对叶百部中的非生物碱类成分

从百部科植物对叶百部中分离鉴定了11个化合物,分别为大黄素甲醚(1),3-羟基-4-甲氧基苯甲酸(2),对羟基苯甲酸(3),(Z)-1,1′-biindenyliden(4),胸腺嘧啶(5),2-(1′,2′,3′,4′-四羟基丁基)-6-(2′′,3′′,4′′-三羟基丁基)-吡嗪(6),掌叶半夏碱戊(7),豆甾醇(8),β-谷甾醇棕榈酸酯(9),β-谷甾醇(10)和β-胡萝卜苷(11)。其中化合物1～9均为首次从该科植物中分得。     

毛郁金化学成分的分离与鉴定

目的对毛郁金的化学成分进行研究。方法采用硅胶柱色谱、氧化铝柱色谱、ODS柱色谱和制备高效液相色谱等方法分离纯化毛郁金的化学成分,通过波谱数据分析确定化合物的结构。结果从毛郁金乙酸乙酯萃取物中分离得到10个化合物,分别鉴定为莪术二酮(curdione,1)、新莪术二酮(neocurdione,2)、吉马酮-4,5-环氧化物(germacrone-4,5-epoxide,3)、莪术二酮-1,10-环氧化物(curdione-1,10-epoxide,4)、gajutsulactone A(5)、莪术双环烯酮(curcumenone,6)、polydactin B(7)、4,5-seco-4,5-dioxo-guaia-1(10),11-diene(8)、2-endo-hydroxy-1,8-cineole(9)、邻苯二甲酸二丁酯(dibutylphthalate,10)。结论化合物3、5、7-10为首次从该植物中分离得到,其中化合物7-10为首次从姜黄属植物中分离得到。     

水飞蓟果实中的黄酮类化合物

从菊科水飞蓟属植物水飞蓟(Silybum marianum L.Gaertn)的干燥成熟果实中分离鉴定了9个化合物,分别为黄酮木质素类化合物:水飞蓟宾(1),2,3-脱氢水飞蓟宾(2),水飞蓟亭(3),2,3-脱氢水飞蓟亭(4);黄酮类化合物:4’-甲氧基紫杉叶素(5),紫杉叶素(6),二氢山奈酚(7),槲皮素(8)以及酚酸类化合物:3,4-二羟基苯甲酸(9)。其中化合物5,7,9为首次从该属植物中分得。     

桂枝的化学成分研究

目的研究中药桂枝(Ramulus Cinnamomi)的化学成分。方法利用硅胶柱色谱、Sephadex LH-20柱色谱及Pe-HPLC等方法进行分离纯化,根据理化性质及波谱分析鉴定化合物的结构。结果分离得到11个已知化合物,分别鉴定为反式桂皮酸(1)、花旗松素(2)、原儿茶酸(3)、反式-邻羟基桂皮酸(4)、原儿茶醛(5)、苯甲酸(6)、反式-邻甲氧基桂皮酸(7)、顺式-邻甲氧基桂皮酸(8)、对羟基苯甲酸(9)、香豆素(10)、胡萝卜苷(11)。结论化合物5为首次从该属植物中分离得到,化合物2、4、6、8、9首次从该植物中分离得到。     

温郁金挥发油的化学成分

目的对温郁金(Curcuma wenyujin)挥发油的化学成分进行研究。方法采用硅胶柱色谱、正反相MPLC及HPLC等进行分离纯化,通过波谱分析鉴定其结构。结果从温郁金挥发油中分离得到10个化合物,鉴定为莪术醇(1)、莪术二酮(2)、(4S,5S)-germacrone-4,5-epoxide(3)、germa-crone-1,10-epoxide(4)、新莪术二酮(5)、(5R,6R,7αR)-5-isopropenyl-3,6-dimethyl-6-vinyl-5,6,7α-tetrahydro-4H-enzofuran-2-one(6)、hydroxyisogermafurenolide(7)、(5R,6R,7aS)-5-isopropenyl-3,6-dimethyl-6-vinyl-5,6,7,7-αtetrahydro-4-Hbenzo-furan-2-one(8)、脱-氢1,8-桉叶素(9)、p-menth-2-ene-1,8-diol(10)。并通过核磁共振手段对其碳氢信号进行了全归属。结论化合物6、7为新的天然产物;化合物4、8、10为首次从该属植物中分离得到;化合物4、6、7、8、10为首次从该种植物中分离得到。     

