瑞芬太尼诱发切口痛大鼠痛觉过敏时脊髓iNOS mRNA和FOS蛋白表达的变化及曲马多对其的影响

目的 通过评价瑞芬太尼诱发切口痛大鼠痛觉过敏时脊髓诱导型一氧化氮合酶(iNOS)mRNA及FOS蛋白表达的变化,探讨瑞芬太尼诱发痛觉过敏的机制及曲马多对其的影响.方法 健康雄性SD大鼠48只,体重200～250g,随机分为4组(n=12),对照组(C组)不行切口操作,腹部皮下输注生理盐水0.4 ml,同时单次皮下注射生理盐水0.1 ml;切口痛组(I组)切皮后腹部皮下输注生理盐水0.4 ml,同时单次皮下注射生理盐水0.1 ml;瑞芬太尼组(R组)切皮后腹部皮下输注瑞芬太尼40 μg/kg(0.4 ml),同时单次皮下注射生理盐水0.1 ml,瑞芬太尼+曲马多组(R+T组)切皮后腹部皮下输注瑞芬太尼40 μg/kg(0.4 ml),同时单次皮下注射曲马多30 mg/kg(0.1 ml).各组腹部皮下输注时间为30min(0.8ml/h).分别于术前24 h、术后24、48 h测定机械痛阈,随后测定脊髓iNOS mRNA和FOS蛋白的表达水平.结果 与术前24 h比较,术后I组、R组和R+T组机械痛阈降低(P＜0.05);与C组比较,I组、R组和R+T组机械痛阈降低(P＜0.05);与I组比较,R组机械痛阈降低;与R组比较,R+T组机械痛阈升高(P＜0.05).与C组和I组比较,R组和R+T组脊髓iNOS mRNA和FOS蛋白表达上调(P＜0.05);与R组比较,R+T组脊髓iNOS mRNA和FOS蛋白表达下调(P＜0.05).结论 瑞芬太尼诱发切口痛大鼠痛觉过敏的机制可能与上调脊髓iNOS mRNA和FOS蛋白表达有关,曲马多可通过下调脊髓iNOS mRNA和FOS蛋白表达预防瑞芬太尼诱发的痛觉过敏.     

脊髓背角SPARCL1在瑞芬太尼诱发切口痛小鼠痛觉过敏形成中的作用

目的评价脊髓背角富含半胱氨酸的酸性分泌蛋白类似蛋白1(SPARCL1)在瑞芬太尼诱发切口痛小鼠痛觉过敏形成中的作用。方法健康雄性C57BL/6J小鼠48只,8～10周龄,体重18～22 g,采用随机数字表法分为6组(n=8):对照组(C组)、切口痛组(I组)、瑞芬太尼组(R组)、切口痛+瑞芬太尼组(I+R组) 、切口痛+瑞芬太尼组+阴性对照siRNA组(I+R+N组)和切口痛+瑞芬太尼+ SPARCL1-siRNA组(I+R+S组)。I+R+N组和I+R+S组分别鞘内注射1×108 IFU/ml阴性对照siRNA和SPARCL1-siRNA 10 μl,C组、I组、R组、I+R组鞘内注射生理盐水10 μl,6组均注射1次/d,连续3 d。待稳定转染后,C组和I组尾静脉注射生理盐水0.1 ml,R组、I+R组、I+R+N组和I+R+S组尾静脉注射瑞芬太尼10 μg/kg(用生理盐水稀释至0.1 ml),6组均连续注射4次,间隔15 min。I组、I+R组、I+R+N组和I+R+S组于第1次尾静脉给药后制备切口痛模型。分别于输注生理盐水或瑞芬太尼前24 h(T0)、输注停止后3、6、24和...     

