豚鼠钩体病PDH模型血浆栓素和前列腺环素的测定

Guinea pigs were intravenously injected with icterhemorrhagiae serogroup Lai serovar strain 017 leptospirosis to model the pulmonary diffuse hemorrhage (PDH) in leptospirosis. Thirty-eight hours after the injection, the jugular arteries were catheterized to collect blood sample. The plasma was prepared for radioimmunoassay of TXB2 and 6-keto-PGF1a, the stable metabolites of TXA2 and PGI2 respectively. The plasma level of TXB2 in the experimental group, 107.15 +/- 41.65 pg/ml (n = 7), almost doubled that of the control, 54.05 +/- 12.93 pg/ml (n = 7), with significant difference (P less than 0.01); meanwhile, no significant difference was observed of 6-keto-PGF1a, 67.97 +/- 16.89 pg/ml (n = 6) vs. 98.06 +/- 40.63 pg/ml (n = 9) with P greater than 0.1. The fact that TXA2 causes vasoconstriction and increases vessel permeability suggests that TXA2 elevation should play a role in the mechanism of PDH in leptospirosis.     

Clinical Implication of the Changes of cAMP, TXA_2 and PGI_2 in CSF of Asphyxiated Newborns

Summary:To evaluate the changes of 3',5'-cyclic adenosine monophosphate(cAMP),thromboxane A_2(TXA_2)and prostacyclin(PGI_2)in cerebrospinal fluid(CSF)in the asphyxiated newborn andexplore their roles in hypoxic-ischamic brain damage(HIBD).Thirty-six full term newborns were di-vided into 3 groups,including 12 with moderate-severe hypoxic-ischaemic encephalopathy(HIE),13with mild HIE,11 without HIE(control group).The levels of cAMP,TXB_2(TXA_2 metabolite)and6-keto-PGF_(1α)(PGI_2 metabolite)in CSF and plasma were measured 36—72h after birth by RIA.andthe concentrations were expressed as nM/L(cAMP),ng/L(TXB_2 and 6-keto-PGF_(1α)).The infantswere followed-up at 6 and 12 month of age and Mental Development Index(MDI)and PsychomotorDevelopment Index(PDI)were measured using Bayley Scales of Infant Development(BSID).TheCSF cAMP level in moderate-severe HIE group was 8.60±2.40,significantly lower than that of themild HIE group(14.83±2.84)and the control group(24.43±2.39)(for both P<0.01).The lev-els of TXB_     

大鼠急性坏死性胰腺炎门静脉血中前列环素和血栓素变化

目的采用动物实验模型观察急性坏死性胰腺炎合并内脏损伤时门静脉血中血栓素（Thomboxane，TX）和前列环素（prostagtandin，PG）水平变化。方法用牛黄胆酸钠复制大鼠急性坏死性胰腺炎模型，放射免疫分析法测定血浆中TYB2和6－keto—PGF1α的含量来反映TXA2和PGI2的水平，并设对照组（假手术组）进行对照分析。结果实验组大鼠血浆中TYA2和PGI2水平较对照组高（P＜0．05），实验组大鼠腹腔静脉血TXA2和PGI2水平比门静止高（P＜0．05），PGVI2／TXA2比值下降资料处理采用t检验。结论TXA2PGI2是参与急性坏死性胰腺炎合并内脏损伤病理机制的重要因素之一。     

兔油酸型呼吸窘迫综合征TXB_2和PGI_2的变化及相互关系

本文对血栓素、前列腺环素在实验性呼吸窘迫综合征中的变化和相互关系进行了研究,并比较了肺动脉、左心房血浆和肺组织中的含量变化。实验分两组进行,Ⅰ组盐水对照,Ⅱ组实验组。动物注射油酸(0.045ml/kg)后,血浆及肺组织中的TXB_2(TXA_2的稳定代谢产物)立即升高,6-酮-PGF_1α(PGI_2的稳定代谢产物)则较晚上升,TBX_2/6-酮-PGF_1α先上升,后下降。结果提示:(1).TXA_2和PGI_2在油酸ARDS模型中是显著增加的;(2)血浆中与肺组织中的值有时不一致;(3)TXA_2/6-酮-PGF_1α在ARDS的发病机理中可能有重要作用。     

