游离股前外侧穿支皮瓣修复头部电烧伤创面

目的：探讨游离股前外侧穿支皮瓣在头部电烧伤创面修复中的应用。方法回顾性分析应用游离股前外侧穿支皮瓣修复4例电烧伤患者头部电烧伤创面的治疗方法及其临床效果。结果4例股前外侧穿支皮瓣顺利成活,头部外形恢复良好。结论应用游离股前外侧穿支皮瓣修复头部电烧伤创面,供瓣区损伤小,无需损伤主干血管,组织块合适,无需二期修薄,缩短治疗周期有重要的意义。     

股前外侧皮瓣在治疗胫骨骨髓炎中的应用

目的 探讨游离股前外侧皮瓣在胫骨骨髓炎伴有皮肤软组织缺损中的疗效.方法 应用旋股外侧动脉横支或降支为蒂切取皮瓣另一支桥接胫前或胫后动脉、闭合创面、改善足部血运11例.结果 临床应用11例,3例60岁左右并伴有糖尿病患者,需二次手术小量自身骨移植,其余患者创面一期愈合,骨折愈合良好.结论 股前外侧皮瓣血管解剖位置恒定,血管口径较粗,利用横支或降支蒂另一支可桥接血管,改善足部血运,对治疗胫骨骨髓炎有较好的疗效.     

游离股前外侧皮瓣修复四肢皮肤软组织缺损12例临床疗效分析

目的:探讨游离股前外侧皮瓣修复四肢皮肤软组织缺损的临床疗效。方法:2008年1月～2012年6月,采用吻合血管的游离股前外侧皮瓣修复四肢皮肤软组织缺损12例,皮瓣切取面积8cm×12cm～24cm×14cm。结果:12例皮瓣均成活,术后随访3～18个月,所有患者创面愈合良好,功能恢复满意。结论:游离股前外侧皮瓣存活率高,血运丰富,部位隐蔽,是修复四肢皮肤软组织缺损的理想皮瓣。     

应用游离股前外侧皮瓣修复头面部软组织缺损15例报告

目的探讨股前外侧游离皮瓣修复头面部软组织缺损的临床效果。方法额部及面颊部皮肤软组织缺损患者15例,应用吻合血管的股前外侧皮瓣移植修复,结果15例全部成活,1例发生血管危象,经处理后皮瓣成活,1例远端表皮起水泡,经换药后成活。结论游离股前外侧皮瓣能提供充足的组织量且存活力高,供区隐蔽,不牺牲重要血管,适用于头面部软组织缺损的修复效果好。     

股前外侧皮瓣的临床应用

目的 探讨利用股前外侧皮瓣修复皮肤软组织缺损的临床疗效.方法 对2012年3月-2016年3月苏州大学附属第一医院收治的32例皮肤软组织缺损患者采用股前外侧皮瓣游离移植修复.皮瓣切取范围为12 cm×6 cm~22 cm×13 cm.结果 该组32例皮瓣全部成活,创面均Ⅰ期愈合.术后无1例发生血管危象.随访6~24个月,平均16个月.所有皮瓣外形及功能均满意.结论 临床应用股前外侧皮瓣游离移植修复皮肤软组织缺损是一种较为理想的方法.     

股前外侧皮瓣修复四肢组织缺损的临床应用

目的报道应用股前外侧皮瓣修复四肢皮肤软组织缺损的治疗效果。方法应用吻合血管的股前外侧皮瓣修复前臂、小腿、足跟部及足部皮肤软组织缺损18例。皮瓣面积最小10cmx7cm,最大26cmx15cm。结果术后移植皮瓣全部成活,其中2例游离皮瓣术后发生血管危象,经探查处理后成活。结论应用股前外侧皮瓣是修复四肢皮肤软织缺损较为理想的方法,临床效果良好。     

游离股前外侧皮瓣修复手足创面

目的探讨游离股前外侧皮瓣移植修复四肢远端创面的临床疗效。方法收集2008年5月-2012年3月我院选用游离股前外侧皮瓣移植修复手足创面的病例45例,术后38例随访6个月-3年不等。结果股前外侧皮瓣移植45例,完全成活41例;1例改行其他术式（腓肠神经营养岛状皮瓣）,其中3例患者边缘有部分坏死,在经皮片移植后完全愈合,皮瓣携带股外侧皮神经并将其与受区皮神经吻合15例。结论游离股前外侧皮瓣是修复手足创面的良好方法。     

股前外侧皮瓣游离移植修复足踝部组织缺损

目的观察游离股前外侧皮瓣移植修复足踝部组织缺损的临床效果.方法应用游离股前外侧皮瓣移植治疗足踝部组织缺损27例,缺损面积12cm×7cm-25cm ×15cm.术前、术中受区创面彻底清创,切取股前外侧皮瓣修复创面,术后常规三抗治疗,及时处理血管危象.结果27例均获随访,时间6-36个月.出现血管危象2例,经及时处理后皮瓣恢复血液循环1例,1例探查失败,皮瓣修薄成游离全厚皮移植全部成活.结论游离股前外侧皮瓣供区隐蔽,成活率高,皮瓣质地优良,特别适合足底、足跟及足踝部皮肤软组织缺损以及肌腱、骨外露患者,是修复足踝部皮肤软组织缺损的理想皮瓣.     

吻合血管的股前外侧皮瓣移植修复足踝部皮肤软组织缺损(附4例报告)

目的探讨股前外侧皮瓣修复足踝部皮肤软组织缺损的临床应用价值。方法采用吻合血管的股前外侧皮瓣游离移植修复足踝部皮肤软组织缺损。切取皮瓣最大18cm×10cm,血管蒂长10cm,动脉口径约2mm,静脉口径约3mm。结果4例皮瓣完全成活。随访6～18个月,皮瓣厚薄适中,无臃肿,足踝部活动正常。结论股前外侧皮瓣是修复足踝部皮肤软组织大块缺损的有效方法之一。     

股前外侧皮瓣修复足部皮肤组织缺损

目的:报道应用股前外侧皮瓣修复足部皮肤软组织缺损的临床效果和体会。方法:应用吻合血管的股前外侧皮瓣游离移植修复10例足部皮肤软组织缺损,切取皮瓣面积最小为14cm×9cm,最大为18cm×15cm结果10例皮瓣全部成活.术后随访6~24个月,皮瓣没有出现溃疡,创面全部愈合。结论:股前外侧皮瓣是修复足部皮肤软组织缺损的有效方法之一。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.