SAA、CRP联合血常规检测在儿童急性呼吸道感染中的临床应用价值

目的:研究血清淀粉样蛋白A(Serum amyloid A protein,SAA)、C反应蛋白(C reactive protein,CRP)联合血常规检测在儿童急性呼吸道感染中的意义.方法:回顾性分析2019年4月至2020年4月我院收治的424例感染性疾病患儿(细菌感染组133例,病毒感染组167例及支原体感染组124例)及同期本院体检的健康受检儿150例(对照组).比较不同组白细胞(White blood cell,WBC)、CRP及SAA水平及不同检测方法的诊断效能.结果:细菌感染组患儿CRP、WBC以及SAA水平均高于对照组;CRP、WBC水平高于病毒感染以及支原体感染组;SAA水平低于病毒感染、支原体感染组;病毒感染、支原体感染组CRP、SAA水平高于对照组(P<0.05).CRP、WBC、SAA联合检测特异度、敏感度、AUC均高于单一检测法(P<0.05).结论:SAA、CRP及WBC联合检测有利于提高儿童急性上呼吸道感染的诊断准确率.     

血清淀粉样蛋白A1、脂蛋白结合指数预测急性冠脉综合征患者病情严重程度及预后的价值

目的 探讨血清淀粉样蛋白A1(SAA1)、脂蛋白结合指数(LCI)对急性冠脉综合征(ACS)患者病情严重程度及预后的评估价值.方法 选取2016年12月至2019年1月我科收治的130例ACS患者作为研究对象(观察组),同时,另选我院体检健康志愿者80例(对照组).比较各组受试者血清SAA1、LCI水平.根据患者出院后6个月是否发生不良心血管事件(MACE)分为预后良好组和预后不良组.采用COX比例风险模型分析影响ACS患者预后的危险因素,采用受试者工作特征曲线(ROC)分析SAA1、LCI对ACS患者短期预后的评估价值.结果 观察组血清SAA1、LCI水平高于对照组,差异有统计学意义(P＜0.05).ACS重度组血清SAA1、LCI水平高于中度组、轻度组,中度组血清SAA1、LCI水平高于轻度组,差异有统计学意义(P＜0.05).预后不良组患者血清SAA1、LCI水平高于预后良好组患者,差异有统计学意义(P＜0.05).COX多因素回归分析结果显示,Genisis评分、SAA1、LCI是影响ACS患者预后的独立危险因素(P＜0.05).ROC曲线显示,SAA1、LCI预测ACS患者短期预后的曲线下面积分别为0.821、0.840.结论 ACS患者血清SAA1、LCI水平明显升高,与患者病情严重程度及短期预后密切相关,且对ACS患者预后评估有一定预测价值.     

血清SAA、 CRP、 ACE 2对重症胰腺炎的临床诊断价值分析

目的 分析血清淀粉样蛋白A(SAA)、C反应蛋白(CRP)和血管紧张素转化酶2(ACE 2)水平在急性胰腺炎,尤其是重症胰腺炎临床诊断中的应用价值.方法 选取2017年10月至2019年10月本院收治的100例急性胰腺炎患者,其中61例轻症急性胰腺炎为轻症组,39例重症急性胰腺炎为重症组;同时选取同期来院体检的100例健康志愿者为对照组.采集治疗前空腹静脉血,分离血清,采用ELISA实验检测血清SAA、CRP和ACE 2水平.结果 重症组SAA、CRP水平明显高于轻症组,且均高于对照组,差异具有统计学意义(P＜0.05);重症组ACE 2水平明显低于轻症组,且均低于对照组,差异具有统计学意义(P＜0.05).39例重症急性胰腺炎患者血清SAA水平与CRP呈正相关(r=0672,P＜0.01);SAA与ACE 2呈负相关(r=-0.619,P＜0.05);CRP与ACE 2呈负相关(r=-0.525,P＜0.05).三者联合诊断急性胰腺炎和预测重症急性胰腺炎的ROC曲线下面积分别为0.822、0.807.结论 重症急性胰腺炎患者血清SAA、CRP和ACE与病情严重程度具有一定关系,三者联合检测诊断急性胰腺炎和预测重症急性胰腺炎具有较高的临床价值.     

