我国真江蓠生物活性物质PG的检出

对青岛沿海产海藻真江蓠为原料,从中提取计划生育用药－前列腺素类活性物质,经与对照品的薄层层析及高压液相图谱对照分析,初步鉴定为前腺素Ｅ２（ＰＧＥ２）。     

南海豆荚软珊瑚化学成分的研究(Ⅰ)

从中国南沙海域采集的豆英软珊瑚中分离得到１个神经酰胺。利用ＮＭＲ,ＩＲ,ＭＳ等波谱和光谱技术,确定了它的结构为△＾４．５（Ｅ）,△＾８,９（Ｅ）－鞘胺醇－正十六碳酰胺。     

海洋红藻松节藻Rhodomela confervoides化学成分研究

目的：对采自青岛海滨的海洋红藻松节藻Rhodomela confervoides进行化学成分研究。方法：各种色谱柱层析和IR、MS、^1H—NMR、^13C—NMR、DEPT等波谱解析技术。结果：从松节藻中分离得到5个化合物，其结构经过鉴定分别为：2，2′，3，3′四溴—4，4′，5，5′四羟基双苯基甲烷(I)、2，2′，3—三溴—3′，4，4′，5—四羟基—6′甲氧甲基双苯基甲烷(Ⅱ)，2，3—二溴—4，5—羟基苯甲醇(Ⅲ)、2，3—二溴—4，5—二羟基—苯甲醛(Ⅳ)、2，3—二溴—4，5—二羟基苯甲基乙基醚(Ⅴ)。其中化合物Ⅱ对KB和Bel7402肿瘤细胞有选择性细胞毒活性。     

红藻海头红药效学及化学成分的研究(Ⅰ)

对红藻门海藻海头红的不同极性部位进行了抗肿瘤活性研究 ,其中石油醚部位在 12 5～ 50 0 mg·kg-1剂量范围内 ,对小鼠 S180 肉瘤、H2 2 肝癌、艾氏腹水癌均具有明显的抑制作用 ,并具有一定的剂量依赖性 ,在对肿瘤生长产生抑制作用的同时 ,不影响动物的体重增长。对人宫颈癌 He L a细胞、白血病 HL60 细胞也具有一定的体外细胞毒作用 ;氯仿部位及正丁醇部位抑瘤作用稍差 ,水部位毒性较大。对海头红全藻进行了化学成分的分离 ,现已从石油醚部位分离得到 5个化合物 ,经化学方法和光谱分析鉴定了其中 4个化合物的结构 ,分别为 β-谷甾醇 ( )、硬脂酸 ( )、十八烷酸三十四烷醇酯 ( )和 β-谷甾醇十六烷酸酯 ( )。     

南海海绵Clathria f asciculate化学成分的研究(Ⅰ)

从中国南海海绵Clathria fasciculate的乙酸乙酯可溶部分分离获得11个纯有机化合物，经Lieber-mann-Burchard试验和IR、^1H-NMR、`13C－NMR、DEPT、EIMS等波谱技术，确定其中的3个为甾醇类化合物，分别是：胆甾－6－烯－5α，8α－环二氧－3β－醇（Ⅰ）；（24s）－豆甾－5－烯－3β－醇（Ⅱ）和胆甾醇（Ⅲ）。其余几个化合物的结构仍在鉴定之中。     

细基江篱的化学成分

目的研究红藻细基江篱(Gracilaria tenuistipitata)中的化学成分。方法细基江篱用甲醇冷浸提取,浓缩成浸膏,分别用石油醚、乙酸乙酯萃取,所得石油醚部分和乙酸乙酯部分利用反复硅胶柱色谱、凝胶柱色谱等方法进行分离纯化,并根据其理化性质和光谱(NMR及MS等)数据进行化学结构鉴定。结果从细基江篱的石油醚和乙酸乙酯部分共分离得到了8个化合物,经鉴定分别为胆甾醇(1)、3β羟基胆甾5烯7酮(2)、胆甾5烯3β,7α二醇(3)、β谷甾醇(4)、胡萝卜苷(5)、胸腺嘧啶(6)、尿嘧啶(7)、棕榈酸(8)。结论上述化合物均为首次从该种海藻中分离得到。     

中国南海软珊瑚的化学成分研究

报道从中国南海软珊瑚中分离到的两个１９－羟基－２４亚甲基多羟基甾醇－－３β，７β，１９－三羟基－２４－亚甲基胆甾醇和３β，１９－二羟基－２４－亚甲基胆旮醇的结构鉴定，此外，还简要报道２４－亚甲基胆甾醇和鲨肝醇的分离鉴定。     

