CRP、SAA、NLR联合检测在HIV/AIDS相关机会性感染中的诊断价值

目的 探讨C-反应蛋白(C-reactive protein, CRP)与血清淀粉样蛋白A(serum amyloid A,SAA)联合中性粒细胞/淋巴细胞比值(neutrophil to lymphocyte ratio, NLR)对HIV/AIDS相关机会性感染的诊断价值。方法 选取2021年1月至2022年6月收治的HIV/AIDS机会性感染患者150例,HIV/AIDS无机会性感染患者120例,依据不同病原学将150例机会性感染患者分为细菌感染组、病毒感染组、真菌感染组与混合感染组,检测CRP、SAA、WBC计数,计算中性粒细胞/淋巴细胞比值(NLR),采用受试者工作特征(receiver operating characteristic, ROC)曲线分析CRP、SAA、SAA/CRP比值、WBC计数、NLR在不同感染中的诊断效能。结果 机会性感染组CRP、SAA、SAA/CRP比值、NLR均高于无机会性感染组,差异有统计学意义(P<0.05),WBC计数差异无统计学意义(P>0.05);机会性感染组中Ⅲ期SAA、SAA/CRP比值、NLR均高于I期与II期,差异...     

SAA、CRP 联合血常规检测在儿童急性呼吸道感染中的临床应用价值

目的:研究血清淀粉样蛋白A(Serum amyloid A protein,SAA)、C反应蛋白(C reactive protein,CRP)联合血常规检测在儿童急性呼吸道感染中的意义.方法:回顾性分析2019年4月至2020年4月我院收治的424例感染性疾病患儿(细菌感染组133例,病毒感染组167例及支原体感染组124例)及同期本院体检的健康受检儿150例(对照组).比较不同组白细胞(White blood cell,WBC)、CRP及SAA水平及不同检测方法的诊断效能.结果:细菌感染组患儿CRP、WBC以及SAA水平均高于对照组;CRP、WBC水平高于病毒感染以及支原体感染组;SAA水平低于病毒感染、支原体感染组;病毒感染、支原体感染组CRP、SAA水平高于对照组(P<0.05).CRP、WBC、SAA联合检测特异度、敏感度、AUC均高于单一检测法(P<0.05).结论:SAA、CRP及WBC联合检测有利于提高儿童急性上呼吸道感染的诊断准确率.     

探讨C反应蛋白在儿童急性呼吸道感染中的应用价值

目的 探讨C反应蛋白(CRP)在儿童呼吸道感染早期诊断和疗效判断中的价值.方法 检测急性呼吸道细菌感染组75例,病毒感染组70例,健康儿童60例CRP含量和白细胞计数.结果 与病毒感染组、对照组相比较,细菌感染组CRP明显升高(P<0.01),细菌感染组治疗前后CRP比较,差异有统计学意义(P<0.01).     

超敏C反应蛋白等指标在川崎病的动态变化和临床诊断价值

目的:观察川崎病(KD)患儿治疗前后全血超敏C反应蛋白(hs-CRP)、C反应蛋白(CRP)含量和白细胞计数(WBC)、血小板计数(PLT)指标变化,并探讨它们对KD的临床诊断价值。方法:37例KD患儿(KD组)和65例健康幼儿(正常对照组)采用常规方法平行检测hs-CRP、CRP、WBC和PLT水平,并分析各指标组间差异和KD患儿治疗前后变化。通过ROC曲线分析比较各指标对KD的临床诊断价值。结果:KD组治疗前hs-CRP、CRP、WBC和PLT均较正常对照组明显升高(P0.01);治疗后hs-CRP、CRP和WBC均较治疗前降低(P0.01),而PLT在治疗后升高(P0.01)。ROC曲线分析显示hs-CRP和CRP的cut-off值分别为0.51mg/L和5.50mg/L时,两者ROC曲线下面积(AUC)分别为0.964和0.957,敏感度分别为97.30%和91.92%,特异度分别是56.87%和95.40%,临床诊断价值明显优于WBC和PLT。结论:快速检测全血hs-CRP、CRP、WBC可用于KD动态监测;hs-CRP和CRP对KD有重要临床诊断价值。     

