共查询到20条相似文献,搜索用时 15 毫秒
1.
目的通过测定TBSA30%Ⅲ度烧伤大鼠早期手术后肝血流量的变化,了解早期手术对肝脏血液灌流的影响。方法Wistar雄性大鼠34只,分为4组:烧伤组(A组),输液组(B组),早期手术组(C组),正常对照组(D组)。输液组经股静脉插管输入乳酸林格氏液;早期手术组于立即补液的同时行切痂同种异体植皮术。各组于伤后0,0.5,1,2,3,6,12和24小时,应用氢清除法测定肝血流量。结果A组与B组伤后均出现肝血流量显著下降,B组直至伤后24小时肝血流量才恢复正常,而C组伤后肝血流量保持相对稳定,与正常对照组比较无显著意义。结论严重烧伤后立即液体复苏尚不足以维持有效的肝脏血流灌流,烧伤后早期手术尽早地去除焦痂组织,对改善肝血流量具有重要作用 相似文献
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目的通过测定 TBSA30%Ⅲ度烧伤大鼠早期手术后肝血流量的变化,了解早期手术对肝脏血液灌流的影响。方法 Wistar 雄性大鼠34只,分为4组:烧伤组(A 组),输液组(B 组),早期手术组(C 组),正常对照组(D 组)。输液组经股静脉插管输入乳酸林格氏液;早期手术组于立即补液的同时行切痂同种异体植皮术。各组于伤后0,0.5,1,2,3,6,12和24小时,应用氢清除法测定肝血流量。结果 A 组与 B 组伤后均出现肝血流量显著下降,B 组直至伤后24小时肝血流量才恢复正常,而 C 组伤后肝血流量保持相对稳定,与正常对照组比较无显著意义。结论严重烧伤后立即液体复苏尚不足以维持有效的肝脏血流灌流,烧伤后早期手术尽早地去除焦痂组织,对改善肝血流量具有重要作用。 相似文献
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早期肠道喂养对烧伤大鼠肠道一氧化氮合酶的影响 总被引:14,自引:0,他引:14
OBJECTIVE: Our previous studies have proved that early enteral feeding could improve intestine blood flow after burn injury. But the mechanism was far from being clarified. This study was attempted to explore the effects of early enteral feeding on nitric oxide synthase (NOS) activity in burned rat small intestine and the relationship between the intestine mucosa blood flow (IMBF) and the activity of NOS. METHODS: The rats were randomly divided into three groups: burned control (B), burned and early enteral feeding (EF), and normal control (C). The activity of NOS including constitute NOS(cNOS) and inducible NOS(iNOS), and the IMBF were determined at postburn 0, 3, 6, 12, 24, 48 hours. RESULTS: It was found that cNOS activity and IMBF were decreased markedly postburn, and there was positive correlation between cNOS and IMBF (r = 0.97, P < 0.01). But the activity of iNOS, total NOS were increased significantly postburn, they had no correlation to the IMBF. In EF group cNOS activity and the IMBF were significantly higher, the iNOS was obviously