Clinical and experimental studies of coronary heart disease treated with yi-qi huo-xue injection

The purpose of the present work was to evaluate the clinical efficacy and the mechanism of Yi-qi Huo-xue Injection (YHI) in treatment of coronary heart disease. YHI consists of Ginseng, Astragalus and Angelicae Sinensis. The 10% dextrose serves as a placebo. The results were as follows: 1. the frequency and severity of angina episodes were reduced by 90.63%; 2. the ischemic ST-T in ECG was improved in 56.25% of cases; 3. the tolerance to treadmill exercise was increased from 348.50 to 503.50 M.; 4. the left ventricular function was strengthened, PEP/LVET ratio reduced from 0.45 to 0.36, the activity of (Na(+)-K+) ATPase in myocardial cell membrane of rats inhibited by 19.2%; 5. the blood viscosity and erythrocyte electrophoretic time lowered; 6. the adhesion and aggregation of platelet in patients with CHD were inhibited by 27% and 59.4% respectively; 7. the plasma TXB2 level in CHD was reduced from 260.28 +/- 164.4 to 139.29 +/- 57.01 pg/ml; 8. the plasma 6-keto-PGF1 alpha level in CHD was increased from 33.45 +/- 22.5 to 57.48 +/- 13.1 pg/ml, and in rats from 185.77 to 366.33 pg/ml. The differences were all statistically significant (P less than 0.05-0.01) in comparison with the placebo group.     

阻塞性黄疸大白鼠肾脏前列环素与血栓素的改变

本实验用放免方法测定雄性Sprague-Dewley大白鼠分别在结扎胆管1、2、3和4周后肾皮质与髓质组织中6-keto-PGF_(1α)及TXB_2含量来了解肾皮质与髓质中前列环素和血栓素A_2的变化。结合表明,肾皮质与髓质中6-Keto-PGF_(1α)在胆管结扎早期(1～2周)显著升高,而后期(3—4周)无继续升高。肾皮质与髓质中的TXB_2在结扎胆管后期显著升高,引起6-keto-PGF_(1α)/TXB_2比值在胆管结扎后期显著降低。提示胆管梗阻时间越长,肾组织中TXA_2合成增加越明显,导致肾脏易于出现血管痉挛,血小板聚集,肾循环功能障碍。     

肺缺血再灌注损伤时TXA2/PGI2平衡的变化及尼美舒利对其的影响

目的：探讨大鼠肺缺血再灌注损伤时TXA2/PGI2的变化及尼美舒利对其的影响。方法：健康SD大鼠30只,随机分为假手术组（S组）、缺血再灌注组（I/R组）和尼美舒利组（NIM组）,复制在体大鼠肺缺血再灌注损伤模型。检测血清丙二醛（MDA）含量、超氧化物歧化酶（SOD）和黄嘌呤氧化酶（XO）活力,放射免疫法测定肺组织血栓素B2（TXB2）、6-酮-前列腺素F1α（6-keto-PGF1α）含量并计算比值;逆转录聚合酶链反应（RT-PCR）检测肺组织环氧合酶-2 mRNA（COX-2 mRNA）的表达。结果：NIM组与IR组相比,血清MDA、XO均明显降低,SOD明显升高;TXB2明显下降（均P〈0.01）,6-keto-PGF1α无明显差异,COX-2 mRNA表达明显减弱（P〈0.01）。结论：尼美舒利可通过下调肺组织COX-2 mRNA的表达,抑制血小板释放TXA2,调控TXA2/PGI2的平衡,增强抗氧化应激而减轻肺缺血再灌注损伤。     

