术前血清乳酸脱氢酶与白蛋白比值对脑胶质瘤患者预后影响的研究

目的 探讨术前血清乳酸脱氢酶(lactate dehydrogenase, LDH)与白蛋白(albumin, ALB)比值(lactate dehydrogenase to albumin ratio, LAR)对脑胶质瘤患者预后的影响。方法 回顾性分析101例胶质瘤患者的临床资料;采用受试者工作特征(receiver operating characteristics, ROC)曲线分析LAR最佳临界值,分为高LAR组与低LAR组;采用Kaplan-Meier法绘制生存曲线;纳入性别、年龄、KPS评分、肿瘤直径、肿瘤病理分级、肿瘤切除程度、术后放疗、术后化疗和LAR因素进行统计学分析。采用COX单因素及多因素分析研究LAR与胶质瘤无进展生存期(progress free survival, PFS)和总生存期(overall survival, OS)之间的相关性,评估术前LAR对脑胶质瘤患者预后的临床价值。结果 高LAR组的PFS和OS均短于低LAR组(P<0.05)。COX单因素分析显示,肿瘤病理级别、切除程度、放疗、化疗、术前LAR水平是影响胶质瘤患者PFS和OS的危险...     

乳酸脱氢酶白蛋白比值对重症肺炎患者并发弥散性血管内凝血的预测价值

目的 探讨乳酸脱氢酶（lactate dehydrogenase,LDH）与白蛋白（albumin,Alb）比值（lactate dehydrogenase to albumin ratio,LAR）对重症肺炎并发DIC的预测价值。方法 采用回顾性研究方法,选择2018年1月—2023年3月常州市第二人民医院重症监护病房（intensive care unit,ICU）收治的312例重症肺炎患者,收集所有患者的性别、年龄、基础疾病等临床资料及采集患者入院首次LDH、Alb等指标,同时计算LAR、24 h内序贯器官衰竭评分和急性生理学和慢性健康状况评价Ⅱ。研究主要结局是重症肺炎有无并发弥散性血管内凝血（disseminated intravascular coagulation,DIC）,次要结局是重症肺炎并发DIC后30 d内死亡情况。采用单因素和多因素Logistic回归分析影响重症肺炎患者发生DIC的危险因素,并绘制受试者操作特征曲线（receiver operator characteristic curve,ROC曲线）,计算ROC曲线下面积（area under ROC cu...     

纤维蛋白原/白蛋白对活动期和预后不良系统性红斑狼疮的预测价值

目的 研究血浆纤维蛋白原/白蛋白(fibrinogen to albumin ratio, FAR)对活动性系统性红斑狼疮(systemic lupus erythematosus, SLE)、重度活动性SLE以及预后不良SLE的预测价值。方法 回顾性分析作者医院治疗的SLE患者143例,根据SLE疾病活动指数(SLE disease activity index, SLEDAI)将患者分为稳定期、活动期,重度活动性SLE、非重度活动性SLE,临床缓解、预后不良组。通过受试者工作特征(receiver operating characteristic, ROC)曲线分析FAR对活动性SLE、重度活动SLE、预后不良的预测价值。结果 活动期SLE患者SLEDAI、乳酸脱氢酶(lactate dehydrogenase, LDH)、甘油三酯(triglyceride, TG)、纤维蛋白原(fibrinogen, FIB)、FAR、血沉(erythrocyte sedimentation rate, ESR)、抗dsDNA和抗dsDNA阳性率均高于稳定期,白蛋白(albumin, ALB)、...     