赤芝子实体化学成分研究和细胞毒活性测试

目的研究赤芝子实体石油醚部位的化学成分,寻找具有抗肿瘤活性的先导化合物。方法采用硅胶、Sephadex LH-20等柱色谱以及半制备高效液相色谱等手段分离纯化,并通过理化性质和波谱数据鉴定化合物的结构;采用MTT法测试分离化合物对人宫颈癌细胞的细胞毒活性。结果从赤芝子实体石油醚部位分离得到9个化合物,分别鉴定为3,7-二羟基-12-乙酰氧基-11,15,23-三羰基羊毛甾-8,22-二烯-26-酸(12-acetoxy-3,7-dihydroxy-11,15,23-trioxolanost-8,22-dien-26-oic acid,1)、灵芝三醇M(ganoderitriol M,2)、亚油酸乙酯[(10E,13E)-ethyl octadeca-10,13-dienoate,3]、亚油酸[(10E,13E)-octadeca-10,13-dienoic acid,4]、二十四碳酸(tetracosanoic acid,5)、3β-羟基-5α,8α-过氧麦角甾-6,9,22-三烯(3β-hydroxy-5α,8α-epidioxyergosta-6,9,22-triene,6)、麦角甾-7,22-二烯-3-酮(ergosta-7,22-dien-3-one,7)、过氧麦角甾醇(ergosterol peroxide,8)和麦角甾醇(ergosterol,9)。结论化合物1的波谱数据为首次报道,化合物3、4、7为首次从赤芝中分离得到。化合物2显示出中等强度的癌细胞抑制活性,其IC50为12μmol·L-1。     

细枝竹节柳珊瑚化学成分研究

采自我国南沙群岛的细枝竹节柳珊瑚(Isis minorbrachlasta)的乙醇浸膏经硅胶快速层析,分离得到4个化合物,经波谱分析鉴定为柳珊瑚甾醇、22—epihippuristanol、神经酰胺和鲨肝醇。     

Chemical constituents from the red alga Symphyocladia latiuscula

The extraction and solvent fractionation of red alga Symphyocladia latiuscula, and repeated column chromatography led to isolation of 17 compounds: cholesterol (1), 3β,5β-dihydroxy-B-norcholestan-6β-carboxaldehyde(2),6β-hydroxy-cholest-4-ene-3-one(3), 1-O-myristoyl-3-O-(6′-sulfo-α-D-quinovopyranosyl)glycerol (4), 1-O-palmitoyl-3-O-(6′-sulfo-α-D-quinovopyranosyl)glycerol (5), 1-O-palmitoyl-3-O-[α–D-galactopyranosyl(1→6)β-D-galactopyranosyl] glycerol(6), 1-O-palmitoyl-3-O-β-D-galactopyranosylglycerol (7), methyl hexadecanoate (8), methyl stearate(9), hexadecanoic acid (10), γ-n-butyl cis-aconiate (11), α-n-Butyl cis-aconiate (12), phenethylamine (13),3,5-dibromo-L-tyrosine (14), 3-methylbutylamine (15),methyl pyroglutamate (16),n-butyl pyroglutamate (17).The structures of these compounds were identified by NMR spectroscopy and mass spectrometry, and compared with those reported in the literature. All the compounds were isolated from Symphyocladia genus for the first time.These compounds were investigated for their inhibitory effects on human recombinant aldose reductase in vitro. Of the compounds, 1-O-palmitoyl-3-O-β-D-galactopyranosylglycerol (7) demonstrated moderate enzyme inhibition.     

Chemical constituents of the red alga Laurencia tristicha

Six new sesquiterpenes, 10-hydroxy-epiaplysin (1), 10-hydroxy-aplysin (2), 10-hydroxy-debromoepiaplysin (3), aplysin-9-ene (4), epiaplysinol (5) and debromoepiaplysinol (6), together with 13 known compounds (7–19), have been isolated from the red alga Laurencia tristicha. The structures of 1–6 were determined by spectroscopic methods including IR, EI-MS, HREI-MS, and 1D and 2D NMR techniques. All compounds were obtained from this species for the first time and were tested for cytotoxic activities against several human cancer cell lines including lung adenocarcinoma (A549), stomach cancer (BGC-823), hepatoma (Bel 7402), colon cancer (HCT-8) and HeLa cell lines. Compound 6 showed selective cytotoxicity against HeLa cell line with IC₅₀ 15.5 μM, cholest-5-en-3β,7α-diol (14) was toxic to all tested cell lines with IC₅₀ values of 16.8, 5.1, 0.5, 0.5, and 0.3 μM, respectively, and other compounds were inactive (IC₅₀>10 μg/ml).     