lower than that of B group and there was no significant difference of total activity of NOS between two groups. CONCLUSION: Our results suggested that NOS which is catalyzed from cNOS may play main role in adjusting IMBF. By using early enteral feeding the activity of cNOS is increased and the ischemic state in small intestine is improved after burn injury. 相似文献
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早期肠道喂养对烧伤大鼠肠道一氧化氮合酶的影响 总被引:1,自引:0,他引:1
目的阐明烧伤大鼠经早期肠道喂养后肠组织中一氧化氮合酶(NOS)的变化规律及其与肠粘膜血流量(IMBF)的关系。方法采用30%TBSAⅢ度烧伤大鼠模型,分为正常对照(C)组,单纯烧伤(B)组和早期喂养(EF)组,分别检测伤前及伤后0,3,6,12,24,48小时肠组织中NOS活性,包括原生型NOS(cNOS)和诱导型NOS(iNOS),并测定肠粘膜血流量。结果烧伤后各组cNOS和IMBF呈下降趋势,二者呈显著正相关(r=0.97,P<0.01),而NOS总活性和iNOS活性都呈上升趋势,IMBF与iN-OS和总NOS相关不显著。烧伤后EF组cNOS和IMBF明显高于B组,iNOS则低于B组,NOS总活力两组无显著差异。结论①两型NOS中cNOS与IMBF的关系更加密切。②早期肠道喂养改善烧伤后肠道缺血状况,可能与食物对肠壁神经的刺激从而提高cNOS活性有关。 相似文献
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早期肠道喂养对烧伤大鼠肠道一氧化氮合酶的影响 总被引:1,自引:0,他引:1
目的阐明烧伤大鼠经早期肠道喂养后肠组织中一氧化氮合酶(NOS)的变化规律及其与肠粘膜血流量(IMBF)的关系。方法采用30%TBSAⅢ度烧伤大鼠模型,分为正常对照(C)组,单纯烧伤(B)组和早期喂养(EF)组,分别检测伤前及伤后0,3,6,12,24,48小时肠组织中 NOS 活性,包括原生型 NOS(cNOS)和诱导型 NOS(iNOS),并测定肠粘膜血流量。结果烧伤后各组 cNOS 和 IMBF 呈下降趋势,二者呈显著正相关(r=0.97,P<0.01),而 NOS 总活性和 iNOS 活性都呈上升趋势,IMBF 与 iN-OS 和总 NOS 相关不显著。烧伤后 EF 组 cNOS 和 IMBF 明显高于 B 组,iNOS 则低于 B 组,NOS 总活力两组无显著差异。结论①两型 NOS 中 cNOS 与 IMBF 的关系更加密切。②早期肠道喂养改善烧伤后肠道缺血状况,可能与食物对肠壁神经的刺激从而提高 cNOS 活性有关。 相似文献
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烧伤早期营养对大鼠降钙素基因相关肽影响的实验研究 总被引:3,自引:0,他引:3
目的观察烧伤早期不同营养途径对肠道的复苏效应,及其与降钙素基因相关肽(cal-citoningene-relatedpeptide,CGRP)的关系方法30%TBSAⅢ度烧伤Wistar大鼠,随机分为灌喂组(EF组,30只)及早期静脉营养组(EPN组,30只).此外,6只大鼠不烧伤,作对照组(C组).伤后6、12、24、48、72h观察大鼠一般情况、肠黏膜血流量、血浆CGRP免疫活性物质浓度、CGRP染色阳性物质在肠道的变化.结果烧伤后大鼠肠黏膜血流量下降,EF组下降程度明显低于EPN组(P<0.05~0.01);烧伤后血浆CGRP升高,EF组血浆CGRP在伤后72h明显低于EPN组(P<0.01);肠道CGRP阳性染色物质在烧伤后明显减少(P<0.05~0.01),EF组在伤后24、48h明显多于EPN组(P<0.05);肠黏膜血流量下降和肠肌间丛CGRP下降呈正相关(r=0.72,P<0.05).结论肠道营养在烧伤早期肠道的复苏效应方面较静脉营养优越,伤后肠道CGRP免疫活性物质减少,血浆CGRP免疫活性物质过度增高可能是烧伤后肠道血供减少的重要机制之一,早期肠道营养可能通过调节CGRP而发挥复苏作用. 相似文献
