卵巢癌患者血清糖类抗原125、糖类抗原153、糖类抗原19-9水平与临床意义

目的 探讨卵巢癌患者血清糖类抗原（CA）125、CA153、CA19-9水平与临床意义。方法 将213例卵巢占位性病变患者根据病理结果分为病例组（卵巢癌）107例和对照组（卵巢良性病变）106例。比较两组患者的临床特征和血清CA125、CA153、CA19-9水平;比较不同国际妇产科联盟（FIGO）分期卵巢癌患者血清CA125、CA153、CA19-9水平;比较血清CA125、CA153、CA19-9单独及联合检测对卵巢癌的诊断价值。结果 病例组患者血清CA125、CA153、CA19-9水平均明显高于对照组,差异均有统计学意义（P﹤0.01）。Ⅲ～Ⅳ期卵巢癌患者血清CA125、CA153、CA19-9水平均明显高于Ⅰ～Ⅱ期患者,差异均有统计学意义（P﹤0.01）。CA125、CA153与CA19-9均对卵巢癌有一定的诊断价值,而三者联合检测诊断卵巢癌的灵敏度高于三者单独检测。病理诊断Ⅰ～Ⅱ期卵巢癌48例,CA125、CA153与CA19-9联合检测诊断Ⅰ～Ⅱ期卵巢癌40例,三者联合检测诊断Ⅰ～Ⅱ期卵巢癌的灵敏度为0.83(40/48)。结论 血清CA125、CA153与CA19-9联...     

血清糖类抗原19-9、糖类抗原125和人附睾蛋白4水平检测对老年卵巢癌患者的诊断和鉴别诊断价值

目的 分析血清糖类抗原19-9(CA19-9)、糖类抗原125(CA125)和人附睾蛋白4(HE4)水平检测对老年卵巢癌患者的诊断和鉴别诊断价值.方法 纳入51例老年卵巢癌患者(卵巢癌组)、33例卵巢良性病变患者(良性病变组)、30例健康体检女性(对照组).比较3组研究对象血清CA19-9、CA125和HE4水平,并比...     

间皮素、糖类抗原125单独及联合检测对卵巢癌的诊断价值及临床意义

目的 探讨间皮素、糖类抗原125(CA125)单独及联合检测对卵巢癌的诊断价值及临床意义。方法选取58例卵巢癌患者（卵巢癌组）,其中30例患者行紫杉醇联合铂类药物化疗,选取45例健康体检者为对照组。检测所有受试者血清、尿液间皮素水平,检测卵巢癌患者血清CA125水平,化疗患者化疗前后行影像学检查。绘制受试者工作特征（ROC）曲线,计算曲线下面积（AUC）,评估间皮素、CA125单独及联合检测对卵巢癌的诊断价值。结果 卵巢癌组患者血清、尿液间皮素水平均明显高于对照组（P﹤0.01）;化疗后,卵巢癌化疗患者血清间皮素水平明显高于本组化疗前（P﹤0.01）。Ⅱ期与Ⅲ～Ⅳ期卵巢癌患者血清、尿液间皮素水平比较,差异均无统计学意义（P﹥0.05）。尿液间皮素诊断卵巢癌的AUC为0.861(95%CI:0.794～0.929),高于血清间皮素的0.701,尿液间皮素诊断卵巢癌的特异度较高,诊断价值较高。血清间皮素+CA125联合检测对卵巢癌的阳性检出率为75.9%,高于二者单独检测（P﹤0.05）。化疗后,卵巢癌患者血清CA125水平明显低于本组化疗前,可测量病灶长径明显短于本组化疗前（P﹤0.01...     

血清癌胚抗原联合糖类抗原19-9检测对胰腺癌筛查的临床价值评估

目的 研究血清癌胚抗原(CEA)联合糖类抗原19-9(CA19-9)检测对胰腺癌筛查的临床价值.方法 将103例胰腺癌患者作为胰腺癌组,选取同期收治的105例胰腺炎患者以及健康体检的97例健康人群作为胰腺炎组及健康对照组.对比治疗前3组血清CEA、CA19-9水平,以及手术前后胰腺癌患者血清CEA、CA19-9水平;对比血清CEA、CA19-9单独及联合检测诊断胰腺癌的灵敏度、准确度、特异度.结果 胰腺癌组与胰腺炎组患者血清CEA、CA19-9水平均高于健康对照组,胰腺癌组患者血清CEA、CA19-9水平均高于胰腺炎组,差异均有统计学意义(P﹤0.05).血清CEA联合CA19-9检测诊断胰腺癌的灵敏度明显优于CEA、CA19-9单一检测.术后3个月,胰腺癌患者血清CEA、CA19-9水平均明显低于手术前,差异均有统计学意义(P﹤0.01).结论 在胰腺癌的筛查过程中,采用血清CEA联合CA19-9检测效果显著,两者联合检测对胰腺癌诊断有较高的灵敏度、准确度,有助于提高疾病诊断率.     