脊髓P2Y1R在瑞芬太尼诱发切口痛大鼠痛觉过敏形成中的作用：与脊髓NR1和NR2B功能的关系

目的评价脊髓P2Y1嘌呤受体(P2Y1R)在瑞芬太尼诱发切口痛大鼠痛觉过敏形成中的作用及其与脊髓NMDA受体(NR)1和NR2B功能的关系。方法鞘内置管成功的健康成年雄性SD大鼠48只, 10～12周龄, 体重250～280 g, 采用随机数字表法分为6组(n=8):对照组(C组)、P2Y1R拮抗剂MRS2179组(M组)、瑞芬太尼组(R组)、瑞芬太尼+MRS2179组(R+M组)、切口痛+瑞芬太尼组(I+R组)和切口痛+瑞芬太尼+MRS2179组(I+R+M组)。C组鞘内注射生理盐水10 μl, 10 min后经尾静脉输注生理盐水0.1 ml·kg-1·min-1 60 min;M组鞘内注射MRS2179 0.6 nmol/kg, 10 min后经尾静脉输注生理盐水0.1 ml·kg-1·min-1 60 min;R组鞘内注射生理盐水10 μl, 10 min后经尾静脉输注瑞芬太尼1 μg·kg-1·min-1 60 min;R+M组鞘内注射MRS2179 0.6 nmol/kg, 10 min后经尾静脉输注瑞芬太尼1 μg·kg-1·min-1 60 min;I+R组鞘内注射生理盐...     

背根神经节S1PR1在瑞芬太尼诱发切口痛大鼠痛觉过敏中的作用

目的评价背根神经节鞘氨醇-1-磷酸-1受体(S1PR1)在瑞芬太尼诱发切口痛大鼠痛觉过敏中的作用。方法取鞘内置管和尾静脉置管成功的雄性SD大鼠48只, 体质量260～280 g, 2～3月龄, 采用随机数字表法分为6组(n=8):对照组(C组)、S1PR1拮抗剂组(F组)、瑞芬太尼组(R组)、瑞芬太尼+ S1PR1拮抗剂组组(R+F组)、瑞芬太尼+切口痛组(R+I组)和瑞芬太尼+切口痛+ S1PR1拮抗剂组(R+I+F组)。C组尾静脉输注生理盐水0.1 ml·kg-1·min-1 60 min;R组尾静脉输注瑞芬太尼1.0 μg·kg-1·min-1 60 min;F组鞘内注射FTY720 3 noml, 10 min后尾静脉输注生理盐水1.0 μg·kg-1·min-1 60 min;R+F组鞘内注射FTY720 3 nmol, 10 min后尾静脉输注瑞芬太尼1.0 μg·kg-1·min-1 60 min;R+I组建立切口痛模型的同时尾静脉输注瑞芬太尼1.0 μg·kg-1·min-1 60 min;R+I+F组鞘内注射FTY720 3 nmol, 10 min后建立切口痛模型,...     

氯胺酮对瑞芬太尼诱发切口痛大鼠痛觉敏化的干预效应

目的 探讨瑞芬太尼对大鼠切口痛的痛觉敏化作用及氯胺酮的干预效应.方法 选用健康雄性SD大鼠32只,随机分为四组:对照组(C组)静脉注射生理盐水10 ml,90 min后做切口痛模型;瑞芬太尼组(R组)持续泵注瑞芬太尼0.8 μg·kg-1 ·min-1,90 min后做切口痛模型;氯胺酮预处理组(K组)静脉推注氯胺酮8 mg/kg,90 min后做切口痛模型;联合处理组(KR组)静脉推注氯胺酮8mg/kg,30 min后泵注瑞芬太尼0.8μg·kg-1·min-1,60 min后做切口痛模型.采用热痛仪和von Frey纤毛测定各组大鼠的热痛阈和机械痛阈值.结果 术后2h各组热痛阈值和机械痛阈值显著降低(P＜0.05),R组明显低于其他组(P＜0.05);术后120 h,各组热痛阈值较术前降低(P＜0.05),C组与K组机械痛阈明显下降(P＜0.05).结论 瑞芬太尼静脉输注可引起大鼠切口痛痛觉敏化.小剂量氯胺酮预处理无术后镇痛作用,但氯胺酮预处理可拮抗瑞芬太尼诱发的大鼠切口痛痛觉敏化作用.     