肿瘤中乳酸脱氢酶B作用机制的研究进展

有氧糖酵解增加是肿瘤代谢重编程的主要特征。有氧糖酵解不仅为癌细胞提供能量而且为其生物合成提供必要的前体,这对促进肿瘤生长非常重要。癌细胞通过对糖酵解酶进行调节来满足其自身需求,糖酵解酶在促进肿瘤存活、转移、侵袭等方面发挥着积极作用。乳酸脱氢酶（lactate dehydrogenase,LDH）作为有氧糖酵解的关键酶,由乳酸脱氢酶A(lactate dehydrogenase A,LDHA)和乳酸脱氢酶B(lactate dehydrogenase B,LDHB)两种亚基组成。LDHA被认为在有氧糖酵解过程中起着关键作用,并得到了广泛的研究,然而对于LDHB的研究却较少。但目前LDHB在各种肿瘤进展中的重要作用已被越来越多的报道,大量研究表明,LDHB在多种肿瘤中异常表达,与肿瘤恶性进展相关。本文从LDHB在肿瘤中的调控机制、与肿瘤发生发展的关系以及作为肿瘤诊断的生物标志物等方面综述了其近10年的研究进展,有助于深入了解该蛋白在肿瘤中的作用机制。     

Central Venous Catheter-related Thrombosis in Senile Male Patients: New Risk Factors and Predictors

Central venous catheterization(CVC)-related venous thrombosis is a common but serious clinical complication, thus prevention and treatment on this problem should be extensively investigated. In this research, we aimed to investigate the incidence rate of CVC-related venous thrombosis in senile patients and give a further discussion on the related risk factors and predictors. A total of 324 hospitalized senile male patients subjected to CVC were selected. Retrospective investigation and analysis were conducted on age, underlying diseases, clinical medications, catheterization position and side, catheter retention time, and incidence of CVC-related venous thrombosis complications. Basic laboratory test results during catheterization and thrombogenesis were also collected and analyzed. Among the 324 patients, 20 cases(6.17%) of CVC-related venous thrombosis were diagnoseds. The incidence rate of CVC-related venous thrombosis in subclavian vein catheterization was significantly lower than that in femoral vein catheterization(P<0.01) and that in internal jugular vein catheterization(P<0.05). No statistically significant difference was found between femoral vein catheterization and internal jugular vein catheterization(P>0.05). Previous venous thrombosis history(P<0.01), high lactate dehydrogenase level(P<0.01), low high-density lipoprotein(HDL) level(P<0.05), and low albumin level(P<0.05) were found as risk factors or predictors of CVC-related venous thrombosis in senile male patients. Subclavian vein catheterization was the most appropriate choice among senile patients to decrease the incidence of CVC-related venous thrombosis. Previous venous thrombosis history, high lactate dehydrogenase level, low HDL level, and low albumin level were important risk factors in predicting CVC-related venous thrombosis.     

当归红芪超滤物对急性心肌梗死大鼠的保护作用及对热休克蛋白70表达的影响

目的 探讨当归红芪超滤物对急性心肌梗死大鼠乳酸脱氢酶(actate dehydrogenase,LDH)、乳酸脱氢酶同工酶(lactate dehydrogenase,LDH1)和热休克蛋白70 (heat shock protein 70,HSP70 mRNA) 表达的影响,阐明当归红芪超滤物对梗死心肌的保护作用.方...     

A Review on Molecular Physiology of Malate and Lactate Dehydrogenases in Fishes

The malate(EC 1.1.1.37)and lactate(EC 1.1.1.27)dehydrogenases are themetabolic enzymes directly or indirectly involved in energy production,gluconeogenesis and lipogenesis.Malate dehydrogenase(MDH)exists in twoisoenzymic forms,cytoplasmic(cMDH)and mitochondrial(mMDH),composed of Aand/or B subunits(dimeric molecule:MW 40,000-120,000).Lactate dehydrogenase(LDH)has tetrameric(MW 35,000-110,000)structure made up of either A and/or B,orC(C,E,F)subunits.They catalyze an ordered bisubstrate(substrate and coenzyme)     

血清VitB12、叶酸及LDH检测在骨髓增生异常综合征和巨幼红细胞性贫血鉴别诊断中的价值

目的 探讨血清维生素B12(Vitamins B12,VitB12)、叶酸及乳酸脱氢酶(lactate dehydrogenase,LDH)检测在骨髓增生异常综合征(myelodysplastic syndrome,MDS)及巨幼红细胞性贫血(megaloblastic anemia,MA)鉴别诊断中的价值.方法 回顾...     

