）。 结论孙氏镜联合钬激光治疗肾结石,视野清晰度较电子输尿管软镜略有不足,但操作更简便,微创、安全、有效,总住院时间短,费用低,值得应用和推广。     

Prevention of postoperative nausea and vomiting after spinal morphine for Caesarean section: comparison of cyclizine,dexamethasone and placebo

Background. Low-dose intrathecal (spinal) morphine (0.1–0.2mg) for Caesarean section delivers excellent postoperative analgesiabut is associated with significant nausea and vomiting. We comparedthe antiemetic efficacy of cyclizine, dexamethasone, and placeboin this clinical setting. Methods. Ninety-nine women undergoing elective Caesarean sectionunder spinal anaesthesia were allocated randomly, in a double-blindstudy design, to receive either cyclizine 50 mg, dexamethasone8 mg, or placebo as a single-dose infusion in saline 0.9%, 100ml on completion of surgery. Spinal anaesthesia consisted of:hyperbaric bupivacaine 0.5%, 2.0 ml; fentanyl 10 µg; andspinal morphine 0.2 mg. The primary outcome measure was theincidence of nausea. Results. The incidence of nausea was significantly less in patientsreceiving cyclizine compared with dexamethasone and placebo(33 vs 60 and 67%, respectively, P<0.05). Severity of nauseaand number of vomiting episodes were also less at 3–6h in cyclizine patients. Overall satisfaction with postoperativecare at 24 h, expressed on a 100 mm visual analogue scale, wasgreater in cyclizine [78 (28)] than either dexamethasone [58(31), P=0.03] or placebo [51 (28), P=0.008]. Conclusion. We conclude that following spinal morphine 0.2 mgand fentanyl 10 µg analgesia for Caesarean section, cyclizine50 mg i.v. reduces the incidence of nausea compared with dexamethasone8 mg i.v. or placebo. It also lessens the severity of nauseaand vomiting, and increases maternal satisfaction in the earlypostoperative period. Br J Anaesth 2003; 90: 665–70     

Ultrastructural Characteristics of Dog and Human Platelets Adherent to Native and Collagen-Coupled Cuprophan

Adhesion of human and dog platelets to native and collagen-coupled Cuprophan under defined flow conditions was studied by scanning and transmission electron microscopy. Dog platelets, singly adherent to and uniformly distributed on both native and collagen-coupled Cuprophan, extend slender pseudopods across the surface without evidence of degranulation. Human platelets, while not adhering to native Cuprophan, formed irregularly shaped, semi-confluent cytoplasmic sheets on the collagen-coupled surface. Extensive cytoplasmic reorganization and degranulation suggests a post-release state of the human platelets. Aspirin had no apparent effect on either human or dog platelet adhesion or upon the apparent release state of the human platelets.