含透明质酸及白藜芦醇成分护肤品对干性皮肤屏障功能的影响

目的研究含有透明质酸和白藜芦醇的安特柔产品对于干性皮肤屏障功能的影响及保湿效果。方法入选皮肤中度干燥的女性受试者32例,采用自身左右对照的方法,比较安特柔沐浴洗发露与普通洁肤香皂清洁后皮肤屏障功能的差别,遂后使用安特柔保湿霜,观察6 h后及连续试用产品1周后皮肤屏障功能指标的变化。结果相比普通洁肤香皂清洁,安特柔产品清洁后皮肤经皮水分丢失(TEWL)不但没有升高反而下降(P0.05),pH值升高程度低于普通洁肤香皂(P0.05),角质层含水量轻微增加但差异无统计学意义(P=0.480);安特柔保湿霜单次使用后6 h,身体左右两侧皮肤角质层含水量均明显提高、TEWL下降,与基线相比P0.05,皮肤p H值均恢复到基线水平(P=0.094)。连续使用安特柔沐浴洗发露和保湿霜1周后,角质层含水量较基线进一步增加,较单次使用后6 h也有轻度的增加(P0.05);TEWL继续下降,p H值继续维持在偏酸范围内。连续使用1周后干性皮肤者皮肤的光滑度、干燥度和脱屑等均有显著改善;受试者自我评价50%的人认为皮肤的光滑度、干燥度、瘙痒和鳞屑有中度以上改善。结论安特柔系列产品具有很好的保湿效果和帮助屏障功能恢复的作用,单次使用后6 h仍有效,长期使用能很好地改善皮肤干燥。     

两种维生素E乳剂保湿性的比较

目的了解维生素E乳喷雾剂的保湿效果及患者的依从性,与对照乳液比较,评价其优劣。方法共观察40例受试者,采用自身对照试验,分别在基线、2周后、4周后检测靶部位皮肤的角质层含水量、经表皮失水率、pH值,受试者主观评价。结果全部受试者均完成了试验,未发生不良反应。2组之间靶部位的角质层含水量、经表皮失水率、pH值在0,2,4周时差异均无统计学意义（P〉0．05）。2组靶部位的角质层含水量在2周及4周后均较基线时显著升高,差异有统计学意义（P〈0．01）;而表皮失水率和pH值较基线时无明显变化,差异无统计学意义（P〉0．05）。多数受试者（67．5％）认为喷雾剂优于乳膏剂。结论维生素E乳喷雾剂保湿效果好,与对照乳液的保湿效果相当,优点是清爽、无香味。     

空气污染对皮肤角质层蛋白羰基化水平的影响及粉红胡椒木提取物和脂质混合物对皮肤损伤的防护作用

目的 研究空气污染对皮肤角质层蛋白羰基化水平的影响,评估粉红胡椒木提取物和脂质混合物对皮肤损伤的防护作用。方法 预实验分析影响因素后,荧光标记法检测34例健康受试者不同部位皮肤角质层蛋白羰基化水平。利用定制的污染模拟箱,以香烟烟雾模拟污染物, 将15名健康受试者前臂屈侧向上暴露于污染模拟箱中,分别于暴露后0、1、2、4、5 h用D?squame胶片采集角质层样本。选14例健康受试者,在单侧前臂屈侧选择相邻的3个区域分别外用1%粉红胡椒木提取物的水溶液（胡椒木组）、去离子水（对照组）或不涂任何样品（空白组）,然后将手臂在污染模拟箱中暴露5 h,暴露前后采集各区域角质层样本。另选16例健康受试者,在单侧前臂屈侧选取3处区域分别外用含5% 脂质混合物的乳液（脂质混合物组）、不含脂质混合物的安慰剂乳液（对照组）或不涂任何样品（空白组）,然后将手臂在污染模拟箱中暴露5 h,暴露前后采集各区域角质层样本。纳入20例健康受试者进行双盲半脸临床测试,即随机选择半脸外用含1%粉红胡椒木提取物的乳液,另外半脸外用安慰剂乳液,于产品使用前和使用56 d后,采用D?Squame胶片在受试者的面颊部分别采集角质层样本。采用荧光标记法检测上述角质层样本中皮肤蛋白羰基化水平。结果 对34名受试者检测显示,人体不同部位蛋白羰基化水平（平均荧光强度）存在显著差异（P < 0.001）,其中,面颊（26.3 ± 7.1）和额部（22.9 ± 7.9）显著高于手臂（14.7 ± 4.9）和腰背部（12.6 ± 4.2）（均P < 0.001）,且手臂蛋白羰基化水平显著高于腰背部（P = 0.046）。短期模拟加速暴露实验中,蛋白羰基化水平随污染暴露时间的增加而持续升高（R2 = 0.995 9）,暴露5 h后,胡椒木组和脂质混合物组皮肤角质层蛋白羰基化水平升高值分别为9.7 ± 5.2和5.8 ± 4.9,低于各自的空白组（19.0 ± 10.0、17.4 ± 8.8,均P < 0.005）和对照组（18.5 ± 7.3、15.9 ± 6.4,均P < 0.005）,差异均有统计学意义。长期人体测试中,20名受试者使用含1%粉红胡椒木提取物的乳液8周后,与安慰剂侧相比,面部蛋白羰基化水平显著降低。结论 空气污染加剧皮肤角质层蛋白羰基化损伤,粉红胡椒木提取物和脂质混合物能有效降低蛋白羰基化水平。     

