The effects of Psidium guajava Linn. (Myrtaceae) leaf chloroform extract on some hematological and biochemical parameters in mice

Psidium guajava Linn. leaf extract is used in Nigerian ethnomedicine to treat many disorders such as diarrhea, cough, gastroenteritis, gastric pain, and inflammatory disorders. The effects of the chlorofrom extract of the leaves on some hematological and biochemical parameters were investigated in mice. The animals were divided into three groups of eight animals per group. The first group served as the control, while the second and the third groups were given orally 24.3 and 45.9 mg/ml of the extract, respectively, for 28 days. The parameters investigated in the study include packed cell volume (PCV), red blood cell count (RBC), hemoglobin concentration (HBC), total white blood cell (WBC) count, mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and total and conjutate bilirubin and creatinine. Significant (p < 0.05) differences existed between the experimental groups and the controls with respect to the lymphocyte counts, mean corpuscular volume, ALT, AST, and ALP. No significant difference existed in the levels of PCV, RBC, HBC, WBC count, neutrophil count, MCH, MCHC, and total and conjugated bilirubin and creatinine. Phytochemical analysis of the extract showed the presence of tannins and flavonoids.     

Effects of caffeine extract from kola nut on body weight, hematology, sperm reserve and serum enzyme activities in albino rats

This study investigated the effects of caffeine extract from kola nut on body weight, hematological indices, serum enzyme activities, and testicular morphology and function in male albino rats. Ten rats from a total of 58 rats used for this study were used for the assessment of baseline body weight, hematological indices, serum enzyme, and sperm reserve values. Later, three groups of 12 rats (groups A, B, and C) received 10, 20, and 30 mg/kg body weight dose levels of caffeine extract daily, respectively, via the intraperitoneal route for 30 days. The group D rats (n = 12) served as the control and received no caffeine extract treatment. Administration of caffeine extract from kola nut in rats led to significant (P < 0.05) reduction in mean body weight, packed cell volume, erythrocyte count, mean corpuscular hemoglobin concentration, and hemoglobin concentration. Mean total leukocyte count did not differ significantly (P > 0.05) in the three treatment groups relative to the control. Increasing the dose levels of the caffeine extract (10, 20, and 30 mg/kg body weight) significantly (P < 0.05) elevated the mean aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase values. Exposing rats to graded dose levels of caffeine extract led to a significant (P < 0.05) reduction in both gonadal and extra-gonadal sperm reserves. Liver sections of rats that received 30 mg/kg body weight of caffeine extract revealed enlarged portal ducts with cellular proliferation around the portal duct. The results of this study have not only shown the adverse effects of caffeine extract from kola nut in rats but have also provided additional knowledge and information to the existing pathophysiological implication of caffeine intake.     

Acute and chronic toxicity studies of Erythrina senegalensis DC stem bark extract in mice

The toxicity of chloroform stem bark extract of Erythrina senegalensis DC, a medicinal plant with anti-inflammatory activity, was studied in vivo and in vitro. The LD₅₀ i.p. of the extract was 526 mg/kg after an acute toxicity test (24 h). A brine shrimp lethality test with the extract gave LC₅₀ of 60.86 ppm. The chronic studies revealed alterations in the levels of biochemical markers of hepatic and cardiac damage. The alterations were, however, not significant except in the group fed the highest inclusion of the extract (1.0 g extract/kg feed) where significant (p < 0.05) increases in the activities of alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase were observed. Hematological assessments of mice in this group showed significant (p < 0.05) decreases in the red blood cell count and the packed cell volume. Increases in the relative weights of the liver and heart were also significant (p < 0.05). Lipid peroxidation product levels assayed as malondialdehyde was significantly (p < 0.05) elevated in the groups fed 0.5 and 1.0 g/kg of feed at day 84. Significant histopathological changes like myocardial hemorrhages and degeneration of hepatocytes were observed in the heart and liver respectively.     

