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目的:探讨生长激素释放肽(Ghrelin)促进乳腺癌细胞MDA-MB-231增殖的分子机制。方法:乳腺癌细胞MDA-MB-231经Ghrelin、Ghrelin受体(growth hormone secretagogue receptor,GHSR)抑制剂[D-Lys3]-GHRP-6或哺乳动物雷帕霉素靶蛋白(mammalian target of Rapamycin,mTOR)抑制剂雷帕霉素(Rapamycin)处理后,MTT或BrdU实验检测MDA-MB-231细胞的增殖能力;Western blot检测MDA-MB-231细胞GHSR表达及mTOR、p70S6K和S6磷酸化水平。结果:增殖实验结果表明Ghrelin增强MDA-MB-231细胞增殖能力;Western blot检测发现Ghrelin激活MDA-MB-231细胞mTOR、p70S6K和S6磷酸化,[D-Lys3]-GHRP-6或Rapamycin消除Ghrelin促进MDA-MB-231细胞增殖效应,同时抑制Ghrelin诱导的mTOR、p70S6K和S6磷酸化。结论:Ghrelin通过与GHSR结合激活MDA-MB-231细胞mTOR/p70S6K/S6信号途径促进MDA-MB-231细胞增殖。 相似文献
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Yan-jun Zhang Lichun Wei Mei Liu Jie Li Yi-qiong Zheng Ying Gao Xi-ru Li 《Tumour biology》2013,34(3):1605-1613
The purposes of this study were to investigate the effects of B cell translocation gene 2 (BTG2) on the proliferation, apoptosis, and invasion of triple-negative breast cancer and to provide an experimental basis for the future treatment of human triple-negative breast cancer. A pcDNA3.1-BTG2 eukaryotic expression vector was constructed and transfected into the MDA-MB-231 human triple-negative breast cancer cell line using lipofection. Then, relevant changes in the biological characteristics of the BTG2-expressing cell line were analyzed using MTT (tetrazolium blue), flow cytometry, and Transwell invasion chamber assays. Additionally, the effects of BTG2 expression on cyclin D1, caspase 3, and matrix metalloproteinases 1/2 (MMP-1/-2) expression were analyzed. Cell proliferation was significantly lower in the pcDNA3.1-BTG2-transfected group compared to the empty vector and blank control groups (p?<?0.05). There was no significant difference between the empty vector and blank control groups. FCM results demonstrated that there were significantly more cells in the G1 phase of the cell cycle and fewer S phase cells in the pcDNA3.1-BTG2 group than in the empty vector and blank control groups (p?<?0.05). Additionally, the proportion of cells that migrated across the membrane was significantly lower in the pcDNA3.1-BTG2 group than in the empty vector and blank control groups (p?<?0.05). Cyclin D1 and MMP-1/-2 expression were significantly lower in MDA-MB-231 cells transfected with pcDNA3.1-BTG2 as compared to the empty vector and blank control groups (p?<?0.05). Caspase 3 expression was significantly higher in MDA-MB-231 cells from the pcDNA3.1-BTG2 group compared to the empty vector and blank control groups (p?<?0.05). In conclusion, BTG2 may inhibit MDA-MB-231 proliferation and promote apoptosis. Additionally, BTG2 may also inhibit the invasion of MDA-MB-231 human triple-negative breast cancer cells. 相似文献