狭叶荨麻化学成分研究

目的研究狭叶荨麻的化学成分。方法用薄层色谱、硅胶柱色谱、Sephadex LH-20、HPLC等色谱技术对狭叶荨麻地上部分95%乙醇提取物的化学成分进行了研究。结果从狭叶荨麻地上部分乙醇提取物的乙酸乙酯及正丁醇部分中分离得到了15个化合物,分别为5-羟甲基糠醛(1)、对羟基苯甲醛(2)、反式-4-羟基桂皮酸(3)、对羟基苯甲酸(4)、4-甲氧基苯甲酸(5)、白蜡树内酯(6)、东莨菪素(7)、正丁基-β-D-呋喃果糖苷(8)、苯甲酸(9)、赤藓醇(10)、β-谷甾醇(11)、胡萝卜苷(12)、刺槐素-7-O-β-葡萄糖苷(13),3,4-二羟基苯甲酸(14)、4-甲氧基咖啡酸(15)。结论其中化合物13、15为首次从荨麻属植物中分离得到,化合物2、4、14为首次从狭叶荨麻中分离得到。     

双边栝楼果实化学成分研究

目的研究双边栝楼果实化学成分。方法采用硅胶、sephadex LH-20、C-18反相柱层析以及重结晶等方法。结果从该植物中分离得到9个化合物,分别为黑麦草内酯(1)、7,22-二烯豆甾醇(2)、金圣草黄素(3)、邻羟基苯甲酸(4)、菠菜甾醇-7-O-葡萄糖苷(5)、金圣草黄素-7-O-葡萄糖苷(6)、香草酸(7)、对羟基苯甲酸(8)、异香草酸(9)。结论化合物1~9均为首次从该植物中分离得到。     

藏药狭叶红景天化学成分研究(英文)

研究藏药狭叶红景天的化学成分,应用硅胶、ODS和Sephadex LH-20凝胶柱色谱以及反相HPLC等多种技术进行分离和纯化,应用波谱技术确定化合物的结构。从藏药狭叶红景天乙醇提取物中分离鉴定了11个化合物,其中4-羟基-苯乙基-(4′-甲氧基-苯乙基)醚(1)和β-D-葡萄糖苷-2-羟基-2-甲基-丁酸酯(2)为新化合物;对乙氧基苯乙醇乙酯(3),对羟基苯甲酸乙酯(5),对羟基苯甲醛(7),R(-)-mellein(8),对甲氧基苯乙醇(10)5个化合物为首次从红景天属植物中得到,对羟基苯乙酮(4),对羟基苯甲酸(6),豆甾醇(9),3,4,5-三羟基苯甲酸甲酯(11)4个化合物为首次从狭叶红景天中得到。     

红火麻化学成分的分离与鉴定

目的研究蝎子草属植物红火麻的化学成分。方法利用各种色谱法进行分离纯化,通过理化性质和波谱数据分析进行结构鉴定。结果从红火麻中分离并鉴定了10个化合物,分别为金色酰胺醇乙酸酯(aurantiamide acetate,1)、齐墩果酸(oleanolic acid,2)、2α,3α,19α-三羟基-12-烯-28-乌苏酸(2α,3α,19α-trihydroxyurs-12-en-28-oic,3)、东莨菪素(scopoletin,4)、3-甲氧基-4-羟基苯甲酸(3-methoxy-4-hydroxybenzoic acid,5)、对羟基苯甲酸(p-hydroxybenzoic acid,6)、β-谷甾醇(β-si-tosterol,7)、胡萝卜苷(daucosterol,8)、5-羟甲基糠醛(5-hydroxymethyl furaldehyde,9)、甘露糖(mannose,10)。结论化合物1~10均为首次从红火麻中分离得到,化合物1~6、8~10为首次从蝎子草属植物中分离得到。     

灯盏花的化学成分研究

自灯盏花 [Erigeronbreviscapus(Vant.)Hand . Mazz .]全草乙醇提取物中分离得到 7个化合物 ,经理化性质和光谱分析分别鉴定为 :芹菜素 (1) ;山奈酚 (2 ) ;对羟基苯甲酸 (3) ;3 ,4 二羟基苯甲酸 (4 ) ;槲皮素 (5 ) ;木犀草素 (6 ) ;4′ 羟基黄芩素 (7)。其中山奈酚和槲皮素是首次从该植物中分离得到。     

Secoiridoid glycosides from the leaves of <Emphasis Type="Italic">Hydrangea macrophylla</Emphasis> subsp. <Emphasis Type="Italic">serrata</Emphasis>

Seven secoiridoid glycosides, secologanin dimethyl acetal (1), n-butyl vogeloside (2), n-butyl epi-vogeloside (3), (7R)-n-butyl morroniside (4), hydrangenoside A (5), hydrangenoside C (6), and hydrangenoside A dimethyl acetal (7), have been isolated from the leaves of Hydrangea macrophylla subsp. serrata (Thunb.) Makino (Saxifragaceae). The structures were elucidated on the basis of spectral data.     