高压氧对激素性股骨头坏死兔血清TXA2、PGI2及血液流变学的影响

目的通过观察高压氧（HBO）治疗前后激素性股骨头坏死（SANFH）兔血清血栓素A2（TXA2）、前列腺素I2（PGI2）及血液流变学指标的变化,探讨HBO治疗SANFH的作用机制。方法健康新西兰白兔正常对照组10只、模型组20只[非干预组（N-HPO）和HPO组各10只],正常对照组和N-HPO组不予任何处理,高压氧组予以高压氧疗。检测并比较各组血清血栓素B2（TXB2,TXA2的稳定代谢产物）、6-酮-前列腺素F1α（6-keto-PGF1α,PGI2的稳定代谢产物）含量及血液流变学的变化。结果建模前模型组和对照组的血清TXB2、6-keto-PGFlot和血流变指标无明显差异（均P〉0．05）,建模成功后模型组的TXB2和血液流变学指标均显著高于对照组,而6-keto-PGF1α显著低于对照组,差异有统计学意义（均P〈0．01）;治疗前HPO组和N-HPO组的血清TXB2、6-keto-PGF1α和血液流变学指标无明显差异（均P〉0．05）,HPO治疗后2周、4周和6周模型组的TXB2和血液流变学指标均显著低于非HPO组,而6-keto-PGF1α显著高于非HPO组,差异有统计学意义（均P〈0．01）。结论HBO可纠正SANFH兔血中TXAJPGl2失衡,降低血黏度,减轻股骨头损伤。     

反义寡核苷酸对内毒素刺激的巨噬细胞血栓素A2合成的影响

OBJECTIVE: To select efficient fragments which can inhibit gene expression of thromboxane synthase and production of thromboxane A2. METHODS: Rat peritoneal macrophages were incubated with oligonucleotides (ODNs) and endotoxin (which was composed of lipopolysaccharide) for 24 hours. The TXB2 content was determined by RIA. Thromboxane synthase (TXS) mRNA expression was measured with RNA dot hybridization. RESULTS: The concentration of TXB2 in ODN I group was (69.7 +/- 10.9) pg/microgram cell protein, lower than those in control group (100.6 +/- 28.6) pg/microgram cell protein (P < 0.05, n = 4), TXS mRNA expression level in ODN I group was decreased as compared to those in control group. Neither in ODN II nor in ODN III group were there any changes on TXB2 contents and TXS mRNA levels. CONCLUSIONS: ODN I can inhibit the production of TXA2 and the gene expression of thromboxane synthase from macrophages stimulated by endotoxin.     

鼻咽癌患者前列环素及血栓素与外周血细胞免疫状况

应用间接免疫荧光法和放射免疫法分别检测了 34例鼻咽癌患者外周血中淋巴细胞的数量和血浆前列环素(PGI2 )及血栓素 (TXA2 )的含量 ,并与正常对照组对比研究。结果显示 :鼻咽癌患者外周血中 CD4细胞减少 (P<0 .0 1) ,而 CD8细胞则明显升高 (P<0 .0 5 )。同时鼻咽癌患者血浆中 PGI2 和 TXA2 的稳定代谢产物 6 - keto- PGF1α及 TXB2分别比对照组升高 ,以 TXB2 升高更明显 (P<0 .0 5 )。提示鼻咽癌患者体内前列腺素类物质 PGI2 和 TXA2 与鼻咽癌患者机体细胞免疫功能有关 ,并对其作为一种可能性抑制因素在鼻咽癌细胞免疫中的作用进行了讨论     

小剂量阿司匹林与培哚普利合用对兔血中前列环素、血栓素A2和去甲肾上腺素的影响

OBJECTIVE: To explore the interaction of low-dosage aspirin combined with angiotensin-converting enzyme (ACE) inhibitors by prostacyclin (PGI2), thromboxone A2 (TXA2) and norepinephrine (NE)) levels in rabbits' blood. METHODS: Forty healthy New Zealand rabbits were divided into four groups. Blood samples were drawn from the rabbits' heart before and after a consecutive four-week. NE was measured by high performance liquid chromatography, and PGI2 and TXA2 were measured by radioimmunoassay. RESULTS: ACE inhibitors increased PGI2 levels (P < 0.05, P < 0.01); low-dosage aspirin suppressed TXA2 productions (P < 0.05, P < 0.01) after the four-week administration. Aspirin combined with ACE inhibitors led to a significant increase in PGI2/TXA2(P < 0.01), together with a significant decrease in NE levels in the rabbits' blood (P < 0.001). CONCLUSION: Neither low-dosage aspirin nor ACE inhibitors influence NE levels alone. The ratio of PGI2 to TXA2 increased, and NE levels decreased significantly during the administration of aspirin combined with ACE inhibitors. The results suggest that there is a synergis-action between low-dosage aspirin and ACE inhibitors due to increased PGI2/TXA2 and decreased NE levels.     

