ANNULATE LAMELLAE OBSERVED IN HUMAN PROSTATIC CARCINOMA

Annulate lamellae (AL) were observed in only three out of 40 cases of human prostatic carcinoma, but not in 20 cases of benign prostatic hyperplasia and 11 cases of presumably normal prostatic tissues. AL showed the continuity with the rough endoplasmic reticulum and seemingly the nuclear membrane consisting of lamellar or concentric arrangement of stacked membranes and occasionally annular structures. In addition, annuli were detected in the rough endoplasmic reticulum near the stacked membranes which were devoid of ribosomal attachment. These results disclosed that in human prostatic tissues, AL could be only rarely detected in actively dividing cancer cells, and were seemingly the temporary transitional structures transforming from the nuclear membrane to the rough endoplasmic reticulum.     

ANNULATE LAMELLAE IN GASTRIC CARCINOMA

A 72-year-old male painter, who complained of his "lungs burning" for 2 weeks, died suddenly. Autopsy examination revealed severe coronary atherosclerosis with plaque rupture as the cause of death. Examination of the lungs revealed emphysema, interstitial fibrosis, and multinucleated giant cells with intra- and extracellular brown-black, crystalline, polarizable foreign material. Energy-dispersive X-ray microanalysis showed the material to contain titanium, aluminum, silicon, and iron. An increased incidence of respiratory disease has been reported in professional painters. Titanium is widely used as a pigment in the manufacturing of commercial paints. Cases of pneumoconiosis and alveolar proteinosis have been described in painters in which analysis of lung tissue revealed increased levels of titanium. This case is presented as an example of a rarely reported phenomenon, which may have clinical implications for evaluation and management of lung disease in painters.     

AN IMMUNOHISTOCHEMICAL STUDY OF NEOVASCULATURE IN HUMAN BRAIN TUMORS

In order to elucidate the characteristics of newly-formed small blood vessels in brain tumors, 51 cases of various types of brain tumor were studied immuno-histochemically. Endothelial cells in all tumor types reacted positively with anti-Factor VIH-related antigen (F-VIII Ag) and UEA-1. Reactivity to UEA-1 was limited to endothelial cytoplasm. Proliferating immature endothelial cells showed weak reactivity to anti-F-VIII Ag. In some cases of benign tumors diffuse reactivity to anti-F-VIII Ag was noted throughout thickened medial areas of the vascular walls, in which reactivity to anti-fibrous collagen and anti-flbronectin was noted. UEA-1 was thought to be a more specific endothelial marker than F-VIII Ag, especially for proliferating and Immature endothelial cells. It is strongly suspected that fibrous collagens are related to the formation of a thickened vascular wall in newly-formed small tumor vessels.     

AN ULTRASTRUCTURAL STUDY ON PERIDUCTAL ELASTOSIS IN HUMAN BREAST TUMORS

An ultrastructural study on elastosis of human breast tumors was made with special attention to the periductal elastosis and the cell responsible for elastic fiber formation. The elastosis was found prominently in scirrhous type of duct carcinoma. In the area of mild periductal elastosis, the elastic fibers with many microfibrils and a tiny central elastin were seen around the periductal fibroblasts which were characterized by attenuated cytoplasms with aggregates of microfilaments and slightly developed rough endoplasmic reticulum. With the thickening of the periductal wall, such an area was replaced by abundant mature elastic fibers with peripheral microfibrils and a few intervening ordinary fibroblasts. Therefore, it was suggested that the periductal fibroblasts which transformed into ordinary fibroblasts during the development of elastosis were primarily concerned with the elastic fiber formation. In the interlobular tissue in which both fibroblasts and myofibroblasts were present, the elastic fibers were larger than those of the periductal area and had less microfibrils in their periphery. The relationship between microfibrils and elastin during the early elastosis, maturation process of the elastic fibers, and cell modulation of the fibroblasts in the breast elastosis were discussed.     

INTRANUCLEAR MEMBRANOUS PROFILES IN GERMINOMA CELLS——A VARIANT OF NUCLEAR POCKETS and INTRANUCLEAR ANNULATE LAMELLAE

When ultrastructurally examining 24 germinomas comprising 12 seminomas, 4 dysgerminomas, 1 mediastinal germinoma, and 7 intracranial germinomas, intranuclear membranous profiles were noticed in 17 germinomas, ranging from 20-100 nm in width and 3^m in length. With occasional connections to the nuclear envelope through a small hole, intranuclear membranous profiles in germinoma cells were considered as clefts of the nuclear envelope. While most frequently situated under the inner nuclear membrane, they varied in configuration as well as distribution. As sequestered round mass of nuclear material, they were a variant of nuclear pockets containing nucleoplasm. Intranuclear annulate lamellae were occasionally present apart from the nuclear envelope and connected with nuclear clefts. Eleven of the twelve seminomas and 6 of the twelve non-seminomatous germinomas showed intranuclear membranous profiles, and the incidence of such profiles was much higher in seminomas than in non-seminomatous germinomas. Intranuclear membranous profiles facing the inner nuclear membrane were also noted in spermatogonia in adolescents and adults. It was suggested that intranuclear membranous profiles in germinoma cells could be structures following ones occasionally seen in spermatogonia. ACTA PATHOL. JPN. 35: 605–619, 1985.     