糖类抗原125、糖类抗原15-3、糖类抗原19-9和人附睾蛋白4单独及联合检测对卵巢癌的诊断价值

目的研究糖类抗原125(CA125)、CA15-3、CA19-9和人附睾蛋白4(HE4)单独及联合检测对卵巢癌的诊断价值,探讨最优的联合诊断方案。方法选取90例卵巢癌患者、90例良性卵巢疾病患者及90同期健康体检者,分别作为肿瘤组、良性组和健康组。比较3组受试者血清CA125、CA15-3、CA19-9和HE4水平及阳性率,比较上述四种肿瘤标志物单独及联合检测对卵巢癌的诊断灵敏度、特异度及准确度。结果肿瘤组患者CA125、CA15-3、CA19-9和HE4水平及阳性率均高于良性组患者和健康组受试者,良性组患者血清CA125、CA15-3和CA19-9水平均高于健康组受试者,良性组患者血清CA125、CA15-3、CA19-9和HE4阳性率均高于健康组受试者,差异均有统计学意义(P﹤0.05)。CA125+CA15-3+HE4、CA125+CA15-3+CA19-9+HE4诊断卵巢癌灵敏度最高,均为91.11%。HE4单独诊断卵巢癌的特异度最高,为94.44%。CA125+CA15-3+HE4诊断卵巢癌的准确度最高,为85.93%。结论CA125+CA15-3+HE4三项联合检测可提高卵巢癌诊断效率,对临床诊断卵巢癌有参考价值。     

血清miiRNA-196a联合糖类抗原125、糖类抗原19-9对宫颈癌的诊断价值

目的 探讨血清miRNA-196a联合糖类抗原19-9(CA19-9)及CA125诊断宫颈癌的临床价值.方法 选取180例宫颈癌患者(宫颈癌组)、50例宫颈良性肿瘤患者(良性组)及50例健康体检者(对照组)为研究对象,比较不同临床分期宫颈癌患者血清miRNA-196a、CA125、CA19-9水平,比较3组研究对象血清...     

癌胚抗原、糖类抗原125、糖类抗原19-9和糖类抗原72-4在胃肠道间质瘤临床诊断中的应用价值分析

目的 探讨癌胚抗原(CEA)、糖类抗原125(CA125)、糖类抗原19-9(CA19-9)和糖类抗原72-4(CA72-4)在胃肠道间质瘤(GIST)诊断中的应用价值。方法 将2019年1月至2021年6月安阳市肿瘤医院收治的初诊未治的40例GIST患者和同期体检的40例健康志愿者,分别作为GIST组和对照组。比较2组研究对象血清CEA、CA125、CA19-9和CA72-4水平,并分析其单独或联合检测的诊断效能。结果 与对照组比较,GIST组患者血清中CEA、CA125、CA19-9和CA72-4水平显著升高(t=24.177,P<0.001;t=8.921,P=0.032;t=14.215,P=0.008;t=5.663,P=0.041)。与对照组比较,GIST组患者血清CEA、CA125、CA19-9和CA72-4阳性率也显著升高(χ²=28.256,P<0.001;χ²=26.332,P<0.001;χ²=29.034,P<0.001;χ²=24.511,P<...     

