孤立性纤维性肿瘤的临床病理免疫组化分析

目的分析孤立性纤维性肿瘤临床病理免疫组化特点。方法选取孤立性纤维性肿瘤患者共30例,回顾其临床、病理及免疫组织化学资料。结果本次研究中所有患者发生部位包括鼻腔、口腔、纵隔、眼眶以及腮腺等。临床表现为肿块引起的压迫症。病理学改变:肿瘤大小1.5~20 cm,边界清楚;瘤细胞呈短梭形,细胞质红染,核分裂象少见,可见大量胶原纤维。其中7例vimentin阳性,16例CD34阳性,1例CD99阳性,3例SMA阳性,3例S-100阳性。结论孤立性纤维性肿瘤能够在患者身上多个部位发生,结合病理镜下表现和临床特点及免疫组化能够做出较为准确的诊断,低度恶性肿瘤情况较少,表现为远处转移或局部复发。     

腹膜后孤立性纤维性肿瘤 4例临床病理分析

目的探讨腹膜后孤立性纤维性肿瘤( Solitary Fibrous Tumor,SFT)的临床病理特征、免疫表型、诊断与鉴别诊断要点、治疗及预后。方法收集 2015年 1月至 2018年 12月就诊于武汉市第三医院的 4例腹膜后孤立性纤维性肿瘤进行组织病理学观察及免疫组织化学检测,总结其临床及影像学表现、组织学形态、免疫表型特征和临床预后。免疫组织化学采用 EnVision法检测内皮细胞标记物( CD34)、 B淋巴细胞瘤 ?2基因( Bcl?2)、 CD99分子重组蛋白( CD99)、信号转导和转录活化因子 6(STAT6)及其他相关标志物。并结合相关文献进行讨论分析。结果 4例腹膜后孤立性纤维性肿瘤均是因“无症状腹膜后肿物”就诊,病理特征为肿瘤由梭形、短梭形细胞构成,排列无序并与胶原纤维混杂,肿瘤中血管丰富,部分形成血管外皮瘤样区域,免疫组化 STAT6(+)、 CD34(+)、 Bcl?2(+)、 CD99(+)、人 S100蛋白( S?100)(-)、酪氨酸激酶生长因子受体蛋白( CD117)(-)、 Discovered on GIST?1(Dog?1)(-)、细胞角蛋白( CK)(-)、结蛋白( Desmin)(-)、黑色素瘤抗体( HMB45)(-)、上皮膜抗原( EMA)(-)。 1例失访,余均未复发。结论腹膜后孤立性纤维性肿瘤为中间型肿瘤,临床上比较少见,难以通过影像学检查确诊,缺乏术前诊断依据,极易误诊、漏诊,须依据病理诊断,免疫组化进行诊断与鉴别诊断。该病临床上预后较好,完整切除后罕见复发,但需长期随访。     

孤立性纤维性肿瘤的临床病理分析

目的探讨孤立性纤维性肿瘤的临床病理特征,诊断及鉴别诊断。方法对1例发生在乳腺的孤立性纤维性肿瘤进行组织学观察和免疫组化检测,并复习相关文献。结果肿瘤由梭形细胞构成,形成细胞稀少区和富于细胞区,两者交替分布,其间有粗大的瘢痕样玻璃变性的胶原纤维,免疫组化bcl-2和CD34阳性表达,CK为阴性。结论孤立性纤维性肿瘤为中间型肿瘤,完整切除后很少复发,但需长期随访。     

椎管内原发性孤立性纤维性肿瘤临床病理观察(附2例报告)

目的探讨椎管内孤立性纤维性肿瘤的临床病理特征、诊断和鉴别诊断,以期提高对该肿瘤的诊断水平。方法复习2例椎管内原发性孤立性纤维性肿瘤的临床资料,并观察其组织学特征和免疫组化标记。结果2例患者男、女各1例,年龄分别为23岁和32岁,临床表现为局部神经压迫症状。MRI示椎管内髓外硬膜内占位。组织学特征为梭形、卵圆形细胞呈束状、波浪状或旋涡状排列,富于胶原纤维及伴有分支状薄壁血管。细胞未见异型性和核分裂像。免疫组化示瘤细胞Vimentin( ),CD34( ),CD99( ),Bcl-2( ),AACT(-),Actin(-),S-100(-),EMA(-),GFAP(-),CD68(-),CD117(-),SMA(-),NF(-)。结论椎管内原发性孤立性纤维性肿瘤是一种罕见的肿瘤,诊断主要依靠病理形态学及免疫组化,并应与椎管内的其他梭形细胞肿瘤鉴别。     