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目的 了解严重烫伤大鼠早期心肌抑制对肝、肾、肠损害及血流灌注的影响.方法 将32只Wistar大鼠按随机数字表分为假伤组、普萘洛尔组、烫伤对照组和烫伤+普萘洛尔组,每组8只.各组大鼠腹腔注射10 g/L戊巴比妥钠(30 mg/kg)麻醉后,假伤组、普萘洛尔组置于37℃水中18 s致假伤,其余2组置于97℃水浴中18 s,造成30%TBSAⅢ度烫伤.伤后30 min按Parkland公式腹腔注射复方乳酸钠林格液(4 mL·kg-1·1%TBSA-1).普萘洛尔组、烫伤+普萘洛尔组补液的同时静脉注射普萘洛尔0.75 mg/kg.伤后6 h以多道生理信号采集仪检测大鼠动脉收缩压(SBP)、动脉舒张压(DBP)、平均动脉压(MAP)以及左心室收缩压(LVSP)、左心室舒张末期压(LVEDP)、左心室压力最大上升/下降速率(±dp/dt max);以激光多普勒血流仪检测肝、肾、肠血流量;同时抽取大鼠静脉血检测血清心肌肌钙蛋白I(cTnI)、总胆汁酸(TBA)、β2-微球蛋白(β2-MG)浓度和二胺氧化酶(DAO)活性.结果 普萘洛尔组除LVEDP外,SBP、DBP、MAP、LVSP、±dp/dt max均低于假伤组(P<0.05).烫伤对照组与假伤组比较,心肌力学指标均下降(P<0.05).烫伤+普萘洛尔组与烫伤对照组比较,各项心肌力学指标均明显下降(P<0.05).与假伤组比较,烫伤对照组肝、肾、肠血流量显著降低(P<0.05);与烫伤对照组比较,烫伤+普萘洛尔组肝、肾、肠血流量降低(P<0.05).烫伤对照组血清cTnI、TBA、β2-MG和DAO值分别为(4.86±0.29)μg/L、(83.6±18.2)μmol/L、(2.75±0.19)mg/L、(1.45±0.09)×103U/L,均高于假伤组[(1.73±0.09)μg/L、(24±2.4)μmol/L、(1.15±0.18)mg/L、(0.87±0.13)×103U/L,P<0.05];与烫伤对照组比较,烫伤+普萘洛尔组血清cTnI(5.95±0.42)μg/L、TBA(125.8±21.3)μmol/L、β2-MG(3.25±0.17)mg/L、DAO(1.83±0.13)×103U/L均明显升高(P<0.05).结论 严重烫伤大鼠早期心肌抑制能加剧肝、肾、肠损害,提示"休克心"可能是严重烧伤后早期肝、肾、肠血流量减少和损害的启动因素之一. 相似文献
10.
早期切痂植皮治疗大鼠电子束辐射烧伤 总被引:2,自引:1,他引:1
电子束引起的皮肤辐射烧伤时有发生 ,临床治疗难度较大。创面植皮是治疗的一个重要手段 ,但对植皮的时机与适应证存在不同意见。本文对大鼠电子束辐射烧伤和热力烧伤早期切痂植皮的疗效进行观察比较 ,为选择辐射烧伤的治疗手段提供参考。1.材料与方法 :(1)选用Wistar大鼠 4 0只 ,雌雄各半 ,体重 2 0 0~ 2 5 0g ,随机分为 3组。A组为辐射烧伤组 ,其中A1组切痂植皮面积为 4cm2 、A2组切痂植皮面积为 6cm2 、A3组不切痂 ;B组为热力烧伤组 ,B1组切痂面积为 4cm2 、B2组不切痂 ;C组为不烧伤的对照组。A、B两组均造成大鼠… 相似文献
11.
烧伤早期对大鼠一氧化氮及合成酶产生的影响 总被引:1,自引:0,他引:1
为探讨一氧化氮(NO)及其合成酶(NOS)在烧伤早期的变化,作者采用雄性Wistar大鼠35%Ⅲ度烫伤模型,于伤前、伤后八个时间点,分别测定血浆、皮肤创面及心、肺、肝、肾、肠五种脏器的NO含量及上述五种脏器内NOS的活性单位。结果表明:(1)烧伤早期(72小时)血浆NO含量值与伤前值比较有降低趋势(P<0.05,或0.01)。(2)皮肤创面中检测的NO含量,伤前、伤后均超过血浆、五种脏器各自相应值的3.8~57.8倍,且伤后3小时显著升高,尔后迅速降低呈低值水平,与伤前比较差异有显著意义(P<0.01)。(3)五种脏器的NO、NOS含量值,伤后升高与降低的趋势基本一致,出现谷值与峰值时间也大致相同。这证实了NOS做为合成NO前体酶含量变化一致性的诊断。而且推测烧伤后皮肤创面可能是导致产生NO的局部场所之一。 相似文献
12.