Investigation into the short-term effect of ingestion of different concentrations of saccharin sodium on some hematological and biochemical profile of rats

Saccharin is a crystalline, non-caloric artificial sweetener that is normally added in foods and pharmaceutical products. Its use as an artificial sweetener has generated so much controversy with respect to toxicity. This work investigated the short-term effects of this sweetener on some hematological and biochemical profile of rats. The rats weighing between 150–180 g were divided into groups A, B, C, and D of five animals each. Group A received water and served as the control. Groups B, C, and D received per os 10%, 15%, and 20% of saccharin in water. The treatment lasted for 28 days. Blood samples were collected for hematological and biochemical analyses before dissection of internal organs. The relative weights of liver, spleen, lungs, heart, and kidney were determined. No significant difference was found in the mean levels of the packed cell volume, red blood cell count, differential leukocyte counts, and hemoglobin concentration in comparison with the controls that received equal volume of distilled water. Significant (p < 0.05) decrease was found in the level of total white blood cell count in the group that received 20% saccharin in comparison with the control. The decrease is still within the normal range found in rats. There was also no significant difference between the activities of the serum enzymes, aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase. The mean relative weights did not change significantly in all the organs studied. The level of creatinine did not also differ significantly. Based on the findings in this work, exposure to saccharin may not pose any serious risk to humans.     

Measurement of hematological and biochemical studies on wild marine carnivorous fishes from Vellar estuary, southeast coast of India

Hematological parameters have been recognized as valuable tools for monitoring fish health. Hematological and serum biochemical parameters were studied and compared between four marine carnivorous fish. Statistical analysis revealed that differences in hematological parameters between marine carnivorous fish were significant (P < 0.01). The result revealed that hematological red blood cells (RBC)/white blood cells (WBC) ratio, mean corpuscular volume, and mean cell hemoglobin concentration were significantly correlated at the P < 0.05 level. The RBC/WBC level increased due to the decrease in WBC during the study. Serum biochemical parameters can be used for confirming the maturity and monitoring any changes in the quality of waters and related soils.     

Effects of drinking diesel-contaminated water on the hematological and serum biochemical parameters of mice

Pollution of the environment by petroleum products is a common feature in oil producing nations, especially in the developing countries where the incidence of pipe line leakage and valdalization is very common. This study investigated the effects of exposure to water contaminated with very low concentration of diesel on some hematological and serum biochemical parameters of mice. A total of 20 albino mice were used for the study. The mice were allowed to drink from water contaminated with diesel 1 %, v/v for 6 weeks. Hematological and serum biochemical parameters of the mice that were exposed to the contaminated water were compared to control mice. There was a significant (p<0.05) decrease in the packed cell volume, hemoglobin concentration, red blood cell count, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration of mice that were allowed to drink from the diesel-contaminated water. The mean corpuscular volume also increased significantly (p<0.05) in these mice. The activities of these serum enzymes; aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase increased significantly (p<0.05). Serum concentrations of total bilirubin and creatinine also increased significantly (p<0.05). The results indicate that diesel-contaminated water or products even at the lowest concentration is a threat to the environment. The health of humans and other species exposed to this environmental pollutant is adversely affected.     

Measurement of some haematological characteristics of the wild carp

The aim of the present study was to obtain a basic knowledge of the haematology and the influence of sex on some blood parameters of wild carp (Cyprinus carpio) spawners. Haematological indices [red blood cells (RBC), white blood cells, haemoglobin (Hb), packed cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) and leucocyte differential count] were measured in one blood sample from 24 females (weight, 1.3 ± 0.1 kg; length, 47.4 ± 1.06 cm) and 27 males (weight, 1.265 ± 0.105 kg; length, 46.9 ± 0.8 cm). The highest haematocrit (PCV), haemoglobin concentration (Hb), RBC, MCH and MCHC were found for males. The highest leucocyte differential were also found for females. Statistical analysis revealed that differences in haematological parameters between males and females fish were not significant.     

Evaluation of haematological and serum biochemical parameters in Iranian camels (Camelus dromedarius) infected with haemotrophic Mycoplasma (Eperythrozoon) spp

Blood samples were collected from 67 adult Iranian dromedary camels naturally infected with Mycoplasma spp, and a control group comprised 20 healthy dromedary camels. Haematological and serum biochemical parameters were measured using standard techniques. In Giemsa-stained peripheral blood smears, Mycoplasma appears attached to the surface of erythrocytes. In infected camels, the number of red blood cells, haemoglobin concentration and haematocrit (packed cell volume) significantly decreased (P < 0.05).With regard to the values of mean corpuscular volume and mean corpuscular hemoglobin concentration, a normocytic and normochromic anaemia was observed in infected camels. In infected camels, the concentration of serum glucose was significantly lower as compared with controls (P < 0.05).     

