Two new constituents from mangrove Bruguiera gymnorrhiza

A lignan and two aromatic compounds were isolated from the branches of the mangrove plant, Bruguiera gymnorrhiza. They were brugunin A (1), bruguierol D (2) and 2,3-dimethoxy-5-propylphenol (3). Among them, 1 and 2 were new compounds; 3 was isolated from a natural source for the first time. The structures of these compounds were determined by NMR spectroscopic studies as well as chemical evidence.     

Two new constituents from mangrove Bruguiera gymnorrhiza

A lignan and two aromatic compounds were isolated from the branches of the mangrove plant, Bruguiera gymnorrhiza. They were brugunin A (1), bruguierol D (2) and 2,3-dimethoxy-5-propylphenol (3). Among them, 1 and 2 were new compounds; 3 was isolated from a natural source for the first time. The structures of these compounds were determined by NMR spectroscopic studies as well as chemical evidence.     

New aromatic compounds from the marine mangrove Bruguiera gymnorrhiza

The phytochemical investigation of the stem of Bruguiera gymnorrhiza yielded five new aromatic compounds (1-5), of which the bruguierols A - C (1-3) represent a new structural skeleton in natural product chemistry. All structures have been determined by NMR spectroscopic studies. Among them, 3 showed moderate activity against Gram-positive and Gram-negative bacteria including mycobacteria and resistant strains (MICs 12.5 microg/mL).     

中国湛江木榄(Bruguiera gymnorrhiza)的化学成分研究

目的 对采自中国湛江红树植物木榄的乙酸乙酯萃取部分进行深入系统的化学成分研究.方法 采用硅胶柱色谱、凝胶柱色谱等手段,结合理化性质和波谱数据,对木榄的乙酸乙酯萃取部分的化合物进行分离鉴定.结果 从木榄的乙酸乙酯萃取部分中分离得到6个化合物.经现代波谱学分析(<'1>H-NMR,2D NMR,MS等),分别鉴定为:细胞松...     

Bruguiesulfurol, a new sulfur compound from Bruguiera gymnorrhiza

A new cyclic 4-hydroxy-dithiosulfonate, bruguiesulfurol (1), as well as two known 4-hydroxydithiolane 1-oxides, brugierol (2) and isobrugierol (3) were isolated from the flowers of Bruguiera gymnorrhiza. With stably-transfected HepG2 cells, the three isolates activated antioxidant response element (ARE) luciferase activation with (EC(50)) values of 56.7, 3.7 and 1.8 microM, respectively. Compounds 2 and 3 also inhibited phorbol ester-induced NF-kappaB (nuclear factor-kappaB) luciferase activity with IC (50) values of 85.0 and 14.5 microM, respectively. In addition, compound 2 inhibited enzyme cyclooxygenase-2 (COX-2) activity with an IC(50) value of 6.1 microM. The structures of these isolates were determined by spectral data, and that of compound 1 was confirmed by X-ray crystallographic analysis.     

环境因子对药用植物木榄遗传多态性及其分布的影响

目的探讨环境因子变化对木揽的DNA遗传多态性的影响。方法利用RP—PCR技术分析中国南部3省6个红树群落中未榄的DNA遗传多态性,并与6个群落环境因子数据对比。结果在扩增到的1277个DNA片段中,25．5％的DNA片断呈现多态性变化：聚类分析显示,来自于山口、高桥、东寨港以及三亚红树群落的木榄可襞为一支（群落问遗传相似系数大于0．90）。文昌和特呈岛木揽分别属于另外两支（与上述4个红树群落的遗传相似系数小于0．90,二者间的遗传相似系数小于0．85）;环境因子数据的聚类分析显示,这6个红树群落可分为4支,其中东寨港和文昌为一支,高桥和特呈岛为一支,山口以及三亚分别属于另外2支。结论地理位置的差异是导致木揽遗传水平出现多态性变化的重要原因之一。     

Isolation and expression analysis of a CBF transcriptional factor gene from the mangrove Bruguiera gymnorrhiza

Ecotoxicology - The present work isolated a CBF/DREB1 gene from mangrove Bruguiera gymnorrhiza (BgCBF1) and compared its expression levels in various tissues under normal condition and cold stress,...     