Anti-angina effect of puerarin and its effect on plasma thromboxane A2 and prostacyclin

Plasma concentrations of thromboxane B2 (TXB2) and 6-keto-prostaglandin Fl alpha(6-K-PGFl alpha), the stable nonenzymatic metabolites of TXA2 and prostacyclin were assayed in 30 patients suffering from angina pectoris before and after administration of puerarin. In addition, serum lipids and HDL were also measured at the same time. 20 healthy subjects were chosen as the control group. Two weeks before and during administration of puerarin, aspirin, calcium-antagonists, all kinds of hypotensors and drugs relieving chest pain of angina pectoris were strictly prohibited. Puerarin was intravenously given, 500 mg daily for 7 days, which was considered as a therapeutic course. Besides relieving of chest pain, decreasing of heart rate and reduction of blood pressure clinically, it was also found that plasma 6-K-PGFl alpha concentrations were significantly elevated from 38.32 +/- 15.40 to 158. 79 +/- 98.62 pg (P less than 0.01) after administration of puerarin, but there was no significantly difference between plasma TXB2 concentrations before and after administering the drug. In addition, serum HDL was apparently enhanced as compared with that before the administration of puerarin (P less than 0.01). The results indicated that puerarin has the function of anti-angina, reducing both systolic and diastolic blood pressure and diminishing myocardial oxygen consumption.     

心病瘀血舌与TXB2、6-Keto-PGF1α的相关性研究

目的：从“TXA2-PGI2”平衡失调探讨心病瘀血舌形成的病理机制。方法：随机选择72例心病瘀血舌患者、30例心病非瘀血舌患者、30例非心病瘀血舌患者与20例健康人,采用RIA法检测血浆TXB2、6-Keto-PGF1α。结果：心病瘀血舌组的TXB2、TXB2/6-Keto-PGF1α显著高于对照组和心病非瘀血舌组（P〈0.01）,6-Keto-PGF1α显著低于对照组和心病非瘀血舌组（P〈0.01）。心病瘀血舌患者不同证型组的TXB2、TXB2/6-Keto-PGF1α均较对照组显著增高（P〈0.05,P〈0.01）,6-Keto-PGF1α均较对照组显著降低（P〈0.01）。结论：TXA2-PGI2失衡是心病瘀血舌形成的关键病理基础;综合观察TXB2、6-Keto-PGF1α、TXB2/6-Keto-PGF1α的异常程度可作为诊断心病瘀血舌的客观指标。     

PGI2,TXA2与先兆早产的关系研究

为探讨前列环酸（ＰＧＩ２）和血栓素（ＴＸＡ２）与先兆早产的关系，采用放射免疫法分别测定了先兆早产和足月临产孕妇血及宫颈粘液中６－酮－ＰＧＦ１α、ＴＸＢ２和孕酮的浓度，并与相对应孕周的孕妇进行比较。结果：与对照组相比，先兆早产组及足月临产组的血浆ＴＸＢ２和６－酮－ＰＧＦ１α水平明显升高（Ｐ〈０．００１），６－酮－ＰＧＦ１α／ＴＸＢ２比值降低（Ｐ〈０．００１），而孕酮水平则无显著性差异（Ｐ〉０．０５）     