重症高脂血症性急性胰腺炎的危险因素

目的:探讨重症高脂血症性急性胰腺炎(hyperlipidemic acute pancreatitis,HLAP)的危险因素.方法:回顾性分析2017年6月至2020年12月间武汉大学人民医院普外科收治的162例HLAP患者的临床资料,根据严重程度分为轻症与中度重症组(n=89)、重症组(n=73).比较两组一般资料及...     

急性胰腺炎血清CCR、SAA及GGT水平变化在早期病程中的临床意义

目的 探讨肌酐清除率(CCR)、血清淀粉样蛋白A(SAA)及γ-谷氨酰转肽酶(GGT)水平在早期预测急性胰腺炎(AP)疾病程度及病因诊断中的价值.方法 选取2018年6月至2020年6月收治AP患者165例作为AP组,同期非胰胆相关消化道疾病患者60例作为对照组;AP组按照严重程度分为轻度、中度、重度AP组(MAP、S...     

血清淀粉样蛋白A在儿童乙型流感早期诊断中的临床价值探讨

目的 探讨血清淀粉样蛋白A(SAA)、C反应蛋白(CRP)和血常规中白细胞(WBC)计数等指标,在协助儿童乙型流感早期诊断中的临床价值.方法 检测114例乙型流感组患儿和106例对照组儿童的SAA、CRP、血常规中白细胞(WBC)计数水平,计算SAA/CRP比值.分析两组间白细胞(WBC)计数、SAA、CRP、SAA/CRP比值的关系,并采用受试者工作特征曲线(ROC)评价各指标在判断乙型流感病毒感染方面的诊断效能.结果 乙型流感组SAA、SAA/CRP、WBC计数与对照组相比,差异有显著统计学意义(P<0.01).乙型流感组CRP与对照组相比,差异有统计学意义(P=0.01).ROC曲线分析显示,乙型流感组SAA、SAA/CRP的曲线下面积(AUC)均>0.8(P<0.01),灵敏度分别为85.10％、73.70％,特异性分别为80.20％、92.50％.结论 SAA、SAA/CRP比值可作为病毒感染的诊断指标,可以协助早期诊断儿童乙型流感病毒感染,对开展早期治疗具有指导意义,具有一定的临床价值.     

应用Logistic 回归分析炎性因子与子痫前期孕妇病情的相关性

目的:探讨子痫前期(PE)孕妇血清炎性因子IL-17、C反应蛋白(CRP)、血浆淀粉样蛋白A(SAA)的水平变化及与病情的相关性。方法:选取75例子痫前期孕妇作为观察组,其中包括45例轻度子痫患者和30例重度子痫患者,同时选取同期在本院查体的30例健康孕妇作为对照组。用酶联免疫吸附法(ELISA)测定IL-17、SAA的含量,用免疫比浊法测定CRP的含量。应用Logistic回归分析各指标与疾病严重程度的相关性。结果:PE孕妇血清中CRP、IL-17和SAA含量显著高于对照组,重度PE孕妇血清CRP、IL-17和SAA含量显著高于轻度组(P0. 05); Logistic回归分析证实CRP、IL-17和SAA与PE病情具有密切关联(P0. 05)。三者联合检测诊断价值最高。结论:血清CRP、IL-17和SAA参与了PE的发生、发展,可作为判断疾病轻重的指标,为早期检测及治疗提供有利的依据。     