Elevated mean corpuscular volume in patients with hereditary hemochromatosis

Previous studies have reported that patients with hereditary hemochromatosis have higher red cell volumes than normal controls. We studied the relationship of mean corpuscular volume (MCV) at diagnosis versus serum transferrin saturation (Tfsat), serum ferritin level (SF), phlebotomy-mobilizable iron (Mob Fe) stores, and HFE genotype in our patients. The study population included 227 patients. One hundred forty-two of 227 were homozygous for C282Y; the mean MCV of this group was 94.9 fL (control group, 89.2 fL; p < 0.001). Among all patients with Mob Fe greater than 4 g, the mean MCV was 94.9 fL (significantly greater than control, p < 0.001). Among C282Y homozygous individuals, there was no significant correlation between the MCV versus Tfsat, SF, and Mob Fe (r = 0.07, 0.207, and −0.005, respectively). In patients with Mob Fe greater than 4 g who were not C282Y homozygous, there was weak correlation between the MCV versus Tfsat and SF (r = 0.35 and 0.31, respectively) and no significant correlation versus Mob Fe (r = 0.06). Our study confirms the presence of elevated MCV levels in C282Y homozygous individuals as well as those with elevated Mob Fe. For the most part, the elevated MCV did not correlate with measures of body iron stores.     

Effects of different dietary prebiotic inulin levels on blood serum enzymes, hematologic, and biochemical parameters of great sturgeon (Huso huso) juveniles

An 8-week growth trial was carried out in freshwater fiberglass tanks to investigate the effects of different dietary prebiotic inulin levels on hematologic and biochemical parameters and some blood serum enzymes in juvenile great sturgeon (Huso huso). Four experimental diets were formulated to contain different prebiotic inulin levels (0.0%, 1.0%, 2.0%, and 3.0%, respectively). Triplicate groups of fish (50 fish per tank with an average body weight of 16.14 ± 0.38 g) were assigned to each diet. Blood samples were taken from the caudal vein of 60 apparently healthy fish at the end of the trial. No significant differences were observed in serum enzymes between the treatment groups (P > 0.05). The results show that with the increase in supplementation level of inulin, the mean values of alkaline phosphatase decreased but the mean value of other enzymes increased. white blood cell count increased significantly in group treated with 1% inulin compared with the other groups (P < 0.05). No significant difference between the treatment groups was observed in some hematologic and biochemical parameters such as red blood cell count, mean corpuscular hemoglobin (MCH), and glucose (P > 0.05). With the increase supplementation level of inulin, the mean values of cholesterol, MCH, and MCH concentration increased, but the mean value of glucose, white blood cell count, red blood cell count, hematocrit, hemoglobin, and mean corpuscular volume decreased.     

Change in haematological profile of pregnant camels (Camelus dromedarius) at term

Haematological parameters of 28 pregnant camels (Camelus dromedarius) were compared with those of 32 non-pregnant camels (C. dromedarius). The parameters compared were: total erythrocytes count (RBC), haemoglobin (Hb), packed cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration and total leucocyte count (TLC). Results obtained indicate that RBC, Hb and PCV decreased in the later stages of pregnancy while TLC remained unchanged. Calculated indices revealed a significant increase in MCV (p < 0.02) of pregnant camels.     

Hepatoprotective Effect of Infliximab,an Anti-TNF-α Agent,on Carbon Tetrachloride-Induced Hepatic Fibrosis