Relation of pale tongue, purple tongue and TXA2-PGI2 regulation system

This paper analysed the relationship between pale tongue, purplish tongue and TXB2, 6-keto-PGF1 alpha levels in plasma of 70 cases with coronary heart disease (CHD) and 45 normal subjects. The results showed the following characteristics: The pale tongue group (217.76 +/- 30.5 pg/ml) showed no significant difference in TXB2 level compared with the normal group (164.49 +/- 10.85 pg/ml, P greater than 0.05), while both showed significant difference compared with the purplish tongue group (360.1 +/- 31.3 pg/ml) and that with purple spots (485.07 +/- 106.1 pg/ml, P less than 0.01). The pale tongue group (179.29 +/- 9.08 pg/ml) showed a significant difference in 6-keto-PGF1 alpha level compared with the normal group (244 +/- 19.31 pg/ml, P less than 0.01), but it showed no significant difference compared with the purplish tongue group (185.08 +/- 17.07 pg/ml) and that with purple spots (229.3 +/- 33.2 pg/ml, P greater than 0.05). The comparison between the groups of purplish tongue and that with purple spots and the normal group showed no significant difference (P greater than 0.05). The pale tongue group (1.33 +/- 0.18) showed a marked difference in TXB2/6-keto-PGF1 alpha ratio compared with the normal group (0.72 +/- 0.04, P less than 0.01), the purplish tongue group (2.12 +/- 0.22, P less than 0.01) and that with purple spots (2.25 +/- 0.55, P less than 0.05). The purplish tongue group and that with purple spots showed significant difference compared with the normal group (P less than 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)     

原发性慢性肾小球肾炎患者血浆6一Keto一PGF_(la)、TXB_2的变化

本文用放射免疫方法,测定了48例原发性慢性肾小球肾炎患者血浆6一Keto一PGF_(12)TXB_2的含量及T/6的比值,发现肾炎各型中血浆6一Keto—PGF_(12)含量均明显降低;TXB_2含量在肾病型明显增高,在肾功不全组明显降低。T/6比值在肾功正常各型中明显增高,在肾功不全组无明显改变。结果提示肾炎病变发展过程及,高凝状态的形成中,PCI_2-TXA_2的平衡失调起着重要的作用。为用能增加血浆中PGI_2或减少TXA_2产生的药物来治疗肾炎,提供依据。     

Effect of jiang-zhi zhong-yao-pian on total cholesterol, triglyceride, TXB2, 6-keto-PGF1 alpha in hyperlipemic patients

The purpose of this study was to verify the effect of a Chinese herbal medicine Jiang-Zhi Zhong-Yao-Pian to reduce serum lipoid. Efficacy was observed in 30 cases of hyperlipemia; 20 cases administered with evening primose oil capsules were taken as controls. Each group took drugs for two or three months. The results were as follows: After treatment as compared with before treatment, the serum levels of TC, TG and TXB2 dropped from 264.28 +/- 70.52 mg%, 393.52 +/- 250.42 mg% and 110.75 +/- 43.52 pg/ml to 225.60 +/- 50.93 mg%, 264.97 +/- 252.81 mg% and 88.82 +/- 46.50 pg/ml respectively (P less than 0.001, less than 0.01, less than 0.05). However, in the group taking evening primrose oil capsules, TC, TG and TXB2 in comparing with the pre-treatment levels were changed from 251.33 +/- 58.24 mg%, 316.35 +/- 104.93 mg% and 131.53 +/- 49.77 pg/ml to 244.30 +/- 43.28 mg%, 272.10 +/- 92.52 mg% and 115.33 +/- 47.49 pg/ml respectively (P greater than 0.05, less than 0.05, greater than 0.05). This medicine had no side-effect. The results showed that the herbal formula might be useful to reduce serum TC, TG and TXB2.     

原发性血小板增多症血小板功能变化及与血栓的关系

目的 观察原发性血小板增多症(essential thrombocythaemia,ET)患者血小板功能的变化及对血栓形成的影响,探讨奥扎格雷钠在ET患者中防治血栓发生的作用.方法 采用流式细胞仪(FCM)测定患者CD62P、PAC-1的水平;采用ELISA方法测定血浆血栓素A2(TXA2)代谢产物TXB2和前列环素(PG12)代谢产物6-K-PGF1α水平;观察奥扎格雷钠干预治疗对ET患者血小板活性及血栓发生率的影响.结果 治疗前两组ET患者CD62P、PAC-1、TXB2、6-keto-PGF1α和TXA2/PGI2指标均明显高于正常组(P<0.01);奥扎格雷钠干预治疗后CD62P、PAC-1、TXB2、TXA2/PGI2均较治疗前明显降低(P<0.01),奥扎格雷钠干预治疗后CD62P、PAC-1和TXA2/PGI2与正常相比差异无统计学意义,而TXB2、6-keto-PGF1α仍高于正常,差异有统计学意义(P<0.05),常规治疗组治疗后与治疗前相比,各项指标均无明显变化.奥扎格雷钠干预治疗组后期血栓发生率为12.5%,常规治疗对照组血栓发生率为54.1%,差异有统计学意义(P<0.05).结论 ET患者存在血小板异常活化现象和一定的血栓形成危险,临床上除常规治疗减少血小板数量外,还应重视血小板功能的干预治疗,以改善血小板功能、防止血栓发生.     

