念珠菌对激光的敏感性试验

目的：研究激光对念珠菌的杀菌效果。方法：应用457．9nm、632．8nm激光及CO2激光辐照经不同方式处理的念珠菌。结果：457．9nm及632．8nm激光对念珠菌均有一定的杀伤效应，457．9nm70mW激光作用40min致死率最高为62．9％。在光斑直径5mm输出功率3W条件下，CO2激光对念珠菌及蜗牛酶消化后的念珠菌杀菌的最小时间为20s及10s。结论：CO2激光杀菌彻底，效果显著好于457．9nm及632．8nm激光。     

吉西他滨对膀胱癌细胞敏感性的实验研究

目的:探讨吉西他滨体外对人膀胱癌T24细胞株,以及对不同个体膀胱癌细胞生长抑制的影响,明确吉西他滨对膀胱癌细胞有无明显抑制效应,是否适用于浅表性膀胱癌的术后灌注化疗.方法:体外培养膀胱移行细胞癌T24细胞,用生物荧光肿瘤体外药敏检测技术(ATP-TCA)检测不同浓度吉西他滨对肿瘤胞生长抑制的影响,光学显微镜观察不同时间段肿瘤细胞形态学变化.收集我院经尿道膀胱肿瘤切除术的40例浅表性膀胱癌患者的新鲜肿瘤标本,应用ATP-TCA技术检测肿瘤细胞对的吉西他滨的敏感率.结果:T24膀胱癌细胞株对吉西他滨表现为耐药;吉西他滨作用T24膀胱癌细胞株一定时间后,与对照组相比未见明显细胞凋亡或坏死;ATP-TCA法检测40例不同个体膀胱癌细胞对吉西他滨耐药率为90％.结论:吉西他滨体外对膀胱癌T24细胞株无生长抑制作用,对40例不同个体膀胱癌细胞耐药率为90％,初步说明该药不适合浅表性膀胱癌的术后灌注化疗.     

体外支原体药物敏感性的实验研究

目的：比较常用抗生素对临床标本中泌尿生殖道支原体的抗菌作用。方法：采用液体培养法，对临床标本中168株解脲支原体（Uu)和51株人型支原体（Mh)对10种常用抗菌药物的敏感性进行测定。结果：168株Uu对强力霉素，美满霉素，环丙沙星，氧氟沙星，罗红霉素，白霉素，交水霉素，螺旋霉素，阿齐霉素，氯霉素的药敏率分别为36．3％，45．2％，85．7％，70．2％，84.5%,20.2%,20.2%,4.8%,54.8%,22.6%，51株Mh对强力霉素，美满霉素，环丙沙星，氧氟沙星，罗红霉素，白霉素，交沙霉素，螺旋霉素，阿齐霉素，氯霉素的药敏率分别为23.5%,43.1%,90.2%,64.7%,78.4%,15.7%,17.6%,9.8%,74.5%,43.1%，结论：加强以支原体药敏测定，对指导临床治疗有重要意义。     

Increased Sensitivity to Heparin Following Acute Myocardial Infarction

In vivo increased sensitivity to heparin has been demonstrated in patients following an acute myocardial infarction. An intravenous injection of 10,000 units of heparin was given to each of 18 patients with recent myocardial infarction in order to compare them with 17 patients who were not suffering from any acute illness. The changes in whole blood clotting time, recalcified plasma clotting time and prothrombin time were greater and more prolonged in the patients with recent myocardial infarction. Of the three tests, the one-stage prothrombin time provided the simplest and the most precise measurement of heparin sensitivity. The reason for this was not clear: it is possible that it is related to shock and congestive heart failure which were complications of the clinical course following myocardial infarction.     

MTT法检测神经胶质瘤对化疗药物敏感性的研究

采用MTT法检测36例人神经胶质瘤新鲜标本对临床12种常用化疗药物的敏感性.作者分别对肿瘤标本的单细胞分离、接种细胞数、药物剂量及作用时间等影响MT T检测的多种因素进行系统研究,以每孔5×104个细胞接种、1×PPC(血浆峰值浓度)作用72 小时为适宜的试验条件,在此基础上的检测结果显示:神经胶质瘤对鬼臼噻吩苷、顺铂和丝裂霉素较为敏感,而不同个体对同一药物及不同药物对同一个体的敏感性差异较大.