增殖细胞核抗原及Ki-67在口腔白斑和鳞癌中的表达及其相关性

目的：比较研究增殖细胞核抗原（PCNA）和Ki-67在口腔癌前病变中的表达，方法：选取人正常口腔粘膜（NOM）（8例），口腔白斑（LK）（31例）及口腔鳞状细胞癌（OSCC）（22例）标本，同时和LSAB法染色PCNA和Ki-67表达，分析增殖活性，用SPSS9．0统计软件分析各组间各指标的差异，PCNA指数和Ki-67指数的相关性。结果：在NOM上皮及单纯增生上皮内，PCNA阳性细胞散在分布于上皮基底层细胞，统计学分析二者无差异。在异常增生上皮各组中，随着增生程度的病理分级的增加，PCNA阳性细胞数增加，分布从其底层向表层增加。且不同增生程度各组间统计学上有显著性差异。在高分化鳞癌中，主要分布在癌巢周围及肿瘤浸润前沿区域。Ki-67与PCNA分布特征类似，但其指数普遍低于PCNA指数。Pearson相关系数r=0．79,二者呈线性正相关。结论：PCNA及Ki-67与口腔粘膜增殖异常有关，其增殖指数与增生程度平行，且二者本身呈线性相关。     

CD147和Ki-67在口腔黏膜白斑和鳞癌组织中的表达及意义

目的：观察CD147和ki-67在口腔正常黏膜、白斑和鳞癌组织中表达的变化,阐明CD147在口腔癌发病机制中的作用。方法：采用免疫组织化学法检测10例正常口腔黏膜、20例白斑伴上皮异常增生上皮和40例鳞癌组织中CD147及Ki-67的表达变化。结果：CD147在正常黏膜阴性表达,白斑和鳞癌组上皮均显著表达CD147;正常组Ki-67表达主要位于上皮棘层,鳞癌组织中Ki-67阳性细胞分布广泛,有大部分侵入固有层中。结论：口腔黏膜发生癌前病变时,即出现CD147表达阳性,随着Ki-67阳性细胞数的增多,癌变细胞增殖不断加强,两者共同作用促进鳞癌的发展。     

PCNA/AgNOR and Ki-67/ AgNOR double staining in oral squamous cell carcinoma

This study was performed on oral squamous cell carcinomas (OSCC) in order to investigate the relation between the number of interphase silver-stained nucleolar organizer regions (AgNORs) and the immunolabeling of proliferation-associated markers, using antibodies to Ki-67 and proliferating cell nuclear antigen (PCNA). Fifteen consecutive cases of oral squamous cell carcinoma were used and a double staining technique was performed in order to quantify the number of NORs in PCNA-positive and -negative cells as well as in Ki-67-positive and -negative cells. Our results showed a higher mean number of AgNORs in PCNA- and Ki-67-positive cells than in PCNA- and Ki-67-negative cells. We concluded that there is an association between cell proliferation and AgNOR score in OSCC.     

口腔鳞癌组织中Ki-67和p53蛋白的表达

目的 探讨口腔鳞癌组织中Ki-67和p53蛋白的表达及其与临床病理特征的关系。方法采用免疫组织化学S-P法对10例正常口腔黏膜组织、16例口腔白斑（OLK）组织、48例口腔鳞癌（OSCC）组织中的Ki-67和p53蛋白表达进行检测,结合患者临床病理资料进行分析,使用SPSS17.0 软件包对数据进行统计学处理。结果Ki-67蛋白在正常口腔黏膜组织、口腔白斑和口腔鳞癌组织中的阳性表达率分别为30.0%、56.3%和79.2%;p53的阳性表达率分别为0.0%、43.8%和70.8%,Ki-67和p53在正常黏膜组与口腔白斑和口腔鳞癌组差异均具有显著性（P<0.05）;Ki67蛋白在口腔鳞癌组织中的表达与肿瘤的临床分期、分化程度、有无淋巴结转移有关（P<0.05）,p53蛋白的表达与肿瘤的分化程度有关(P<0.05);Ki-67和p53蛋白在口腔鳞癌组织中的表达呈正相关（P<0.05）。结论Ki-67和p53蛋白在口腔鳞癌组织中高表达,可能在口腔鳞癌的发生、发展过程中起着重要作用。     