To assess the effect of infliximab, an anti-tumor necrosis factor (TNF)-α agent, on the carbon tetrachloride (CCl₄)-induced hepatic fibrosis in rats. Rats were randomized into three groups (n = 9). The control group received only intraperitoneal (i.p.) olive oil. Hepatic fibrosis was induced by repeated i.p. injections of 1.5 ml/kg CCl₄ (1:3 mixture with olive oil) for 5 weeks in the remaining two groups which were also injected subcutaneous saline or 2 mg/kg infliximab. Infliximab reduced the levels of aspartate aminotransferase and alanine aminotransferase (p < 0.05 for both). The scores of hepatic necrosis, inflammation and fibrosis, and expression of α-smooth muscle actin were lower in the infliximab-treated group than the CCI₄-treated group (p < 0.01, p < 0.001, p < 0.01, p < 0.001, respectively). However, there was no significant difference in terms of liver tissue and plasma malondialdehyde, and serum TNF-α levels, while infliximab relatively reduced the level of transforming growth factor-β₁ (373.0 ± 153.1 vs. 280.8 ± 127.1 pg/ml). Treatment with infliximab attenuated the necro-inflammation and fibrogenesis in the CCI₄-induced hepatic fibrosis, and thus it might be effective as a therapeutic anti-fibrotic agent.     

Study on acute phase proteins (haptoglobin,serum amyloid A,fibrinogen, and ceruloplasmin) changes and their diagnostic values in bovine tropical theileriosis

This study was conducted to assess the pattern of changes and the relative value of acute phase proteins (haptoglobin (Hp), serum amyloid A (SAA), ceruloplasmin and fibrinogen) in bovine tropical theileriosis. The diseased group comprised 50 dairy Holstein cattle, 2−3 years old, naturally infected with Theileria annulata. Infected animals were divided into four subgroups with different parasitemia rates (<1%, 1−3%, 3−5%, >5%). As a control group, 20 uninfected cattle were also sampled. Blood samples were collected and all measurements were done using validated methods. There were significant differences in red blood cells (RBCs), packed cell volume (PCV), hemoglobin (Hb) and concentrations of Hp, SAA, ceruloplasmin and fibrinogen between healthy cattle and those infected with T. annulata with different parasitemia rates (P < 0.05). As the parasitemia rate increased in infected cattle, a significant decrease was observed in RBCs, PCV, and Hb. In contrast, with increase in the parasitemia rate, a significant increase in the mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), Hp, SAA, ceruloplasmin, and fibrinogen was evident. The optimal cut-off point was set by the receiver operating characteristics (ROC) method to >5.68 μg/ml for SAA, >0.16 g/l for Hp, >0.064 g/l for ceruloplasmin and >4.00 g/l for fibrinogen with corresponding 84% sensitivity and 100% specificity for SAA, 76% sensitivity and 100% specificity for Hp, 84% sensitivity and 80% specificity for ceruloplasmin and 40% sensitivity and 100% specificity for fibrinogen. SAA had the highest sensitivity and specificity and was diagnosed to be a suitable indicator of APPs changes in bovine theileriosis.     

The acute and subchronic toxicity studies of aqueous leaf and stem bark extract of Costus afer ker (Zingiberaceae) in mice

This work investigated the acute and subchronic toxicity profile of aqeous stem bark and leaf extract of Costus afer ker (Zingiberaceae). This plant is used extensively for food, economic, and medicinal purposes in Nigeria. Acute oral toxicity studies in male and female mice did not produce any sign of acute toxicity or death even at the dose of 4,000 mg/kg. Thirty-six albnino Wistar mice of varied sexes weighing between 28 and 35 g were divided into four groups. Groups A, B, and C were given different concentrations of the extract at 20, 13.33, and 6.67 mg/ml, respectively in water. Group D served as the control and was given only water. The experiment lasted for a period of 56 days. The packed cell volume, hemoglobin concentration, and the total red blood cell count decreased significantly (p < 0.05) in comparison with the control. Serum biochemistry showed no significant changes in the activities of alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase when compared with the control. The histopathological studies revealed no significant lesions in the heart, kidney, and liver. There was also no significant chages in weight between the experimental animals and the control.     