Two new 18-en-oleane derivatives from marine mangrove plant, Barringtonia racemosa

Two new triterpenoids, olean-18-en-3beta-O-E-coumaroyl ester (1) and olean-18-en-3beta-O-Z-coumaroyl ester (2), were isolated from the stem bark of marine mangrove plant Barringtonia racemosa, along with five known compounds, germanicol, germanicone, betulinic acid, lupeol, and taraxerol. Their structures were determined mainly by spectroscopic methods.     

Effects of pyrene on antioxidant systems and lipid peroxidation level in mangrove plants, Bruguiera gymnorrhiza

The effects of polycyclic aromatic hydrocarbon (PAH) (pyrene) on superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase, peroxidase, malondialdehyde (MDA) and proline were studied in leaves, stems and roots of Bruguiera gymnorrhiza. The results showed that the responses of enzymatic and non-enzymatic antioxidants varied significantly among the three tissues studied. The activities of antioxidant enzymes in PAH-treated stems and roots fluctuated in different stress levels compared to the controls, while the antioxidant enzymes such as SOD, APX in leaves increased when stressed by PAH with a significant positive relation between PAH and leaf SOD or APX activity. Low PAH treatments could also stimulate proline in leaves and stems. MDA content was obviously accumulated in stems and roots under PAH stress while decreased in leaves, indicating that the increased antioxidant enzymes in leaves may partly alleviate lipid peroxidation. For pollution monitoring purpose, SOD and APX in leaves may be potential biomarkers of PAH pollution in intertidal estuaries.     

(+)-Cyclopenol,a new naturally occurring 7-membered 2,5-dioxopiperazine alkaloid from the fungus Penicillium sclerotiorum endogenous with the Chinese mangrove Bruguiera gymnorrhiza

One new naturally occurring 7-membered 2,5-dioxopiperazine alkaloid named (+)-cyclopenol (1), along with nine known compounds including viridicatol (2), 3-(dimethylaminomethyl)-1-(1,1-dimethyl-2-propenyl)indole (3), anacine (4), aurantiomide C (5), viridicatin (6), 3-O-methylviridicatin (7), verrucosidin (8), ergosterol (9), and ergosterol peroxide (10), was isolated from the EtOAc extract of fungus Penicillium sclerotiorum, an endophytic fungal strain isolated from Chinese mangrove Bruguiera gymnorrhiza. The chemical structure of the new compound 1 was elucidated on the basis of detailed spectroscopic analysis. The absolute configuration of 1 was determined by single-crystal X-ray analysis with Cu Kα radiation (λ = 1.54178 Å). To our knowledge, (+)-cyclopenol (1) represents the first example of 7-membered 2,5-dioxopiperazine isolated from mangrove endophytic fungus.     

Three new diterpenoids from Aralia dumetorum

Three new diterpenoids, dumetoranes A (1) and B (2), melanocane B (3), together with four known ones including melanocane A (4), ent-15S,16-dihydroxypimar-8(14)-en-19-oic acid (5), ent-pimara-8(14),15-diene-19-oic acid (6), and ent-pimara-8(14),15-diene-19-ol (7) were obtained from the ethanol extract of the roots of Aralia dumetorum. Their structure elucidation was achieved by the methods of spectroscopic HRMS, IR, NMR, and by comparison with literature. The cytotoxicities of compounds 1–3 and 5 were assayed by in vitro MTT methods.     

Three new jatrophone-type diterpenoids from Euphorbia helioscopia

Three new jatrophone-type diterpenoids, euphoheliosnoids A-C, together with two known related compounds, were isolated from the Chinese medicinal plant Euphorbia helioscopia L. The structures including relative stereochemistry of the new compounds were elucidated by spectroscopic methods and by comparison with known related compounds.     

Three new diterpenoids from Coleus forskohlii Briq

Three new diterpenoids, forskolin G(2), forskolin H(3), forskolin I(4), were isolated from the whole plant of the Coleus forskohlii Briq., and their structures were elucidated as 1α,6β-diacetoxy-8,13-epoxylabd-14-en-11-one, 1α-hydroxy-6β,7β-diacetoxy-8,13-epoxylabd-14-en-11-one, and 1α,9α-dihydroxy-6β,7α-diacetoxy-8,13-epoxylabd-14-en-11-one on the basis of spectral data.     

Three new isopimarane diterpenoids from Excoecaria acerifolia

Three new isopimarane diterpenoids named excoecarins F–H (1–3) were isolated from the EtOAc extract of the Chinese ethnodrug Gua-jing-ban (Excoecaria acerifolia Didr.). Their structures were elucidated by the analysis of spectroscopic data including 1D, 2D NMR and HR-MS. The anti-HIV-1 bioactivity test of 1 and 2 showed weak activity.     