乳酸脱氢酶同工酶琼脂糖电泳法的某些改进

目前国内有多种测定乳酸脱氢酶(lactate dehydrogenase, LD, LDH)同工酶的方法,但由于显色后的区带不甚清晰,故结果不能令人满意。用琼脂糖凝胶电泳法可克服上述缺点,但操作较繁并需较长时间。我们通过实践作某些改进,现介绍如下: 材料与方法 一、材料 (一)标本:健康人血清。 (二)仪器:1.电泳仪(DY-1型电泳仪);2.721型分光光度计。     

Effect of Jianpi Huoxue decoction (健脾活血方)-containing serum on tumor necrosis factor-α secretion and gene Expression of endotoxin receptors in RAW264.7 cells induced by lipopolysaccharide

Objective:To evaluate the effect of Jianpi Huoxue decoction(健脾活血方,JHD)-containing serum on tumor necrosis factor-α(TNF-α) secretion and endotoxin receptor gene expression in RAW264.7 cells induced by lipopolysaccharide(LPS).Methods:The cytotoxicity of blank-control serum and JHD-containing serum at different concentrations were evaluated through the lactate dehydrogenase(LDH) assay in RAW264.7 cells.RAW264.7 cells were divided into six groups:5%blank-control serum group(C1,n=3),5%blank-control serum plus...     

相关临床指标在初诊多发性骨髓瘤中的预测价值

目的：评估初诊多发性骨髓瘤(multiple myeloma, MM)患者中性粒细胞与淋巴细胞比值(neutrophil/lymphocyte ratio, NLR)、血清游离轻链比值(serum free light chain ratio, r FLC)、β2微球蛋白(β2-microglobulin, β2-MG)、乳酸脱氢酶(lactate dehydrogenase, LDH)、铁蛋白(serum ferritin, SF)、白蛋白(albumin, ALB)、血红蛋白(hemoglobin, Hb)、血尿酸(uric acid, UA)、血钙(serum calcium, Ca)等临床指标对不同临床分期分组及不同转归的预测价值。方法：收集南通大学第二附属医院2018年1月—2020年1月有rFLC检测记录的初诊MM患者58例的临床资料,对患者进行国际分期系统(International Staging System, ISS)分期,根据有无肾功能损害分为A组与B组;有无髓外病变分为无髓外病变组与髓外病变组;4次诱导治疗后患者分为完全缓解(complete response,...     

51例重症新型冠状病毒肺炎临床特征及预后分析

目的:探讨重症新型冠状病毒肺炎(coronavirus disease 2019,COVID-19)确诊病例临床特征、治疗及预后。方法:选取2020年1月21日至2月25日重庆三峡中心医院收治的确诊重症COVID-19患者51例。回顾性分析患者一般资料、临床表现、实验室指标、影像学资料、并发症、治疗及预后情况。结果:51例COVID-19患者中,重型35例(68.6%),危重型16例(31.4%)。51例患者首发症状主要以发热、咳嗽、咳痰、呼吸困难、乏力为主。危重型组呼吸频率快于重型组(P=0.027);危重型组中性粒细胞比率高于重型组(P=0.012);危重型组乳酸脱氢酶(lactate dehydrogenase,LDH)高于重型组(P=0.028);危重型组D-二聚体高于重型组(P=0.013);危重型组降钙素原(procalcitonin,PCT)高于重型组(P=0.013);危重型组白蛋白低于重型组(P=0.004);危重型组氧合指数(partial pressure of oxygen/fraction of inspired oxygen,PaO₂/...     