儿科早期预警评分及常见血常规、炎症标志物对危重症手足口病患儿早期诊断的价值

目的 探讨儿科早期预警评分(EWS)及常见血常规、炎症标志物对危重症手足口病(HFMD)患儿早期诊断的价值.方法 收集2016年6月至2020年6月我院儿科诊治的120例危重症HFMD患儿(疾病组)和普通型HFMD患儿60例(对照组)的临床资料,比较两组早期预警评分(EWS)、主要血常规检测结果 [白细胞计数(WBC)、降钙素原(PCT)和中性粒细胞比例(NEUT)]、炎症标志物[白介素-6(IL-6)、白介素-8(IL-8)、白介素-10(IL-10)、肿瘤坏死因子-α(TNF-α)和C反应蛋白(CRP)]水平.采用ROC曲线分析EWS及常见血常规、炎症标志物对危重症HFMD早期诊断价值.结果 疾病组EWS评分、WBC、PCT、NEUT较对照组明显升高(P<0.05),疾病组IL-6、IL-8、IL-10、TNF-α、CRP水平较对照组明显升高(P<0.05),ROC曲线分析提示EWS、常见血常规检查结果 、炎症标志物联合检测诊断危重症HFMD曲线下面积、灵敏度和特异性较三者分别检测的高(P<0.05),HFMD患儿EWS评分与WBC、PCT、NEUT、IL-6、IL-8、IL-10、TNF-α、CRP呈明显正相关(P<0.05).结论 儿科EWS、常见血常规检查结果 、炎症标志物联合检测可明显提高危重症H FMD早期诊断,有望为危重症H FMD的早期诊治提供理论依据.     

PCT和CRP联合检测在儿童流感、水痘和腮腺炎的临床价值

目的 探讨PCT和CRP联合检测对儿童流感、水痘和腮腺炎的临床价值.方法 筛选2013年6月至2015年6月在本院就诊的60例流行性感冒患儿(流感组)、50例水痘患儿(水痘组)、40例腮腺炎患儿(腮腺炎组)和30例正常体检儿童(对照组),检测血清C-反应蛋白(Creactive protein,CRP)、降钙素原(procalcitonin,PCT),比较流感组、水痘组和腮腺炎组的PCT和CRP的检测结果.采用Logistic回归分析获得新的统计量,计算各曲线下面积(AUC),探讨二者在流感、水痘和腮腺炎的诊断价值.结果 (1)水痘组和腮腺炎组的PCT、CRP高于流感组(P＜0.05),且三组中的PCT、CRP均高于对照组(P＜0.05).(2)以对照组儿童为诊断依据,根据ROC曲线“灵敏度+特异性”取最大值的原则,确定流行性感冒、水痘、腮腺炎患儿的PCT和CRP的最佳截点分别为0.25ng/mL、0.45 ng/mL和0.35ng/mL;6.85mg/L、8.05mg/L和4.7mg/L.(3)CRP+ PCT的ROC曲线下的面积(AUC)明显大于PCT和CRP任一的AUC.结论PCT和CRP在流感、水痘和腮腺炎的诊断中有一定的临床价值,二者联合检测诊断效价更佳.     

联合检测RBP、SAA和CRP对妊娠期高血压疾病肾损伤的诊断价值

目的 探究视黄醇结合蛋白(RBP)、血清淀粉样蛋白A(SAA)、C-反应蛋白(CRP)联合检测对妊娠期高血压疾病(HDP)肾损伤的诊断价值.方法 选取2018年12月至2019年12月来我院孕检门诊诊断为HDP患者120例为研究组,按照内生肌酐清除率(Ccr)分为肾功能正常组69例和肾功能损害组51例,选择同期来我院孕检门诊进行常规体检孕妇47例为对照组,检测入组对象的RBP、SAA、CRP水平,并运用受试者工作曲线(ROC曲线)进行HDP肾损伤诊断效能的分析.结果 研究组患者的RBP、SAA、CRP均显著性高于对照组(P<0.05),且研究组中肾功能损伤患者的RBP、SAA、CRP均升高的更明显(P<0.05);ROC曲线显示,RBP的诊断最佳临界值为31.673mg/L,灵敏度及特异性分别为66.7％和65.2％,约登指数为0.319,AUC为0.604;SAA的诊断最佳临界值为13.680mg/L,灵敏度及特异性分别为78.4％和88.4％,约登指数为0.668,AUC为0.889;CRP的诊断最佳临界值为5.508mg/L,灵敏度及特异性分别为74.5％和69.6％,约登指数为0.441,AUC为0.781;RBP、SAA两者联合检测灵敏度及特异性分别为64.7％和70.1％,约登指数为0.618,AUC为0.718;RBP、CRP两者联合检测灵敏度及特异性分别为76.5％和68.1％,约登指数为0.446,AUC为0.758;SAA、CRP两者联合检测灵敏度及特异性分别为88.2％和84.1％,约登指数为0.723,AUC为0.907;三者联合检测灵敏度及特异性分别为90.2％和89.4％,约登指数为0.656,AUC为0.974.结论 HDP患者的RBP、SAA、CRP水平明显升高,且HDP发生肾损伤的患者的RBP、SAA、CRP水平更高,三者联合检测对HDP肾损伤具有较高的诊断价值.     