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目的 应用短发夹RNA(short hairpin RNA,shRNA)抑制乳腺癌MDA—MB-231细胞胰岛素样生长因子1型受体(type I insulin—like growth factor receptor,IGF-1R)的表达,探讨IGF~1R对乳腺癌体外增殖和迁移的作用。方法构建携带有绿色荧光蛋白报告基因的IGF-1R shRNA质粒载体,脂质体介导转染MDAMB-231细胞,通过倒置荧光显微镜检测转染效率,CCK-8法检测细胞增殖能力,体外迁移实验检测细胞迁移能力。细胞增殖实验结果的组间比较采用重复测量方差分析和多元方差分析,RT—PCR定量结果和迁移实验结果的组间比较采用单因素方差分析。结果成功构建IGF-1RshRNA质粒载体,转染效率为55%~60%。IGF-1R shRNA转染MDA—MB-231细胞后,MDA—MB-231细胞IGF-1R mRNA与蛋白表达水平显著下降;细胞体外增殖和迁移能力受到显著抑制(P均〈O.05)。结论IGF-1R shRNA表达质粒可以有效抑制MDA—MB-231细胞IGF-1R的表达,显著抑制乳腺癌细胞的体外增殖和迁移能力。 相似文献
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桔梗皂苷D诱导乳腺癌MDA-MB-231细胞凋亡 总被引:1,自引:0,他引:1
目的:研究来自桔梗的天然单体化合物桔梗皂苷D(platycodin D,PD)对高转移性乳腺癌细胞MDA-MB-231凋亡的影响,初步探索其可能的作用机制.方法:以不同浓度(0、2.5、5、10 μmol/L)PD处理乳腺癌MDA-MB-231细胞后,采用MTT法检测细胞增殖率并计算IC50值,流式细胞术检测细胞凋亡情况,Western blotting检测凋亡相关蛋白的表达水平.结果:PD呈剂量依赖性显著抑制MDA-MB-231细胞的增殖(P<0.01),作用72 h的IC50值为(7.30±2.67) μmol/L.与对照组相比,10 μmol/L的PD可显著促进MDA-MB-231细胞的凋亡(P<0.05).PD激活了caspase家族蛋白,上调有活性的cleaved caspase-3、cleaved caspase-8和cleaved caspase-9的表达,下调无活性的caspase-8和caspase-9的表达;PD同时减少Bcl-2的表达,增加Bax的表达,使Bcl-2/Bax的比值降低.研究还发现PD使突变型P53蛋白的表达减少、E2F1的表达增加.结论:PD抑制乳腺癌细胞增殖具有明显的抗肿瘤效应,而诱导凋亡的发生可能是其发挥抗肿瘤效应的机制之一. 相似文献
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Genipin, a constituent of Gardenia jasminoides Ellis, is used in the treatment of hepatic disorders and inflammatory diseases in traditional medicine. Although mounting evidence suggests an anti-tumor activity of genipin in several cancer cell systems, the inhibitory effect of genipin on the growth of breast cancer cells has not been reported yet. The present study aimed to investigate the anti-proliferative activity of genipin in MDA-MB-231 human breast cancer cells. Herein, we showed that genipin efficiently induced apoptosis in MDA-MB-231 cells by the down-regulation of Bcl-2, up-regulation of Bax and proteolytic activation of caspase-3. Activation of JNK and p38 MAPK also increased by genipin. Importantly, genipin significantly inhibited invasive and migratory phenotypes of MDA-MB-231 cells. Taken together, this study demonstrates that genipin induces apoptosis and inhibits invasive/migratory abilities of highly invasive MDA-MB-231 human breast cancer cells, suggesting a potential application of genipin as a chemopreventive agent that may prevent or alleviate metastatic breast cancer. 相似文献
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Roscovitine induces cell death and morphological changes indicative of apoptosis in MDA-MB-231 breast cancer cells 总被引:4,自引:0,他引:4