血清α-羟丁酸脱氢酶联合糖类抗原125检测在卵巢癌诊断中的应用价值

目的探讨卵巢癌患者血清α-羟丁酸脱氢酶、糖类抗原125(CA125)在卵巢癌中的表达情况,分析二者联合检测对卵巢癌的诊断价值。方法选取40例卵巢癌患者、35例卵巢良性病变患者和40例健康者,分别命名为卵巢癌组、卵巢良性病变组、健康对照组。检测血清α-羟丁酸脱氢酶、CA125水平,探讨血清α-羟丁酸脱氢酶水平与卵巢癌患者病理特征的关系。绘制受试者工作特征(ROC)曲线,分析血清α-羟丁酸脱氢酶联合CA125检测对卵巢癌的诊断效能。结果卵巢癌组和卵巢良性病变组患者的血清CA125、α-羟丁酸脱氢酶水平均高于健康对照组受试者,卵巢癌组患者的血清CA125水平高于卵巢良性病变组患者(P﹤0.05)。不同分化程度、国际妇产科联盟(FIGO)分期和淋巴结转移情况的卵巢癌患者的血清α-羟丁酸脱氢酶水平比较,差异均有统计学意义(P﹤0.01)。ROC曲线分析结果显示,血清α-羟丁酸脱氢酶联合CA125检测鉴别诊断卵巢癌与卵巢良性病变的曲线下面积为0.834(95%CI:0.743~0.924),灵敏度和特异度分别为85.46%和90.02%;鉴别诊断卵巢癌与健康者的曲线下面积为0.958(95%CI:0.919~0.997),灵敏度和特异度分别为89.47%和95.83%。结论卵巢癌患者的血清α-羟丁酸脱氢酶和CA125水平较高,有一定的卵巢癌诊断价值,二者联合检测可提高对卵巢癌的诊断效能。     

肠镜联合血清肿瘤标志物检测对结直肠癌的诊断价值分析

目的 分析肠镜联合血清肿瘤标志物检测对结直肠癌的诊断价值.方法 将103例结直肠癌患者纳入恶性组,以同期收治的103例结直肠良性病变患者作为良性组,比较两组患者血清肿瘤标志物[糖类抗原72-4(CA72-4)、糖类抗原19-9(CA19-9)、癌胚抗原(CEA)]水平,全部患者均接受肠镜检查;以病理检查结果为金标准,采用受试者工作特征(ROC)曲线分析肠镜、血清肿瘤标志物单独及联合检测对结直肠癌的诊断价值,分析各项检测方法诊断结果与金标准的一致性,计算诊断效能.结果 恶性组患者血清CA19-9、CEA、CA72-4水平均明显高于良性组,差异均有统计学意义(P﹤0.01).肠镜与血清肿瘤标志物联合诊断结直肠癌的各项诊断效能均高于各项单独应用,灵敏度为89.32％,特异度为99.03％,准确度为94.17％;各项诊断结果与金标准的一致性均为良(0.6﹤Kappa值≤0.8,P﹤0.01).肠镜具有中等诊断价值[曲线下面积(AUC)=0.883,P﹤0.01];3项血清肿瘤标志物均具有中等诊断价值,其中CA19-9最高(AUC=0.854,P﹤0.01);肠镜与血清肿瘤标志物联合应用具有较高的诊断价值(AUC=0.942,P﹤0.01).结论 肠镜联合血清肿瘤标志物检测对结直肠癌具有较高的诊断价值.     

血清癌胚抗原、糖类抗原125、糖类抗原153联合检测对乳腺癌的诊断价值

目的 探讨血清癌胚抗原（CEA）、糖类抗原125(CA125)、糖类抗原153(CA153)联合检测对乳腺癌的诊断价值。方法 选取106例乳腺癌患者和72例乳腺良性疾病患者,分别作为恶性组和良性组。比较两组患者的血清CEA、CA125、CA153水平,绘制受试者工作特征（ROC）曲线,分析血清CEA、CA125、CA153单独及三者联合检测对乳腺癌的诊断价值。结果 恶性组患者的血清CEA、CA125、CA153水平均明显高于良性组,差异均有统计学意义（P<0.01）。CA153对乳腺癌具有较高诊断价值,CEA、CA125对乳腺癌具有中等诊断价值,三者联合检测对乳腺癌具有较高诊断价值,且诊断价值高于各指标单独检测。结论 血清CEA、CA125、CA153联合检测对乳腺癌具有较高诊断价值,可用于乳腺癌筛查。     