Ki-67、 PI3K、 Beclin1在口腔鳞癌组织中的表达及意义

目的：检测Ki-67、磷酸肌醇-3-激酶（PI3K）、Beclin1在口腔鳞癌组织中的表达及意义。方法：选取2017年1月—2018年12月南京市口腔医院收治的口腔鳞癌患者30例,取所有手术切除癌组织及癌旁组织标本,进行免疫组织化学染色处理,并检测Ki-67、PI3K、Beclin1的表达,采用Pearson分析TMSG-1、Ki-67、Pgp1之间的相关性。采用SPSS 20.0软件包对数据进行统计学分析。结果：口腔鳞癌癌组织中Ki-67、PI3K阳性表达率显著高于癌旁组织,Beclin1阳性表达率显著低于癌旁组织（P<0.05）。Ki-67、PI3K、Beclin1在高分化口腔鳞癌中的阳性表达率显著高于中分化、低分化口腔鳞癌（P<0.05）。Ki-67、PI3K在有淋巴结转移的口腔鳞癌中的阳性表达率显著高于无淋巴结转移的口腔鳞癌,Beclin1在有淋巴结转移的口腔鳞癌中的阳性表达率显著低于无淋巴结转移的口腔鳞癌（P<0.05）。Ki-67、PI3K、Beclin1在Ⅰ+Ⅱ期、Ⅲ+Ⅳ期口腔鳞癌中的阳性表达率无统计学差异（P>0.05）。Ki-67与PI3K的表达呈正相关（r=0.391,P=0.032）,Ki-67与Beclin1的表达呈负相关（r=-0.525,P=0.02）,Beclin1与PI3K的表达呈负相关（r=-0.367,P=0.045）。结论：Ki-67、PI3K、Beclin1的表达具有相关性,与患者有无淋巴结转移、病理分期有关,可能参与口腔鳞癌发生与发展。     

Analysis of the Ki-67 antigen at the invasive tumour front of human oral squamous cell carcinoma

BACKGROUND: It is hypothesised that cell proliferation, as measured by the Ki-67 labelling index (LI) at the invasive tumour front (ITF) was directly related to the histological grade in human oral squamous cell carcinomas (SCCs). METHODS: Tissues from 42 human oral SCCs were collected and stained with an antibody directed against the Ki-67 antigen using an advanced polymer staining system. Quantitation of the immunopositive cells was performed on two parallel sections at the invasive tumour front (ITF), using an image analyser. The Ki-67 LI was expressed as the number of positive nuclei/mm2 of epithelium. The control tissue used was normal epithelium at the excision margin. RESULTS: The mean Ki-67 LI for oral SCCs at the ITF was significantly greater than that for the excision margin tissue (P < 0.0001). There was a positive association between increasing Ki-67 LI and increasing Broders' grade (P < 0.05), with a well-differentiated tumour having the lowest mean Ki-67 LI (1549 +/- 806) and a poorly differentiated tumour having the highest value (2232 +/- 771). A similar trend was observed between the mean Ki-67 LI and Bryne's multifactorial grading system. CONCLUSIONS: It was concluded from this study that cell proliferation (as measured by the Ki-67 antigen) at the ITF had a strong positive relationship with histological grading in human oral SCC.     