Objective To investigate the influence of myocardial inhibition on injury to liver, kid-ney and intestine and blood perfusion at early stage in rat with severe burn. Methods Thirty-two healthy male Wistar rats were enrolled in the study and randomly divided into sham scald, propranolol, scald control and scald + propranolol groups, with 8 rats in each group. After intraperitoneal injection of 10 g/L pentobar-bital sodium for anesthesia, rats of the former two groups were sham scalded in a water bath of 37 ℃ for 18 s, while the latter two groups were inflicted with 30% TBSA full-thickness scald in a 97 ℃ water bath for Parkland formula 30 rains after the injury. At the same time, rats in propranolol and scald + propranolol groups received propranolol 0.75 mg/kg intravenously. After 6 hours, parameters of myocardiac mechanics (SBP, DBP, MAP, LVSP, LVEDP, ± dp/dt max) were recorded by the multiple channel physiological signal collecting and processing system ;blood flow of liver, kidney and intestine were detected with the laser doppler flowmetry;the serum contents of cTnI, TBA, beta2-MG and DAO were determined for reflecting in-juries to the heart, liver, kidney and intestine, respectively. Results Myocardiae mechanics parameters, with the exception of LVEDP, were decreased in propranolol group as compared with those in sham group ( P <0.05). All'myoeardiac mechanics parameters in burn control group were lower than those in sham group and higher than those in burn + propranolol group (P<0.05 ). Blood flow of organs showed similar changes in above-mentioned 3 groups (P<0.05). Organ damages as shown in burn control group [cTnI (4.86+0.29) μg/L, TBA (83.6+18.2) μmol/L, β2-MG (2.75±0.19) mg/L, DAO (1.45±0.09)×103 U/L] were more serious than those in sham control group [ cTnI (1.73±0.09) μg/L, TBA (24.5± 2.4)μmoL/L, β2-MG (1.15±0.18) mg/L, DAO (0.87±0.13)×103U/L] , and less serious than those in scald + propranolol group [cTnl 5.95±0.42 μg/L, TBA 125.8±21.3 μmol/L, β2-MG 3.25 ±0.17 mg/L, DAO (1.83±0.13)×103 U/L ] ( P <0.05 ). Conclusions Propranolol can aggravate injury to the liver, kidney and intestine at early stage in rat with severe burn, suggesting that "shock heart" may be one of initial factors in lowering blood flow to the organs, thus inducing injury to them. 相似文献
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Objective To investigate the influence of myocardial inhibition on injury to liver, kid-ney and intestine and blood perfusion at early stage in rat with severe burn. Methods Thirty-two healthy male Wistar rats were enrolled in the study and randomly divided into sham scald, propranolol, scald control and scald + propranolol groups, with 8 rats in each group. After intraperitoneal injection of 10 g/L pentobar-bital sodium for anesthesia, rats of the former two groups were sham scalded in a water bath of 37 ℃ for 18 s, while the latter two groups were inflicted with 30% TBSA full-thickness scald in a 97 ℃ water bath for Parkland formula 30 rains after the injury. At the same time, rats in propranolol and scald + propranolol groups received propranolol 0.75 mg/kg intravenously. After 6 hours, parameters of myocardiac mechanics (SBP, DBP, MAP, LVSP, LVEDP, ± dp/dt max) were recorded by the multiple channel physiological signal collecting and processing system ;blood flow of liver, kidney and intestine were detected with the laser doppler flowmetry;the serum contents of cTnI, TBA, beta2-MG and DAO were determined for reflecting in-juries to the heart, liver, kidney and intestine, respectively. Results Myocardiae mechanics parameters, with the exception of LVEDP, were decreased in propranolol group as compared with those in sham group ( P <0.05). All'myoeardiac mechanics parameters in burn control group were lower than those in sham group and higher than those in burn + propranolol group (P<0.05 ). Blood flow of organs showed similar changes in above-mentioned 3 groups (P<0.05). Organ damages as shown in burn control group [cTnI (4.86+0.29) μg/L, TBA (83.6+18.2) μmol/L, β2-MG (2.75±0.19) mg/L, DAO (1.45±0.09)×103 U/L] were more serious than those in sham control group [ cTnI (1.73±0.09) μg/L, TBA (24.5± 2.4)μmoL/L, β2-MG (1.15±0.18) mg/L, DAO (0.87±0.13)×103U/L] , and less serious than those in scald + propranolol group [cTnl 5.95±0.42 μg/L, TBA 125.8±21.3 μmol/L, β2-MG 3.25 ±0.17 mg/L, DAO (1.83±0.13)×103 U/L ] ( P <0.05 ). Conclusions Propranolol can aggravate injury to the liver, kidney and intestine at early stage in rat with severe burn, suggesting that "shock heart" may be one of initial factors in lowering blood flow to the organs, thus inducing injury to them. 相似文献
