以银—邻-菲绕啉—镉试剂三元络合物萃取-吸光光度法测定废水中痕量银的研究

本文报导银的新萃取—吸光光度测定法。方法基于在pH9,银与邻-菲绕啉及镉试剂形成组成比为1:2:1的玫瑰红色三元络合物,易被氯仿定量地一次萃取。镉试剂和络合物的最大吸收峰分别位于430nm和525nm处。络合物的mol吸光系数和桑德尔灵敏度分别为5.7×10~4L.mol~(-1).cm~(-1)及0.0019μg/cm~2。络合物的色泽能稳定24h。银浓度介于1～15μg/10ml氯仿具有良好的线性关系。于所试的36种离子中及在Mg-EDTA存在下,仅Pd~(2+)、I~-、CN~-有干扰。本法曾用于废水中痕量银的测定获得满意的结果。     

《中国药典》2015年版羰基值测定方法探讨

目的 :对影响《中国药典》2015年版羰基值测定结果的因素进行分析探讨。方法:对柏子仁及桃仁的羰基值按照三种方法分别进行测定,即药典测定方法,预先蒸馏乙醇、甲苯测定法,按国家标准精制乙醇、甲苯测定法。结果:药典方法测定的结果不稳定,重现性较差,而改进试剂处理方法后的测定结果重现性良好。结论:按国家标准改进后的方法可为药典修改羰基值的测定方法提供参考。     

Pb-TPPS_4-CPB三元络合分光光度法测定水中铅最佳条件的优选

水溶性ＭｅＳｏ -四 - (对碘ＴＰＰＳ4 )和表面活性剂溴化十六烷基吡啶 (简称ＣＰＢ)增溶技术测定水中铅 ,据报道〔1〕与双硫腙法对比试验两法无显著性差异 (Ｐ >0 0 5)。本法选择性好 ,操作简便 ,避免了使用氯仿等有毒试剂 ,不用萃取分离可直接测定痕量铅。实际测定中 ,由于样品溶液中存在着多种共存离子 ,不但对ＴＰＰＳ4 亦有消耗温度及水浴时间对吸光值稳定性存在影响 ,且吸光值差异较大 ,同一浓度标管值吸光值在 0 3～ 0 6,鉴于此情况本文采用正交设计法对该实验方法最佳条件进行了优选 ,现报告如下。1　分析方法1 1　原理水溶性Ｔ…     

~(125)Ⅰ—血清结合胆酸放射免疫测定及对几种肝脏疾病诊断价值的探讨

胆酸在正常人血清中的浓度甚低,用一般化学分析方法难以测定。自放射免疫法测定血清胆酸建立后,不少学者探讨了血清胆酸浓度变化和肝胆疾病的关系。本文用~(125)Ⅰ-血清结合胆酸测定法测定了115例健康人和159例肝脏疾病患者的空腹血清结合胆酸浓度,以确定血清胆酸浓度正常值,并探讨~(125)Ⅰ-血清结合胆酸测定法对肝脏疾病的诊断价值,现将结果报告如下。     

奥昔布宁凝胶剂的制备和体外释药特性

目的:制备奥昔布宁凝胶剂,评价其体外释药性能。方法:以氮酮为促透剂,以羟丙甲基纤维素为凝胶基质,正交法设计处方,用扩散池进行奥昔布宁凝胶剂12h的经皮扩散试验,高效液相色谱法测定药物累计释放浓度,用时间滞留法求算经皮渗透参数。结果:奥昔布宁凝胶剂经度扩散参数分别为T_(lag)3.3h,D7.95E-07cm~2.h~(-1),Km20.91,Jss2.11E-04mmol.cm~(-2).h~(-1),12h体外累计释放量为(11.11±0.96)％。结论:奥昔布宁凝胶剂透过率高,渗透性好。     

紫外分光光度法测定饮用水中碘离子

目的探讨应用紫外分光光度法测定饮用水中碘离子的方法及测量条件的选择。方法利用溴水定量氧化I-生成IO3-,再用甲酸除去过量的溴,IO3-在过量碘化物存在下氧化I-产生3倍摩尔量的I3-,在紫外光区的286 nm具有最大吸收,并在345 nm处另有一略低的吸收峰,2ε86=1.3×105L.mol-1.cm-1,ε345=8.1×104L.mol-1.cm-1,均具有很高的灵敏度。结果I-浓度与其吸光值在10~100μg.L-1范围内成线性关系,标准曲线的相关系数分别为0.9990(286 nm处)和0.9991(345 nm处)。结论用于饮用水中微量碘离子的测定,方法简便、快速,结果准确。     

分光光度法测定地面水中微量敌枯双的研究

酸性条件下,TK5477经亚硝基化后,以萘乙二胺显色,用光度法测定含置。摩尔吸光系数(ε)为2.2×10~4、桑德尔灵敏度(S)是0.028μg/cm~2。吸光度为0.030时,检出浓度为0.5ppm,若低于此浓度时,用萃取光度法可提高灵敏度10倍。     

维生素C新分光光度测定法的研究—2,4—二硝基苯酚作显色剂

于0.8N氢氧化钠介质中及在85℃水浴中加热65分钟的条件下,维生素C与2、4—二硝基苯酚作用生成红色还原产物,其最大吸收波长位于470nm。维生素浓度介于132～329μg/ml之间遵守比耳定律,其表观摩尔吸光系数为662.4L.mol~(-1)cm~(-1),桑德尔灵敏度为0.2659μg/cm~2。红色还原产物的色泽可稳定24小时以上。本法曾用于药物针剂和片剂中维生素C含量的测定,获得满意的结果。     

多壁碳纳米管对小鼠肝和肺细胞蛋白质氧化损伤作用研究

目的 探索多壁碳纳米管对机体蛋白质的氧化损伤作用.方法 将20只老鼠随机分成4组,3个染尘组,1个对照组,用腹腔注射法进行1次性染尘,3个染尘组分别注入0.1、0.2、0.4 mg/ml的多壁碳纳米管(粒径20～40nm)颗粒悬浮液1ml.对照组注入等体积的生理盐水,染毒5天后测定肝脏和肺组织中的蛋白质羰基含量,比较不同浓度的多壁碳纳米管对肝和肺部蛋白质的氧化损伤作用.结果 不同浓度的碳纳米管染尘组小鼠与对照组相比较,肝脏组织蛋白质羰基含量在0.2mg/ml和0.4mg/ml染毒条件下都有显著性差异(P＜0.05).肺组织羰基含量在0.4mg/ml染毒条件下有极显著差异(P＜0.01).在其他浓度条件下都没有统计学意义(P＞0.05).结论 多壁碳纳米管对小鼠肝脏和肺部组织有一定的氧化损伤毒性作用.     

车间磷化氢测定方法初探

本文对车间空气中磷化氢进行测定。探讨了不同的硫酸浓度和不同的反应时间对吸光度的影响,在硫酸浓度为1mol/l,反应时间为10min时吸光值比较稳定。在0．25-1．75μg/ml范围内,吸光度与磷化氢标准溶液浓度线性关系良好,加标回收率为99．93％-100．52％。方法精密度为1．12％-2．98％。该方法准确、快速,可以用于车间空气中磷酸的测定。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.