Maspin expression in oral squamous cell carcinoma

Maspin (mammary serine protease inhibitor) is a member of the serpin superfamily of protease inhibitors and it has a role as a tumor suppressor. Maspin has been reported to be important in processes relevant to tumor growth and metastasis such as cell invasion, angiogenesis, and apoptosis. A high expression of maspin was correlated with better rates of survival and absence of nodal metastases in head and neck squamous cell carcinoma. In contrast, some studies have shown that maspin overexpression is correlated with a poor prognosis in pancreatic and ovarian cancers and in lung adenocarcinoma. The aim of this study was an immunohistochemical evaluation of the maspin expression in oral squamous cell carcinoma and thus 89 patients were evaluated. Maspin expression in oral squamous cell carcinoma was significantly associated with the tumor differentiation grade (chi test: P = 0.0318) and the lymph node status (chi test: P < 0.005), but not with the tumor stage (chi test: P = 0.666). Metastatic involvement of lymph nodes was observed more frequently in maspin-negative cases than in tumors with more than 5% of positive cells (P = 0.0024). The present results confirm that maspin expression predicts a better prognosis in oral squamous cell carcinoma and that maspin probably plays a role in tumor progression.     

透明质酸在口腔鳞状细胞癌组织中的表达

目的 本研究旨在探讨透明质酸在不同分化口腔鳞状细胞癌中的表达及意义.方法 应用免疫组织化学法检测37例不同分化程度口腔鳞状细胞癌组织中透明质酸的表达.结果 按阴性(-)、弱阳性( )、阳性( )、强阳性( )表示,并进行统计学分析.结果 透明质酸主要表达于肿瘤间质和细胞外基质,细胞膜和胞浆中染色相对较少.37例口腔鳞状细胞癌组织中,低分化鳞癌透明质酸的表达明显高于高分化鳞癌(P＜0.05),表达随肿瘤分化程度降低而增强.结论 透明质酸的表达与口腔鳞状细胞癌的分化程度有关,分化越低,其表达越强.透明质酸的高表达可能有利于病变的侵袭和转移.     

P27kipl��Ki-67�ڿڵ���״ϸ�����еı�Ｐ����

目的    探讨P27kipl和Ki-67在口底鳞状细胞癌中的表达水平及其与肿瘤分化程度、淋巴结转移、预后的关系。方法    收集枣庄矿业集团中心医院病理科1998年1月至2004年12月的口底鳞状细胞癌手术根治标本蜡块及其相应的癌旁正常黏膜组织（距肿物1.5 cm）蜡块各45例，应用免疫组织化学染色S-P方法进行P27kipl、Ki-67基因蛋白的检测。结果    癌旁正常黏膜中，P27kipl高表达率为75.56%、Ki-67阳性表达率为8.89%，口底鳞状细胞癌中P27kipl高表达率为22.22%、Ki-67阳性表达率为35.56%，经比较两组间差异均有统计学意义（均P＜0.01）。口底鳞状细胞癌分化较好者，P27kipl高表达率（26.92%）高于分化较差者（15.79%），但差异无统计学意义（P＞0.05）；Ki-67阳性表达率在分化较好者（23.08%）与较差者（52.63%）间差异有统计学意义（P＜0.05）。P27kipl高表达率、Ki-67阳性表达率在有、无淋巴结转移中比较，差异均有统计学意义（均P＜0.05）。P27kipl高表达率、Ki-67阳性表达率在术后存活期中比较，差异亦均有统计学意义（均P＜0.05）。结论    P27kipl、Ki-67在口底鳞状细胞癌中起重要的调控作用，并是判断其预后的重要参考指标。     

Cyclin D1 and Ki-67 expression correlates to tumor staging in tongue squamous cell carcinoma

Background

The immunohistochemical expression of Cyclin D1 and Ki-67 were analyzed in tongue squamous cell carcinomas (SCC), relating them to the clinical and morphological exhibition of these tumors.

Material and Methods

Twenty-nine patients fulfilled the inclusion criteria; clinical data included gender, age, ethnicity and use of licit drugs such as alcohol and tobacco. The TNM staging and histopathological differentiation grading was assessed for each case. In addition, T1 patients were gathered with T2 patients; and T3 patients were gathered with T4 patients to assemble two distinct groups: (T1/T2) and (T3/T4).