乳腺瘤组织内纤维连结蛋白分布的变化

纤维连结蛋白(Fibronectin Fn)作为组织细胞表面的一种大分子糖蛋白,在细胞与细胞之间、细胞与周围基质之间起着连结作用。我们曾观察过Fn在正常人体组织内的分布,但它是否可作为组织细胞恶变的标志,目前尚无定论。最近Colli在研究了恶性和良性肿瘤导致的腹水中的众多指标之后提出,Fn在鉴别恶性与良性腹水时是最有意义的指标。而Stenman对73例实体瘤组     

肿瘤组织中端粒酶活性的研究

目的 了解端粒酶活性高低与肿瘤恶性程度关系。方法 用ＴＲＡＰ－ＥＬＩＳＡ和我们改进的ＴＲＡＰ银染,对肿瘤组织酶活笥进行半定量检测。结果 端粒酶阳性率分别为：胶质瘤星形Ⅱ级４０％,Ⅲ和Ⅳ级１００％,肠癌８４．６２％,食管癌９０％。结论 端粒酶活性与肿瘤恶性程度密切相关,是诊断恶性肿瘤、判断愈后的良好指标。     

FINE STRUCTURE OF CYTOPLASMIC PARACRYSTALLINE IN HUMAN HEPATIC PARENCHYMAL CELLS

Cytoplasmic paracrystalline structures were observed in the hepatic parenchymal cells from a chronic alcoholic and non-alcoholic patients. The structure consisted of parallel filaments 8 to 10 m/< thick and 15 m/< apart crossing each other at an approximate angle of 60^o and forming lattice-like structure. Such non-membrane bound paracrystalline was mainly located near the nucleus and was estimated to correspond to the masses positively stained with PAS reaction. It may be probable that the cytoplasmic paracrystalline is a newly synthesized product or a stored substance.     

INDUCTION OF INTRACRANIAL TUMORS IN MICE BY HUMAN ADENOVIRUS TYPE 12

C₃Hf/Bi mice inoculated intracranially with human adenovirus type 12 were studied by fluorescent antibody technique and light microscopy.
The cells with fluorescent T antigens were present in the wall of the ventricles and in the epithelium of the choroid plexuses. Tumors were induced in 21 of 45 mice and the predilection sites were the olfactory bulb, the floor of the fourth ventricle and the tapetum which was in contact with the hippocampus.
The relationship between production of the T antigen and tumor development as well as histological features of the tumors was discussed.     

PRODUCTION OF ALPHA-FETOPROTEIN BY HUMAN GERM CELL TUMORS IN VIVO AND IN VITRO

The production of alpha-fetoprotein (AFP) by human germ cell tumors was studied in surgical specimens, cultured cells and transplanted tumors in nude mice. AFP was detected most frequently in yolk sac (endodermal sinus) tumors, and it was also detected, though only occasionally, in embryonal carcinomas and in teratomas. AFP-positive cells in teratomas presented a glandular or hepatoid appearanece. Our experimental data suggest that AFP production in embryonal carcinomas is due to the functional expression of yolk sac differentiation preceding its morphologic expression. Although the transplanted tumors maintained the productivity of AFP, the cultured cells were not successful in maintaing a continuous AFP production.     

人小脑皮质颗粒细胞的超微结构

本文用透射电镜观察了人小脑颗粒细胞的超微结构。在人的颗粒层中存在少量髓化的颗粒细胞。它们中有的被髓鞘完全包绕,有的被部分包绕,可以观察到有的髓化颗粒细胞的髓鞘来自二个小的有髓纤维。还观察到极少的颗粒细胞具有典型的核仁。     

幕上肿瘤的短潜伏期体感诱发电位研究

本研究测定了116例幕上肿瘤的短潜伏期体感诱发电位(SLSEP),其中包括神经胶质瘤、脑膜瘤、神经鞘瘤、转移瘤等。在幕上肿瘤116例中,98例经术后病理学检查证实。结果表明:幕上肿瘤SLSEP总异常率为81.0％,其中位于额顶叶或半球深部者异常率较高。作者还观察到,幕上恶性肿瘤的SLSEP异常率比良性高。研究结果提示:SLSEP的测定对幕上肿瘤的定位和定性诊断有较高的应用价值。     

FINE STRUCTURAL HISTOCHEMISTRY OF MYOSIN-ATPASE ACTIVITY IN THE SKELETAL MUSCLES OF DUCHENNE MUSCULAR DYSTROPHY

Myosin ATPase activity was fine-structurally examined in various skeletal muscle lesions including atrophy, degenerations, necrosis, "deltashaped subsarcolemmal lesion", and apparently normal fibers from seven patients of Duchenne muscular dystrophy (D M D). The enzyme activity was almost completely lost in the foci of necosis, and more or less markedly diminished in various kinds of degenerations, while it was well preserved in the apparently normal and simply atrophic fibers. The results suggested that there were two different lesions in wasting of the skeletal muscles in D M D; necrotic process with degenerations in which myosin-ATPase activity was affected and simply atrophic process which had little influence on the enzyme activity. The former lesion might be related with direct or indirect damages such as proteolysis, while the latter seemed to be an expression of basically different process such as disuse or denervation, or a more mild expression basically of the same process ultimately resulting in necrosis.     

