大鼠皮瓣血液循环的磁共振波谱学初步研究

观察皮瓣的能量代谢。方法：利用磁共振波谱技术分别对大鼠皮瓣在正常灌注、动脉阻流、静脉阻流、失活等条件下ＡＴＰ、ＰＣｒ、Ｐｉ峰形的变化进行分析。结果正常时,Ｐｉ低于ＡＴＰ的γ及α峰。     

γ-sm/Act-PSA与PSA联合判定法在前列腺疾病中的临床应用意义

目的 探讨鉴别前列腺癌（ＰＣＡ）和前列腺特异抗原（ＰＳＡ）值增高的良性前列腺增生症（ＢＰＨ）判定方法,提高ＰＣＡ的诊断率及前列腺活检的阳性率。方法：ｈｙｂｒｉｔｅｃｈ法测定１９例ＰＣＡ和１９例ＢＰＨ血清中结合ＰＳＡ（Ａｃｔ－ＰＳＡ）、γ－ｓｍ（γ－ｓｅｍｉｏｎｏｐｒｏｔｅｉｎ）水平,初步探讨γ－ｓ／Ａｃｔ－ＰＳＡ界值。结果 γ－ｓｍ／Ａｃｔ－ＰＳＡ平均值：ＰＣＡ组０．７０５,ＢＰＨ组１．４０５,差     

正常人肝细胞中HBV-PHSA受体基因表达的研究

作者根据乙型肝炎病毒（ＨＢＶ）ａｄｒ型ＰｒｅＳ２基因序列设计合成一对聚合酶链反应（ＰＣＲ）引物，经特异性试验后，用于扩增正常人肝细胞中多聚人白蛋白受体（ＰＨＳＡｒ）基因转录产物。结果：ＲＴ－ＰＣＲ及ＲＮＡ－ＰＣＲ扩增结果均为阳性，而相应骨骼肌总ＲＮＡ扩增结果呈阴性，提示乙型肝炎病毒ＰｒｅＳ２区与ＰＨＳＡｒ基因外显子序列具有一定程度的同源性；同时表明ＰＨＳＡｒ基因在肝组织中处于表达状态，而对ＨＢＶ不敏感的组织如骨骼肌则不表达。该研究结果与其它ＨＢＶ－ＰＨＳＡｒ研究相比，进一步证实了ＰＨＳＡｒ在ＨＢＶ感染肝细胞的作用。     

羟乙膦酸钠对实验性高钙尿症的防治作用及其机制

目的：观察骨吸收抑制剂羟乙膦酸钠对实验性高钙尿症的防治作用,并其可能机制。方法：建立大鼠实验性高钙尿症模型,观察羟乙膦酸钠治疗后２周和４周的尿钙,Ｃａ＾２＋／Ｃｒ,ＮＡＧ／Ｃｒ,以及肾和十二揸肠Ｃａ＾２＋－ＡＴＰ酶（钙泵）的变化。结果：２周时尿钙,Ｃａ＾２＋／Ｃｒ,血ＡＬＰ量有所升高,但４周时尿钙,Ｃａ＾２Ｃｒ,ＮＡＧ／Ｃｒ及血ＡＬＰ均显著下降（Ｐ〈０．０１）,２４小时尿一量与尿ＮＡＧ／Ｃｒ值呈正     

正常人肝细胞中HBV—PHSA受体基因表达的研究

作者根据乙型肝炎病毒（ＨＢＶ）ａｄｒ型ＰｒｄＳ２基因序列设计合成一对聚合酶链反应（ＰＣＲ）引物，经特异性试验后，用于扩增正常人肝细胞中多聚人白蛋白受体（ＰＨＳＡｒ）基因转录产物。结果：ＲＴ－ＰＣＲ及ＲＮＡ－ＰＣＲ扩增结果均为阳性，而相应骨骼肌总ＲＮＡ扩增结果呈阴性，提示乙型肝炎病毒ＰｒｅＳ２区与ＰＨＳＡｒ基因外显子序列具有一定程度的同源性；同时表明ＰＨＳＡｒ基因在肝组织中处于表达状态，而对ＨＢＶ不     

自发性高血压大鼠血管平滑肌细胞缺氧预处理的延迟保护作用

目的；观察缺氧预处理（ＰＣ）对于自发性高血压大鼠（ＳＨＲ）血管平滑肌细胞（ＶＳＭＣ）缺氧复氧（Ｈ／Ｒ）损伤的延迟保护作用，并探讨其可能机制，方法：对ＶＳＭＣ于缺氧ＰＣ２４ｈ后进行Ｈ（２ｈ）／Ｒ（１ｈ）结束实验时测定其Ｈ／Ｒ后细胞存活率，乳酸脱氢酶（ＬＤＨ）释放和ＡＴＰ含量，并测定其金属硫蛋白（ＭＴ）量，结果：缺氧ＰＣ明显减轻Ｈ／Ｒ造成的ＳＨＲ的ＶＳＭＣ存活率下降，ＡＴＰ耗竭和ＬＤＨ释放，并使细胞内     