急性脑梗死患者血清淀粉样蛋白A、β2-MG和D-D水平及其临床意义

目的观察急性脑梗死(acute cerebral infarction,ACI)患者淀粉样蛋白A(serum amyloid A,SAA)、β2-微球蛋白(β2-microglobulin,β2-MG)及D-二聚体(D-dimer,D-D)水平变化,并探讨其与ACI的相关性及临床意义。方法选取ACI患者100例及健康体检者30例(对照组),ACI组于入院1d、7d、14d,对照组于体检当天,采集外周静脉血,均行血清SAA、β2-MG、D-D水平的测定。ACI患者均行美国国立卫生研究院卒中量表(National Institute of Health stroke scale,NIHSS)评分,按病灶最大径将患者分别纳入大梗死组(>5cm)、中梗死组(3~5cm)、小梗死组(<3cm),并根据90 d改良Rankin量表(modified rankin scale,mRS)评分,将患者分为预后良好组(≤2分)和预后不良组(>2分),对各组血清SAA、β2-MG、D-D水平进行统计分析。结果ACI组血清SAA、β2-MG、D-D水平随入院时间的延长均呈降低趋势(P<0.05),但入院1d、7d、14d时血清SAA、β2-MG、D-D水平均明显高于对照组(P<0.05)。随着梗死面积增大,ACI患者血清SAA、β2-MG及D-D水平均逐渐增高(P<0.05)。ACI组入院1d、7d、14d时,血清SAA、β2-MG及D-D水平与NIHSS评分均呈正相关(P<0.05)。预后不良组血清SAA、β2-MG及D-D水平均明显高于预后良好组(P<0.05)。ROC曲线分析显示,SAA、β2-MG、D-D水平预测ACI患者预后不良的曲线下面积分别为0.674(95%CI:0.544~0.803)、0.984(95%CI:0.000~1.000)、0.668(95%CI:0.547~0.789)。结论SAA、β2-MG、D-D在ACI患者中表达增高,或许可作为ACI病情严重程度及预后判断的血清标志物。     

C-反应蛋白、血清淀粉样蛋白A和YKL-40在小儿复发性肺炎中的联合检测作用

目的 探讨C-反应蛋白(C-reactive protein,CRP)、血清淀粉样蛋白A(serum amyloid A,SAA)和人软骨糖蛋白39(human cartilage glycoprotein 39,YKL-40)在小儿复发性肺炎中的表达及联合检测作用.方法 选取2016年5月至2017年4月本院收治的小儿复发性肺炎患儿50例为观察一组,本院收治的首次肺炎发作患儿50例为观察二组,和同期体检健康儿50例为对照组.收集受试儿血清并检测血清中CRP、SAA和YKL-40水平.结果 与对照组相比,观察一组和观察二组患儿血清CRP、SAA和YKL-40显著升高;经常规治疗后,观察一组和观察二组患儿血清CRP、SAA和YKL-40水平显著降低,差异具有统计学意义(P＜0.05).患儿血清CRP、SAA和YKL-40联合检出率显著高于各指标单独检出率.结论 CRP、SAA和YKL-40在小儿复发性肺炎患儿血清中呈过表达状态,且CRP、SAA和YKL-40联合检测及实时监测对于诊断重症肺炎具有重要的临床意义.     

冠心病患者血清CRP与SAA的表达及临床意义

目的：检测C-反应蛋白（CRP）与血清淀粉样蛋白（SAA）的水平并分析它们与急性心肌梗死（AMI）及不稳定性心绞痛（UAP）的关系。方法：我们采用酶速率法和ELISA对36例AMI患者、31例UAP患者及41例正常人进行血清CRP与SAA含量检测,并进行统计学分析。结果：AMI及UAP患者血清CRP与SAA水平均明显高于正常对照组（P〈0.01）。结论：CRP与SAA水平与冠心病的严重程度呈正相关,CRP与SAA可作为预测冠心病发生的敏感指标。     

Modes of reinforcement and gender and culture of experimenter and subject: effects of "alien" and external reinforcement for Japanese and American men and women

In this study, we examined external and "alien" reinforcement (ER and AR. respectively) as a factor in social learning, and studied the combined effects of culture and reinforcement mode. A female (Experiment 1) and a male (Experiment 2) experimenters conducted experimental sessions. Both men and women, who grew up in the same culture as the experimenter, participated and performed the experimental task. A three-way interaction effect of experimenter gender, culture, and reinforcement mode was found on task performance. And the effect was more pronounced for a Japanese experimenter. A female and a male experimenters conducted Experiments 3 and 4, respectively; however participants this time were men and women who grew up in different cultures than the experimenter. Results indicated that the pattern of the subject gender and reinforcement mode interaction effect, when the experimenter was Japanese with American subjects, was exactly opposite to that when the experimenter was American. These experiments showed that AR was as effective for social learning as ER, and that the cultural backgrounds of experimenter and subject influenced AR and ER effectiveness.     