复制型HBV-Tg小鼠原代肝细胞的培养

目的 建立复制型HBV-Tg原代肝细胞体外培养方法,观察HBV-Tg小鼠原代肝细胞的氧化应激敏感性.方法 改良的两步灌注法分离、纯化、培养HBV-Tg小鼠原代肝细胞,24孔板培养7天,每天换液,收集上清,检测乳酸脱氢酶(lactate dehydrogenase,LDH)、丙氨酸转移酶(alanine transami...     

小词典

ADH alcohol dehydrogenase 醇(乙醇)脱氢酶Asp aspartic acid 天冬氨酸BSA bovine serum albumin 牛血清白蛋白BSLT the brine shrimp lethality test 鳃足虫致死实验DHT dehydrotestostrone 双氢睾酮DMBA 7,12-dimethylbenz(a)anthracene 7,12-二甲基苯并蒽GA gibberellic acid 赤霉酸MEPP miniature end plate potential 微终板电位     

癫痫后横纹肌溶解致急性肾功能衰竭1例

横纹肌溶解症(rhabdomyolysis,RM)是指横纹肌损伤后大量肌细胞内容物进入外周血,如肌红蛋白(myoglobin,Mb)、肌酸激酶(creatine,CK)、乳酸脱氢酶(lactate dehydrogenase,LDH)等酶类及离子和小分子毒性物质,引起组织器官损伤的一组临床综合征。横纹肌溶解产生的内容物可直接损伤肾小管和阻塞肾小管,据报道,RM患者中有10%~50%可引     

哈夫病肾损伤肾活检病例1例报道

<正>哈夫病是由于食用甲壳类或鱼类,导致骨骼肌细胞受损而发生的横纹肌溶解综合征[1-2]。发病后,患者血清中肌酸激酶(creatine kinase,CK)、肌酸激酶同工酶(creatine kinase isoenzyme,CK-MB)、乳酸脱氢酶(lactate dehydrogenase,LDH)、肌红蛋白(myoglobin,MYO)等迅速升高,尤其是MYO在经过肾脏排出体外过程中,可能造成肾小管堵塞,引起肾损     

基于MIMIC数据库分析乳酸脱氢酶/白蛋白比值与脓毒性休克合并急性肾损伤患者预后的相关性

目的 探讨血清乳酸脱氢酶/白蛋白比值(lactate dehydrogenase to albumin ratio,LAR)与脓毒性休克合并急性肾损伤(acute kidney injury,AKI)患者预后的相关性。方法 回顾性分析美国重症监护数据库中在重症医学科(intensive care unit,ICU)住院的脓毒性休克伴AKI患者,根据30天预后,将患者分为生存组及死亡组,比较两组临床基线资料的差异;并根据限制性立方样条(Restricted cubic spline,RCS)分析时危险比(hazard ratio,HR)为1时的LAR值(13.53)将患者分为低LAR组和高LAR组,比较两组30天累计生存率的差异;通过RCS分析来比较两组30天死亡风险的差异,进一步采用 Cox比例风险回归模型分析LAR与患者30天预后的相关性。结果 本研究最终纳入1289例患者。通过比较发现,死亡组LAR较生存组更高(P＜0.05);RCS分析显示,LAR与脓毒性休克合并AKI患者30天全因死亡风险整体呈非线性趋势关系(c2= 62.530,P<0.001)。随着LAR的升高,患者30天住院期间全因死亡风险随之升高。Kaplan?Meier生存曲线提示,与低LAR组比较,高LAR组30天累计生存率更低(log-rank检验,c2= 58.880, P<0.001);多因素Cox回归分析得出高LAR(≥13.53)是脓毒性休克合并AKI患者30天全因死亡率增高的独立危险因素(P＜0.001)。结论 入ICU时高LAR(≥13.53)是脓毒性休克合并AKI患者30天全因死亡率增高的独立危险因素,但结论仍需更大样本量前瞻性研究来证实。     