脑膜孤立性纤维性肿瘤临床病理观察

目的 探讨脑膜孤立性纤维性肿瘤的临床病理学特点、免疫组化特征及鉴别诊断.方法 对1例脑膜孤立性纤维性肿瘤并发生腰椎转移患者的手术切除标本进行组织学观察,免疫组化进行CD34、Bcl-2、CD99、vimentin、CKpan、GFAP、EMA、CD117、S-100、Ki67染色及随访,并复习相关文献.结果 转移瘤标本肉眼所见为不规则的碎骨及软组织,大小2 am×2 cm×3 cm,表面欠光滑,切面暗灰色、实性、鱼肉样.镜下检查:肿瘤由交替性分布的富于细胞和稀疏细胞区组成,梭形细胞呈束状、漩涡状或不规则状排列,部分与胶原纤维混杂,部分呈血管外皮瘤样结构.上皮样细胞有的围绕血管周围.细胞间细条索状、粗绳索样或瘢痕样胶原纤维不规则性或石棉样的胶原物质沉积.瘤细胞CD34、Bcl-2、CD99、Vimentin均为阳性,CKpan、GFAP、EMA、CD117、S-100蛋白均为阴性,Ki67增殖指数<5%.结论 脑膜孤立性纤维性肿瘤罕见,易复发和转移.需要与其他多种梭形细胞肿瘤鉴别诊断,其组织学特点和免疫组化可以帮助鉴别.其生物学行为难以预测,必须进行长期随访.

Abstract：

Objective To explore the clinicalpathological features,immunohistoehemieal characteristics and differential diagnosis of solitary fibrous tumor(SFT) of the meningeal.Methods A case suffering from SFT of the meningeal was examined with light microscopy and immunohistochemistry,including CD34,Bcl-2,CD99,Vim-entin,CKpan,GFAP,EMA,CD117,S-100 and Ki67,and related literatures were reviewed.Resuits The patient was a 32-year-old man,who was found neoplasm in IA vertebrae after removing the solitary fibrous tumors of the right temporal meningeal.Grossly,the tumor was composed of irregular bone fragments and soft tissue with dimension 2 cm×2 cm x 3 cm and dark gray.solid,fish-like on cut surface.Microscopically,the tumor was composed of bland spindle shaped cells and dense collagenous bands,with fascicular,storiform or haphazard arrangements.Focal areas showed a haemangiopericytoma like structure.Immunohistochemically,the tumor cells were positive for CD34.Bcl-2,CD99 and Vimentin,but negative for CKpan,GFAP,EMA,CD117,S-100,Ki-67 index was less than 5%.Condusious SFI"is a very rare tumor in meningeal.which often manifests local recurrence and metastasis.It should be differentiated from a variety of spindle cell tumors.Diagnosis is based on hisitologieal features supplemented with immunohistochemical findings.It's very difficult to predict the biological behavior,which requires a long-term follow-up.     

胃肠道间叶源性肿瘤的病理及免疫组化研究

目的探讨胃肠道间叶源性肿瘤病理及免疫组化情况。方法我院2012年1月至2013年12月120例胃肠道间叶源性肿瘤患者,以免疫组化SP法测定CD117、CD34、Vimetin、S-100、SMA、Bcl-2表达。结果 120例胃肠道间叶源性肿瘤中有85%为胃肠道间质瘤,平滑肌肿瘤8.3%,神经鞘瘤1.7%。102例胃肠道间质瘤中CD117表达率90.2%,CD34表达率80.4%,Vimetin表达率86.3%,Bcl-2表达率74.5%,S-100表达率11.9%。结论胃肠道间叶源性肿瘤主要为胃肠道间质瘤,进行胃肠道间叶源性肿瘤临床诊断需与免疫组化相结合,方可得到准确诊断结果。胃肠道间叶源性肿瘤临床诊断时应联合应用一组免疫组化抗体方法,CD117和CD34属于诊断中最具有敏感性有效性的免疫组化标记。     

孤立性纤维性肿瘤9例临床病理分析

目的探讨孤立性纤维性肿瘤（solitary fibrous tumour,SFT）的临床病理和免疫组化特征。方法对9例孤立性纤维性肿瘤的临床、病理表现和免疫组化检查结果进行分析,并复习文献。结果肿瘤位于肺、前上纵隔、腹壁、盆腔、颈前、软腭及眼睑。临床表现为局部无痛性肿块及压迫症状。组织学特征：肿瘤细胞密集区及稀少区交替出现,细胞呈短梭形及卵圆形,排列方式多样。间质见粗细不等的胶原纤维及较多薄壁血管。免疫组织化学结果：CD99、Bcl-2及波形蛋白（Vimentin）均阳性,CD34阳性（8/9）,平滑肌肌动蛋白（SMA）阳性（3/9）,S-100阳性（1/9）,结蛋白（Desmin）不表达。结论 SFT的病理诊断主要依赖组织学特点和免疫组化染色结果。SFT多为良性,但其生物学行为难以预料,应长期随访。     