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Objective To investigate the influence of myocardial inhibition on injury to liver, kid-ney and intestine and blood perfusion at early stage in rat with severe burn. Methods Thirty-two healthy male Wistar rats were enrolled in the study and randomly divided into sham scald, propranolol, scald control and scald + propranolol groups, with 8 rats in each group. After intraperitoneal injection of 10 g/L pentobar-bital sodium for anesthesia, rats of the former two groups were sham scalded in a water bath of 37 ℃ for 18 s, while the latter two groups were inflicted with 30% TBSA full-thickness scald in a 97 ℃ water bath for Parkland formula 30 rains after the injury. At the same time, rats in propranolol and scald + propranolol groups received propranolol 0.75 mg/kg intravenously. After 6 hours, parameters of myocardiac mechanics (SBP, DBP, MAP, LVSP, LVEDP, ± dp/dt max) were recorded by the multiple channel physiological signal collecting and processing system ;blood flow of liver, kidney and intestine were detected with the laser doppler flowmetry;the serum contents of cTnI, TBA, beta2-MG and DAO were determined for reflecting in-juries to the heart, liver, kidney and intestine, respectively. Results Myocardiae mechanics parameters, with the exception of LVEDP, were decreased in propranolol group as compared with those in sham group ( P <0.05). All'myoeardiac mechanics parameters in burn control group were lower than those in sham group and higher than those in burn + propranolol group (P<0.05 ). Blood flow of organs showed similar changes in above-mentioned 3 groups (P<0.05). Organ damages as shown in burn control group [cTnI (4.86+0.29) μg/L, TBA (83.6+18.2) μmol/L, β2-MG (2.75±0.19) mg/L, DAO (1.45±0.09)×103 U/L] were more serious than those in sham control group [ cTnI (1.73±0.09) μg/L, TBA (24.5± 2.4)μmoL/L, β2-MG (1.15±0.18) mg/L, DAO (0.87±0.13)×103U/L] , and less serious than those in scald + propranolol group [cTnl 5.95±0.42 μg/L, TBA 125.8±21.3 μmol/L, β2-MG 3.25 ±0.17 mg/L, DAO (1.83±0.13)×103 U/L ] ( P <0.05 ). Conclusions Propranolol can aggravate injury to the liver, kidney and intestine at early stage in rat with severe burn, suggesting that "shock heart" may be one of initial factors in lowering blood flow to the organs, thus inducing injury to them. 相似文献
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Objective To investigate the influence of myocardial inhibition on injury to liver, kid-ney and intestine and blood perfusion at early stage in rat with severe burn. Methods Thirty-two healthy male Wistar rats were enrolled in the study and randomly divided into sham scald, propranolol, scald control and scald + propranolol groups, with 8 rats in each group. After intraperitoneal injection of 10 g/L pentobar-bital sodium for anesthesia, rats of the former two groups were sham scalded in a water bath of 37 ℃ for 18 s, while the latter two groups were inflicted with 30% TBSA full-thickness scald in a 97 ℃ water bath for Parkland formula 30 rains after the injury. At the same time, rats in propranolol and scald + propranolol groups received propranolol 0.75 mg/kg intravenously. After 6 hours, parameters of myocardiac mechanics (SBP, DBP, MAP, LVSP, LVEDP, ± dp/dt max) were recorded by the multiple channel physiological signal collecting and processing system ;blood flow of liver, kidney and intestine were detected with the laser doppler flowmetry;the serum contents of cTnI, TBA, beta2-MG and DAO were determined for reflecting in-juries to the heart, liver, kidney and intestine, respectively. Results Myocardiae mechanics parameters, with the exception of LVEDP, were decreased in propranolol group as compared with those in sham group ( P <0.05). All'myoeardiac mechanics parameters in burn control group were lower than those in sham group and higher than those in burn + propranolol group (P<0.05 ). Blood flow of organs showed similar changes in above-mentioned 3 groups (P<0.05). Organ damages as shown in burn control group [cTnI (4.86+0.29) μg/L, TBA (83.6+18.2) μmol/L, β2-MG (2.75±0.19) mg/L, DAO (1.45±0.09)×103 U/L] were more serious than those in sham control group [ cTnI (1.73±0.09) μg/L, TBA (24.5± 2.4)μmoL/L, β2-MG (1.15±0.18) mg/L, DAO (0.87±0.13)×103U/L] , and less serious than those in scald + propranolol group [cTnl 5.95±0.42 μg/L, TBA 125.8±21.3 μmol/L, β2-MG 3.25 ±0.17 mg/L, DAO (1.83±0.13)×103 U/L ] ( P <0.05 ). Conclusions Propranolol can aggravate injury to the liver, kidney and intestine at early stage in rat with severe burn, suggesting that "shock heart" may be one of initial factors in lowering blood flow to the organs, thus inducing injury to them. 相似文献
16.