Clinicopathologic findings in goats exposed to drinking water experimentally contaminated with varied low percentages of NPK 15�C15�C15 fertilizer

This study investigated the clinicopathology of goats exposed to drinking water experimentally contaminated with varied low percentages of NPK 15–15–15 fertilizer. Twenty West African dwarf (WAD) goats of between 4 and 8 months of age were used for the study. They were randomly divided into four groups (A, B, C, and D) and were given drinking water contaminated with NPK 15–15–15 fertilizer at the level of 3%, 1%, 0.2%, and 0% (uncontaminated control), respectively, as the only source of drinking water for the 12-week period of the study. Data on the hematology, serum biochemistry profile, body weight, and water consumption of the WAD goats were collected at the beginning of the study and also at specific intervals during the study period. At the end of the study period, the goats were humanely sacrificed, and the internal organs were examined grossly and sections of the liver, kidney, heart muscle, lung, and spleen were prepared and examined for histopathological lesions using a light microscope. Results showed that, from the fourth week to the end of the study, the groups given water contaminated with 3% and 1% fertilizer had a significantly lower (p < 0.05) packed cell volume (PCV), red blood cell (RBC) count, and hemoglobin concentration (HbC) when compared to the controls. The platelet counts of the groups given water contaminated with NPK 15–15–15 fertilizer were significantly (p < 0.05) higher than that of the controls at week 12 only. The serum cholesterol levels of the goats given water contaminated with 3% fertilizer was significantly (p < 0.05) higher than that of the controls on weeks 4 and 8 of the study. There was significant (p < 0.05) weight loss and reduction in daily water consumption in all goat groups given contaminated water and significant (p < 0.05) weight gain in the control group. Administration of the fertilizer-contaminated waters did not lead to any significant effects on the mean corpuscular values, total leukocyte count, absolute neutrophil and lymphocyte counts, and all the clinical biochemistry parameters (except serum cholesterol). There were also no significant differences (p > 0.05) in the organ weight percentages of all the groups, and no lesions were observed on the examined tissue sections of the goats in all the groups. It was concluded that contamination of drinking water of WAD goats with NPK 15–15–15 fertilizer as used in this study led to significant reduction in PCV, HbC, RBC counts, elevated serum cholesterol, and weight loss.     

Toxicity of Moringa oleifera seed extract on some hematological and biochemical profiles in a freshwater fish,Cyprinus carpio

The study was carried out to investigate the acute and sublethal toxicity of Moringa oleifera seed extract on hematological and biochemical variables of a freshwater fish Cyprinus carpio under laboratory conditions. The 96 h LC50 value of M. oleifera seed extract to the fish C. carpio was estimated by probit analysis method and was found to be 124.0 mg/L (with 95% confidence limits). For sublethal studies a non lethal dose of 1/10th of 96 h LC50 value (12.40 mg/L) was taken. During acute treatment (96 h), hematological variables like red blood cell count (RBC), hemoglobin (Hb), hematocrit (Hct), and mean corpuscular hemoglobin concentration (MCHC) were significantly (P < 0.05) decreased in fish exposed to seed extract. However a significant (P < 0.05) increase in white blood cell count (WBC), mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH) value was observed in the exposed fish during above treatment period when compared to that of the control groups. Biochemical parameters such as plasma protein and glucose levels significantly declined in fish exposed to seed extract while a significant (P < 0.05) increase in aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) activity was observed. During sublethal treatment (12.40 mg/L), WBC count, MCV, MCH, plasma glucose, AST, ALT and ALP activities were gradually elevated (P < 0.05) at the end of 7, 14, 21, 28 and 35th days in seed extract exposed fish, whereas plasma protein level declined. However, a biphasic trend was noticed in Hb, Hct, RBC and MCHC levels. This study may provide baseline information about the toxicity of M. oleifera seed extract to C. carpio and to establish safer limit in water purification.     

Haematology of the German Shepherd dog in a humid tropical environment

To study the haematology of the German Shepherd breed of dog in a humid, tropical environment, an experiment was conducted to elucidate the effects of sex, age, and breed on the erythrocyte and leukocyte values of this species. There were no significant (p > 0.05) gender-related differences in the values of red blood cell counts (RBC), packed cell volume (PCV), haemoglobin (Hb) concentration, mean corpuscular haemoglobin concentration (MCHC), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), total and differential white blood cell counts (WBC) of the German Shepherd dog. The study also revealed that the adult German Shepherd dog (between 3 and 5 years old) had significantly higher RBC, total WBC, PCV and Hb, MCV, MCH, MCHC and lymphocyte values than a younger animal (between 8 and 15 weeks old). However, neutrophil, monocyte and eosinophil counts were similar (p > 0.05) in the two age groups. Although there were no breed differences in the erythrocyte values between the Nigerian local dog and the German Shepherd dog, the WBC and neutrophil counts were however significantly higher in the Nigerian dog. This study revealed that although there were no gender or breed differences in the erythrocyte values of the German Shepherd dog, there were some age-related differences.     