Pancreatic cancer-associated new carbohydrate antigen

Antigen T (Thomsen-Friedenreich), a precursor of blood group MN antigens, with the determinant of a carbohydrate (Gal-GalNac), antigen Lewisx (Lex), a trisaccharide found on Type 2 blood group oligosaccharide chains, Sialo-Lex, the derivatives: of Lex and Lewisy (Ley), a difucosylated tetrasaccharide have, behaved as the oncodevelopment tumor-associated antigens in human colon. In the present study, using monoclonal antibodies in immunohistochemical method, the expression and distribution of these antigens in pancreatic cancer and normal pancreatic tissue were observed and compared. It was found that monoclonal antibody AH8-258 derived against antigen T, SSEA-1 and FH-4 derived against short and long chain antigens Lex, FH-6 and IB 9 derived against Sialo-Lex antigen preferentially stained most pancreatic cancer tissues but rarely the normal pancreatic tissues. However, monoclonal antibodies AH-6, KH-1 and CC-1, derived against antigen Ley, stained the majority of tissues regardless of being malignant or normal and were not able to differentiate cancer from the normal tissues. Antigens T, Lex, Sialo-Lex and Ley were also expressed in chronic pancreatitis but the chance of monoclonal antibodies staining in these tissues (18 approximately 36%) was markedly lower than the staining in cancer tissues (54 approximately 77%) except Ley. It is suggested that in human pancreas, haptens T, Lex and Sialo-Lex, the oncodevelopmental cancer-associated antigens, are highly specific markers for malignancy and likely helpful in the early diagnosis of pancreatic cancer.     

CA125联合CA199检测在妇科肿瘤诊断中的应用

目的探讨糖类抗原125(CA125)联合糖类抗原199(CA199)检测在妇科肿瘤诊断中的应用效果。方法选取2011年10月至2014年10月间收治的111例良性肿瘤妇科患者为良性肿瘤组,111例恶性肿瘤妇科患者为恶性肿瘤组,同时选取111例正常体检妇女为对照组,均行CA125、CA199检测,分析其在妇科肿瘤诊断中的诊断价值。结果良性肿瘤组患者CA125、CA199水平均明显高于对照组,差异有统计学意义(P<0.05)。恶性肿瘤组患者CA125、CA199水平均明显高于对照组和良性肿瘤组,差异有统计学意义(P<0.05)。CA125在<35 U/mol、35~70 U/mol时,恶性肿瘤组患者CA125检出率均明显低于良性肿瘤组,差异有统计学意义(P<0.05)。CA125在71~140 U/mol、141~280 U/mol、>280 U/mol时,恶性肿瘤组CA125检出率均明显高于良性肿瘤组,差异有统计学意义(P<0.05)。无论是CA125检测还是CA125+CA199联合检测,CA125>140 U/mol的敏感性均明显高于CA125≤140 U/mol,CA125>140 U/mol的特异性均明显低于CA125≤140 U/mol,差异有统计学意义(P<0.05)。CA125+CA199联合检测的敏感性高于单独CA125检测,特异性低于CA125检测,差异有统计学意义(P<0.05)。良性肿瘤组的CA125、CA125+CA199检测的ROC曲线下面积分别为0.841和0.879;恶性肿瘤组的CA125、CA125+CA199检测的ROC曲线下面积分别为0.901和0.967。结论 CA125联合CA199检测可提高CA125诊断的敏感性,具有较高的诊断价值。     

Clinical investigation of carbohydrate antigen CA-50

The CA-50 enzyme immunoassay kit (EIA kit) that has been developed with the use of C-50 monoclonal antibody prepared by L. Lindholm et al. was evaluated for diagnosis of human cancer. The levels of CA-50 in the sera were determined using this kit supplied from Mitsui Pharmaceuticals, Inc. Co. in 759 healthy donors, 728 patients with benign disease and 1,263 untreated patients with cancer. A CA-50 concentration of 40 U/ml of serum was used as the cut-off value. Patients with pancreatic cancer and patients with bile duct cancer had high positive incidence of 75% and 68%, respectively, compared with a low positive incidence of under 40% in patients with other cancers. On the other hand, positive rates in patients with benign disease were as low as 13%. Comparison of the serum levels of CA-50 with CA19-9 in the same samples did not exhibit complete positive correlation in patients with pancreatic cancer, patients with bile duct cancer and patients with liver cancer. These findings indicated that C-50 antibody reacted with two epitopes of CA19-9 and sialosyllactotetraose. From the above results, the usefulness of CA-50 as a tumor marker for pancreatic cancer and bile duct cancer was recognized with this EIA kit.     