Objective To investigate the influence of myocardial inhibition on injury to liver, kid-ney and intestine and blood perfusion at early stage in rat with severe burn. Methods Thirty-two healthy male Wistar rats were enrolled in the study and randomly divided into sham scald, propranolol, scald control and scald + propranolol groups, with 8 rats in each group. After intraperitoneal injection of 10 g/L pentobar-bital sodium for anesthesia, rats of the former two groups were sham scalded in a water bath of 37 ℃ for 18 s, while the latter two groups were inflicted with 30% TBSA full-thickness scald in a 97 ℃ water bath for Parkland formula 30 rains after the injury. At the same time, rats in propranolol and scald + propranolol groups received propranolol 0.75 mg/kg intravenously. After 6 hours, parameters of myocardiac mechanics (SBP, DBP, MAP, LVSP, LVEDP, ± dp/dt max) were recorded by the multiple channel physiological signal collecting and processing system ;blood flow of liver, kidney and intestine were detected with the laser doppler flowmetry;the serum contents of cTnI, TBA, beta2-MG and DAO were determined for reflecting in-juries to the heart, liver, kidney and intestine, respectively. Results Myocardiae mechanics parameters, with the exception of LVEDP, were decreased in propranolol group as compared with those in sham group ( P <0.05). All'myoeardiac mechanics parameters in burn control group were lower than those in sham group and higher than those in burn + propranolol group (P<0.05 ). Blood flow of organs showed similar changes in above-mentioned 3 groups (P<0.05). Organ damages as shown in burn control group [cTnI (4.86+0.29) μg/L, TBA (83.6+18.2) μmol/L, β2-MG (2.75±0.19) mg/L, DAO (1.45±0.09)×103 U/L] were more serious than those in sham control group [ cTnI (1.73±0.09) μg/L, TBA (24.5± 2.4)μmoL/L, β2-MG (1.15±0.18) mg/L, DAO (0.87±0.13)×103U/L] , and less serious than those in scald + propranolol group [cTnl 5.95±0.42 μg/L, TBA 125.8±21.3 μmol/L, β2-MG 3.25 ±0.17 mg/L, DAO (1.83±0.13)×103 U/L ] ( P <0.05 ). Conclusions Propranolol can aggravate injury to the liver, kidney and intestine at early stage in rat with severe burn, suggesting that "shock heart" may be one of initial factors in lowering blood flow to the organs, thus inducing injury to them. 相似文献
17.