We have previously shown (Mgbonyebi et al., Anticancer Res., 18: 751-756, 1998) that roscovitine, an olomoucine-related purine analogue and a selective inhibitor of cyclin-dependent kinases, inhibited the proliferative activity of human breast epithelial cells in vitro. The purpose of the present study was to identify the cellular processes and targets affected by roscovitine treatment in the estrogen receptor-negative MDA-MB-231 human breast carcinoma cells. Treatment of the cells with 10 microg/ml roscovitine daily for a length of time ranging from 24 to 240 h revealed that the compound inhibited DNA synthesis, induced cell death, and irreversibly inhibited the proliferative activity of the cells. Morphological analysis of roscovitine-treated cells by light and fluorescence microscopy demonstrated that this cyclin-dependent kinase inhibitor induced cell shrinkage, chromatin condensation, reorganization of actin microfilament architecture, and extensive detachment of cells from the cell culture substratum. These cellular events are all known to be associated with apoptosis. Collectively, the data generated from this study suggest that roscovitine induced apoptosis in the estrogen receptor-negative MDA-MB-231 human breast cancer cells. Because the efficacy of many anticancer drugs depends on their ability to induce apoptotic cell death, modulation of this parameter by roscovitine may provide a new chemopreventive and chemotherapeutic strategy for the clinical management of hormone-resistant breast cancers. 相似文献
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目的 探讨黄芪注射液作用于basal-like型乳腺癌MDA-MB-231细胞株后对其增殖和凋亡的影响.方法 将实验细胞分为对照组和5种不同剂量的黄芪注射液作用组.用MTT法检测细胞增殖,流式细胞仪分析细胞周期,碘化丙啶染色检测细胞凋亡率.黄芪注射液作用于MDA-MB-231细胞48 h和72 h的OD值采用多个均数比较的方差分析;48 h时细胞周期各阶段细胞比率的比较用χ2检验.结果 作用48 h时,各剂量组均可抑制MDA-MB-231细胞增殖(P<0.01),其效应呈质量浓度依赖性.作用48 h时,高剂量组(2×10-1~2×10-2 g/ml)黄芪注射液还可促进细胞凋亡(χ2=8.01,P=0.00);作用72 h时,高剂量组仍呈抑制作用,但随质量浓度的降低,抑制作用减少,低剂量黄芪组(2×10-4 ~2×10-5 g/ml)有促进增殖作用(P<0.01);中高剂量组(2×10-3 g/ml)可改变细胞周期的分布.结论 黄芪注射液可抑制MDA-MB-231细胞增殖,并诱导其凋亡,可能为basal-like乳腺癌的治疗带来益处.. 相似文献
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目的:研究不同浓度洋地黄对体外培养的人乳腺癌细胞株MDA-MB-231增殖和凋亡的影响,并探讨其可能机制。方法:取对数生长的细胞,不同浓度洋地黄处理,MTT法观察洋地黄对MDA-MB-231细胞活性、增殖的影响;Hoechst 33342染色观察洋地黄促细胞凋亡作用,细胞色素C含量及caspase 9活性变化检测线粒体损伤程度;生长曲线法观测洋地黄对细胞增殖的影响,Western blot检测细胞周期蛋白表达。结果:高浓度的洋地黄(≥100nmol/L)引起MDA-MB-231细胞凋亡,促进细胞色素C释放,增加caspase 9活性。低浓度洋地黄(30nmol/L)不引起肿瘤细胞凋亡,但是抑制细胞增殖,抑制细胞周期素D1和细胞增殖抗原PCNA的表达,增强p21cip1蛋白的表达。结论:高浓度洋地黄通过线粒体损伤通路促进乳腺癌细胞凋亡,低浓度洋地黄通过干扰细胞周期抑制乳腺癌细胞的生长。 相似文献
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Beta-sitosterol,a plant sterol,induces apoptosis and activates key caspases in MDA-MB-231 human breast cancer cells 总被引:1,自引:0,他引:1