三种波长发光二极管光对光老化皮肤的作用研究

【摘要】 目的 探讨并比较530 nm、630 nm以及850 nm发光二极管（LED）光对光老化皮肤的作用。 方法 选择14例皮肤光老化受试者,随机于其一侧前臂伸侧三个区域,分别给予530、630及850 nm LED光照射,每周3次,共12次。对照射部位皮肤进行皮肤镜检查,并比较照射前、照射第2、4和6周皮肤角质层含水量、经皮水分丢失量（TEWL）以及反映皮肤颜色的皮肤亮度（L*值）和皮肤黑素指数（MI值）的变化。同时,随机选择5例、5例、4例受试者在试验前和试验第6周分别取530、630及850 nm LED光照射皮肤进行组织病理检查。 结果 经12次630、850 nm LED光照射后,经皮肤镜检查,分别有10例、7例受试者皮肤纹理变浅,色素沉着减少;7例受试者530 nm LED光照射部位皮肤纹理加深,色素沉着增加。经630和850 nm LED光照射后,14例受试者皮肤角质层含水量均明显升高（6周时分别为37.9 ± 7.7和34.5 ± 7.1 au,照射前分别为33.1 ± 6.1和32.0 ± 7.0 au,照射前后比较,均P < 0.05）,TEWL值均明显降低（6周时分别为9.8 ± 2.5和10.9 ± 2.5 g·m-2·h-1,照射前分别为14.0 ± 1.8和14.2 ± 2.6 g·m-2·h-1,照射前后比较,均P < 0.05）,L*值与MI值均未发现明显改变（P > 0.05）。530 nm组皮肤角质层含水量及TEWL值较照射前差异均无统计学意义（P > 0.05）,L*值显著降低（P < 0.05）,MI值明显增高（P < 0.05）。组织病理检查显示,照射前真皮具有典型的光化性胶原纤维、弹性纤维变性损害,照射后3组均出现新生胶原纤维、弹性纤维,排列较照射前整齐致密。 结论 630和850 nm LED光均可以改善光老化皮肤外观、屏障功能,促进胶原纤维和弹性纤维增生和重排,530 nm LED光会增加皮肤色素沉着。     