Rates of oxygen consumption and of anaerobic glycolysis in renal cortex and medulla of adult and new-born rats and guinea-pigs

1. Rates of oxygen uptake and of anaerobic glycolysis were estimated in slices from the renal cortex and medulla (a) of adult rats and guinea-pigs, (b) of new-born (1-, 5- and 21-day-old) rats and of guinea-pigs of 1, 12, 21, 24 and 120 hr age.2. In the adult rat, Q(O2) values for the cortex were 12.55 +/- 0.20 (22) and for the medulla: 8.56 +/- 0.17 (22) mul./hr.mg dry weight, while in the new-born rat (24 hr old) they were 10.99 +/- 0.46 (12) and 9.33 +/- 0.18 (9) mul./hr.mg dry weight respectively.3. Values for Q(CO2) (N2) (anaerobic glycolysis) in the 14 hr old new-born rat were in the renal cortex 9.65 +/- 0.35 (5) and in the medulla 7.39 +/- 0.43 (5) mul./hr.mg dry weight; while in the adult they were 2.25 +/- 0.08 (16) and 5.76 +/- 0.14 (16) mul./hr.mg dry weight, respectively.4. In the adult guinea-pig values for Q(CO2) (N2) were of the same order as in the adult rat, though the rate of O(2) uptake was for the cortex 8.12 +/- 0.22 (12) and for the medulla 5.02 +/- 0.23 (11) mul./hr.mg dry weight.5. Though the Q(O2) values in the renal cortex and medulla were smaller in the 1 hr old new-born guinea-pig, they were already increasing in the 12 hr old neonate.6. The results are discussed in the light of enzyme changes occurring during the process of maturation of the nephron as indicated by histochemical observations.     

Timing and intensity of early fevers and the development of allergies and asthma

BACKGROUND: Early childhood fevers appear to protect against later allergies and asthma. What is not known is the time in which fevers exert this effect and whether the degree of temperature increase is important. OBJECTIVE: We sought to examine the relationship between the time and degree of early fevers and later allergies and asthma. METHODS: Eight hundred thirty-five children from southeast Michigan were enrolled at birth. Clinic records from their first 2 years were abstracted for episodes of fever. At age 6 to 7 years, children underwent allergy testing. We examined fevers occurring within 6-month intervals in the first 2 years of life and outcomes at age 6 to 7 years. The primary outcome measures were allergic sensitization, asthma, asthma with allergic sensitization, and asthma without allergic sensitization. RESULTS: In the unadjusted analysis each episode of fever between 7 and 12 months of age was associated with a lower odds of allergic sensitization (odds ratio [OR], 0.71; 95% CI, 0.54-0.93) and asthma with allergic sensitization (OR, 0.43; 95% CI, 0.21-0.90) at age 6 to 7 years. Likewise, every 1 degrees C increase in the maximum temperature between 7 and 12 months was associated with a lower odds of allergic sensitization (OR, 0.77; 95% CI, 0.61-0.96) and asthma with allergic sensitization (OR, 0.62; 95% CI, 0.40-0.94). After adjusting for potential confounders, each episode of fever between 7 and 12 months was associated with a lower likelihood of asthma with allergic sensitization (adjusted OR, 0.33; 95% CI, 0.11-0.94) at age 6 to 7 years. CONCLUSIONS: Both the timing and intensity of childhood fevers appear to be important factors in the development of allergies and asthma.     

纳米水平分子成像与诊疗一体化研究进展与挑战

分子成像能以非侵入性的方式重现活体细胞的生理功能和生物学过程,提高疾病的早期和特异性诊断水平。纳米颗粒/材料具有物理性质可控性高、易于表面修饰、血液循环时间长和可功能化等优点,在疾病诊断与治疗中显示出巨大潜力。但如何阐明纳米材料多功能间的内在联系、解决其代谢及安全性等关键机制难题、实现纳米颗粒/材料多功能性到临床多功能...