异丙酚对大鼠血流动力学和血浆中血管内皮因子的影响

目的　探讨异丙酚对大鼠血流动力学和血浆一氧化氮 (NO)、内皮素 (ET)、前列环素 (PGI2 )、血栓素A2 (TXA2 )的影响。　方法　 12～ 14周 Wistar大鼠 2 4只 ,随机分为 3组 ,每组 8只。 A组 (对照组 ,注射 0 .9%生理盐水 ) ;B组 (低剂量异丙酚 )和 C组 (高剂量异丙酚 )静注异丙酚 5 mg/ kg诱导麻醉后分别静脉持续 30 min注入异丙酚 30 ,6 0 mg· kg- 1 · h- 1 ,总容量均相同。记录不同时间的收缩压 (SBP)、平均动脉压 (MAP)、舒张压 (DBP)、心率 (HR)及呼吸 ,测定血浆 NO、ET、PGI2 、TXA2 的含量。　结果　 (1)血流动力学改变 :组内比较 ,B组血压、心率变化不显著 (P>0 .0 5 ) ;C组随时间的延长 ,SBP、MAP、DBP、HR均下降 ,尤以 30 min为著 (P<0 .0 5或 P<0 .0 1)。组间与 A组比较 :各组基础值无差异 ;B组仅在 2 min、10 m in时 HR下降 (P<0 .0 5 ) ;而 C组 SBP、MAP、DBP、HR均下降 (P<0 .0 5或 P<0 .0 1) ,且 HR随时间的延长与 A组的差异明显增加 ,30 m in时 P<0 .0 0 1。 (2 )两组血浆中NO、PGI2 增高 ,ET、TXA2 下降 ,NO/ ET及 PGI2 / TXA2 的比例增高 ,C组改变显著。　结论　异丙酚呈时间剂量依赖性降低血压减慢心率 ;促进 NO、PGI2 的释放 ,抑制 ET、TXA2 的释放。NO/ ET,PGI2 / TXA2 的失衡与异     

光量子氧疗对脑梗死患者TXA2-PGI2平衡及脑血流的影响

目的旨在观察光量子氧疗对脑梗死患者血栓素A2 前列腺素I2 平衡及脑血流的影响。方法将脑梗死后 3～ 10周患者 10 8例随机分为两组。A组予丹参液静脉滴注 ,B组予充氧光量子化丹参液静脉滴注 2周。治疗前、后分别做神经功能缺损程度评分、测定血浆血栓素B2 (thromboxaneB2 ,TXB2 )和 6 酮 前列腺素F1α(6 k PGF1α)含量、经颅血流多普勒超声测定大脑中动脉血流的平均峰流速 (meanvelocity ,Vm )并计算两侧的非对称指数。结果治疗前后两组的神经功能缺损程度评分均显著降低 ,但B组降低的幅度显著大于A组。B组的总有效率显著高于A组。两组血浆TXB2 和TXB2 / 6 k PGF1α比值在治疗后均显著降低 ,但B组降低的幅度显著大于A组。而且B组在治疗后 6 k PGF1α显著上升 ,而A组变化不显著。两组患侧Vm治疗后均显著增加 ,但B组增加的幅度显著大于A组。而且B组在治疗后Vm的非对称指数显著降低 ,非对称性病例显著减少 ,而A组变化不显著。血浆TXB2 、TXB2 / 6 k PGF1α比值与患侧Vm之间均呈显著负相关。结论光量子氧疗可显著促进纠正脑梗死患者恢复期的TXA2 PGI2 失衡 ,并加速患侧大脑中动脉血流的恢复 ,光量子氧疗的临床疗效可能与这些作用有关