The objective of the present study was to evaluate the effect of beta-sitosterol, a plant sterol that induces apoptosis in breast cancer cells, on two pathways leading to apoptosis. These pathways are classified based on the localization of the initiated signal, extrinsic and intrinsic pathways. Extrinsic and intrinsic pathways are catalyzed by caspases 8 and 9, respectively, which leads to the activation of the executioner caspase 3. The results of the present study indicate that beta-sitosterol supplementation at 16 microM for 3 days to MDA-MB-231 cells induces 39% and 80% increases in the activities of caspases 8 and 9, respectively, compared to cholesterol supplemented cells or controls. There was also a 3-fold increase in the activity of caspase 3. Sterol treatment had no effect on the quantities of the enzymes. It is concluded that beta-sitosterol may induce apoptosis through the two pathways but was more pronounced on the intrinsic pathway. 相似文献
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目的 观察AZD2281对乳腺癌MDA-MB-231细胞株增殖和凋亡的影响,并探讨其可能的机制。方法MTT法和流式细胞仪法观察不同浓度AZD2281(2.5、5、10 nmol/L)作用于MDA-MB-231细胞24、48、72 h后对细胞增殖、周期分布及细胞凋亡的影响;Western blotting检测经AZD2281处理该细胞24 h后细胞凋亡相关蛋白Bcl-2和caspase-3表达水平的变化。结果AZD2281可显著抑制MDA-MB-231细胞增殖,且呈时间和剂量依赖性;AZD2281作用于MDA-MB-231细胞24 h后,细胞被阻滞在G0/G1期,并促进其凋亡,且呈剂量依赖性。AZD2281作用MDA-MB-231细胞24 h后,凋亡抑制蛋白Bcl-2的表达呈剂量依赖性下降,而凋亡促进蛋白caspase-3的表达呈剂量依赖性上升。结论AZD2281能显著抑制MDA-MB-231细胞增殖和促进其凋亡,其作用机制可能是通过上调caspase-3和下调Bcl-2蛋白表达实现的。 相似文献
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目的:探讨洛伐他汀对人乳腺癌细胞MDA-MB-231的作用及相关机制。方法:选用人乳腺癌MDA-MB-231细胞进行体外培养,采用MTT比色法俭测细胞增殖,测定其吸光度(OD)值。流式细胞仪测定细胞周期和凋亡率,光学显微镜和荧光显做镜进行形态学观察,流式细咆仪间接免疫荧光技术分析细胞内bcl-2蛋白、cdk2蛋白的表达。结果:不同浓度洛伐他汀处理不同时间后,MDA-MB-231细胞增殖明显受到抑制,抑制率最高可达77%,且同一处理时相点各组间比较差异均有显著性。洛伐他汀作用MDA-MB-231后,出现细胞凋亡峰和凋亡率的升高。细胞期分析显示,各组处理72小时,G0/G1期细胞增多,S期细胞减少,G2/M期细胞增加,而H细胞周期分布特点与药物浓度有关。用流式细胞仪间接分析细胞内bcl-2、cdk2蛋白的表达率显示:洛伐他汀处理组bcl-2、cdk2蛋白表达低于对照组,差异有显著性。结论:洛伐他汀对人乳腺癌MDA-MB-231细咆有明显的抑制增殖和诱导凋亡作用,bcl-2蛋白、cdk2蛋白表达下降可能为其分子机制之一。 相似文献
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目的:构建CXC趋化因子受体4(CXC chemokine receptor 4, CXCR4)RNA干扰真核表达载体,研究其对人乳腺癌细胞MDA-MB-231增殖、黏附及迁移能力的抑制作用。方法:构建针对CXCR4的带发夹结构的小RNA干扰序列,并连接到pGCsi-U6-Neo-GFP载体中,转染293T细胞,筛选出干扰效率最高的表达载体。脂质体法转染MDA-MB-231细胞。利用CCK8法、细胞-基质黏附实验和划痕修复实验检测shRNA干扰CXCR4表达对MDA-MB-231细胞增殖、黏附和迁移能力的影响。结果:成功构建CXCR4-shRNA重组质粒,并转染293T细胞,利用RT-PCR及Western blotting检测发现CXCR4沉默效率最高可达81.3%。CXCR4-shRNA转染能显著抑制MDA-MB-231细胞的增殖(P<0.05)以及细胞与细胞外基质的黏附(P<005)。CXCR4-shRNA转染组MDA-MB-231细胞的迁移距离明显低于对照质粒组和空白对照组(P<0.01)。结论:CXCR4-shRNA干扰载体能特异性抑制CXCR4的表达,从而抑制乳腺癌MDA-MB-231细胞的增殖、黏附及迁移。 相似文献