含马齿苋及甘草提取物护肤品对敏感性皮肤辅助治疗作用的临床观察

目的:评价含马齿苋及水溶性甘草提取物护肤品(商品名:薇诺娜舒敏保湿修复霜及薇诺娜舒敏控油凝露)辅助治疗敏感性皮肤的有效性及安全性.方法:采用多中心、治疗前后对照的临床试验方法,对110例敏感性皮肤受试者(干、中性敏感性皮肤受试者80例,油性敏感性皮肤受试者30例)面部分别外用薇诺娜舒敏保湿修复霜及薇诺娜舒敏控油凝露,每日2次,连续使用1个月.在治疗前及治疗后7 d、15 d、30 d分别对受试者皮肤进行皮肤生理功能测试,同时对乳酸试验、皮损恢复情况、受试者对该产品的满意度及安全性进行评价.结果:治疗30d后,干性、中性敏感性皮肤组受试者角质层含水量由治疗前71.53%±3.34%增加至74.49%±2.87%,油脂含量由治疗前(74.30±29.19)μg/cm2增加至(90.01±11.45)μg/cm2.油性皮肤组受试者含水量由治疗前71.95%±2.34%增加至75.09%±1.67%,油脂含量由治疗前(247.13±41.90)μg/cm2下降至(223.23±40.19)μg/cm2,治疗前后比较(P<0.001),差异具有统计学意义,而皮肤弹性相比差异无统计学意义;乳酸试验分值由治疗前4.39±0.82降至2.58±0.40(P<0.001),差异具有统计学意义;皮肤改善者87例,占79.09%;与使用前相比(P<0.001),差异具有统计学意义;试验中未出现不良反应.结论:薇诺娜舒敏保湿修复霜及薇诺娜舒敏控油凝露对于、湿性敏感性皮肤有辅助治疗作用,有助于恢复皮肤屏障功能,安全性好.     

青刺果油对神经酰胺合成及神经酰胺酶表达的影响

目的 探讨青刺果油对神经酰胺（Cer）合成及酸性神经酰胺酶（ASH1）表达影响的研究,探讨其部分保湿机制及修复皮肤屏障机制。方法 培养人角质形成细胞,设对照组及实验组,对照组加入不含青刺果油的K-SFM培养液,实验组加入含青刺果油K-SFM培养液,于0、3、8、24、48 h分别取实验组及对照组的上清液,用ELISA方法测定上清液中Cer含量。将裸鼠背部皮肤分为受试区,基质区、空白对照区及阴性对照区,其中前3区用丙酮及乙醚破坏了裸鼠表皮屏障,受试区及基质区分别涂搽含青刺果油乳剂和基质,空白对照区不涂抹任何乳剂,用无创性皮肤测试方法分别于0、1、3、7 d测定裸鼠皮肤经表皮水分流失（TEWL）、表皮含水量及皮脂含量,同时取裸鼠皮肤组织,用免疫组化方法观察ASH1表达。结果 ELISA结果显示,实验组上清液中Cer含量随时间增加而增加,24 h Cer含量（1.3817 ± 0.100）及48 h Cer含量（1.3737 ± 0.047）与0 h（0.7630 ± 0.143）比较,差异有统计学意义（P < 0.05）;实验组与对照组比较,Cer含量高于对照组,24 h、48 h时差异有统计学意义（P < 0.05）。无创性皮肤测试显示,随时间增加,受试区、基质区,空白对照区TEWL值逐渐减少,表皮含水量、皮脂含量逐渐增加,受试区TEWL 3 d（10.85 ± 0.64）、7 d（8.01 ± 0.58）时较0 d（12.65 ± 0.71）低,皮脂含量3 d （29.14 ± 0.40）、7 d（31.30 ± 0.88）时较0 d（27.02 ± 0.65）高,其1 d（13.98 ± 0.28）、3 d（15.00 ± 0.38）、7 d（15.86 ± 0.18）的表皮含水量较0 d（11.74 ± 0.62）高,差异有统计学意义（P < 0.05）。同一时间四区比较,7 d时,受试区的TEWL值较基质区、空白对照区及阴性对照区低,表皮含水量及皮脂含量较基质区、空白对照区及阴性对照区高,差异有统计学意义（P < 0.05）。免疫组化结果显示,受试区、基质区,空白对照区ASH1的表达7 d较0 d高,差异有统计学意义（P < 0.05）;7 d时受试区ASH1的表达较基质区、空白对照区及阴性对照区高,差异有统计学意义（P < 0.05）。结论 青刺果油的保湿及修复皮肤屏障的作用与其增加Cer含量及上调ASH1表达有关。     