糖类抗原125、糖类抗原153水平与乳腺癌患者临床特征及预后的关系

目的 探讨糖类抗原125(CA125)、糖类抗原153(CA153)水平与乳腺癌患者临床特征及预后的关系.方法 选取220例乳腺癌患者(乳腺癌组)及同期收治的100例健康体检者(健康对照组),比较两组患者血清CA125、CA153水平,比较不同临床特征乳腺癌患者血清CA125、CA153阳性率,并对乳腺癌患者进行为期5...     

Establishment of a human monoclonal antibody to Hanganutziu-Deicher antigen as a tumor-associated carbohydrate antigen

We have established a human-human hybridoma producing a monoclonal antibody against a tumor-associated carbohydrate-specific antigen, Hanganutziu-Deicher (HD) antigen. Human spleen lymphocytes from a patient with esophageal varices complicated with liver cirrhosis were cultured in serum-free medium and co-stimulated with both anti-human mu-chain antibodies and supernatants of concanavalin A-stimulated human spleen cell culture (ConA sup). The activated lymphocytes were subsequently primed in vitro with particulate HD3 antigen and fused with a parent hybrid myeloma cell line, KR-12. A hybridoma, 1F43E31G7 produced anti-HD human monoclonal antibody (IgM lambda). This monoclonal antibody reacted strongly with N-glycolylneuraminyl alpha 2-3 lactosylceramide (HD3) and slightly with N-glycolylneuraminyl alpha 2-3 lactoneotetraosylceramide (HD5), but did not react with N-glycolylneuraminyl alpha 2-3 lactoneohexaosylceramide (HD7), N-acetylneuraminyl alpha 2-3 lactosylceramide (GM3) and other derivatives of HD3 prepared by chemical modification of the sialic acid residue of HD3, which indicates that the monoclonal antibody is directed precisely toward the terminal sialic acid and whole structure of HD3.     

Role of carcinoembryonic antigen in the progression of colon cancer cells that express carbohydrate antigen

Carcinoembryonic antigen (CEA) has been reported to promote the metastatic potential in some experimental tumors. Adhesion molecules are known to play an important role in the process of metastasis. Cytokines, including interleukin 1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha), which are produced by Kupffer cells, induce endothelial cells to express adhesion molecules. As a result, the present study was designed to investigate whether the interaction between CEA and Kupffer cells accelerated the metastatic potential of tumors in the liver. Kupffer cells isolated from the liver of male BALB/c mice were cultured with CEA, either with or without the addition of a cytokine inhibitor. The levels of IL-1beta and TNF-alpha were examined in a culture medium. An adhesion assay of colon cancer cell lines to human umbilical vein endothelial cells was also performed. When CEA was added to the Kupffer cell culture medium, cytokines were produced. Elevated levels of cytokines appeared to lead to increased rates of adhesion of cancer cells to endothelial cells. However, these phenomena were blocked by the addition of cytokine inhibitors. CEA stimulated Kupffer cells to produce cytokines. An elevated number of cytokines have been proven to promote the expression of adhesion molecules in endothelial cells. These processes are therefore considered to contribute to the metastasis of malignant cells to the liver. These results suggest that cytokine inhibitors may therefore play an important role in the inhibition of hepatic metastasis.     

Study on carbohydrate antigen NCC-ST-439 in breast cancer

We evaluated the clinical significance of serum NCC-ST-439 (ST439) in sera from 20 healthy women, 8 patients with benign breast disease and 105 patients with breast cancer (79 primary, 26 recurrent) by using enzyme immuno-assay (EIA). No false positive case was noted in the measuring of ST439 for healthy women and patients with benign breast disease. The positive rates of ST439 were 23% (18/79) in primary breast cancers and 50% (13/26) in recurrent breast cancers. The serum levels of ST439 in stage I breast cancer was significantly higher (p less than 0.05) than those in healthy women, but there was no significant difference among each stage. The serum levels of ST439 were not significantly different among the subsets such as T, n, m and histological types. The high levels of serum ST439 were observed in two cases with mucinous carcinoma. Although there were no relation between ST439 and receptor status, the higher serum levels of ST439 were observed in postmenopausal patients than premenopausal ones. The positive frequency of serum ST439 in stage I and II breast cancers was higher than that of CEA, TPA or CA15-3, while the positive rate in recurrent breast cancer was the lowest among 4 tumor markers. These results suggest that ST439 is a useful tumor marker for not only detecting the recurrence of breast cancer but also diagnosing primary breast cancer in early stage.