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1.
G. K. Özgür B. Pişkin A. Sivrikaya N. Gacar S. Erkul 《International urology and nephrology》1990,22(5):461-466
It has been previously shown that unilateral testicular torsion can cause disruptive anatomic changes in the contralateral
testis in rats [1]. In this experimental study plasma and urine prostaglandin E2 levels were studied correlatively with testicular
histopathology in acute testicular torsion cases. As a result of this study, necrobiotic morphologic alterations causing testis
necrosis and significant increase in plasma prostaglandin E2 levels were observed. Contralateral testicular histology was
analyzed in all dogs. None of them showed abnormal tubular architecture. 相似文献
2.
一侧睾丸扭转对对侧睾丸组织发育的影响 总被引:4,自引:0,他引:4
目的:研究一侧睾丸扭转以后对对侧睾丸组织的影响。方法:以大鼠为研究对象,按扭转时间及药物应用情况进行分组。在一侧睾丸扭转以后2个月,观察对侧睾丸曲细精管的变化。结果:一侧睾丸扭转2h以内,对侧睾丸曲细精管管腔和生精上皮增生;扭转6 h以后,曲细精管管腔和生精上皮萎缩;扭转6h以内应用别嘌呤醇能缓解对侧病变。结论:一侧睾丸扭转可以使对侧睾丸组织发育发生病理改变。 相似文献
3.
On unilateral testicular and epididymal torsion: no effect on the contralateral testis 总被引:3,自引:0,他引:3
T T Turner 《The Journal of urology》1987,138(5):1285-1290
It is often stated that unilateral testicular torsion results in damage to the contralateral testis; however, there are a growing number of experimental and clinical papers which suggest this is not so. Conflicting results from experimental studies confuse the issue and may be due, among other things, to some specifics of the experimental model. In the present paper, we have examined bilateral rat testes 30 and 60 days after 720 degrees torsion to determine 1) the effect of unilateral testicular torsion with and without the inclusion of epididymal torsion, 2) the effect of relatively chronic torsion (24 hr., 10 day) versus relatively acute torsion (two hr., four hr.), and 3) the effect of establishing the model using scrotal surgery versus using an abdominal approach. Bilateral testicular histology, testis wt. (gm.), cauda epididymal sperm concentrations (sp./ml.), and cauda sperm motility scores (0-4) were examined. Ipsilateral testicular torsion or testicular plus epididymal torsion of two hr. or four hr. duration significantly reduced (p less than .05) ipsilateral testis weights, sperm concentrations, and motility scores, and disrupted normal tissue histology. Contralateral testicles were not altered. Epididymal ischemia alone produced no significant ipsilateral or contralateral effects. Chronic torsion (one day, 10 days) also destroyed ipsilateral testis function without altering the contralateral testicles. The occult cryptorchidism associated with the scrotal approach to establishing the torsion model had no effect on contralateral testicles. In no group, using either Lewis rats or Sprague-Dawley rats, were contralateral testicles altered by unilateral testicular torsion. These results plus recent clinical reports indicate that contralateral testicular damage due to ipsilateral torsion is hardly a proven phenomenon, let alone a significant factor contributing to male infertility. 相似文献
4.
大鼠单侧睾丸扭转复位后对对侧睾丸的影响 总被引:1,自引:1,他引:0
目的研究大鼠单侧睾丸扭转复位后对对侧睾丸的影响。方法16只成年健康SD雄性大鼠随机分为实验组(n=8)和对照组(n=8);建立单侧睾丸扭转复位模型。术后30d取扭转对侧睾丸,采用原位缺口末端标记法(TUNEL)检测生殖细胞凋亡,光镜下计数精子数。结果与对照组相比,实验组对侧的睾丸重量和日产精子量都有显著性差异(P<0.05),实验组生殖细胞凋亡显著增多(P<0.01)。结论大鼠单侧睾丸扭转后,对侧睾丸生殖细胞凋亡增多可能是导致不育的原因之一。 相似文献
5.