季节的变化对广州市健康女性皮肤角质层含水量的影响及校正

目的：探讨季节的变化对广州市健康女性皮肤角质层含水量指标的影响,并建立季节性校正公式。方法：使用皮肤角质层水分测试仪对178例健康女性受试者面颈部皮肤角质层含水量进行四季变化的检测,采用SAS 8.1统计软件进行数据分析,拟合出季节性校正公式,并与环境中紫外线辐射水平和湿度水平进行相关分析。结果：面颊、额部和颈部皮肤角质层含水量在冬季最少（P〈0.05）,除额部皮肤角质层含水量在春季和夏季之间比较无明显差异外（P〉0.05）,其它部位各季节之间比较差异明显（P〈0.05）;各部位皮肤角质层含水量与环境中紫外线辐射水平无相关性（P〉0.05）,与湿度水平呈正相关（P〈0.05）。结论：广州市健康女性皮肤角质层含水量随季节的变化而变化;季节性校正公式可以纠正季节对皮肤角质层含水量的影响;化妆品皮肤保湿功效评估要考虑季节变化对结果的影响。     

薇诺娜舒敏保湿特护霜对新冠疫情防控期间医护人员佩戴医用口罩后皮肤屏障的修复

目的 观察使用薇诺娜舒敏保湿特护霜对疫情期间医护人员佩戴医用口罩后皮肤屏障修复作用及皮肤敏感改善情况.方法 采用自身前后对照研究,选取受试者佩戴口罩后的右侧面颊为受试区.入组当天晨起及佩戴口罩4h后检测经表皮水分流失(transepidermal waterloss,TEWL)及角质层含水量,随后发放受试品(薇诺娜舒敏...     

保湿剂对成人轻度特应性皮炎皮肤角质层神经酰胺含量的影响

目的研究保湿剂对成人轻度特应性皮炎治疗前后皮肤角质层神经酰胺含量及含水量的变化。方法本实验选取25例成人轻度特应性皮炎患者和10例健康对照者进行研究,特应性皮炎患者外用保湿剂治疗;采用胶带剥离法采集实验组和对照组前臂屈侧皮肤角质层样本,用高效液相色谱–质谱法联用定量检测角质层神经酰胺3含量,用SOFT 5.5皮肤性质测试仪测定皮肤含水量;分析治疗前后患者皮肤神经酰胺3含量及含水量的变化,并根据EASI评分法评价疗效。结果实验组皮肤角质层神经酰胺3含量(0.89±0.49)ng/mg、含水量(31.97±12.04)均低于健康对照组[(10.22±2.69)ng/mg和(67.81±11.05)],两组差异有统计学意义(P0.05);实验组治疗4周后皮肤角质层神经酰胺3含量(2.28±0.69)ng/mg和含水量(50.54±17.22)均高于治疗前,差异有统计学意义(P0.05);EASI评分治疗后(0.73±0.66)较治疗前(2.37±1.50)下降,差异有统计学意义(P0.05);皮肤含水量与角质层神经酰胺3含量呈正相关关系(r=0.51,P0.05),与EASI评分呈负相关关系(r=-0.49,P0.05)。结论成人轻度特应性皮炎患者皮肤角质层神经酰胺3含量下降,使用保湿剂辅助治疗能促进患者症状减轻。     

旁氏无暇透白日霜改善黄褐斑及其皮肤屏障功能的疗效观察

目的观察旁氏无暇透白日霜(SPF 15 PA++)改善黄褐斑临床症状及皮肤屏障功能的疗效。方法采用随机双盲、自身对照的临床研究方法。选取30例面部皮损较对称的黄褐斑患者,受试者每天用温水清洁面部,半脸使用约0.5g测试产品,另外半脸使用等量对照产品,早晚各1次,2次/d。分别于首诊、治疗后第28天、56天评估皮损情况。结果①主观比较:实验组基本治愈0例,显效4例,好转10例,无效16例,总有效率46.6%。对照组基本治愈0例,显效1例,好转5例,无效24例,总有效率20.0%。治疗组与对照组疗效经卡方检验差异有统计学意义(P0.05)。②TEWL值:试验组与对照组随时间延长TEWL值均值均有所降低,治疗组与对照组相比0、28、56天P值均0.05,差异无统计学意义。③皮肤角质层含水量:试验组与对照组随时间延长皮肤角质层含水量均值均有所升高,治疗组与对照组相比0、28天P值均0.05,差异无统计学意义,第56天时,试验组角质层含水量(41.14±5.94)与对照组角质层含水量(41.90±5.81)比较,差异有统计学意义(P0.05)。④LAB值检测:随时间延长实验组与对照组LabL值均值均有所升高、LabA值均值无明显变化、LabB值均值均有所下降。实验组与对照组相比,LabL值0、28天P值均0.05,差异无统计学意义;56天时,实验组LabL值(58.92±3.52)与对照组LabL值(56.86±3.11)相比,差异有统计学意义(P0.05)。LabA值0、28、56天P值均0.05,差异无统计学意义。LabB值0、28天P值均0.05,差异无统计学意义;56天时,实验组LabB值(15.21±1.90)与对照组LabB值(16.16±1.52)相比,差异有统计学意义(P0.05)。结论受试品"旁氏无暇透白日霜"和对照品市售"凡士林保湿露"均具有良好的美白、保湿、恢复皮肤屏障的效果,并且"旁氏无暇透白日霜"美白效果显著优于对照品市售"凡士林保湿露"。     

