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1.
Shanila Latif Fahim Zaman Ravindra Veeramachaneni Lamar Jones Norma Uribe-Uribe Elba A. Turbat-Herrera 《Ultrastructural pathology》2013,37(3):199-207
Reactivation of BK polyomavirus (BKV) is increasingly recognized as a cause of failure of renal allografts. Since no specific treatment is available for this infection, early diagnosis is important, as it allows for early intervention and possible recovery of renal function. Forty-four consecutive renal transplant biopsies performed over a 2-year period were included in the study. In addition to evaluation of renal biopsy tissue sections using routine histochemical stains, CD3, CD20, BK virus immunostains using the specific BK virus and the SV40 antibodies and electron microscopy studies were performed. None of the transplant cases but one exhibited classical histologic viral changes. Viral particles were seen by EM in 19%, and BK-virus positivity was identified in only 43% of these cases. CD20-rich inflammatory infiltrates predominated in cases in which either positive BK stain and/or viral particles were identified ultrastructurally. A combined approach using electron microscopic and immunohistochemical evaluation can be utilized effectively to identify BK virus-associated nephropathy at an early phase facilitating early clinical intervention. 相似文献
2.
Hermonat P L Han L Wendel P J Quirk J G Stern S Lowery C L Rechtin T M 《Virus genes》1997,14(1):13-17
In this study the possible role of human papillomaviruses (HPV) in spontaneous abortions is addressed by assaying for HPV DNA in first trimester spontaneous and electively aborted products of conception materials enriched for chorionic villi. The presence of HPVs was measured by polymerase chain reacton (PCR) amplification and DNA dot blot hybridization using an internal probe. The broad spectrum HPV primers were directed to amplify E6/E7 junction sequences, while the probe was of an HPV-16 sequence with significant homology to HPV-6/11. The quantity and quality of isolated DNA was also analyzed and compared by observing the PCR amplification of a cellular sequence from the human -globin gene. Fifteen of the 25 spontaneous samples (60%) were found to be positive for HPV E6/E7 sequences. In comparison, only 3 of the 15 elective samples (20%) were positive. This is the first study of HPV in fetal materials to incorporate material from elective abortions as a control group. Although confounding contamination from the cervix and vagina cant be ruled out, these data are significant and strongly suggest that HPVs are elevated in spontaneously aborted products of conception. Furthermore, these results suggest the possibility that HPVs may be etiologic agents of at least some spontaneous abortions. 相似文献
3.
用聚合酶链反庆法检测宫颈癌中人乳头瘤病毒16型转化基因E7,检出阳性率为10/22,其中4例进行PCR-SSCP分析发现该基因与标准对照株存在明显变异。核苷酸序列分析证实有两处发生碱基突变。研究提示,PCR方法能快速检测宫颈癌中人乳头瘤 转化基因,可作为宫颈癌的辅助诊断方法,SSCP分析能发现病毒株间小变异的基因片段。 相似文献
4.
Human papillomavirus infection and invasive cervical neoplasia: a study of prevalence and morphology
The prevalence of human papillomavirus (HPV) DNA sequences in 45 cervical cancer biopsies was examined with the hot-start polymerase chain reaction (PCR), employing HPV consensus primers from the L1 region. The cases comprised 38 squamous cell carcinomas, three adenosquamous carcinomas, and four adenocarcinomas. PCR products were typed with single-strand conformation polymorphism (SSCP) and the HPV types detected were correlated with tumour type. Forty-three biopsies were HPV-positive, HPV16 being the most prevalent type. HPV18/33/45/58 were also detected, but no low-risk or multiple types. Keratinizing squamous cell carcinoma was invariably associated with HPV16 and adenosquamous carcinoma and adenocarcinoma with HPVs 18/45. Non-keratinizing squamous cell carcinomas harboured all five detected types. Our data corroborate the view that malignant cervical tumours are almost invariably associated with high-risk HPV and that certain malignant cervical tumour phenotypes correlate with specific HPV types. © 1997 John Wiley & Sons, Ltd. 相似文献
5.