Results

The mean follow-up time was 24 months and 30% of the patients died as a consequence of the disease, while 23.3% lived with the disease and 46.7% lived lesion-free. T1 and T2 tumors showed statistically lesser Ki-67 and Cyclin D1 staining when compared to T3 and T4 tumors.

Conclusions

Ki-67 and Cyclin D1 pose as auxiliary tools when determining the progression of tongue SCC at the time of diagnosis. Key words:Carcinoma, squamous cell, cyclin D, immunohistochemistry, Ki-67 antigen, prognosis.     

CD44在口腔鳞癌中的表达

目的初步探讨CD44分子在口腔鳞癌中的表达情况。方法以免疫组织化学方法对22例口腔不同部位的鳞癌及其周边扩大切除的组织进行了观察研究,并和癌组织的病理分级进行对比分析。结果 CD44表达在正常口腔粘膜表皮的基底细胞和部分棘层细胞的细胞膜上,同时在淋巴细胞上强表达。在鳞癌上皮组织中CD44的表达模式发生紊乱,表达水平与细胞分化程度有关,病理分化度较差的癌组织中CD44的表达逐渐减弱。结论 CD44可以作为评价口腔鳞癌恶性度的一个指标。     

基底细胞痣综合征的牙源性角化囊肿中Ki-67表达的研究

目的：探讨基底细胞痣综合征的牙源性角化囊肿细胞增殖活性与临床生物学行为的关系。方法：利用Ki-67单克隆抗体，免疫组化方法（LSAB法）检测基底细胞痣综合征的牙源性角化囊肿和非综合征的牙源性角化囊肿中Ki-67表达情况。结果：Ki-67在基底细胞痣综合征的牙源性角化囊肿衬里上皮中的表达主要位于基底上层，且明显高地单发和复发牙源性角化囊肿，结论：基底细胞痣综合征的牙源性角化囊肿较非综合征的角化囊肿具有更高的细胞增殖殖活性，与其较高的复发潜能有关。     

EGFR and Ki‐67 expression in oral squamous cell carcinoma using tissue microarray technology

J Oral Pathol Med (2010) 39 : 571–578 Objective: Our aim was to validate the use of tissue microarrays (TMA) in oral squamous cell carcinomas (OSCC) to analyse epidermal growth factor receptor (EGFR) and Ki‐67 expression. We also analysed the relationship that the expression of these markers may have with clinical, pathological and survival variables. Patients and methods: The study sample comprised 39 unselected patients diagnosed and treated for OSCC. We analysed Ki‐67 and EGFR expression by immunohistochemistry on formalin‐fixed, paraffin‐embedded surgical specimens. Whole sections (WS) were compared with double 1.5 mm core‐tissue microarrays. Results: High EGFR expression was observed both on TMA (in 98% of the cases) and WS (in 100% of the cases) with substantial agreement kappa value (0.720). EGFR expression was not significantly associated with clinical, pathological and survival variables on TMA and WS. Ki‐67 analysis showed a Spearman correlation of 0.741 with a Ki‐67 mean labelling index of 45% in TMA and 56.8% in WS. We found a significant relationship between gender and Ki‐67 labelling index on WS (P = 0.022) and TMA (P = 0.002). Clinical stage was the only parameter in multivariate analysis that had a significant predictive value. Conclusion: We demonstrate that dual 1.5 mm core TMA is a valid, rapid, economical and tissue‐saving way to study OSCC biopsies and that it presents strong correlation with the WS. EGFR overexpression in OSCC suggests that these tumours may be a candidate for therapy investigation directed to EGFR.     