小细胞肺癌长期生存患者的临床病理特征及预后分析

目的:分析小细胞肺癌长期生存患者的临床、病理特征,探讨长期生存的预后影响因素。方法:收集2013年1月至2017年1月在福建医科大学附属泉州第一医院确诊并治疗的124例小细胞肺癌患者,按总生存时间(overall survival,OS)分为长期生存组(>24个月)和短期生存组,分析比较2组的临床病理特征,并对长期生存的影响因素进行Kaplan-Meier法生存分析和Cox比例风险模型多因素分析。结果:124例小细胞肺癌患者,24例(19.4%)OS>24个月。长期生存组的局限期、无肝转移患者比例高于短期生存组(P<0.05),而性别、年龄、身高、体质量、脑转移、其他部位转移、血清乳酸脱氢酶(lactate dehydrogenase,LDH)2组间差异无统计学意义(P>0.05);长期生存组的TTF-1阳性、Ki67低表达比例患者高于短期生存组(P<0.05);长期生存组患者对初始化疗更为敏感(P<0.05)。生存分析显示,局限期、无肝转移、TTF-1阳性表达、Ki67低表达、对初始化疗敏感的患者中位OS较高(P<0.05);多因素分析显示,...     

雌性大鼠不同组织中乳酸脱氢酶同工酶组分分布特性

乳酸脱氢酶(lactate dehydrogenase,LDH)的同工酶,是由两种不同的多肽链组成的四聚体。操纵这两种多肽(A 和 B)合成的基因,分别定位在11P_(12)和12P_(12)上。多肽一旦合成,在不同组织中能相互组成5种聚合体同工酶,即 LDH_1(B_4)、LDH_2(B_3A)、LDH_3(B_2A_2)LDH_4(BA_3)、LDH_5(A_4),其组合形式完全由遗传基因决定和调控。同工酶在器官、组     

Phloretin-induced suppression of oxidative and nitrosative stress attenuates doxorubicin-induced cardiotoxicity in rats

Objective:To compare the cardioprotective efficacy of equimolar doses(50 mM/kg,p.o.)of phloretin and genistein against doxorubicin-induced cardiotoxicity in rats.Methods:Cardiotoxicity was induced in rats by intraperitoneal injection of 6 mg/kg doxorubicin on alternative days till the cumulative dose reached 30 mg/kg.This study included four treatment groups of rats(n=6):the control group(0.5%carboxymethyl cellulose solution-treated),the doxorubicin-treated group(0.5%carboxymethyl cellulose solution along with doxorubicin),the genistein-treated group(50 mM/kg/day;p.o.along with doxorubicin)and phloretin-treated group(50 mM/kg/day;p.o.along with doxorubicin).On the 10th day of dosing,rats were anesthetized for recording ECG,mean arterial pressure,and left ventricular function.Oxidative stress,nitric oxide levels,and inflammatory cytokines were estimated in the cardiac tissue.Cardiac function parameters(creatine kinase MB,lactate dehydrogenase,aspartate aminotransferase,and alanine transaminase)were estimated in the serum samples.Results:Phloretin treatment inhibited doxorubicin-induced oxidative stress and also reduced nitric oxide levels in cardiac tissues of rats.Phloretin administration attenuated doxorubicin-induced alterations in hemodynamic parameters(heart rate,mean arterial blood pressure,and left ventricular function)and suppressed the expression of pro-inflammatory cytokines.The cardiac injury markers like creatine kinase MB,lactate dehydrogenase,aspartate aminotransferase,and alanine transaminase were reduced by both genistein and phloretin.All these effects of phloretin were more prominent than genistein.Conclusions:Phloretin offers cardioprotection that is comparable to genistein,a clinically validated cardioprotectant against doxorubicin-induced cardiotoxicity.Further studies are needed to confirm and establish the therapeutic utility of phloretin as a chemopreventive adjuvant to doxorubicin chemotherapy.