Objective To investigate the influence of myocardial inhibition on injury to liver, kid-ney and intestine and blood perfusion at early stage in rat with severe burn. Methods Thirty-two healthy male Wistar rats were enrolled in the study and randomly divided into sham scald, propranolol, scald control and scald + propranolol groups, with 8 rats in each group. After intraperitoneal injection of 10 g/L pentobar-bital sodium for anesthesia, rats of the former two groups were sham scalded in a water bath of 37 ℃ for 18 s, while the latter two groups were inflicted with 30% TBSA full-thickness scald in a 97 ℃ water bath for Parkland formula 30 rains after the injury. At the same time, rats in propranolol and scald + propranolol groups received propranolol 0.75 mg/kg intravenously. After 6 hours, parameters of myocardiac mechanics (SBP, DBP, MAP, LVSP, LVEDP, ± dp/dt max) were recorded by the multiple channel physiological signal collecting and processing system ;blood flow of liver, kidney and intestine were detected with the laser doppler flowmetry;the serum contents of cTnI, TBA, beta2-MG and DAO were determined for reflecting in-juries to the heart, liver, kidney and intestine, respectively. Results Myocardiae mechanics parameters, with the exception of LVEDP, were decreased in propranolol group as compared with those in sham group ( P <0.05). All'myoeardiac mechanics parameters in burn control group were lower than those in sham group and higher than those in burn + propranolol group (P<0.05 ). Blood flow of organs showed similar changes in above-mentioned 3 groups (P<0.05). Organ damages as shown in burn control group [cTnI (4.86+0.29) μg/L, TBA (83.6+18.2) μmol/L, β2-MG (2.75±0.19) mg/L, DAO (1.45±0.09)×103 U/L] were more serious than those in sham control group [ cTnI (1.73±0.09) μg/L, TBA (24.5± 2.4)μmoL/L, β2-MG (1.15±0.18) mg/L, DAO (0.87±0.13)×103U/L] , and less serious than those in scald + propranolol group [cTnl 5.95±0.42 μg/L, TBA 125.8±21.3 μmol/L, β2-MG 3.25 ±0.17 mg/L, DAO (1.83±0.13)×103 U/L ] ( P <0.05 ). Conclusions Propranolol can aggravate injury to the liver, kidney and intestine at early stage in rat with severe burn, suggesting that "shock heart" may be one of initial factors in lowering blood flow to the organs, thus inducing injury to them. 相似文献
18.
Objective To investigate the influence of myocardial inhibition on injury to liver, kid-ney and intestine and blood perfusion at early stage in rat with severe burn. Methods Thirty-two healthy male Wistar rats were enrolled in the study and randomly divided into sham scald, propranolol, scald control and scald + propranolol groups, with 8 rats in each group. After intraperitoneal injection of 10 g/L pentobar-bital sodium for anesthesia, rats of the former two groups were sham scalded in a water bath of 37 ℃ for 18 s, while the latter two groups were inflicted with 30% TBSA full-thickness scald in a 97 ℃ water bath for Parkland formula 30 rains after the injury. At the same time, rats in propranolol and scald + propranolol groups received propranolol 0.75 mg/kg intravenously. After 6 hours, parameters of myocardiac mechanics (SBP, DBP, MAP, LVSP, LVEDP, ± dp/dt max) were recorded by the multiple channel physiological signal collecting and processing system ;blood flow of liver, kidney and intestine were detected with the laser doppler flowmetry;the serum contents of cTnI, TBA, beta2-MG and DAO were determined for reflecting in-juries to the heart, liver, kidney and intestine, respectively. Results Myocardiae mechanics parameters, with the exception of LVEDP, were decreased in propranolol group as compared with those in sham group ( P <0.05). All'myoeardiac mechanics parameters in burn control group were lower than those in sham group and higher than those in burn + propranolol group (P<0.05 ). Blood flow of organs showed similar changes in above-mentioned 3 groups (P<0.05). Organ damages as shown in burn control group [cTnI (4.86+0.29) μg/L, TBA (83.6+18.2) μmol/L, β2-MG (2.75±0.19) mg/L, DAO (1.45±0.09)×103 U/L] were more serious than those in sham control group [ cTnI (1.73±0.09) μg/L, TBA (24.5± 2.4)μmoL/L, β2-MG (1.15±0.18) mg/L, DAO (0.87±0.13)×103U/L] , and less serious than those in scald + propranolol group [cTnl 5.95±0.42 μg/L, TBA 125.8±21.3 μmol/L, β2-MG 3.25 ±0.17 mg/L, DAO (1.83±0.13)×103 U/L ] ( P <0.05 ). Conclusions Propranolol can aggravate injury to the liver, kidney and intestine at early stage in rat with severe burn, suggesting that "shock heart" may be one of initial factors in lowering blood flow to the organs, thus inducing injury to them. 相似文献
19.