甘氨酸转运体-1在大鼠切口痛模型瑞芬太尼痛觉敏化中的作用

目的 探讨甘氨酸转运体-1 (glycine-transporter 1,Gly-T1)抑制剂肌氨酸(sarcosine,Sar)在大鼠切口痛模型中对瑞芬太尼痛觉敏化效应的影响. 方法 雄性Sprague-Dawley(SD)大鼠36只,采用随机数字表法将其随机分为:瑞芬太尼+鞘内生理盐水组(RN组)、瑞芬太尼+鞘内肌氨酸组(RS组)、生理盐水+鞘内生理盐水组(NN组)、生理盐水+鞘内肌氨酸组(NS组),每组9例,按分组分别在切口痛术前给予瑞芬太尼或生理盐水尾静脉泵注,术后第1天给予肌氨酸或生理盐水鞘内注射.实验中采用VonFrey法分别测定各组大鼠术前机械缩足反射阈值(paw withdrawal mechanical threshold,PWMT)及术后2、4、12h,第1、3、5、7天的PWMT. 结果 与NN组比较,RN组的PWMT在术后2、4、12h及术后第1天分别为(0.21±0.04)、(0.63±0.21)、(1.33±0.50)、(3.31±1.03)g,显著降低(P＜0.01).而RS组在上述时间点与RN组比较,PWMT分别为(2.11±1.17)、(2.89±1.05)、(4.31±1.57)、(7.11±1.76)g,明显增高(P＜0.01),且与NN组比较差异无统计学意义(P＞0.05). 结论 瑞芬太尼具有致切口痛大鼠的痛觉敏化作用,该作用可被Gly-T1抑制剂——Sar所减轻,故Gly-T1可能参与了切口痛模型大鼠瑞芬太尼痛觉敏化的效应机制.     

脊髓谷氨酸转运体、孤啡肽和脑源性神经营养因子在炎性痛大鼠吗啡耐受形成中的作用

目的 探讨脊髓谷氨酸转运体(GT)、孤啡肽(OFQ)和脑源性神经营养因子(BDNF)在炎性痛大鼠吗啡耐受形成中的作用.方法 健康雄性SD大鼠,8～ 10周龄,体重300 ～ 350 g,取鞘内置管成功的雄性SD大鼠20只,采用随机数字表法,将其随机分为4组(n=5):生理盐水组(NS组)、炎性痛组(IP组)、吗啡组(M组)和GT激动剂riluzole组(R组).NS组于左后足踝关节腔注射生理盐水50μl,其余3组注射完全弗氏佐剂50 μl.3d后,NS组和IP组鞘内注射生理盐水10 μl;M组鞘内注射吗啡10 μg;R组鞘内注射riluzole 10 μg,30 min后注射吗啡10 μg.注射药物容量均为10μl,2次/d,连续7d.于鞘内给药前、鞘内给药2、4、6d和鞘内给药结束后1 d(T0-4)时测定机械痛阈.于T4时痛阈测定后取左侧脊髓背角,采用RT-PCR法测定OFQ mRNA和BDNF mRNA的表达.结果 与NS组比较,IP组机械痛阈降低,脊髓背角OFQ mRNA及BDNF mRNA表达上调(P＜0.05或0.01);与IP组比较,M组T1,2时机械痛阈升高,脊髓背角OFQ mRNA及BDNF mRNA表达上调(P＜0.05或0.01),T3,4时机械痛阈差异无统计学意义(P＞0.05);与M组比较,R组机械痛阈升高,脊髓背角OFQ mRNA及BDNF mRNA表达下调(P＜0.05或0.01).结论 炎性痛大鼠长期注射吗啡时脊髓GT功能降低,导致谷氨酸水平升高和OFQ、BDNF表达上调之间的失衡,可能在吗啡耐受形成中起到一定作用.     