The 24‐hr, 28‐day,and 7‐day post‐moisturizing efficacy of ceramides 1, 3, 6‐II containing moisturizing cream compared with hydrophilic cream on skin dryness and barrier disruption in senile xerosis treatment

Direct replacement of decreased ceramides in the stratum corneum can be efficacious for skin hydration, skin barrier function, and skin pH. Our study aimed to evaluate the 24‐hr, 28‐day, and 7‐day post‐moisturizing efficacy of ceramide‐containing moisturizer in senile xerosis treatment. A split site, double‐blinded, randomized, controlled study was conducted in 24 senile subjects (91.7% females, mean age 54.83 ± 5.45 years) with mild to moderate xerosis, who were randomized to receive ceramide‐containing moisturizer or hydrophilic cream, daily applied on each side of the shin. A single application of ceramide‐containing moisturizer increased skin hydration, while improving transepidermal water loss (TEWL) and skin pH for up to 24 hr, with statistically significant difference. After 28 days of twice‐daily application, more significant improvement on skin hydration, barrier function, and skin pH was observed in those with ceramide‐containing moisturizer at all‐time points. At day 28, there was a statistically significant decrease of hemoglobin index, wrinkle, and texture on the ceramide treated side. The 7‐day post‐moisturizing efficacy on the ceramide treated side was superior for skin hydration, TEWL, skin pH, and wrinkle. Thus, the ceramide‐containing moisturizer can be a novel promising treatment for senile xerosis.     

Cutaneous differences between Black, African or Caribbean Mixed-race and Caucasian women: biometrological approach of the hydrolipidic film

Background/purpose: Studies about the adaptation of ethnic types of skin to an environment radically different from their original environment are rarely found in the literature. We have evaluated the differences in the skin surface properties of three ethnic groups: Black, African or Caribbean Mixed-race and Caucasian women living in defined climatic conditions.
Methods: Biometrological evaluations of barrier function were made by measurements of transepidermal water loss (TEWL), skin hydration and skin dryness. To approach the skin protective coating we evaluated cutaneous lipid index (LI), cutaneous pH, skin frictional properties and skin wettability.
Results: The TEWL, cutaneous hydration, dryness and LI show no significant difference. Volunteers were given a self-assessment questionnaire regarding their hygiene and cosmetic practices. The results show that 100% of Black and 92% of African or Caribbean Mixed-race women use a hydrating product daily vs. only 16% of Caucasian women. They justify this practice by the ' dry skin ' sensation that they feel if they do not use a moisturizer. We tried to assess objectively to the protective coating surface of the human skin: the hydrolipidic cutaneous film, by measurements of pH, frictional properties and wettability which show significant differences. We may suppose that the hydrophilic/lipophilic balance of the skin in these three ethnic groups is different.
Conclusion: To interpret these results as accurately as possible, it would be interesting, in future investigations on the ethnic types of skin, to evaluate the sweat secretion, and to analyse each element of the sebaceous secretions quantitatively as well as qualitatively.     