A new ceramide from <Emphasis Type="Italic">Ramaria botrytis</Emphasis> (Pers.) Ricken

A new ceramide, (2S,2′R,3R,4E,8E)-N-2′-hydroxyoctadecanoyl-2-amino-9-methyl-4,8-heptadecadiene-1,3-diol (1), was isolated together with four known sterols, 5α,6α-epoxy-3β-hydroxy-(22E)-ergosta-8(14),22-dien-7-one (2), ergosterol peroxide (3), cerevisterol (4) and 9α-hydroxycerevisterol (5), from the fruiting bodies of Ramaria botrytis (Pers.) Ricken (Ramariaceae). The structure of the new compound was elucidated based on spectral data.     

Constituents of the roots of <Emphasis Type="Italic">Ligularia dentata</Emphasis> Hara

Nine known compounds, butyrospermol (1), lupeol (2), stigmast-4-en-3-one (3), stigmast-4-ene-3,6-dione (4), (+)-methyl abscisate (5), methyl indole-3-carboxylate (6), vanillin (7), methyl (E)-caffeate (8), and methyl (E)-ferulate (9), and two new compounds, methyl (E)-feruloylglycolate (10), and methyl-7,8-dihydro-(S)-7-methoxyferulate (11), have been isolated from the roots of Ligularia dentata Hara (Compositae). The structures were elucidated on the basis of spectral data.     

Cytotoxic activity of Perilla frutescens var. japonica leaf extract is due to high concentrations of oleanolic and ursolic acids

Three triterpene acids, ursolic acid (1), corosolic acid (2), and oleanolic acid (7), were isolated from the ethanol extract of leaves of Perilla frutescens (L.) Britton var. japonica Hara (Labiatae; Japanese name: Egoma). These acids are the active principles responsible for the cytotoxicity against three cultured human tumor cell lines, HL-60 (leukemia carcinoma), MCF-7 (breast carcinoma), and Hep-G2 (hepatic carcinoma), with half maximal inhibitory concentration (IC₅₀) values of 12–48 μM.     

Triterpenoic acids of Prunella vulgaris var. lilacina and their cytotoxic activities In Vitro

The column chromatographic separation of the MeOH extract from the aerial parts of Prunella vulgaris var. lilacina Nakai led to the isolation of fifteen triterpenoic acids (2–6, 9–13, 16–20), four flavonoids (14, 21–23), four phenolics (7, 8, 15, 24), and a diterpene (1). Their structures were determined by spectroscopic methods to be trans-phytol (1), oleanic acid (2) ursolic acid (3), 2α,3α,19α-trihydroxyurs-12en-28oic acid (4), 2α,3α-dihydroxyurs-12en-28oic acid (5), maslinic acid (6), caffeic acid (7), phydroxy cinnamic acid (8), 2α,3α,19α,23-tetrahydroxyurs-12en-28oic acid (9), 2α,3α,23-trihydroxyurs-12en-28oic acid (10), 2α,3β-dihydroxyurs-12en-28oic acid (11), 2α,3β,24-trihydroxyolea-12en-28oic acid (12), (12R, 13S)-2α,3α,24,trihydroxy-12,13-cyclo-taraxer-14-en-28oic acid (13), quercertin 3-O-β-D-glucopyranoside (14), rosmarinic acid (15), 2α,3α,24-trihydroxyurs-12,20(30)-dien-28oic acid (16), 2α,3α,24-trihydroxyolea-12en-28oic acid (17), 2α,3β,19α,24-tetrahydroxyurs-12en-28oic acid 28-O-Dglucopyranoside (18), 2α,3α,19α,24-tetrahydroxyurs-12en-28oic acid 28-O-D-glucopyranoside (19), prunvuloside A (20), kaempferol 3-O-α-L-rhamnopyranosyl(1→6)-β-D-glucopranoside (21), kaempferol 3-O-β-D-glucopyranoside (22), quercertin 3-O-α-L-rhamnopyranosyl(1→6)-β-D-glucopyranoside (23), and 2-hydroxy-3-(3’,4’-dihydroxyphenly)propanoic acid (24). Compounds 1, 8–12, 17, 21, 23, and 24 were isolated from this plant source for the first time. The isolated compounds were evaluated for their cytotoxicity against A549, SK-OV-3, SK-MEL-2, and HCT15 cells in vitro using the sulforhodamin B bioassay (SRB) method. Compound 3 exhibited moderate cytotoxic activity against A549, SK-OV-3, SK-MEL-2, and HCT15 cells, with ED₅₀ values of 3.71, 3.65, 13.62, and 5.44 μM, respectively.     