Chemical constituents of the red alga Laurencia tristicha

Six new sesquiterpenes, 10-hydroxy-epiaplysin (1), 10-hydroxy-aplysin (2), 10-hydroxy-debromoepiaplysin (3), aplysin-9-ene (4), epiaplysinol (5) and debromoepiaplysinol (6), together with 13 known compounds (7-19), have been isolated from the red alga Laurencia tristicha. The structures of 1-6 were determined by spectroscopic methods including IR, EI-MS, HREI-MS, and 1D and 2D NMR techniques. All compounds were obtained from this species for the first time and were tested for cytotoxic activities against several human cancer cell lines including lung adenocarcinoma (A549), stomach cancer (BGC-823), hepatoma (Bel 7402), colon cancer (HCT-8) and HeLa cell lines. Compound 6 showed selective cytotoxicity against HeLa cell line with IC(50) 15.5 microM, cholest-5-en-3beta,7alpha-diol (14) was toxic to all tested cell lines with IC(50) values of 16.8, 5.1, 0.5, 0.5, and 0.3 microM, respectively, and other compounds were inactive (IC(50)>10 microg/ml).     

Chemical constituents of Centella asiatica

Studies on the chemical constituents of the aerial part of Centella asiatica have led to the isolation of three new compounds, named centellin (1), asiaticin (2), and centellicin (3). Their structures have been elucidated through spectral studies including 2D NMR experiments (HMQC, HMBC, (1)H-(1)H COSY, NOESY and J resolved).     

Chemical constituents of Centella asiatica

Studies on the chemical constituents of the aerial part of Centella asiatica have led to the isolation of three new compounds, named centellin (1), asiaticin (2), and centellicin (3). Their structures have been elucidated through spectral studies including 2D NMR experiments (HMQC, HMBC, ¹H–¹H COSY, NOESY and J resolved).     

Chemical constituents of Centella asiatica

Studies on the chemical constituents of the aerial part of Centella asiatica have led to the isolation of three new compounds, named centellin (1), asiaticin (2), and centellicin (3). Their structures have been elucidated through spectral studies including 2D NMR experiments (HMQC, HMBC, ¹H-¹H COSY, NOESY and J resolved).     

Chemical constituents of the brown alga Dictyopteris divaricata

A novel sesquiterpene-substituted benzoic acid, named dictyvaric acid (1), together with nine known compounds (2–10), have been isolated from the brown alga Dictyopteris divaricata Okam. The structure of 1 was elucidated as 3-[(decahydro-2-hydroxy-2,5,5,8a-tetramethyl-1-naphthalenyl)-methyl]-4-hydroxybenzoic acid by spectroscopic methods, including IR, FABMS, HR-FABMS, 1D and 2D NMR techniques. All compounds were obtained from this species for the first time.     

Chemical constituents of the brown alga Dictyopteris divaricata

A novel sesquiterpene-substituted benzoic acid, named dictyvaric acid (1), together with nine known compounds (2-10), have been isolated from the brown alga Dictyopteris divaricata Okam. The structure of 1 was elucidated as 3-[(decahydro-2-hydroxy-2,5,5,8a-tetramethyl-1-naphthalenyl)-methyl]-4-hydroxybenzoic acid by spectroscopic methods, including IR, FABMS, HR-FABMS, 1D and 2D NMR techniques. All compounds were obtained from this species for the first time.     

褐藻羊栖菜Sargassum fusiforme (Harv.) Setch.化学成分研究(II)Δ*

目的 研究褐藻羊栖菜Sargassum fusiforme的化学成分。方法：采用大孔吸附树脂柱色谱、硅胶柱色谱、ODS柱色谱和制备HPLC等技术方法进行化学成分分离,运用核磁共振波谱和质谱等数据,并结合文献鉴定化合物的结构。结果：从羊栖菜乙醇提取物中分离得到10个化合物,分别鉴定为对羟基苯甲醛(1)、2,5-二羟基苯甲醛(2)尿黑酸(3)、尿黑酸乙酯(4)、对羟基苯甲酸(5)、5-乙氧基甲基尿嘧啶(6)、黑麦草内酯(7)、异黑麦草内酯(8)、schiffnerilolide(9)、焦谷氨酸乙酯(10)。结论：化合物1~10为首次从羊栖菜中分离得到。