目的 调查湖北省女性人群人乳头状瘤病毒(human papillomavirus,HPV)感染状况,为宫颈癌防治提供参考依据.方法 收集2013年1月至2014年11月在湖北省中山医院就诊的3956例宫颈脱落细胞标本,采用PCR体外扩增和DNA反向点杂交相结合的DNA芯片技术,对宫颈脱落细胞标本进行HPV基因分型检测.结果 3956例患者中共检出HPV阳性818例,阳性率为20.68%,其中高危型HPV感染阳性率为15.77%(624/3956).在被检测的23个HPV-DNA亚型中,最常见的依次为16型(3.72%,147/3956),52型(3.67%,145/3956),43型(3.64%,144/3956),58型(2.78%,110/3956),18型(1.49%,59/3956),33型(1.06%,42/3956),未检出MM4型.HPV阳性者中多重感染率为17.36%(142/818),以二重感染最常见(85.21%,121/142).结论 湖北省地区高危型HPV感染以HPV16、52、58、18和33比较常见,低危型HPV感染以HPV43比较常见. 相似文献
6.
慢性宫颈炎患者宫颈中人乳头瘤病毒基因的检测 总被引:6,自引:0,他引:6
应用聚合酶链反应技术检测正常妇女与慢性宫颈炎患者宫颈人乳头瘤病毒感染情况的结果显示,南京市慢性宫颈炎患者HPV-DNA总阳性率达58.6%;HPV6,11,16,18型阳性率分别为29.3%,30.7%,28.6%和34.3%,而正常妇女中HPV总阳性率为19.4%,4个型别HPV阳性率为9.7%,16.1%,3.2%和3.2%,明显低于宫颈炎患者。如皋市慢性宫颈炎患者HPV-DNA总阳性率高达8 相似文献
7.
8.
Haruka Nakada Koichiro Yuji Masaharu Tsubokura Yukio Ohsawa Masahiro Kami 《Journal of medical Internet research》2014,16(5)
Background
A national agreement on human papillomavirus (HPV) vaccination was achieved relatively quickly in Japan as compared to the United States and India.Objective
The objective was to identify the role of print and online media references, including references to celebrities or other informants, as factors potentially responsible for the relatively rapid national acceptance of HPV vaccination in Japan.Methods
A method of text mining was performed to select keywords, representing the context of the target documents, from articles relevant to the promotion of HPV vaccination appearing in major Japanese newspapers and Web pages between January 2009 and July 2010. The selected keywords were classified as positive, negative, or neutral, and the transition of the frequency of their appearance was analyzed.Results
The number of positive and neutral keywords appearing in newspaper articles increased sharply in early 2010 while the number of negative keywords remained low. The numbers of positive, neutral, and negative keywords appearing in Web pages increased gradually and did not significantly differ by category. Neutral keywords, such as “vaccine” and “prevention,” appeared more frequently in newspaper articles, whereas negative keywords, such as “infertility” and “side effect,” appeared more frequently in Web pages. The extraction of the positive keyword “signature campaign” suggests that vaccine beneficiaries cooperated with providers in promoting HPV vaccination.Conclusions
The rapid development of a national agreement regarding HPV vaccination in Japan may be primarily attributed to the advocacy of vaccine beneficiaries, supported by advocacy by celebrities and positive reporting by print and online media. 相似文献9.
A new type-specific, sensitive, non-radioactive assay is described for the detection of human papillomavirus (HPV) DNA in tissues. Sequences within the E6 gene were amplified by the polymerase chain reaction (PCR), using primer pairs which clearly distinguish HPV types, including those with close sequence homology such as 6b and 11. The amplified DNA products were identified by non-radioactive oligonucleotide hybridization and restriction endonuclease mapping, and the method was sufficiently sensitive to detect between 3 and 5 SiHa cells (each containing 1-2 copies of HPV 16 DNA) amongst 10,000 non-HPV-containing cells. Frozen and archival paraffin sections were equally acceptable substrates for the reaction. The assay was applied to frozen sections of full thickness cervical epithelium from 60 cases of cervical intraepithelial neoplasia (CIN) and 24 normal cervical controls. HPV DNA was detected in 60 per cent of cases of CIN 3 and CIN 2, in 25 per cent of cases of CIN 1, and in none of the normal controls. Prevalence of HPV 16 was similar (approximately 50 per cent) in both CIN 2 and CIN 3, and in the whole series HPV 16 was almost five-fold more common than HPV 18. Low-risk HPV types were present in 5 per cent of CIN 1, but 0 per cent of CIN 2 and CIN 3 biopsies. The data emphasize the biological similarity of CIN 2 and CIN 3 lesions, and their divergence from CIN 1. 相似文献
10.
