慢性锑中毒患者细胞因子水平与抗氧化能力相关分析

目的探讨慢性锑中毒患者细胞因子水平与抗氧化能力的相关性。方法对慢性锑中毒患者和对照组血清白细胞介素-2(IL-2)、白细胞介素-8(IL-8)、肿瘤坏死因子α(TNF-α)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)水平进行检测及分析。结果与对照组相比,慢性锑中毒患者血清IL-2I、L-8和TNF-α水平显著增高(P<0.01),SOD、GSH-Px活性明显降低,而MDA水平增高(P<0.01)。锑中毒患者IL-2I、L-8、TNF-α含量与SOD、GSH-Px活性呈负相关(P<0.01),与MDA水平呈正相关(P<0.01)。结论慢性锑中毒患者的细胞因子水平随抗氧化能力的降低而升高。     

依达拉奉联合还原型谷胱甘肽对急性脑梗死的干预及影响

目的：探讨依达拉奉联合还原型谷胱甘肽对急性脑梗死患者氧化应激指标及炎症介质水平的影响,并作临床预后评估。方法：选择急性脑梗死患者120例,随机分为联合组和对照组各60例,分别测定人院时、治疗后血清丙二醛（MDA）、超氧化物歧化酶（s0D）、白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的水平,并进行临床神经功能缺损程度评分;并以60例健康体检者作为正常组。结果：急性脑梗死患者血清MDA、IL-6和TNF-α水平较正常组升高（P均〈o．05）而SOD水平较正常组降低（P〈．05）;治疗后联合组较对照组血清MDA、IL-6和TNF-α水平及神经功能缺损程度评分下降更为明显（P均〈O．05）而SOD水平较正常组上升明显（P〈O．05）。结论：MDA、SOD、IL-6和TNF-α可能参与了脑梗死的一系列病理、生理变化;联合应用依达拉奉和还原型谷胱甘肽可明显降低血清氧化应激指标及炎症介质水平,并有助于脑梗死后神经功能的恢复。     

慢性非细菌性前列腺炎患者血清细胞因子水平与病原体感染的关系

目的 探讨血清细胞因子与引起慢性非细菌性前列腺炎（CAP）病原体的关系。方法 用ELISA法对112例CAP患者（其中CAPⅠ组80例，CAPⅡ组32例）血清进行IL-2、IL-4、IL-8、TNF-α检测，PCR法检查衣原体、支原体、病毒（HPV、HSV），同时，以20例健康男性作正常对照，并对所取得的数据进行统计学分析。结果 （1）CAPⅠ组血清中的IL-2、IL-4、IL-8、TNF-α均有不同程度的升高，与CAPⅡ组和正常对照组相比差异显著（P〈0．01或P〈0．05），CAPⅡ组血清中IL-2、IL-4、TNF-α与正常对照组比较无显著性差异（P〉0．05）。（2）衣原体、支原体感染患者血清中的IL-2、IL-4、TNF-α与对照组相比差异显著（P〈0.01或P〈0.05），病毒感染患者血清中的IL-2、TNF-α与对照组比较有显著性差异（P〈0．01或P〈0．05），未检出病原体患者血清中的IL-2、IL-4、IL-8、TNF-α与对照组比较差异无显著性（P〉0．05）。结论 血清IL-2、IL-4、IL-8、TNF-α水平与CAP病原体间有-定的相关性，它们在CAP发生发展过程中可能起着重要作用，对CAP辅助性诊断有较大应用价值。     

慢性肾炎患者治疗前后血清IL-2、IL-6和TNF-α的检测及意义

目的探讨慢性肾炎患者治疗前后血清白细胞介素-2（IL-2）、白细胞介素-6（IL6）和肿瘤坏死因子-α（TNF-α）的水平变化及意义。方法选择我院2007年3月-2011年3月期间收治的慢性肾炎患者40例及同期健康体检者50例（对照组）,采用放射免疫法测定两组血清IL-2、IL-6和TNF-α的水平。结果慢性肾炎组治疗前的血清IL-6和TNF-α水平均显著高于对照组（P〈0.05）,而IL-2则显著低于对照组（P〈0．05）;经治疗后,上述指标与对照组相比较,差异仍具有统计学意义（P〈0．05）。结论检测慢性肾炎患者血清IL-2、IL-6和TNF-α水平的变化,对观察患者病情及预后判断等均具有十分重要的临床意义。     

乌司他丁对五步蛇咬伤患者机体氧化应激及炎症反应的影响

目的：探讨乌司他丁对五步蛇咬伤中毒后氧化应激及炎症反应的影响。方法：98例五步蛇咬伤患者随机分成对照组（n＝48）和观察组（n＝50）;对照组给予抗蛇毒血清及支持对症治疗,观察组在对照组治疗基础上加用乌司他丁,治疗5d,分别于患者入院24小时内、给药后1d、给药后3d及给药后5d检测血清超氧化物歧化酶（SOD）、丙二醛（MDA）、肿瘤坏死因子-α（TNF-α）及白细胞介素6（IL-6）的水平。结果：与治疗前相比,患者在治疗后各时间点TNF-α、IL-6及MDA水平均显著降低（P＜0．05）,但观察组下降更明显（P＜0．01）;SOD水平均升高（P＜0．05）,但观察组升高更明显（P＜0．01）;与对照组相比,治疗后观察组各时间点TNF-α、IL-6及MDA水平均降低（P＜0．05）,SOD水平均升高（P＜0．05）。结论：乌司他丁可降低五步蛇咬伤患者血清TNF-α、IL-6及MDA水平,升高SOD水平,减轻氧化应激及抑制炎症反应,因此有利于减轻五步蛇咬伤所致中毒的损害。     