CRP与WBC联检在儿科呼吸道感染性疾病诊断中的应用价值

目的：探讨CRP联合WBC检测在儿科急性呼吸道感染中的诊断价值。方法采集320例急性呼吸道感染患儿的血清,其中细菌感染组180例,病毒感染组70例,肺炎支原体感染组70例,检测其C-反应蛋白和白细胞值,并与100例健康儿童的血清检测资料进行比较。结果细菌感染组的CRP阳性率(90.00%)高于WBC阳性率(49.44%),MP感染组的CRP阳性率(11.43%)低于WBC阳性率(54.29%),两者比较有显著性差异(P<0.05);细菌感染组的CRP及WBC阳性率,MP感染组的WBC阳性率均高于病毒感染组和对照组,两组比较有显著性差异(P<0.05)。结论 CRP和WBC联检,对儿童急性呼吸道感染性疾病的病原体能够做出早期鉴别诊断且快速方便,成本低廉,值得临床推广应用。     

外周血CD64指数对创伤手术后脓毒症的早期诊断价值

目的探讨外周血CD64指数在创伤手术后脓毒症患者中的表达及早期诊断价值。方法以420例创伤患者为研究对象,按临床表现分为脓毒症组(130例)和普通组(290例)。采用流式细胞术检测外周血CD64,同时检测C-反应蛋白(CRP)及白细胞计数(WBC)水平。评价上述指标对创伤手术后脓毒症患者的早期诊断价值。结果脓毒症组患者外周血CD64指数及CRP、WBC水平明显高于普通组患者(P0.05);经过相应治疗后,脓毒症组患者外周血CD64指数、CRP及WBC水平均较治疗前显著降低(P0.05)。相关性分析显示,外周血CD64指数与CRP水平呈正相关(r=0.79,P0.01),而外周血CD64指数与WBC水平无相关性(r=0.028,P0.05)。ROC曲线分析显示,以外周血CD64指数7.21为早期诊断创伤手术后脓毒症临界值时,诊断创伤手术后脓毒症的敏感性和特异性分别为85.1%和87.8%,ROC曲线下面积(AUC)为0.887(95%CI 0.784~0.929),显著优于CRP指标。结论 CD64在创伤手术后脓毒症患者中高表达,其可作为早期诊断创伤手术后脓毒症的有效指标,具有一定的临床运用价值。     

流式细胞术检测中性粒细胞CD64指数在鉴别早期感染性疾病中的临床价值分析

目的 分析流式细胞术检测中性粒细胞CD64指数在鉴别早期感染性疾病中的临床价值.方法 选取我院2015年10月至2017年4月期间收治的感染性疾病患者200例,根据感染严重程度分为早期局部感染组(n=120)和脓毒血症组(n=80),另选同期入院体检的健康志愿者50例作为对照组.所有研究对象均于入院后24 ～ 48h内及治疗前进行血常规WBC检测和CRP测定,采用流式细胞仪多参数分析中性粒细胞CD64 (MESF)及中性粒细胞CD64指数,通过绘制受试者工作特征(ROC)曲线计算中性粒细胞CD64指数鉴别早期感染性疾病的灵敏度和特异性.结果 对照组、早期局部感染组、脓毒血症组中性粒细胞CD64及血清CRP、WBC水平依次显著升高,各组差异有统计学意义(P＜0.05).早期局部感染组中性粒细胞CD64指数与血浆CRP、WBC水平呈正相关(P＜0.05).中性粒细胞CD64指数、CRP、WBC的曲线下面积分别为0.896、0.757、0.629(P均＜0.05);与CRP、WBC相比,中性粒细胞CD64指数鉴别早期感染性疾病的灵敏度、特异性为97.39％、90.59％明显升高.结论 分析外周血中性粒细胞CD64指数对于早期感染性疾病的鉴别诊断有重要价值,其诊断效能明显优于血常规WBC和CRP检测.     

Role of adenosine A1 receptors in the modulation of dopamine D1 and adenosine A2A receptor signaling in the neostriatum

Adenosine is known to modulate the function of neostriatal neurons. Adenosine acting on A(2A) receptors increases the phosphorylation of dopamine- and cAMP-regulated phosphoprotein of M(r) 32 kDa (DARPP-32) at Thr34 (the cAMP-dependent protein kinase [PKA] site) in striatopallidal neurons, and opposes dopamine D2 receptor signaling. In contrast, the role of adenosine A(1) receptors in the regulation of dopamine/DARPP-32 signaling is not clearly understood. Here, we investigated the effect of adenosine A(1) receptors on D(1), D(2) and A(2A) receptor signaling using mouse neostriatal slices. An A(1) receptor agonist, 2-chloro-N(6)-cyclopentyladenosine (100 nM), caused a transient increase, followed by a transient decrease, in DARPP-32 Thr34 phosphorylation. Our data support the following model for the actions of the A(1) receptor agonist. The A(1) receptor-induced early increase in Thr34 phosphorylation was mediated by presynaptic inhibition of dopamine release, and the subsequent removal of tonic inhibition by D(2) receptors of A(2A) receptor/G(olf)/cAMP/PKA signaling. The A(1) receptor-induced late decrease in Thr34 phosphorylation was mediated by a postsynaptic G(i) mechanism, resulting in inhibition of D(1) and A(2A) receptor-coupled G(olf)/cAMP/PKA signaling in direct and indirect pathway neurons, respectively. In conclusion, A(1) receptors play a major modulatory role in dopamine and adenosine receptor signaling.     