用通用引物聚合酶链反应对宫颈刮片中人乳头瘤病毒感染的快速筛检 总被引:1,自引:0,他引:1
用通用引物聚合酶链反应(GP-PCR)可同时对人乳头瘤病毒(HPV)6、11、16、18、31、33等型进行检测。该方法成功地用于临床宫颈刮片样品广谱HPV感染的筛检。结果,宫颈癌组HPV检出率(85.5%)明显高于非癌组(13.9%)(P<0.01);对宫颈癌放疗前后HPV阳性率比较,二者亦有明显差异(85.5%,43.7%,P<0.01)。证明GP-PCR是一种在大规模人群中快速筛检多型HPV感染的有效方法。 相似文献
11.
《Indian journal of medical microbiology》2019,37(2):241-247
Background: Many human papillomavirus (HPV) types are associated with cervical cancer (CC). Therefore, HPV genotyping has both clinical and epidemiological importance. HPV 16 and 18 are two principal high-risk types responsible for more than 70% of all CC cases. Although several commercial and non-commercial genotyping assays are available, there is a need for a cost-effective and sensitive genotyping method for low- and middle-income countries. Methods: The study was aimed at evaluation of loop-mediated isothermal amplification (LAMP) assay for HPV genotyping in cervical samples. A total of six primer sets for each HPV type were selected for the assay. The LAMP assay was standardised and validated with HPV control panel. Cervical biopsies were subjected to nested multiplex polymerase chain reaction (NM-PCR; as a part of routine diagnostic workup) and LAMP (HPV 16 and 18) simultaneously. Results: A total of 225 clinical samples were processed during the study period. The sensitivity of the assay was determined using the 10-fold dilutions of positive controls. Both the HPV 16-LAMP and HPV 18-LAMP assays were shown to detect as low as 10 viral copies per reaction, which is similar to that of NM-PCR. The LAMP assay had a good agreement (new cases; 92%, post-chemoradiotherapy [post-CRT]; 89.1%) with NM-PCR for the detection of both HPV 16 and 18. As compared to histology (new cases; 79.8%, post-CRT; 51.3%), LAMP had better agreement with NM-PCR for detection of HPV from post-CRT cases. Conclusions: We evaluated the LAMP assay for simultaneous detection and typing of HPV 16 and 18. The assay had good agreement with NM-PCR for detection of both HPV 16 and 18. The LAMP assay is a promising tool for HPV genotyping along with routine cervical cytology, especially in resource-constrained settings. 相似文献
12.
Human breast cancer cell metastasis to long bone and soft organs of nude mice: a quantitative assay 总被引:3,自引:0,他引:3
V. Sung D. A. Cattell J. M. Bueno A. Murray J. A. Zwiebel A. D. Aaron E.W. Thompson 《Clinical & experimental metastasis》1997,15(2):173-182
Bone is a common metastatic site in human breast cancer (HBC). Since bone metastasis occurs very rarely from current spontaneous or experimental metastasis models of HBC cells in nude mice, an arterial seeding model involving the direct injection of the cells into the left ventricle has been developed to better understand the mechanisms involved in this process. We present here a sensitive polymerase chain reaction (PCR) method to detect and quantitate bone and soft organ metastasis in nude mice which have been intracardially inoculated with Lac Z transduced HBC cells. Amplification of genomically incorporated Lac Z sequences in MDA-MB-231-BAG HBC cells enables us to specifically detect these cells in mouse organs and bones. We have also created a competitive template to use as an internal standard in the PCR reactions, allowing us to better quantitate levels of HBC metastasis. The results of this PCR detection method correlate well with cell culture detection from alternate long bones from the same mice, and are more sensitive than gross Lac Z staining with X-gal or routine histology. Comparable qualitative results were obtained with PCR and culture in a titration experiment in which mice were inoculated with increasing numbers of cells, but PCR is more quantifiable, less time consuming, and less expensive. This assay can be employed to study the molecular and cellular aspects of bone metastasis, and could easily be used in conjunction with RT-PCR-based analyses of gene products which may be involved with HBC metastasis. 相似文献
13.