切口痛-瑞芬太尼痛觉过敏大鼠脊髓总蛋白及膜蛋白NMDA受体NRl 、NR2A及NR2B亚基表达的变化

目的 评价切口痛-瑞芬太尼痛觉过敏大鼠脊髓总蛋白及膜蛋白NMDA受体NR1、NR2A及NR2B亚基表达的变化.方法 尾静脉置管成功的雄性SD大鼠32只,体重240～260 g,月龄2～3月,采用随机数字表法,将大鼠随机分为4组(n=8):对照组(C组)静脉输注等容量生理盐水60 min,瑞芬太尼组(R组)静脉输注瑞芬太尼1.2μg·kg-1·min-1 60 min;切口痛组(I组)建立切口痛模型,同时静脉输注等容量生理盐水60 min;瑞芬太尼+切口痛组(R+I组)建立切口痛模型,同时静脉输注瑞芬太尼1.2 μg· kg-1 ·min-1 60 min.于生理盐水或瑞芬太尼给药前24h、给药后2、6、24和48 h时测定机械刺激缩足阈值(PWT)和热刺激缩足潜伏期(PWL),最后一次测定痛阈后处死取脊髓L4～6节段,采用Western blot法测定脊髓总蛋白及膜蛋白NMDA受体NR1、NR2A及NR2B亚基的表达,并计算膜蛋白中NR2B/NR2A比值.结果 与C组比较,I组、R组和R+I组PWT降低,PWL缩短,总蛋白及膜蛋白NMDA受体NR1和NR2B亚基表达上调,膜蛋白中NR2B/NR2A比值增加(P＜0.05).与R组和I组比较,R+I组PWT降低,PWL缩短,总蛋白及膜蛋白NMDA受体NR1和NR2B亚基表达上调,膜蛋白中NR2B/NR2A比值增加(P＜0.05).各组总蛋白及膜蛋白NMDA受体NR2A亚基表达差异无统计学意义(P＞0.05).结论 大鼠切口痛-瑞芬太尼痛觉过敏的形成可能与脊髓总蛋白及膜蛋白NMDA受体NR1和NR2B亚基表达上调和膜蛋白中NMDA受体NR2B亚基组成比例的增加有关.     

大鼠切口痛模型中鞘内注射不同剂量KN93对瑞芬太尼诱发的痛觉过敏的影响

目的 观察不同剂量KN93对瑞芬太尼痛觉过敏大鼠痛行为的影响. 方法 采用完全随机分组方法,将36只雄性SD大鼠分为6组(每组6只):①空白对照组;②切口组;③瑞芬太尼组;④KN93 25 μg组;⑤KN93 50 μg组;⑥KN93 100 μg组.除空白对照组外,其余各组均制作切口痛模型、瑞芬太尼组及KN93 3个剂量组,手术过程中皮下输注瑞芬太尼(40 μg/kg,1 mg溶于40 ml生理盐水中)30 min.各组大鼠分别于术前24h、术后2、6、24、48 h测定术侧机械缩足阈值(paw withdrawal mechanical threshold,PWMT)和热缩足潜伏期(paw withdrawal thermal latency,PWTL). 结果 与切口组比较,瑞芬太尼组术后出现痛觉过敏表现PWMT (20.9±2.6,19.6±1.8,21.9±2.0,22.3±1.9)及PWTL[(8.5±1.5)s,(8.6±1.5)s,(8.5±2.0)s,(9.1±1.5)s],P＜0.05.鞘内注射KN93 25 μg对痛觉过敏大鼠痛行为无改善作用,鞘内注射KN93 50、100 μg能剂量依赖性改善瑞芬太尼所致痛觉过敏大鼠痛行为,P＜0.05. 结论 瑞芬太尼诱发切口痛大鼠痛觉过敏;KN93剂量依赖性的改善瑞芬太尼诱发的痛觉过敏.     

大鼠鞘内注射JWH015对瑞芬太尼诱发痛觉过敏的影响

目的 在大鼠切口痛模型基础上探讨大麻素受体2(cannabinoid receptor 2,CB2R)激动剂JWH015对瑞芬太尼诱发的痛觉过敏的影响.方法 30只雄性Sprague-Dawley(SD)大鼠采用随机数字表法分成5组(每组6只):对照组(C组)、切口痛组(I组)、瑞芬太尼组(R组)、切口痛+JWH015组(JI组)、切口痛+瑞芬太尼+JWH015组(JR组).JI组与JR组在造模前30 min鞘内注射10 μg JWH015,而C组、Ⅰ组、R组均给予20％的二甲基亚砜(dimethyl sulfoxide,DMSO)溶液,容积均为10μl.除C组外,其余各组均制作切口痛模型,R组和JR组在造模的同时皮下泵注瑞芬太尼0.04 mg/kg,其余组皮下泵注生理盐水,容积均为0.4 ml,30 min泵完.测定术前24h及术后2、6、24和48 h大鼠切口手术同侧后爪的机械缩足反射阈值(paw withdrawal mechanical threshold,PWMT)和热缩足反射潜伏期(paw withdrawal thermal latency,PWTL). 结果 与C组和基础值比较,Ⅰ 组术后各时间点PWMT和PWTL均降低(P＜0.05);与Ⅰ组比较,R组术后各时间点PWMT(6.3±0.8)、(6.3±0.8)、(6.3±1.0)、(6.8±0.9)g和PWTL (12.8±1.2)、(12.2±0.9)、(13.4±1.1)、(13.5±1.3)s均明显降低(P＜0.05);与R组相比,JR组术后6、24和48 h的PWMT(7.9±1.0)、(9.9±1.1)、(8.4±1.1)g和PWTL(17.3±1.9)、(19.9±1.3)、(17.7±1.2)s明显升高(P＜0.05).结论 鞘内注射JWH-015可以有效缓解由瑞芬太尼诱发的切口周围组织痛觉过敏.     