This study was instituted to evaluate the effect of unilateral testicular torsion on contralateral testicular histology and the prevention of this effect by prednisolone. Fifty Swiss albino rats were equally divided into 5 groups. In group 1, it was observed that, due to torsion, the mean seminiferous tubular diameter and percentage of spermatogenetic activity of the contralateral testes were reduced and an inflammatory reaction was also noted. In group 2, detorsion increased the above-mentioned damage, and in group 3, orchiectomy failed to prevent it. In group 4, it was seen that prednisolone slightly increased the mean percentage of spermatogenetic activity and produced proliferation of the Leydig cells in the intact testicle. In group 5, when prednisolone was injected just after torsion, no damage to the contralateral testes appeared. It has been thought that damage to the contralateral testes may arise from an autoimmune mechanism and prednisolone appeared to be very helpful in preventing damage by immunologic suppression. 相似文献
6.
Unilateral testicular torsion: protective effect of verapamil on contralateral testicular histology. 总被引:2,自引:0,他引:2
In this experimental study, it was our aim to reduce the effects of ischemic insults to the contralateral testicle after unilateral testicular torsion. The protective effect of a calcium channel blocking agent (verapamil) on the histology and the tubular diameter of contralateral testicle was evaluated. Following a definite period of unilateral testicular torsion (i.e., 4 h), the protective effect of this specific medication was evaluated both after detorsion and orchiectomy procedures. The results of our study demonstrated the protective effect of verapamil on both parameters, especially in animals undergoing orchiectomy. The majority of the specimens demonstrated normal histologic findings together with preserved tubular structures after a 1-week period under verapamil medication. 相似文献
7.
M Sade S Amato C Buyuksu S Mertan M S Canda N Kaplanoglu 《British journal of urology》1988,62(1):69-71
In an attempt to investigate the effect of testicular torsion and various forms of treatment on the contralateral testis, an experimental study on rats was undertaken. The first group comprised control animals. In the second group the left testes were twisted 720 degrees and the right testes were removed 4 weeks later for histopathological examination. In the third group the rats were subjected to a left detorsion procedure 24 h after torsion, while in the fourth group cortisone treatment was added to the above procedure. The fifth group consisted of rats which had undergone left orchiectomy 24 h after torsion and the sixth group had cortisone treatment plus orchiectomy after torsion. Cortisone treatment was started 24 h after testicular torsion and continued for 4 weeks. Histopathological examination of the contralateral testes which were removed 4 weeks later showed that either orchiectomy plus cortisone or detorsion plus cortisone was more successful than other forms of treatment. 相似文献
8.
I Madgar B Lunenfeld S Mashiach B Goldwasser R Weissenberg 《Archives of andrology》1987,19(3):237-241
The effect of unilateral testicular torsion on the contralateral testis and the fertility rate was studied in Charles River adult rats. Animals were divided into groups that underwent a sham operation or torsion and ligation of the left testicular vessels followed by orchiectomy after 24 h, orchiectomy after 48 h, release of the ligature after 24 h, release of the ligature after 48 h, and no further treatment following ligation. Another group of animals underwent unilateral orchiectomy. After 8 weeks animals were allowed to mate and were sacrificed 2 weeks later. The results did not point to either histological alterations in the contralateral testis or impairment of fertility in any group of treatment compared with the control. 相似文献
9.
Paredes Esteban RM Ramírez Chamond R Carracedo Añón J Salas Molina J Hervas Rodríguez J Garrido Pérez JI Ocaña Losa JM 《Cirugía pediátrica : organo oficial de la Sociedad Espa?ola de Cirugía Pediátrica》1999,12(4):152-154
The purpose of this project is to determine the lesion in contralateral testis after unilateral testicular torsion. For that is reproduced the lesion in an experimental model animal using 35-45 days old Wistar rats. The study of the contralateral testis is performed 30-35 days after lesion, once orchiectomy with histopathological and ultrastructural analysis, was made. We observed zonal tubular atrophy, alteration of spermatogenesis, abnormal formation of spermatozoa and death cellular by apoptosis. 相似文献
10.