Effect of cadmium on thyroid function in sheep

The objective of this experiment was to determine the effect of long-term low-dose administration of cadmium on thyroid function in sheep. In this experiment, ten healthy Iranian male sheep, aged about 1 year old, were randomly allocated into two equal groups of control (n = 5) and experiment (n = 5). Both groups were kept under the same conditions of food and environment. Treatment group received cadmium chloride (1 mg/kg/day) orally for 8 weeks. Blood sampling of two groups was done on days 0, 14, 28, 42, 56, and 70 every morning (8 to 9 a.m.). The function of the thyroid was evaluated by measuring the levels of serum thyroid hormones T3, T4, FT3, FT4, and TSH. The hepatic function in both groups was evaluated by measuring hepatic enzyme activities including alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ glutamyl transferase (GGT), and total bilirubin (TBIL). Serum levels of T3, T4, FT3, FT4, and TSH significantly decreased in cadmium-treated sheep when compared to control group (p < 0.05). Serum enzyme activities of ALT, AST, GGT, and TBIL in Cd-treated sheep were significantly increased in comparison with control group (p < 0.05). Our findings suggest that Cd-exposed sheep can be at a risk of low thyroid function.     

Effect of manganese on thyroid function in sheep

Ten healthy adult male sheep aged about 1 year old were randomly allocated into two equal groups of control (no treatment group, n = 5) and experiment (n = 5). The two groups were kept under the same conditions of food and environment. Sheep of experimental group received MnSO₄, H₂O (5 mg/kg/day, SC) from day 0 for 8 weeks. Blood sampling of the two groups were done on days 0, 14, 28, 42, 56, and 70 at 11 a.m. Serum T4, T3, FT3, FT4, TSH, manganese, creatinine, and blood urea nitrogen concentrations were measured. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), and serum alkaline phosphatase (ALP) activities were estimated by conventional methods. Serum and urine GGT activities were also measured. Urine samples were tested by urine dipstick analysis. Results indicated that serum manganese concentration was increased significantly on days 14, 28, 42, 56, and 70 of manganese administration (p < 0.05). The changes in serum enzyme activities ALP, GGT, AST, ALT, and urea and creatinine concentrations during these days were not significant. Changes of urine GGT activity were not significant. Serum TSH, FT3, FT4, T3, and T4 concentrations decreased differently on days 14, 28, 42, and 56 (p < 0.05). Urinalysis by urine dipstick analysis was normal.     

Reference values for the erythrocytic profile of Anak 2000 broilers and Lohmann brown pullets in Nsukka,Eastern Nigeria

Reference values for the erythrocytic indices are vital for the diagnosis of anaemia and polycythemia and also for the assessment of efficacy of therapy instituted to correct these abnormalities. This study determined the reference values for the packed cell volume (PCV), haemoglobin concentration (HbC), red blood cell (RBC) counts, mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) of Anak 2000 broilers (A2B) at 14-day intervals for 84 days and Lohmann brown pullets (LBP) at 21-day intervals for 147 days. A total of 120 chickens were used for the study (60 A2B and 60 LBP), and all haematological determinations followed standard procedures. Results of the determinations on the A2B showed that PCV and HbC did not significantly vary (p > 0.05) with age and had a low correlation with age (r = −0.22 and r = 0.15, respectively), but RBC counts increased with and positively correlated with age (r = 0.78), while MCV and MCH decreased with and were strongly inversely correlated with age (r = −0.80 and r = −0.84, respectively), and MCHC did not follow a definite pattern. For the LBP, there were no significant variations (p > 0.05) associated with age in the PCV, RBC counts, MCV, MCH and MCHC up to 105 days of age; the significant variations (p < 0.05) obtained after day 105 were associated with pre-lay and egg-laying. The HbC of the LBP did not significantly vary (p > 0.05) all through the study. When compared with findings in similar studies as reported by other investigators, some of the values obtained in this study and the patterns of change in relation to age were in agreement while some others contrasted, probably because of differences between the strains and the climatic/environmental conditions under which the chickens were raised.