糖尿病性勃起功能障碍的血管因素

糖尿病性勃起功能障碍(DED)是一种由多因素引起的综合病症,其中血管内皮系统的损害起着重要作用。在糖尿病发展过程中,一些血管舒缩因子和生长因子有着显著的改变。这些改变与DED的发生存在明显的相关性。     

Changes in gene expression of growth factors and their receptors during castration-induced involution and androgen-induced regrowth of rat prostates

To find candidates for the mediator of the growth-promoting action of androgen in rat prostates, the changes in the steady-state levels of mRNAs coding for several growth factors and their receptors were examined by Northern blot analysis during castration-induced involution, and subsequent regrowth induced by androgen in the ventral and dorsolateral lobes. The changes in the growth factor systems and a typical secretory protein in the ventral lobe were similar to, but more prominent than, those in the dorsolateral lobe, showing the higher androgen dependency of the ventral lobe. Among the growth factors and their receptors investigated, only epidermal growth factor (EGF) showed apparent positive androgen dependency: EGF mRNA content in the ventral lobe decreased to about 30% of the normal level within 24 hr after castration, and increased, attaining about 200–300% of the normal level 3–5 days after androgen administration to castrated rats. mRNAs coding for all other factors examined, i.e., transforming growth factor-α (TGF-α), EGF receptor, basic fibroblast growth factor (bFGF), keratinocyte growth factor (KGF), FGF receptor 1, TGF-β1, TGF-β type II receptor, hepatocyte growth factor (HGF), and c-MET/HGF receptor, increased after castration in greater or lesser degree, and after a brief pause or a decrease some of them increased again attaining a second peak 3–5 days after androgen replacement. The second increase was evident in TGF-α, EGF receptor, KGF, and c-MET mRNAs. These results indicate the possibility that multiple growth factor-receptor systems participate in the androgen-dependent regrowth of castrated rat prostates. © 1996 Wiley-Liss, Inc.     

Influence of Patient-, Design-, and Surgery-Related Factors on Rate of Dislocation After Primary Cementless Total Hip Arthroplasty

We performed clinical, radiographic, and computed tomography examinations on a consecutive series of 1268 patients (1648 hips) to determine the prevalence of and factors contributing to dislocation after using a primary cementless total hip system. The prevalence of posterior dislocation was 3.6% (60 hips). Significant risk factors (Fisher exact test or χ² test, P < .05) were female sex, advanced age, high American Society of Anesthesiologists score (3 or 4), fracture of the femoral neck, nonrepair of the posterior soft-tissue sleeve, low or high cup anteversion, low or high stem anteversion, and low height of hip rotation center.     

Polypeptide growth factors and the kidney: a developmental perspective

A variety of polypeptides with stimulatory or inhibitory effects on cell proliferation have been identified. In addition to stimulating or inhibiting the proliferation of cells and maintaining their viability, polypeptide growth factors play significant roles in embryogenesis and differentiation. The current review focuses on five specific polypeptide growth factor families (epidermal growth factor, insulin-like growth factors, transforming growth factors, platelet-derived growth factor, and fibroblast growth factors) and discusses their possible relationship to normal renal physiology, abnormal renal pathophysiology, and renal organogenesis. On the basis of current data, it is clear that polypeptide growth factors are multifunctional agents with important effects on renal function and renal organogenesis.     

Activity of Keratinocyte Growth Factor-Like Substance Extracted from Rabbit Liver on Renal Tubular Cell Growth during Compensatory Renal Hyperplasia