A new kaempferol trioside from <Emphasis Type="Italic">Solidago altissima</Emphasis> L.

A new kaempferol trioside [kaempferol 3-O-rutinoside 7-O-β-d-apiofuranoside, (1)] was isolated together with nine phenolic compounds, trans-tiliroside (2), caffeic acid (3), chlorogenic acid (4), 3-O-caffeoylquinic acid (5), 4-O-caffeoylquinic acid (6), 3-O-coumaroylquinic acid (7), 4-O-coumaroylquinic acid (8), 3,5-di-O-caffeoylquinic acid (9) and 4,5-dicaffeoylquinic acid (10) from the leaves of Solidago altissima L. The structure elucidations of the compounds were based on the analyses of spectroscopic data.     

In vitro antimalarial activity of prenylated flavonoids from Erythrina fusca

Ethyl acetate (EtOAc) extract from the stem bark of Erythrina fusca showed a antimalarial activity against the multi-drug-resistant strain (K1) of Plasmodium falciparum, and six flavonoids, lupinifolin (1), citflavanone (2), erythrisenegalone (3), lonchocarpol A (4), liquiritigenin (5), and 8-prenyldaidzein (6), were isolated from the extract. Diprenylated flavanone 4 showed a notable antimalarial activity (IC₅₀; 1.6 μg/mL); however 1 and 3 did not show the activity, even though these compounds possessed prenylated substitution.     

New prenylated flavones from <Emphasis Type="Italic">Artocarpus champeden</Emphasis>, and their antimalarial activity in vitro

Two new prenylated flavones, artocarpones A and B (1 and 2), and seven known isoprenylated flavonoids, artonin A (3), cycloheterophyllin (4), artoindonesianin E (5), artoindonesianin R (6), heterophyllin (7), heteroflavanone C (8), and artoindonesianin A-2 (9), have been isolated from the stem bark of Artocarpus champeden. Their structures were determined by spectroscopic analysis. Among the compounds isolated, 8 had the most potent inhibitory activity against the growth of Plasmodium falciparum 3D7 clone, with an IC₅₀ value of 1 nmol L⁻¹.     

Stilbenes and oligostilbenes from leaf and stem of <Emphasis Type="Italic">Vitis amurensis</Emphasis> and their cytotoxic activity

Chromatographic separation of the EtOAc fraction from the leaf and stem of Vitis amurensis led to the isolation of six oligostilbenoids (i.e., r-2-viniferin (1), trans-amurensin B (2), trans-ɛ-viniferin (3), gnetin H (4), amurensin G (5), (+)-ampelopsin A (8)) and four stilbenoids (i.e., trans-resveratrol (6), (+)-ampelopsin F (7), piceatannol (9), and trans-piceid (10)). The structures have been identified on the basis of spectroscopic evidence and physicochemical properties. The isolates were investigated for cytotoxic activity against three cancer cell lines in vitro using the MTT assay method. Amurensin G (5) and trans-resveratrol (6) showed significant cytotoxic activity against L1210, K562 and HTC116 cancer cell lines with IC₅₀ values ranging from 15.7 ± 2.1 to 30.9 ± 1.8 μM. (+)-Ampelopsin A (8) and trans-piceid (10) exhibited considerable cytotoxic activity against L1210 (IC₅₀ values of 30.6 ± 4.1 and 28.7 ± 2.81 μM, respectively) and K562 (IC₅₀ values of 38.6 ± 0.82 and 24.6 ± 0.76 μM, respectively). Gnetin H (4) showed only weak cytotoxic activity against L1210 with an IC₅₀ value of 40.1 ± 4.23 μM. On the other hand, r-2-viniverin (1), trans-amurensin B (2), trans-ɛ-viniferin (3), (+)-ampelopsin F (7), and piceatannol (9) exhibited no activity on three cancer cell lines.     

New isoflavones from Belamcandae Rhizoma

Four new isoflavones (iristectrigenin A-7-glucoside (2), 8-hydroxytectrigenin (5), 8-hydroxyiristectrigenin A (6), and 8-hydroxyirigenin (7–1) have been isolated from Belamcandae Rhizoma (the rhizome of Belamcanda chinensis), along with nine known compounds: tectridin (1), tectrigenin-4′-glucoside (3), astragalin (4), irilin D (7–2), isotectrigenin (8), tectrigenin (9), iristectrigenin B (10), hispiludin (11–1), and irigenin (11–2). The structures of 2, 5, 6 and 7–1 were determined by spectroscopic methods, including high-resolution mass spectrometry (HR-MS), proton nuclear magnetic resonance (¹H-NMR), carbon-13 NMR (¹³C-NMR), and various two-dimensional (2D)-NMR techniques.