Capsaicin effectively prevents apoptosis in the contralateral testis after ipsilateral testicular torsion 总被引:1,自引:0,他引:1
Sarioglu-Buke A Erdem S Gedikoglu G Bingol-Kologlu M Tanyel FC 《BJU international》2001,88(7):787-789
OBJECTIVE: To evaluate the effect of afferent nerve blockage by administration of capsaicin on apoptotic changes in the contralateral testis in rats undergoing ipsilateral testicular torsion. MATERIALS AND METHODS: Twenty male albino rats were randomly divided into four groups. In groups 1 and 2, rats underwent a sham operation and testicular torsion, respectively, after the intraperitoneal administration of 0.9% NaCl. Similarly, in groups 3 and 4 the rats underwent a sham operation and testicular torsion, respectively, after an intraperitoneal capsaicin injection. The testes were untwisted 24 h later and the contralateral testes harvested. Apoptosis was assessed in paraffin-embedded sections stained for nuclear DNA fragmentation. Fifteen cells were counted in each seminiferous tubule and the apoptotic cells recorded. A score was calculated for each group and the results compared using the Kruskal-Wallis analysis of variance and Mann Whitney U-tests, with P<0.05 considered to be significant. RESULTS: The mean apoptotic score of group 2 was significantly higher than that of the other groups. There was no difference between the apoptotic scores of groups 1 and 3, 1 and 4, and 3 and 4. CONCLUSION: Capsaicin effectively prevented apoptosis in the contralateral testes of rats that had undergone testicular torsion. 相似文献
11.
Experimental testicular torsion: time course of the effect on the contralateral testicular histology
Summary Since fertility is decreased in patients with unilateral torsion, a detrimental influence of the damaged tesis on the contralateral side has been postulated. In previous studies, we could not detect such an effect 2 months after the application of torsion. The present study was designed to search for an early defect that may be followed by regeneration as well as for a defect that appears only later. At 2 weeks, 4 months and 7 months after unilateral testicular torsion and sham operation, respectively, in rats, both testes were examined. By means of morphometry, the different testicular components of the contralateral testes were quantified, but neither early nor late damage could be detected. Therefore, despite some other conflicting studies, we do not believe that contralateral testicular damage necessarily occurs after torsion. The decreased fertility may be explained by an immunologic attack on the spermatozoa on their way out of the genital tract. 相似文献
12.
13.
一氧化氮在一侧睾丸扭转对侧睾丸损伤中的作用 总被引:7,自引:1,他引:6
目的 研究总抗氧化能力(T-AOC)和一氧化氮(NO)在一侧睾丸扭转对侧睾丸损伤中的作用。方法 SD雄性大白鼠建立左侧睾丸扭转模型,于扭转后6h再分为扭转睾丸复位及切除组,分别于术后1h、1d、1周、2周和4周处死4—5只,取出睾丸用于一氧化氮合酶(NOS)活性、NO、T-AOC及细胞凋亡的检查。结果 UTT复位后对侧睾丸组织NOS活性、NO含量明显升高,T—AOC显著降低。结论 NO过量产生及T-AOC的下降是UTT对侧睾丸损伤的重要原因。 相似文献
14.
Paredes Esteban RM Ramírez Chamond R Carracedo Añón J Rodríguez Portillo M 《Cirugía pediátrica : organo oficial de la Sociedad Espa?ola de Cirugía Pediátrica》2003,16(1):4-7
The role the FAS and BCL-2 in the apoptosis of testicular cells in the contralateral testis after unilateral testicular torsion, was investigated. We compared with control group. These experiments were performed in male Wistar rats prepuberal old. FAS and BCL-2 determination is realized in cells cultures of contralateral testis. Flow cytometry and immunohistochemistry studies, using a FAS and BCL-2 specific monoclonal antibody, were utilized to value FAS y BCL-2 expression on testiculaires cells following unilateral testicular torsion. We observed an increase of expression of FAS and decrease of BCL-2 in the contralateral testis in comparison with control group. The present results may indicate that the expression of this molecules is implicated in cellular apoptosis. 相似文献
15.
16.
Undescended testis and testicular torsion 总被引:1,自引:0,他引:1
C A Sheldon 《The Surgical clinics of North America》1985,65(5):1303-1329
This article reviews the concepts basic to the management of the undescended testis and testicular torsion and provides specific guidelines for the management of the many variations of these entities. 相似文献
17.