百草枯中毒患者血清细胞因子与抗氧化能力相关性分析

目的探讨百草枯中毒(Paraquat posisoning,PQP)患者血清细胞因子与抗氧化能力相关性。方法检测PQ患者血清白细胞介素-2(IL-2)、白细胞介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)水平,及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GsH-Px)、丙二醛(MDA)含量,并进一步进行比较分析。结果 PQP患者血清IL-2、IL-8及TNF-α明显高于正常人群,差异具有统计学意义(P<0.05);PQP患者GSH-px和SOD活性明显降低,而血清MDA水平明显增高,差异具有统计学意义(P<0.05);PQP患者IL-2、IL-8和TNF-α含量与SOD、GSH-px之间存在负相关(P<0.05),与MDA间存在正相关(P<0.05)。结论 PQP患者细胞因子水平随机体抗氧化能力的降低而升高。     

急性一氧化碳中毒患者血清TNF—α、IL-6、IL-8、IL-10水平变化及高压氧治疗的影响

目的探讨急性一氧化碳中毒（ACOP）患者血清肿瘤坏死因子-α（TNF—α）、白细胞介素-6（IL-6）、白细胞介素-8（IL-8）、白细胞介素-10（IL-10）水平变化及高压氧（HBO）治疗对其的影响。方法72例中、重度ACOP患者分为常压氧（NBO）治疗组32例及高压氧（HBO）治疗组40例，采用放免法测定各组患者于入院时即刻、24h．7d时血清细胞因子水平，并与30例健康对照组比较。结果ACOP入院时即刻TNF—α、IL-6、IL-8水平明显高于对照组（P〈0.01），24h后开始下降，至第7天时降至最低水平，但仍高于对照组（P〈0．05）。中毒后IL—10水平减低（P〉0．05），24h后开始升高（P〈0．05），7d时明显升高（P〈0．01）。HBO组各种血清细胞因子水平变化较NBO组更为明显（P〈0．05）。结论TNF-α、IL-6、IL-8、IL-10参与ACOP的病理过程，HBO可减轻ACOP后的免疫病理损伤。     

白细胞介素-6、白细胞介素-8及肿瘤坏死因子-α与脑出血患者出血量及预后的关系

目的研究脑出血患者血清白细胞介素-6（IL-6）、白细胞介素-8（IL-8）和肿瘤坏死因子-α（TNF—α）含量的变化及其与出血量、预后的关系。方法分别测定60例脑出血患者和20例健康体检者（对照组）血清IL-6、IL-8及TNF-α含量,观察比较脑出血患者在不同出血量（小、中、大量）时的含量变化,同时对出院者进行神经功能缺损评分（NDS）评定,观察IL-6、IL-8及TNF—α含量水平对脑出血预后生活质量的影响。结果脑出血组血清IL-6、IL-8及TNF—α含量显著高于对照组,差异有统计学意义（P〈0．05）;脑出血患者大量、中量组的IL-6、IL-8及TNF—α含量显著高于小量组（P〈0．05）;大量组的IL-6、IL-8及TNF—α含量显著高于中量组（P〈0．05）。脑出血患者血清中IL-6、IL-8及TNF-α含量越高,NDS越高。结论血清中IL-6、IL-8及TNF-α含量的测定可能反映脑出血患者的出血量,还可以预测患者的预后。     

蛙血清去蛋白提取物对大鼠脑缺血再灌注损伤的保护作用

目的探讨蛙血清去蛋白提取物对大鼠脑缺血再灌注损伤的保护作用及其机制。方法将SD雄性大鼠72只,随机分为6组：假手术组、模型组、奥德金组（20mg/kg）和蛙血清去蛋白提取物高（90mg／kg）、中（30mg／kg））、低（10mg／kg）剂量组。采用线栓法栓塞大鼠大脑中动脉建立大鼠脑缺血再灌注损伤模型,从术前7d开始,每天1次,造模当天于手术前1h给药,再灌注后1h给药1次。分别用化学比色法测定脑组织超氧化物歧化酶（SOD）、丙二醛（MDA）和谷胱甘肽过氧化物酶（GSH-Px）含量;酶联免疫吸附法（ELISA）法测定肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）含量;原子吸收分光光度法测定Ca卦含量;干湿重法测定脑组织含水量。结果模型组大鼠脑组织SOD、GSH-Px含量明显降低,MDA、TNF-α、IL-1β、Ca。’含量和含水量明显升高（P〈0．01）;蛙血清去蛋白提取物能降低脑组织MDA、TNF-α、IL-1β、Ca^2＋含量和含水量,升高SOD、GSH-Px含量（P〈0．05）。结论蛙血清去蛋白提取物防治脑缺血再灌注损伤的机制与提高抗氧化能力、抑制炎症起始因子、阻滞Ca^2＋超载有关。     

病毒性心肌炎患者血清TNF-α、IL-6和IL-8的变化

目的检测病毒性心肌炎（VMC）患者血清TNF-α、IL-6和IL-8的水平变化,探讨其在临床的意义。方法采用酶联免疫吸附试验（ELISA）法测定48例病毒性心肌炎急性期和恢复期患者血清TNF-α、IL-6和IL-8水平,与正常对照组进行比较。结果VMC患者急性期血清TNF-α、IL-6、IL-8均显著高于恢复期及正常对照组（P〈0．01）,而恢复期与正常对照组比较差异无显著性意义（P〉0．05）,且VMC患者急性期血清CK-MB与TNF-α、IL-6、IL-8均呈良好的正相关（P〈0．01）。结论VMC患者血清TNF-α、IL-6、IL-8的检测对VMC患者病情评估和预后判断具有重要意义。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.