Many studies have suggested that human papillomavirus (HPV) infection plays an important role in the carcinogenesis of the cervical adenocarcinoma. However, the prevalence of HPV infection in cervical adenocarcinoma and adenosquamous carcinoma varies among the studies. Cervical adenocarcinoma (24 cases) and adenosquamous carcinoma (16 cases), including the underlying non-neoplastic epithelium were examined for HPV-DNA using in situ polymerase chain reaction (PCR), which enabled visualization of the localization on a glass slide. In adenocarcinoma, HPV-DNA was found in 13 cases (54%) and in eight cases in underlying non-neoplastic epithelium, resulting in a total of 21 positive cases (88%). In adenosquamous carcinoma, HPV-DNA was detected in 12 cases (75%) and and the HPV-DNA localization of each component was pure adenocarcinoma, 28.6%; mixed, 54.5%; and pure squamous cell carcinoma, 83.3%. In the underlying non-neoplastic epithelium, HPV-DNA was found more frequently in the squamous epithelium (73.3%) than the cervical glands (6.3%). In conclusion, HPV-DNA was detected in 54% of adenocarcinoma, and the rate was elevated by HPV localization in the underlying non-neoplastic epithelium. HPV infection in the underlying squamous epithelium might be related to the carcinogenesis, even in cervical adenocarcinoma. HPV-DNA localization was different in each component of adenosquamous carcinoma. 相似文献
14.
《Indian journal of medical microbiology》2020,38(1):66-71
Purpose: The purpose of this study was to determine the prevalence of human papillomavirus (HPV) in the population in Kunming, Yunnan, China, before and after the introduction of HPV preventive vaccines. Materials and Methods: In total, 28,959 patients were enrolled in this study between 1 January 2016 and 31 August 2019. HPVs were genotyped using a flow-through hybridisation technique, and differences in HPV infection rates before and after the introduction of an HPV vaccine were determined. Results: The prevalence of HPV before and after the introduction of HPV vaccines was 17.74% and 17.11%, respectively. This difference was not statistically significant (χ2 = 1.920, P > 0.05). The HPV infection rates showed a bimodal U-shaped curve for all age groups. The most common genotypes of HPV detected were HPV52, HPV16, HPV58 and HPV39. Conclusion: Although the overall HPV infection rate in Kunming did not change significantly after the introduction of HPV vaccines, differences in HPV infection rates and multi-typic HPV infection rates were evident in certain age groups. 相似文献
15.
Christine C. Roberts Kai‐Li Liaw Finn Egil Skjeldestad Kathrin U. Jansen Janine T. Bryan 《Journal of medical virology》2009,81(9):1620-1626
HPV testing is a valuable tool in cervical cancer screening and efficacy assessment of HPV vaccines. Concordance of specimens from three sites for detection of HPV DNA in the female genital tract was evaluated. At a single visit, the following specimens were collected: an endo‐ecto‐cervical swab (EEC), labial/vulvar/perineal/perianal swab (LVPP) and cervicovaginal lavage (CVL). Specimens were evaluated with HPV6, HPV11, HPV16, and HPV18 type‐ and gene‐specific PCR assays. Of the 898 women evaluated at baseline, 232 were HPV PCR positive in at least one specimen. Of these, for HPV6, HPV11, HPV16, and HPV18, respectively, throughout: (a) 70.4%, 40.0%, 65.3%, and 64.1% tested three‐site positive; (b) 13.6%, 30.0%, 19.7%, and 18.8% tested two‐site positive; and (c) 16.4%, 30.0%, 15.0%, and 17.2% tested single‐site positive. For patients who tested single‐site positive for HPV6, HPV11, HPV16, or HPV18, respectively, the specimen was: LVPP in 92.3%, 33.3%, 68.2%, and 72.7%; EEC in 0.0%, 33.3%, 18.2%, and 9.1%; and CVL in 7.7%, 33.3%, 13.6%, and 18.2%. Combining results of swab specimens together increases detection of HPV6, HPV11, HPV16, and HPV 18, respectively, to 98.7%, 90.0%, 97.9%, and 96.9%. HPV DNA is detectable from all three sites using type‐specific PCR assays; most women who tested positive for a given HPV type were positive for that type in all three specimens. J. Med. Virol. 81:1620–1626, 2009. © 2009 Wiley‐Liss, Inc. 相似文献
16.
Marta Kicia Maria Wesolowska Katarzyna Jakuszko Zaneta Kopacz Bohumil Sak Dana Květonová Magdalena Krajewska Martin Kvá? 《Journal of clinical microbiology》2014,52(5):1780-1782
A urinary tract coinfection, caused by Encephalitozoon cuniculi genotype II and Enterocytozoon bieneusi genotype D, was identified in an HIV-seronegative renal transplant recipient kept under lifelong immunosuppression. To our knowledge, this is the first report describing concurrent infection with these two microsporidia species in organ transplant recipients. 相似文献
17.