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背景与目的:Celecoxib可以抑制多种肿瘤细胞增殖、诱导其凋亡,但具体的作用机制尚不明确.本研究探讨Celecoxib是否通过阻断NF-kB信号途径诱导人乳腺癌MDA-MB-231细胞的凋亡.方法:RT-PCR法检测MDA-MB-231细胞中COX-2 mRNA的表达.MTT法检测Celecoxib、PGE2与Celecoxib联合应用对MDA-MB-231细胞增殖的影响.流式细胞术检测细胞周期、凋亡的变化.Westernblot法检测0、40、80、120 μmol/L Celecoxib作用MDA-MB-231细胞24 h后细胞中Caspase-3、p65蛋白的表达变化.结果:RT-PCR检测显示MDA-MB-231细胞中COX-2 mRNA的表达随着Celecoxib浓度的增加而逐渐降低.Celecoxib能够显著地抑制MDA-MB-231细胞增殖(P<0.05),但Celecoxib联合PGE2与单独应用Celecoxib对细胞增殖的影响差异无统计学意义(P>0.05).流式细胞术结果显示Celecoxib可使MDA-MB-231细胞阻滞在G0/G1期,并可诱发细胞凋亡.Western blot检测发现Celecoxib作用MDA-MB-231细胞24 h,随着Celecoxib浓度的增加,Caspase-3蛋白表达增加,P65蛋白表达下调.结论:Celecoxib可以通过下调P65蛋白的表达从而阻断NF-kB信号途径,抑制人乳腺癌MDA-MB-231细胞增殖,促进其凋亡. 相似文献
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L-Arginine is the common substrate for two enzymes, arginase and nitric oxide synthase (NOS). Arginase converts L-arginine to L-ornithine, which is the precursor of polyamines, which are essential components of cell proliferation. NOS converts L-arginine to produce NO, which inhibits proliferation of many cell lines. Various human breast cancer cell lines were initially screened for the presence of arginase and NOS. Two cell lines, BT-474 and MDA-MB-468, were found to have relatively high arginase activity and very low NOS activity. Another cell line, ZR-75-30, had the highest NOS activity and comparatively low arginase activity. The basal proliferation rates of MDA-MB-468 and BT-474 were found to be higher than the ZR-75-30 cell line. N-Hydroxy-L-arginine (NOHA), a stable intermediate product formed during conversion of L-arginine to NO, inhibited proliferation of the high arginase-expressing MDA-MB-468 cells and induced apoptosis after 48 h. NOHA arrested these cells in the S phase, increased the expression of p21, and reduced spermine content. These effects of NOHA were not observed in the ZR-75-30 cell line, which expresses high NOS and relatively low arginase. The effects of NOHA were antagonized in the presence of L-ornithine (500 microM), which suggests that in MDA-MB-468 cell line, the arginase pathway is very important for cell proliferation. Inhibition of the arginase pathway led to depletion of intracellular spermine and apoptosis as observed by terminal deoxynucleotidyl transferase (Tdt)-mediated nick end labeling assay and induction of caspase 3. In contrast, the ZR-75-30 cell line maintained its viability and its L-ornithine and spermine levels in the presence of NOHA. We conclude that NOHA has antiproliferative and apoptotic actions on arginase-expressing human breast cancer cells that are independent of NO. 相似文献