A year in the life of the immunoglobulin superfamily

The superfamily of molecules with immunoglobulin-like domains has recently been gaining new members-largely on the basis of sequence homology. Here Alan Williams reviews this new work and reveals how the comparison of sequence patterns enables decisions on membership to be made. Accommodation of the new structures demands the provision of new categories, and forces the abandonment of the conserved disulphide bond as the last invariant characteristic of an immunoglobulin-type domain. They may, however, provide more dues to the origins and evolution of the immunoglobulin superfamily.     

A functional defect in the early phase of the immune response observed in patients with hemophilia A

In search of a functional immunological defect in patients with hemophilia A, we investigated the early phase of the immune response and found a deficiency in the monocyte-T cell interaction after in vitro exposure to a bacterial antigen. T-cell proliferation in response to Escherichia coli-pulsed autologous monocytes was significantly (P less than 0.025) reduced in hemophiliacs [mean dpm +/- SEM (n = 25): 9036 +/- 2600] as compared to healthy controls [mean dpm +/- SEM (n = 24): 17,812 +/- 2985]. This functional defect was expressed in a severe form (antigen-induced monocyte-T cell interaction less than or equal to 2000 dpm) in both HTLV III antibody-positive and -negative patient populations. Significantly decreased T-helper/T-suppressor-cell ratios (mean dpm +/- SEM: patients 1.47 +/- 0.13; controls 2.04 +/- 0.11; P less than 0.001)--but without a concomitant reduction in the absolute number of T-helper cells--could also be observed.     

A demonstration of the dentato-reticulospinal projection in the cat

Experiments were performed to anatomically and electrophysiologically demonstrate the existence of a dentato-reticulospinal pathway in the cat. Reticulospinal neurons projecting to the lumbar region of the spinal cord were shown to respond to stimulation in the dentate nucleus at latencies as short as 0.8 ms. The latency of these responses could be varied by changing either stimulus strength or stimulus frequency. Furthermore, intracellular recordings revealed that these responses were associated with a graded depolarization with latencies as short as 0.8 ms. Collision experiments confirmed that the responses recorded in reticular neurons following spinal cord stimulation were antidromically evoked and that the orthodromically evoked responses to dentate stimulation were conducted to the spinal cord. To ensure that the short latency responses evoked in these cells by dentate stimulation were not the result of activating a cerebellar projection to the brainstem through the inferior cerebellar peduncle, an experiment was performed demonstrating that these responses could be blocked by lesions of the brachium conjunctivum. In the anatomical experiments, small injections of horseradish peroxidase limited to the rostromedial region of the medullary reticular formation resulted in the retrograde labeling of neurons in the contralateral dentate nucleus.On the basis of these electrophysiological and neuroanatomical findings, it was concluded that a dentatoreticulospinal system is present in the cat. a system by which the dentate nucleus may affect neuronal integration occurring in the spinal cord.     

Chromogranin A in the olfactory system of the rat

The olfactory bulb of the rat contains chromogranin A at a similar level as the adrenal gland or the hypophysis as revealed by immunoblots. Olfactory chromogranin A also displays the same size as chromogranin A of endocrine cells. In the hippocampus and other brain regions, we could not detect chromogranin A by immunoblotting. In contrast, chromogranin A messenger ribonucleic acid (using S1 nuclease protection assays) was observed in all brain regions examined, including the olfactory bulb. By in situ hybridization histochemistry with a complementary ribonucleic acid probe (280 nucleotides), and by immunocytochemistry, chromogranin A synthesis could be localized to cell bodies of the mitral cell layer, of the external plexiform layer and of the periglomerular region of the olfactory bulb. Immunocytochemically, chromogranin A was also detected in the central projection areas of mitral and tufted cells in the primary olfactory cortex and the anterior amygdaloid area but not in the olfactory glomeruli, where the incoming olfactory nerve fibers of the primary olfactory neurons establish synaptic contacts. Taken together the data show that chromogranin A, following biosynthesis in the perikarya of the mitral and tufted cells, is specifically transported into their axonal terminals but not into their primary dendrites. We propose that the rat olfactory system could serve as a model for the study of chromogranin A regulation and function in neurons.