G. Karagüzel F. C. Tanyel N. Büyükpamukçu A. Hiçsönmez G. Gedikoğlu 《Urological research》1994,22(2):115-117
Although deteriorating effects of unilateral spermatic cord torsion are generally accepted, the mechanism remains controversial. An experimental study was performed to evaluate the necessity of testicular and spermatogenetic material for contralateral testicular deterioration following unilateral spermatic cord torsion in rats. The animals were allocated to four groups: control, spermatic cord torsion, subepididymal orchiectomy, and spermatic cord torsion 14 days after subepididymal orchiectomy. The testes were removed on the 14th days and mean seminiferous tubular diameters and mean testicular biopsy scores were determined. Although contralateral testicular deterioration was more pronounced in the presence of testicular tissue, the absence of testicular tissue and/or spermatogenetic material did not prevent its occurrence. This is highly suggestive that autoimmune mechanism does not play a role in contralateral testicular damage following unilateral spermatic cord torsion. 相似文献
18.
19.
The protective effects of nitric oxide on the contralateral testis in prepubertal rats with unilateral testicular torsion 总被引:6,自引:0,他引:6
OBJECTIVE: To investigate histological changes in the contralateral testis of rats with unilateral testicular torsion and the protective effects of nitric oxide (NO) on possible damage. MATERIAL AND METHODS: Twenty-eight prepubertal male Sprague-Dawley rats were divided into four equal groups. Group 1 underwent a sham operation of the right testis under general anaesthesia. Group 2 underwent a similar operation but the right testis was rotated 720 degrees clockwise for 6 h, maintained by fixing the testis to the scrotum, and saline infused during the procedure. Group 3 underwent similar torsion but L-arginine methyl ester (a precursor of NO) was infused during the procedure. In Group 4, NG-nitro-L-arginine-methyl ester, a NO synthase inhibitor, was infused separately during the administration of L-arginine methyl ester and torsion. All the left (untwisted) testes were removed from rats 21 days after surgery and evaluated histologically, assessing seminiferous tubule diameter, loss of sperm and spermatids, loss of germ cell layers, disarray of germ cell layers, rupture of tubules, Leydig cell proliferation and reaction in the ruptured tubules, and oedema. RESULTS: There was a significant difference in the indicators of histological damage between groups 2 and 4 and groups 1 and 3, except for the Leydig cell reaction in the ruptured tubules and oedema. The damage was significantly less in group 3 than in groups 2 and 4. CONCLUSION: These results suggest that long-term histopathological changes in the contralateral testes are important after unilateral testicular torsion and that NO has a protective effect on the contralateral testis. 相似文献
20.
Immunohistopathology of the contralateral testis of rats undergoing experimental torsion of the spermatic cord 总被引:11,自引:0,他引:11
Aim:To evaluate the immunohistopathological changes in the contralateral testis of rats after an experimental sper-matic cord torsion.Methods:Male Sprague-Dawley rats of 45-50 days old were subjected to a 720° unilateralspermatic cord torsion for 10,30 and 80 days(experimental group,E),respectively or sham operation(controlgroup,C).Histopathology of the contralateral testis as well as germ cell apoptosis were studied using the TerminalDeoxynucleotidyl Transferase Biotin-dUTP Nick End Labeling(TUNEL)technique.The number of testicularlymphocytes,mast cells and macrophages,and the expression of tumor necrosis factor-α(TNF-α)and its receptor(TNFR1)in testicular cells of the contralateral testis were quantified by histochemistry and immunohistochemistry.TNF-α concentration in testicular fluid was determined by ELISA.Results:In the contralateral testis of rats fromthe E group,the maximal degree of damage of the germinal epithelium was seen 30 days after torsion.At this time weobserved in the E group vs.the C group increases:(i)the number of testicular T-lymphocytes;(ii)the number oftesticular mast cells and macrophages;(iii)the percentage of macrophages expressing TNF-α:(iv)TNF-α concen-tration in testicular fluid;(v)the number of apoptotic germ cells;and(vi)the number of TNFR1~ germ cells.Conclusion:Experimental spermatic cord torsion induces,in the contralateral testis,a focal damage of seminiferous tubulescharacterized by apoptosis and sloughing of germ cells.Results suggest humoral and cellular immune mediatedtesticular cell damage in which macrophages and mast cells seem to be involved in the induction of germ cell apoptosisthrough the TNF-α/TNFR1 system and in the modulation of the inflammatory process.(Asian J Andro12006 Sep;8:576-583) 相似文献