短波理疗仪治疗红斑毛细血管扩张型玫瑰痤疮临床观察

目的:观察短波理疗仪治疗红斑毛细血管扩张型玫瑰痤疮(ETR)的临床疗效。方法:收集70例ETR患者,随机分为2组,其中治疗组40例,对照组30例。2组均每日早晚使用保湿霜,治疗组每隔1周进行1次短波理疗,疗程8周。治疗前、后由皮肤科医生盲态下对患者面部红斑及毛细血管扩张进行评分;患者对阵发性潮红、灼热及干燥症状进行评分。评分标准采用玫瑰痤疮4级评分。皮肤检测仪检测2组治疗前、后皮肤含水量和经皮水流失(TEWL)值。记录疗程中的不良反应。结果:治疗后治疗组患者面部红斑、灼热及干燥程度评分均较治疗前明显改善(P<0.05),治疗组改善优于对照组(P<0.05);对照组患者灼热及干燥程度评分均较治疗前明显改善(P<0.05)。2组毛细血管扩张评分及阵发性潮红评分比较,差异均无统计学意义(P>0.05)。治疗后2组患者皮肤含水量均增加,治疗组增加大于对照组(P<0.05);2组TEWL值均下降,治疗组下降大于对照组(P<0.05)。所有患者均无明显不良反应。结论:短波理疗仪治疗可明显改善ETR患者面部红斑、灼热及干燥症状,且安全性较好。     

A double‐blind,randomized study to assess the effectiveness of different moisturizers in preventing dermatitis induced by hand washing to simulate healthcare use

Background Healthcare‐associated infection is an important worldwide problem that could be reduced by better hand hygiene practice. However, irritant contact dermatitis of the hands as a result of repeated hand washing is a potential complication that may be preventable by the regular use of an emollient. Objectives To assess the effect of moisturizer application after repeated hand washing (15 times daily) vs. soap alone. Methods In a double‐blind, randomized study, the effect of five different moisturizers on skin barrier function was determined by assessment after repeated hand washing over a 2‐week period in healthy adult volunteers. Assessments of transepidermal water loss (TEWL), epidermal hydration and a visual assessment using the Hand Eczema Severity Index (HECSI) were made at days 0, 7 and 14. Results In total, 132 patients were enrolled into the study. A statistically significant worsening of the clinical condition of the skin as measured by HECSI was seen from baseline to day 14 (P = 0·003) in those subjects repeatedly washing their hands with soap without subsequent application of moisturizer. No change was seen in the groups using moisturizer. Subclinical assessment of epidermal hydration as a measure of skin barrier function showed significant increases from baseline to day 14 after the use of three of the five moisturizing products (P = 0·041, 0·001 and 0·009). Three of the five moisturizers tested led to a statistically significant decrease in TEWL at day 7 of repeated hand washing. This effect was sustained for one moisturizing product at day 14 of hand washing (P = 0·044). Conclusions These results support the view that the regular application of moisturizers to normal skin offers a protective effect against repeated exposure to irritants, with no evidence of a reduction in barrier efficiency allowing the easier permeation of irritant substances into the skin as has been suggested by other studies. Regular use of emollient in the healthcare environment may prevent the development of dermatitis.     

Does massage postapplication improve moisturizer’s efficacy? A 2‐week regression study

Background Combining massage with moisturizer application is a popular technique in beauty spa sessions. The subjective positive psychological effects of massage with moisturizer application in hand and face beauty treatment are documented by many people attending spa sessions. The aim of this study was to objectively evaluate the effect of local effleurage massage as an external intervention on moisturizer efficacy. Methods In a regression‐type study of 2‐week, twice‐daily application followed by 1 week of regression, 13 female subjects applied “off the shelf” moisturizer twice daily on both forearms followed by 1 min superficial massage for one forearm randomized among subjects. The influence of massage after moisturizer application on skin barrier properties was evaluated by noninvasive measurements of transepidermal water loss (TEWL), skin capacitance, and skin elasticity at baseline, day 7, and day 14 during the treatment phase, and day 21 following a 1‐week regression period, in which no moisturizer and no massage were performed on forearms. Results The tested “off the shelf” moisturizer in both “massage” and “no‐massage” application protocols caused a comparable progressive improvement in skin hydration level and barrier permeability over the 2‐week treatment period, which was maintained during the 1‐week regression (no moisturizer) period. In addition, skin elasticity was similarly improved by both application protocols. Conclusion In this long‐term study, the daily performance of massage after moisturizer application was not an effective external intervention for enhancing moisturizer efficacy.     