Prevalence of human papillomavirus,Epstein‐Barr virus,and cytomegalovirus in fine needle aspirates from lung carcinoma: A case–control study with review of literature
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Parikshaa Gupta MD DNB Dipanjan Haldar MD Jasmine Naru Msc PhD Pranab Dey MD MIAC FRC Ashutosh Nath Aggarwal MD Ranjana Walker Minz MD DNB Ritu Aggarwal MD DNB 《Diagnostic cytopathology》2016,44(12):987-993
18.
Kandali Samwel Crispin Kahesa Julius Mwaiselage Daniela Gonzalez John T. West Charles Wood Joel Palefsky Peter C. Angeletti 《Journal of medical virology》2019,91(2):308-316
We have tested a multiplex polymerase chain reaction (PCR) human papillomavirus (HPV) genotyping assay to fill the need for rapid and low-cost HPV detection in Sub-Saharan Africa. This method allows high throughput genotyping and simultaneous detection of 14 high-risk and two low-risk HPV types, by PCR amplification of HPV DNAs in a single reaction tube. In this study, we describe stepwise experiments to validate the multiplex HPV PCR assay for determination of HPV genotypes from 104 cervical brush samples from Tanzanian women. Assay performance was evaluated by determination of intra-laboratory reproducibility, sensitivity, and specificity. Further performance was assessed by comparison with the widely accepted and validated HPV My09/My11 amplification and hybridization assay. Statistics; the Cohen kappa (κ) and McNemar P values were used to analyze interobserver and intermethod agreement. Overall concordance between the multiplex and line blot hybridization assays was 99% (per sample) with a κ value equal to 0.95; and 96.49% (per detection event) with a κ value of 0.92. Interobserver reproducibility of the assay per sample was 95.76% with κ of 0.91. These results demonstrate that the multiplex HPV PCR assay has high analytical sensitivity and specificity in detecting as many as 16 different HPV genotypes and that its simplicity and low cost makes it well suited for sub-Saharan Africa. 相似文献
19.
In order to detect human papillomavirus (HPV) DNA in invasive cervical cancers, three different polymerase chain reactions to amplify different subgenomic fragments of HPV DNA were carried out on DNA extracted from 93 formalin fixed and paraffin-embedded tumor tissues. This study detected HPV DNA in 54 cases (58.1%), which broke down to HPV 16 in 39 (41.9%) cases, HPV 18 in six (6.4%), HPV 52 in three, HPV 33 in one and unclassified HPV type in the remainder. Histopathologically, squamous cell carcinomas frequently contained HPV 16, whereas, HPV 18 was present in adenocarcinoma, adenos-quamous cell carcinoma and small cell carcinoma of the cervix. Clinicopathological study revealed that HPV 16 and 18 DNA found were more frequently than other HPV subtypes in premenopausal patients. Moreover, HPV 18 DNA positive cancers had a relatively high recurrence rate. These results indicate that cervical cancers might be clinically influenced by the difference in subtypes of the infecting HPV. Acta Pathol Jpn 42: 876–883, 1992. 相似文献
20.
Chin‐Hung Wang Ronald Garingalao Garvilles Chung‐Yung Chen 《Journal of medical virology》2010,82(8):1416-1423
The detection of human papillomavirus (HPV) is very important for the evaluation of preventative strategies for cervical cancer. The major objective of this study was to characterize the prevalence of different genotypes of HPV in north Taiwan to contribute to the epidemiological knowledge of HPV infections. Papanicolaou (Pap) cervical smears were collected from 10,543 women aged between 14 and 87 years. The polymerase chain reaction (PCR) and DNA array hybridization techniques were used to genotype 51 different HPV strains. HPV was detected in 1,577 women, which gave an overall HPV prevalence rate of 15%. Forty‐eight different genotypes were found in these patients, which included 9.7% that were high‐risk HPV (HR‐HPV) genotypes. The most common types of HR‐HPV in patients, in descending order of frequency, were HPV 52, 16, 58, 56, 39, 51, 18, 68, 31, 33, 59, 45, and 35. HPV 52 was the most frequent type in every age group. The four most common HR‐HPV types were found in 56.6% of the patients infected with HR‐HPV. In cases that were infected with multiple HPV genotypes, 69.2% had at least one HR‐HPV genotype. The rate of infection with HR‐HPV was higher in the younger age groups than the older ones. In conclusion, 48 HPV genotypes were identified from a large study of cervical screening samples and the prevalence of HPV genotypes in different age groups was very diverse. The formulation of a public health strategy for HPV vaccination should take into account the prevalence of various HR‐HPV/LR‐HPV genotypes. J. Med. Virol. 82:1416–1423, 2010. © 2010 Wiley‐Liss, Inc. 相似文献