shRNA干扰Rce1基因表达对口腔鳞癌细胞放射敏感性的影响

目的:观察shRNA干扰Ras转化酶1（Rce1）基因表达对口腔鳞癌（OSCC）细胞放射敏感性的影响。方法:取对数期CAL27细胞,随机分为Control组、shRNA-NC组、shRNA-Rce1组。Control组不处理,shRNA-NC组、shRNA-Rce1组分别采用脂质体转染法转染shRNA-NC、shRNA-Rce1表达载体。采用二甲基噻唑（MTT）法检测不同放射剂量（2、4、6、8、10 Gy）射线照射对CAL27细胞增殖抑制率的影响,计算放射对细胞的半数抑制剂量。利用AnnexinV-FITC/PI双染法检测细胞凋亡率,Western免疫印迹法检测细胞B细胞淋巴瘤2（Bcl-2）、存活蛋白（Survivin）、半胱天冬氨酸蛋白酶3（Caspase-3）蛋白表达量。采用SPSS 19.0软件包对数据进行统计学分析。结果:与Control组、shRNA-NC组相比,不同放射剂量对shRNA-Rce1组CAL27细胞的增殖抑制率显著升高,半数抑制剂量显著降低（P<0.05）;与Control组、shRNA-NC组相比,shRNA-Rce1组凋亡率显著升高（P<0.05）;与Control组、shRNA-NC组相比,shRNA-Rce1组Bcl-2、Survivin蛋白表达量显著降低,Caspase-3蛋白表达量显著升高（P<0.05）。结论:shRNA干扰Rce1基因表达可增强OSCC细胞放射敏感性,降低半数抑制剂量,促进细胞凋亡,并减少凋亡抑制蛋白Bcl-2、Survivin表达,增加凋亡蛋白Caspase-3表达。     

口腔疣状癌与口腔鳞状细胞癌差异基因表达的对比分析

目的研究口腔疣状癌与口腔鳞癌组织基因的差异表达，探讨口腔疣状癌（oral Verrucous carcinoma，OVC）与口腔鳞癌（oral squamous cell carcinoma，OSCC）的基因学基础。方法应用cDNA芯片技术对4例OVC和4例OSCC组织mRNA检测，通过芯片杂交、生物信息学处理，找出两者间差异表达基因。结果BioStarH-40芯片发现差异表达基因593条，差异表达基因占15．2％，其中表达增强283条（显著增强59条），表达降低310条（显著降低98条）。结论OVC与OSCC基因表达比较，差异有统计学意义，这些差异可能在各自不同的生物学行为中起重要作用。     

Midkine expression in oral squamous cell carcinoma and leukoplakia

J Oral Pathol Med (2012) 41 : 21–26 Background: Midkine (MK), a 13‐kDa heparin‐binding growth factor, is overexpressed in various human cancers. However, its role in the development and progression of oral cavity squamous cell carcinoma (OCSCC) is still unclear. Thus, the aim of this study was to evaluate the expression of MK in samples of OCSCC, leukoplakia, and healthy oral mucosa (control). Methods: Surgically excised specimens from patients with primary OCSCC (n = 28) were immunostained for MK, Ki‐67, PCNA, p53, bcl‐2, Bax, and CD31. Besides this, MK expression was also investigated in leukoplakia and normal oral mucosa. The relationship of MK⁺ cells with clinical parameters (tumor location, tumor size, lymph node metastasis, and survival) and microscopic parameters (WHO histological grading, intensity of inflammation, proliferation index, apoptosis, and angiogenesis) was also evaluated. Results: The results showed that MK expression was increased in OCSCC in relation to leukoplakia and normal mucosa. Furthermore, MK expression was increased in late‐stage tumors (T3/T4) compared with early‐stage lesions (T1/T2). MK‐positive lesions also showed increased expression of the anti‐apoptotic protein bcl‐2. Conclusion: OCSCC, particularly late‐stage tumors, exhibits increased MK expression, which may be involved in tumor progression via upregulation of anti‐apoptotic genes, as shown by the augmented bcl‐2 positivity in MK‐positive tumors.