Background : In response to unilateral nephrectomy, the rabbit liver transiently produces 2 growth regulators for cultured renal cortical tubular cells: a tubular cell growth factor and a growth inhibitor. We report on the effects of the tubular cell growth factor on a variety of cell lines.
Methods : The tubular cell growth factor activity was partially purified from the rabbit liver by using gel filtration and ion-exchange chromatography. The activity was monitored by the incorporation of iododeoxyuridine into DNA of cultured cells. Expression of the fibroblast growth factor receptor-2 in the rabbit kidney was determined by the immunoblot analysis.
Results : This growth factor stimulated the DNA synthesis in LLC-PK, cells, LLC-RK, cells, and human keratinocytes. It did not affect the growth of BS-C-1 cells, MDCK cells, BALB/c 3T3 fibroblasts, or rat parenchymal hepatocytes. The additive effect of this factor on the DNA synthesis of cultured tubular cells maximally was stimulated by insulin-like growth factor-l, basic fibroblast growth factor, and epidermal growth factor, but was not stimulated by keratinocyte growth factor. The amount of this activity also increased in the liver after sham operation. In the days after surgery, expression of fibroblast growth factor receptor-2, which includes the keratinocyte growth factor receptor, was down-regulated in the kidneys of both uninephrectomized and sham-operated rabbits.
Conclusion : These findings indicate that tubular cell growth factor in the liver seems to be a keratinocyte growth factor, and acts in an endocrine manner in renal tubular hyperplasia.     

Changes of clotting factors (7, 9 and 10) and hepatocyte growth factor in patients with thrombotic microangiopathy after bone marrow transplantation

To investigate risk factors for thrombotic microangiopathy (TMA) after bone marrow transplantation (BMT), the levels of three clotting factors (7, 9 and 10) and hepatocyte growth factor (HGF) were measured. Among 46 consecutive patients who underwent BMT, six developed TMA and 40 did not. The levels of the clotting factors and HGF did not differ significantly between the six patients with TMA and the 40 patients without it. In two patients who developed TMA during the earlyperiod after BMT, however, the levels of the three clotting factors were significantly decreased even before BMT, along with a significant increase of HGF. These findings suggest that patients with severe hepatic dysfunction before BMT, especially those with impaired protein synthesis, had an increased risk of developing TMA soon after BMT. It was also suggested that measurement of clotting factors (7, 9 and 10) and HGF may be useful to predict the occurrence of TMA in the early period after BMT.     

CRF及其受体在胃肠疾病中的研究进展

促肾上腺皮质激素释放因子(crticotropin releasing factor,CRF)是一种重要的神经内分泌肽.CRF在体内分布广泛,主要作用为促进腺垂体合成与释放促肾上腺皮质激素,与内分泌、神经化学、行为等多种生理反应有关,广泛参与消化管生理和病理活动的调控.CRF引起胃肠动力功能改变最常见的类型是胃排空延迟、延缓小肠蠕动及结肠运转加快.CRF通过CRFR2作用抑制胃排空,而刺激结肠动力是通过CRFRI调节的.CRF受体拮抗剂的研发为治疗应激相关胃肠疾病可能开辟新方向.     

全麻下血管化游离皮瓣修复术后患者谵妄的危险因素

Objective To investigate the risk factors for postoperative delirium in patients after vascular free flap reconstruction performed under general anesthesia.Methods Two hundred and sixteen ASA Ⅰ-Ⅲ patients aged 18-80 yr undergoing vascular free flap reconstruction surgery were enrolled in this study.Patient characteristics before and during operation were recorded.The patients were followed up for 5 days after operation.Their level of consciousness,severity of pain and sleep quality were evaluated daily.The patients were divided into 2 groups according to the occurrence of delirium during the 5 days after operation:delirium group and non-delirium group.The method of CAM-ICU was reed in the diagnosis of postoperative delirium.Multivariate logistic regression was used to analyze the risk factors for postoperative delirium.Results logistic regression analysis showed that old age,history of alcohol abuse and sleep diacrder after operation were risk factors for delirium developed after free flap surgery.Conclusion Old age,history of alcohol abuse and sleep disorder after operation were the risk factors for postoperative delirium in patients after vascular free flap reconstruction performed under general anesthesia.     

PDGF与IGF对骨形成的协同作用及机制

骨的形成、发生、发展是一个极其复杂的过程,在众多的影响因素中,生长因子的局部调节发挥着重要的作用[1].同时,在任何时候,骨细胞的微环境中都存在不止一种生长因子,骨的形成是在多种生长因子相互作用的网络调节下实现的.因此研究骨生长因子之间的相互作用,对进一步阐明骨形成、发生机理,筛选有协同作用的生长因子联合应用修复骨缺损,治疗骨组织疾病,具有重要的理论和实践意义.现就PDGF与IGF的协同作用作以下综述.……