亚临床皮肤刺激后三种生理参数的变化

目的 探讨经表皮失水(TEWL)、皮肤电容量(CAP)、皮肤表面pH值参数在亚临床刺激性皮炎诊断中应用的可能性.方法 通过1%十二烷基硫酸钠(SLS)开放性斑贴、10次胶带粘贴及0.75最小红斑量UVB照射三种不同的刺激方法,对30名健康女性受试者的前臂屈侧进行处理,共5 d.每天刺激之前进行临床评估.试验第0.6,7,8天进行TEWL、CAP及皮肤表面pH值检测.结果 所有受试者8天内临床评分均为0分;SLS刺激后第6,7,8天TEWL值分别为3.17±3.07(g/m2h)、3.32±2.84(g/m2h)、3.22±2.36(g/m2h),较基础值0.40±1.35(g/m2h)升高,差异均有统计学意义(均为P=0.00).SIS刺激后第6,7,8天CAP值较基础值下降,皮肤表面pH值较基础值升高,与基础值间差异均有统计学意义(P<0.05).胶带粘贴后第6,7,8天TEWL值分别为2.54 ±1.85(g/m2h)、2.40±2.16(g/m2h)、2.17±1.99(g/m2h),较基础值0.11±1.10(g/m2h)升高,差异均有统计学意义(均为P=0.00);胶带粘贴后第8天,皮肤表面pH值较基础值升高,差异有统计学意义(P=0.00);CAP无明显变化.0.75 MED UVB照射后各生物物理参数均无明显变化.结论三种生物物理参数可以被用于亚临床性刺激性接触性皮炎的检测.不同参数对不同的刺激方法检测能力不同.     

Assessment of effects of an additional dietary natural mineral water uptake on skin hydration in healthy subjects by dynamic barrier function measurements and clinic scoring

BACKGROUND/AIMS: The skin protects the body's organs and tissues from damage and physical, chemical and bacteriological injuries. It also prevents the transcutaneous loss of water. The present study was conducted to assess the effects of additional dietary natural mineral water uptake on skin hydration and cutaneous well-being in subjects with dry skin. METHODS: Eighty subjects (44 women and 36 males, mean age 56+/-5.6 years) were included in the study, randomised per forearm and stratified by gender. Skin surface hydration, transepidermal water loss (TEWL), sorption-desorption test, skin colour, thickness and micro-relief were evaluated on the forearms. Clinical scoring of dryness, roughness and elasticity was performed by a dermatologist. RESULTS: An improvement of skin hydration was observed after additional water uptake, statistically modifying the hydration level as well as TEWL, the water-binding capacity of the uppermost layers of the stratum corneum. Improvements of softness, smoothness and skin-moisturising effect were perceived by healthy subjects, and skin micro-relief was improved. CONCLUSION: We suggest that natural mineral water supplementation may be used in order to improve the hydration of skin dryness as a complementary cosmetic approach.     

P16Eyelid dermatitis with positive patch test to coconut diethanolamide

Coconut oil, a traditional moisturizer used for centuries by people in the tropics, does not have any clinical studies documenting its effectivity and safety. This study aims to determine effectivity and safety of coconut oil compared to mineral oil as moisturizer for mild to moderate xerosis. A review board‐approved randomized double‐blind controlled trial was conducted in 34 patients after negative patch‐testing. Patients applied either coconut or mineral oil twice a day for two weeks. Quantitative outcomes for effectivity, measured at baseline and each weekly visit, were skin hydration (Corneometer CM825®) and skin lipids (Sebumeter SM810®); for safety, transepidermal water loss [TEWL](Tewameter TM210®) and skin surface pH (Skin pH meter PH900®). Patients and investigator evaluated symptoms of dryness, scaling, roughness, and pruritus using visual analogue scales (VAS) and grading of xerosis. Both groups showed significant improvement in skin hydration and increased skin surface lipid levels. TEWL and Skin pH were not affected. Objective instrumental determinations showed no significant difference between both groups. Patient and investigator subjective grading of xerosis and VAS showed general trend toward better, though not statistically evident, with coconut over mineral oil. Coconut oil is as effective and safe as mineral oil as a moisturizer.