人体步行运动的关节力学研究

利用角度传感器和步态测力板,本文观察了人体的步行运动,采用Fourier数字滤波拟合出各关节的运动规律,依据多刚体动力变分原理建立了适合作实时计算和模型修改的人体步行动力学方程。对于步行中双脚着地时出现的闭环结构,作者借助测力板测出的脚底反力曲线,提出了由力——位移组成的混合求解法。用文中计算方法编制的软件可用于人体下肢疾病的临床医学诊断。     

FINE STRUCTURAL COMPARISON OF EWINGS SARCOMA WITH NEUROBLASTOMA

Two cases of Ewing's sarcoma and two of neuroblastoma, rosette forming and round cell type, were studied electron microscopically and their fine structures were compared. The neoplastic cells of Ewing's sarcoma were characterized by aggregated glycogen particles in the cytoplasm. They had pseudopod-like cytoplasmic processes having tight junctions, which never contained microtubules or mitochondria. Ewing's sarcoma cells exhibited several stages of cell maturation and some mature cells possessed a large amount of smooth and rough endoplasmic reticulum, large Golgi complexes and numerous phagosomes containing glycogen particles as well as cytoplasmic organelles. The neoplastic cells of neuroblastoma, rosette forming type, were characterized by synaptic junctions and numerous cytoplasmic processes with production of neurites containing microtubules, neurofibrils, mitochondria and a few catecholamine granules. A few cytoplasmic processes containing mitochondria were observed even in the round cell type.     

深圳人群弓形虫感染的血清流行病学调查研究

为了调查目前深圳地区人群弓形虫感染情况 ,本文自 1997年至 1999年采用酶联免疫吸附试验( ELISA)对深圳地区不同人群 12 2 0份血清进行检查 ,结果总人群 Ig G阳性率为 11.15% ,Ig M阳性率为0 .2 5%。其中 ,幼儿组、青少年组、成年组、老年组 Ig G阳性率分别为 2 .33%、 5.68%、 8.90 %、 10 .2 3% ;动物饲养员、屠宰工人的阳性率为 2 3.57%、 2 5.0 0 % ,与阳性率分别为 14 .66%、 7.14 %、 6.32 %、 5.68%的饮食业人员、目前没有与动物接触职业者、医务工作者、中学生之间有显著性差异 ( P<0 .0 0 5) ;男性 Ig G阳性率为 11.73% ,与 Ig G阳性率为 10 .66%的女性比较无差异 ( P>0 .5)。结果显示深圳地区人群感染率有随年龄递增而升高趋势 ,且不同职业之间亦有显著差异性 ,但性别差异无显著性。     

人胚胎期胰腺的形态学研究

本研究收集胎龄5～41周的人胚胎53例,取胰腺,Bouin液固定,按常规制石蜡切片。部分切片经阿利新蓝-焰红、硝酸银-醛复红-铅苏木精等方法染色,部分切片作免疫组织化学PAP反应,观察胰岛A、B和D细胞。取不同胎龄的胰尾组织14例,戊二醛固定,Epon812包埋,透射电镜观察。本文的结果表明,胰腺内分泌部主要是由胰管2级以下分支的管壁上皮细胞,向周围的间充质内增生和分化而成。胎龄12周时,即可在光镜下辨认A、B及D细胞;电镜下观察,胰腺外分泌细胞在胎龄14周以后,才能见到分泌颗粒,证明胰岛细胞的分化早于外分泌细胞。测量16～36周胎儿胰腺内胰岛所占的比例,证明胚胎期胰腺内分泌部所占比例远高于成年人。本实验为临床使用人胎胰岛移植提供了参考资料。     

成人房室隔的研究

利用56例成人心脏标本和4例心脏切片对人心房室隔进行了观察和测量.认为房室隔位于右房与左室之间,上界为二尖瓣前瓣环和主动脉后瓣环与右瓣环,下界为三尖瓣隔侧瓣附着缘.前界为室上嵴后缘,后界为冠状窦口.房室隔分为前部与后部,分别与左室流出道和流入道相对应.测量了房室隔的下缘下、前部长和后部长,分别为2.95±0.08,1.62±0.07和1.33±0.07cm;测量后缘宽、中部宽和前部宽,分别为1.61±0.05,1.04±0.04和0.49±0.04cm.描述了各部的组织结构.对房室隔的命名、机能和临床意义进行了讨论.