肢体缺血再灌注损伤局部与主要器官脂质过氧化的观察

目的：观察兔一侧肢体缺血再灌注损伤后局部与主要器官脂质过氧化改变。方法：利用新西兰兔一侧后肢造成缺血再灌注损伤模型。实验分假手术组（Ｓｈａｍ）。缺血组（ＩＳＣ）和缺血－再灌注组（Ｉ／Ｒ）。分别取胫骨前肌标本测定三磷酸腺苷（ＡＴＰ），磷酸肌酸（ＰＣｒ）及丙二醛（Ｍｏｒｔ）含量，测定超氧化物岐化酶（ＳＯＤ）和肌浆网膜钙泵（Ｃａ２＋－ＡＴＰａｓｅ）活力，取心、肺、肾组织测ＭＤＡ及ＳＯＤ。结果：Ｉ／Ｒ组各项指标改变明显，与Ｓｈａｍ组及ＩＳＣ组相比，骨骼肌ＡＴＰ、ＰＣｒ明显下降，ＭＤＡ水平明显升高，ＳＯＤ，Ｃａ２＋－ＡＴＰａｓｅ活力明显减低，心、肺、肾ＭＤＡ水平明显升高，ＳＯＤ活力明显下降。结论：一侧肢体缺血再灌注后，可导致肌肉组织能量代谢障碍及出现脂质过氧化损害，同时主要器官亦出现类似改变。这种损伤可能是局部严重创伤后造成多器官衰竭的一条重要途径。     

核磁共振^31P谱研究川芎嗪对细胞高能磷酸盐代谢的影响

利用核磁共振￣（３１）Ｐ普测定鼠肌细胞内α－ＡＴＰ、β－ＡＴＰ、γ－ＡＴＰ、肌酸磷酸盐以及无机磷酸盐的相对含量随时间的变化时，发现注射川芎嗪的小鼠，其肌细胞前四种高能磷酸盐的代谢速率明显高于对照组。此法具有快速和非破坏性的优点，可进行细胞内高能磷酸盐代谢的动态观察。     

细胞因子抗体及地塞米松对小鼠脓毒血症心肌及骨骼 …

目的 观察和比较细胞因子抗体及地塞米松（ＤＸＭ）对脓毒血症心肌及骨骼骨细胞腺苷三磷酸（ＡＴＰ）含量的影响。方法 制作小收肠结扎穿孔（ＣＬＰ）脓毒血症模型，分别注射生理盐水、ＩＬ－１βＡｂ、ＴＮＦαＡｂ、ＩＬ－１γＡｂ、ＩＬ－１βＡｂ＋ＴＮＦαＡｂ＋ＩＦＮγＡｂ，以及ＤＸＭ，观察各组小鼠心肌及骨骼肌ＡＴＰ含量。结果 ①ＣＬＰ模型心肌及骨骼肌细胞ＡＴＰ含量严重减少。②ＩＬ－１βＡｂ、ＴＮＦαＡｂ使ＣＬ     

自发性高血压大鼠血管平滑肌细胞缺氧预处理的延迟保护作用

目的：观察缺氧预处理（ＰＣ）对于自发性高血压大鼠（ＳＨＲ）血管平滑肌细胞（ＶＳＭＣ）缺氧复氧（Ｈ／Ｒ）损伤的延迟保护作用，并探讨其可能机制。方法：对ＶＳＭＣ于缺氧ＰＣ２４ｈ后进行Ｈ（２ｈ）／Ｒ（１ｈ），结束实验时测定其Ｈ／Ｒ后细胞存活率、乳酸脱氢酶（ＬＤＨ）释放和ＡＴＰ含量，并测定其金属硫蛋白（ＭＴ）量。结果：缺氧ＰＣ明显减轻Ｈ／Ｒ造成的ＳＨＲ的ＶＳＭＣ存活率下降、ＡＴＰ耗竭和ＬＤＨ释放，并使细胞内应激蛋白ＭＴ含量升高１．３倍，上述指标与正常血压对照组ＷＫＹ大鼠ＶＳＭＣ的结果相近。结论：培养的ＳＨＲ的ＶＳＭＣ亦存在ＰＣ的延迟保护作用，其保护机制涉及ＭＴ合成增多。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.