准分子激光角膜切削术治疗近视散光

目的：评价准分子激光角膜切削术（ＰＲＫ）治疗近视散光的临床效果。方法：对２６０眼近视散光用ＶＩＳＸ２０／２０准许分子激光系统治疗并随访一年。结果：术前近视散光－０．２５￣－４．００Ｄ，平均－１．００±０．６２Ｄ；术后６月为－０．０７±０．４０Ｄ；术后１年为－０．０６±０．３９Ｄ。术后较术前散光度明显降低（ｐ〈０．０５）。术后１年较术后６月散光理低（ｐ〉０．０５）。无明显回退现象。结论：ＰＲＫＣＦ     

准分子激光角膜切削术治疗近视散光

目的：评价准分子激光角膜切削术（ＰＲＫ）治疗近视散光的临床效果。方法：对２６０眼近视散光用ＶＩＳＸ２０／２０准分子激光系统治疗并随访一年。结果：术前近视散光为－０．２５～－４．００Ｄ，平均－１．００±０．６２Ｄ；术后６月为－０．０７±０．４０Ｄ；术后１年为－０．０６±０．３９Ｄ。术后较术前散光度明显降低（Ｐ＜０．０５）。术后１年较术后６月散光度更低（Ｐ＞０．０５）。无明显回退现象。结论：ＰＲＫ对近视散光具有理想的治疗效果。     

准分子激光治疗近视眼的初步报告

目的：报告采用准分子激光切削术治疗近视散光５５例１０４只眼的初步疗效结果。方法：应用ＮＩＤＥＫＥＣ—５０００准分子激光系统对５５例近视散光进行手术治疗。１０４只眼按近视程度分组：Ａ组（≤—６．００Ｄ），Ｂ组（－６．２５Ｄ～－１０．００Ｄ），Ｃ组（＞－１０．２５Ｄ）。结果：术后观察６个月，各组平均裸眼视力均显著提高，实际矫正度在预测矫正度±１．００Ｄ范围内者分别占９７．５０％、９１．３０％及７７．７８％，超高度近视与中、高度近视相比Ｐ＞０．０５差异无显著性。结论：ＰＲＫ技术不仅对低、中度近视，而且对－１０．２５Ｄ～－１３．００Ｄ高度近视也具有良好的治疗效果。     

准分子激光屈光性角膜切削术治疗近视的疗效分析

目的 评价准分子激光屈光性角膜切削术（ＰＲＫ）治疗近视的疗效。方法 应用ＳｃｈｗｉｎｄｋｅｒａｔｏｍＦ型准分子激光机对近视屈光度为－１．７５￣＝１２．００Ｄ的患者３０２眼行ＰＲＫ治疗。按术前球镜屈光度分为两组：Ⅰ组为－１．７５￣－６．００Ｄ（１８６眼）,Ⅱ组为－６．２５￣－１２．００Ｄ（１１６眼）。随访一年以上结果 术后１２个月,裸眼视力≥０．５者在Ⅰ、Ⅱ组中分别为９６．７％、９０．５％,≥１．０     

准分子激光屈光性角膜切削术治疗轻,中度近视眼

评价准分子激光屈光性角膜切削术治疗轻，中度近视眼的临床效果。对在我院接受ＰＲＫ治疗的８１只轻，中度近视及近视散光眼的术后半年随访结果进行统计分析。术前近视度范围为－１．００－６．００Ｄ，其中轻度近视占１６．０％，中度近视占８４．０％，单纯近视占５６．８％，复性近视散光占４３．２％。     

准分子激光原位角膜磨镶术治疗高度近视

目的：评估准分子激光原位角膜磨镶术（ＬＡＳＩＫ）治疗高度近视的安全性,有效性、稳定性及预测性。方法：采用ＳＣＭＤ气动式角膜切割刀及ＳＣＨＷＩＮＤ ＫＥＲＡＴＯＮ－Ｆ型准分子激光系统,对高度近视（等值球镜－６．２５Ｄ￣－３０．００Ｄ,平均（－１２．５０±５．３９）Ｄ）１０４例１６４只眼行ＬＡＳＩＫ手术,其中屈光度－６．２５Ｄ￣－９．５０Ｄ（Ａ组）７４只眼;屈光度－１０．１３Ｄ￣－１５．００Ｄ（Ｂ组）     

准分子激光屈光性角膜切削术治疗近视眼的远期效果分析

目的：评价准分子激光屈光性角膜切削术（ＰＲＫ）治疗不同程度近视眼的远期疗效。方法：应用ＶＩＳＸ２０／２０型准分子激光机对２５８只不同程度的近视眼进行ＰＲＫ治疗,对３～５年随 是行统计分析,按术前近视度分两组,Ａ组－１．００～－６．００Ｄ（等球镜,下同）１１６只眼,Ｂ组－６．２５～－１２．００Ｄ１４２只眼。结果：经ＰＲＫ治疗后,Ａ组裸眼视力≥１．０占８６．２％,≥０．５占９７．４％,剩余屈光度为（－     

准分子激光角膜切削术治疗近视179例

使用准分子激光光学角膜削切术（ＰＲＫ）治疗３２６眼（１７９例）近视度数在－２．２５～ －２０． ００ Ｄ的近视患者，并进行了 ６～１２个月的随访。结果：术前视力 ０．０１～０． ２，术后裸眼视力≥ ０．５者占８９．２６％，其中轻度近视为１００％，中度为９７．３％，重度为８０．０％。角膜混浊均≤Ⅱ级。术 后平均屈光度±１． ００ Ｄ。结果提示 ＰＲＫ是治疗近视的有效方法。     

准分子激光角膜原位磨镶术治疗近视临床报告

目的 评价准分子激光角膜原位磨镶术（ＬＡＳＩＫ）治疗中、高度近视安全性。方法 对２２例（４１眼）中、高义近视行ＬＡＳＩＫ手术,其屈光度（等值球镜）－２．５０Ｄ－－１０．００Ｄ,平均－６．５７±２．０１Ｄ,散光≤－３．５０Ｄ,术后１、３个月复查视力、屈光度、角膜地形图、角膜上皮及角膜层间混浊（Ｈａｚｅ）等并发症情况。结果 术后１月平均眼视力１．０２±０．０９８,其中≥１．０者达６５．８５％,矫正视力     

准分子激光角膜切削治疗近视661只眼近期疗效分析

目的：探讨准分子激光角膜切削术（ＰＲＫ）治疗近视眼的近期疗效。方法：对６６１只不同度数的患眼经ＰＲＫ术后疗效的分析。结果：视力恢复达１．０以上的Ⅰ组（≤－３．００Ｄ）７０眼，占总数７５眼的９３．３％，Ⅱ组（≤－６．００Ｄ）２３３眼，占总数２６７眼的８７．３％，Ⅲ组（≤－１２．００Ｄ）１７３只眼，占总数２６８眼的６４．６％，Ⅳ组（〉－１２．００Ｄ）３眼，占总数５１眼的５．８％。剩余屈光度：≤－０．５     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.