Objective To investigate the influence of myocardial inhibition on injury to liver, kid-ney and intestine and blood perfusion at early stage in rat with severe burn. Methods Thirty-two healthy male Wistar rats were enrolled in the study and randomly divided into sham scald, propranolol, scald control and scald + propranolol groups, with 8 rats in each group. After intraperitoneal injection of 10 g/L pentobar-bital sodium for anesthesia, rats of the former two groups were sham scalded in a water bath of 37 ℃ for 18 s, while the latter two groups were inflicted with 30% TBSA full-thickness scald in a 97 ℃ water bath for Parkland formula 30 rains after the injury. At the same time, rats in propranolol and scald + propranolol groups received propranolol 0.75 mg/kg intravenously. After 6 hours, parameters of myocardiac mechanics (SBP, DBP, MAP, LVSP, LVEDP, ± dp/dt max) were recorded by the multiple channel physiological signal collecting and processing system ;blood flow of liver, kidney and intestine were detected with the laser doppler flowmetry;the serum contents of cTnI, TBA, beta2-MG and DAO were determined for reflecting in-juries to the heart, liver, kidney and intestine, respectively. Results Myocardiae mechanics parameters, with the exception of LVEDP, were decreased in propranolol group as compared with those in sham group ( P <0.05). All'myoeardiac mechanics parameters in burn control group were lower than those in sham group and higher than those in burn + propranolol group (P<0.05 ). Blood flow of organs showed similar changes in above-mentioned 3 groups (P<0.05). Organ damages as shown in burn control group [cTnI (4.86+0.29) μg/L, TBA (83.6+18.2) μmol/L, β2-MG (2.75±0.19) mg/L, DAO (1.45±0.09)×103 U/L] were more serious than those in sham control group [ cTnI (1.73±0.09) μg/L, TBA (24.5± 2.4)μmoL/L, β2-MG (1.15±0.18) mg/L, DAO (0.87±0.13)×103U/L] , and less serious than those in scald + propranolol group [cTnl 5.95±0.42 μg/L, TBA 125.8±21.3 μmol/L, β2-MG 3.25 ±0.17 mg/L, DAO (1.83±0.13)×103 U/L ] ( P <0.05 ). Conclusions Propranolol can aggravate injury to the liver, kidney and intestine at early stage in rat with severe burn, suggesting that "shock heart" may be one of initial factors in lowering blood flow to the organs, thus inducing injury to them. 相似文献
20.
Objective To investigate the influence of myocardial inhibition on injury to liver, kid-ney and intestine and blood perfusion at early stage in rat with severe burn. Methods Thirty-two healthy male Wistar rats were enrolled in the study and randomly divided into sham scald, propranolol, scald control and scald + propranolol groups, with 8 rats in each group. After intraperitoneal injection of 10 g/L pentobar-bital sodium for anesthesia, rats of the former two groups were sham scalded in a water bath of 37 ℃ for 18 s, while the latter two groups were inflicted with 30% TBSA full-thickness scald in a 97 ℃ water bath for Parkland formula 30 rains after the injury. At the same time, rats in propranolol and scald + propranolol groups received propranolol 0.75 mg/kg intravenously. After 6 hours, parameters of myocardiac mechanics (SBP, DBP, MAP, LVSP, LVEDP, ± dp/dt max) were recorded by the multiple channel physiological signal collecting and processing system ;blood flow of liver, kidney and intestine were detected with the laser doppler flowmetry;the serum contents of cTnI, TBA, beta2-MG and DAO were determined for reflecting in-juries to the heart, liver, kidney and intestine, respectively. Results Myocardiae mechanics parameters, with the exception of LVEDP, were decreased in propranolol group as compared with those in sham group ( P <0.05). All'myoeardiac mechanics parameters in burn control group were lower than those in sham group and higher than those in burn + propranolol group (P<0.05 ). Blood flow of organs showed similar changes in above-mentioned 3 groups (P<0.05). Organ damages as shown in burn control group [cTnI (4.86+0.29) μg/L, TBA (83.6+18.2) μmol/L, β2-MG (2.75±0.19) mg/L, DAO (1.45±0.09)×103 U/L] were more serious than those in sham control group [ cTnI (1.73±0.09) μg/L, TBA (24.5± 2.4)μmoL/L, β2-MG (1.15±0.18) mg/L, DAO (0.87±0.13)×103U/L] , and less serious than those in scald + propranolol group [cTnl 5.95±0.42 μg/L, TBA 125.8±21.3 μmol/L, β2-MG 3.25 ±0.17 mg/L, DAO (1.83±0.13)×103 U/L ] ( P <0.05 ). Conclusions Propranolol can aggravate injury to the liver, kidney and intestine at early stage in rat with severe burn, suggesting that "shock heart" may be one of initial factors in lowering blood flow to the organs, thus inducing injury to them. 相似文献