Three new diterpenoids from Coleus forskohlii Briq

Three new diterpenoids, forskolin G(2), forskolin H(3), forskolin I(4), were isolated from the whole plant of the Coleus forskohlii Briq., and their structures were elucidated as 1alpha,6beta-diacetoxy-8,13-epoxylabd-14-en-11-one, 1alpha-hydroxy-6beta,7beta-diacetoxy-8,13-epoxylabd-14-en-11-one, and 1alpha,9alpha-dihydroxy-6beta,7alpha-diacetoxy-8,13-epoxylabd-14-en-11-one on the basis of spectral data.     

Three new acyclic diterpenoids from Eupatorium lindleyanum DC

Three new acyclic diterpenoids were isolated from the whole plant of Eupatorium lindleyanum DC. The structures of the compounds were elucidated by means of (1)H and (13)C NMR spectroscopic analyses, including 2D NMR experiments.     

Three new diterpenoids from Mallotus apelta Muell.Arg

Three new diterpenoids, 10-hydroxy-cembrene-5-one, 6-hydroxy-cembrene-5,10-dione and 2alpha,4beta,15,16-tetrahydroxyl-dolabradane were isolated from the petroleum ether fraction of the alcoholic extract of Mallotus apelta Muell.Arg. Their structures were determined by spectral methods.     

Three new diterpenoids from the leaves of Thuja orientalis

Three new diterpenoids, 18-formyloxy-8 β-hydroxysandaracopimar-15-ene (1), 15(R)-N-butoxypinusolidic acid (2), and 15,16-dihydro-15,16-dimethoxylambertianic acid (3), along with twelve known compounds (4-15) were isolated from MeOH extracts of leaves of Thuja orientalis L. The structures of the three new compounds were elucidated on the basis of spectroscopic analyses, including extensive 2D-NMR data. The absolute stereochemistry of compound 2 was clarified by a CD spectroscopic study. The isolated compounds were evaluated for cytotoxicity against A549, SK-OV-3, SK-MEL-2, and HCT-15 cell lines in vitro using the sulforhodamin B (SRB) bioassay.     

New abietane diterpenoids from the mangrove Avicennia marina

Phytochemical investigations of the twigs of Avicennia marina yielded three new abietane diterpenoids 11-hydroxy-8,11,13-abietatriene 12- O-beta-xylopyranoside ( 1), and a pair of inseparable epimers 6 Halpha-11,12,16-trihydroxy-6,7-secoabieta-8,11,13-triene-6,7-dial 11,6-hemiacetal ( 2) and 6 Hbeta-11,12,16-trihydroxy-6,7-secoabieta-8,11,13-triene-6,7-dial 11,6-hemiacetal ( 3), as well as the new lignan (7' S,8' R)-4,4',9'-trihydroxy-3,3',5,5'-tetramethoxy-7,8-dehydro-9-al-2,7'-cyclolignan ( 5), together with 6,11,12,16-tetrahydroxy-5,8,11,13-abitetetraen-7-one ( 4), lyoniresinol ( 6), lyoniresinol 9'- O-beta- D-glucopyranoside ( 7), and diacetylmartynoside ( 8). Structure elucidation of the new compounds was accomplished by analysis of their spectroscopic data. Compounds 2 - 4 showed moderate cytotoxic and antimicrobial activities.     

红树林植物海莲内生真菌Penicillium sp.091402化学成分的研究

目的 对红树植物海莲内生真茵茵丝体提取物的乙酸乙酯萃取部分进行化学成分研究.方法 采用硅胶柱色谱等分离手段,利用理化教据和各种波谱分析方法,结合文献对照,对海莲内生真菌penicilium sp.091402菌丝体中化学成分进行分离鉴定.结果与结论 从海莲内生真菌penicilium sp.091402菌丝体乙酸乙酯萃取部分分离得到6个化合物,分别鉴定为:(3β,22E)-麦角甾-5,7,22-三烯-3-醇(1),(313,5α,8α,22E)-5,8-过氧麦角甾-6,22-二烯-3-醇(2),(3β,5α,6α,7α,22E)-5,6-环氧麦角甾-8(14),22-=-烯-3,7-二醇(3),(3β,5α,6β,22E)-麦角甾-7,22-二烯-3,5,6-三醇(4),soyasapogenol B(5)和citrinin H2(6).