所谓肺硬化性血管瘤的临床病理及免疫表型研究

目的探讨肺硬化性血管瘤的临床病理特征、免疫表型特点及组织来源。方法应用免疫组化检测26例肺硬化性血管瘤中TTF-1、EMA、Vim、CD34、CD31、CD68、Ⅷ因子、SMA、CK5/6、CK-L、CK-H、CgA、PR及ER的表达,观察病理组织学特征并进行随访。结果肺硬化性血管瘤细胞成分及组织结构复杂,部分病例镜下结构类似类癌、孤立性纤维性肿瘤及非黏液型支气管肺泡癌。肿瘤实质由上皮样细胞及卵圆形细胞构成,组织结构可见实性区、乳头区、血管瘤样区及硬化区。间质可发生脂肪化生及骨化。免疫组化示：两种细胞均表达TTF及EMA,上皮样细胞表达CK、卵圆形细胞表达Vimentin、ER、PR,个别表达Syn。随访未见因肿瘤死亡的病例。结论肺硬化性血管瘤可能起源于呼吸道的原始多潜能干细胞,具有向肺泡Ⅱ型细胞分化的特点。病理诊断中需与类癌、孤立性纤维性肿瘤及非黏液型支气管肺泡癌鉴别。虽有个别病例出现淋巴结转移,但临床预后良好。PR的表达可能与女性的高发病率有关。     

胸膜孤立性纤维性肿瘤的诊断及外科治疗

目的 探讨胸膜孤立性纤维性肿瘤(SFTP)的诊断及治疗方法.方法 回顾9例胸腔肿瘤手术切除后病理确诊为SFTP的病例,就其诊断和治疗方法进行分析.结果 9例中,6例有咳嗽胸闷等症状,1例伴有严重低血糖,2例无明显症状体检发现.CT检查表现为包膜完整光滑的类圆形阴影,可伴有胸腔积液.术中探查肿瘤8例位于脏层胸膜,1例位于壁层胸膜.手术完整切除肿瘤,合并肺楔形切除1例,肋骨部分切除1例.肿瘤最大直径3～30 cm.镜下SFTP由大量梭形细胞构成,间质含有大量粗细不等的胶原纤维.免疫组化结果CD34阳性率为100%,Bcl-2阳性率为83.3%.结论 早期SFTP大多无明显症状,诊断比较困难.压迫症状及低血糖症状的出现,应当高度怀疑SFTP.CT检在是重要的诊断手段.手术切除肿瘤是治疗SFTP的最好方法.确诊有赖于病理及免疫组织化学检查.     

孤立性纤维性肿瘤2例临床病理分析

目的探讨孤立性纤维性肿瘤（SFT）的临床病理学特征及诊断、鉴别诊断。方法对2例SFT行临床病理学分析及免疫组化研究。结果镜下见肿瘤由增生的梭形细胞或卵圆形细胞和胶原纤维组成,形成交替分布的丰富细胞区和疏松细胞区,并可见血管外皮瘤样结构,肿瘤细胞间血管丰富。免疫组织示CD34、CD99、Vimentin均（＋）,S-100、Desmin、SMA均（-）。结论孤立性纤维性肿瘤大部分为良性,少数可为恶性。完整手术切除是良好的预后因素。其诊断与鉴别诊断主要依据临床资料、组织学形态特征及免疫组化标记。     

Degraded and undegraded carrageenans and experimental gastric and duodenal ulceration

The prevention of histamine-induced gastric and duodenal ulceration in the guinea-pig has been examined using a series of undegraded and degraded carrageenans. Undegraded carrageenans were active at lower doses than degraded carrageenans. The high viscosity of the undegraded carrageenans in solution prevented their use in larger doses. Degradation of carrageenan without serious loss of sulphate, gives a product which allows the dose to be increased to an extent that its effect more than offsets the slight loss in activity caused by the degradation. No single feature of carrageenan structure can be related to anti-ulcer activity although degradation, and hence reduction of molecular size, generally reduces activity. Sulphate contents over 30% have little apparent effect on activity; κ-carrageenans were not consistently different in anti-ulcer activity from Λ-carrageenans. This contrasts with the antipeptic activity of carrageenans where κ-carrageenans are less active than their Λ-counter-parts. As with antipeptic activity, the degree of anti-ulcer activity is probably determined by a combination of structural features which includes molecular size and polyanionic properties.     

Affective and Anxiety Disorders and Alcohol and Drug Dependence:

Depression and anxiety frequently coexist in patients with substance use disorders. This clinically-oriented article examiens the relationship between these conditions and emphasizes data showing that substances of abuse can cause signs and symptoms of both depression and anxiety. These substance-related syndromes appear to have a different course and prognosis than uncomplicated, independent anxiety and major depressive disorders, and clinicians should consider the role of alcohol and other drugs in all patients presenting with these complaints. The authors will also outline an approach for diagnosing and managing patients with the combination of a substance use and depressive or anxiety disorder.     

Alcohol and Substance Use and Abuse in Women and Children

No abstract available for this article.     

Glucosidase and fucosidase inhibitors and Advances in nucleosides and nucleotides.

The American Chemical Society Symposium "Glucosidase and fucosidase inhibitors" took place on 1 April 1998 and was organized by Professors Zbigniew J Witczak (UConn, School of Pharmacy, CT, USA), Kuniaki Tatsuta (Waseda University, Tokyo, Japan) and Waldemar Priebe, MD (Anderson Cancer Center, University of Texas, USA). Professor Witczak provided introductory remarks including the status of existing glucosidase inhibitors, and chaired the morning session, which consisted of six lectures. The symposium was well received, and was particularly attractive for those interested in networking, as attendance was about sixty. In addition, some participants and attendees presented posters on the subject during the regular poster session organized by the Division of Carbohydrate Chemistry.