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Miglietta A Bozzo F Bocca C Gabriel L Trombetta A Belotti S Canuto RA 《Cancer letters》2006,234(2):149-157
We investigated the molecular mechanisms involved in the anti-proliferative activity exerted by conjugated linoleic acid (CLA) on the estrogen unresponsive MDA-MB-231 human breast cancer cell line. The effects on cell proliferation, cell cycle progression and induction of apoptosis were examined. CLA caused the reduction of cell proliferation along with the accumulation of cells in the S phase of the cycle. The occurrence of apoptosis in these cells was indicated by flow cytometry data and further confirmed by the onset of cells with morphological features typical of apoptosis. ERK1/2 reduction and upregulation of pro-apoptotic protein Bak were induced. These events were associated with: (a) reduced levels of the anti-apoptotic protein Bcl-x(L), (b) the translocation of cytochrome c from the mitochondria to the cytosol, (c) the cleavage of pro-caspase-9 and pro-caspase-3. From the above data, we are induced to think that CLA may trigger apoptosis in the estrogen unresponsive MDA-MB-231 cell line via mechanisms involving above all the mitochondrial pathway. 相似文献
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目的 探讨盐霉素(salinomycin,Sal)对三阴性乳腺癌细胞MDA-MB-231增殖和凋亡的影响及其作用机制。方法 采用流式细胞术筛选ALDH高表达乳腺癌细胞系;采用MTT法检测不同浓度Sal(1~32 μmol/L)对细胞增殖的影响;采用流式细胞仪检测Sal对细胞凋亡以及肿瘤干细胞表面标志物ALDH表达的影响;采用qRT-PCR、Western blot检测凋亡相关分子Caspase-3、Bax、Bcl-2、PARP mRNA和蛋白表达;TCGA(the Cancer Genome Atlas)数据库下载乳腺癌患者数据集(n=1 218),采用Pearson法分析ALDH与凋亡相关分子表达的相关性。结果 Sal在24 h、48 h、72 h均可以剂量依赖的方式抑制MDA-MB-231细胞增殖(均P<0.001);Sal作用MDA-MB-231细胞48 h后,与对照组比较,2 μmol/L组、4 μmol/L组、8 μmol/L组的细胞凋亡率均升高(均P<0.05);Bcl-2、PARP、Caspase-3 mRNA表达下降(均P<0.05),Bax mRNA表达升高(均P<0.05);Bcl-2、Caspase-3 蛋白表达下降(均P<0.05),PARP、Bax 蛋白表达升高(均P<0.05);MDA-MB-231乳腺癌干细胞表面标志物ALDH表达下降(均P<0.05)。TCGA数据库分析显示,ALDH与抗凋亡分子Bcl-2、Caspase-3的表达呈正相关(r=0.209,P=0.007;r=0.235,P=0.002),与Bax、PARP的表达呈负相关(r=-0.326,P<0.001;r=-0.453,P<0.001)。结论 Sal可抑制人乳腺癌MDA-MB-231细胞生长,可能通过下调肿瘤干细胞表面标志物ALDH表达实现。 相似文献
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目的:应用小干扰RNA(small interfering RNA, siRNA)乳腺癌MDA-MB-231细胞中真核细胞翻译起始因子4E (eukaryotic translation initiation factor 4E, EIF 4E )基因的表达,探讨其对乳腺癌细胞增殖和生长周期的影响。方法:构建针对EIF 4E 基因的siRNA表达质粒,采用脂质体法将表达质粒pGPU6/GFP/Neo-EIF4E转染人乳腺癌MDA-MB-231细胞;分别采用RT-PCR、Western 印迹法和免疫细胞化学法检测转染EIF4E siRNA后, 对MDA-MB-231细胞中EIF4E及cyclinD1 mRNA和蛋白的表达水平的影响;MTT法、平板克隆形成实验和FCM检测MDA-MB-231细胞增殖活性、克隆形成率及细胞周期。结果:成功构建EIF4E siRNA表达质粒。RT-PCR、Western 印迹法和免疫细胞化学法检测结果显示,MDA-MB-231细胞中EIF4E及cyclinD1 mRNA和蛋白表达均明显下降(P <0.05)。转染EIF4E siRNA组细胞生长缓慢,集落形成数明显减少,与空白对照组和转染空载体组比较,差异有统计学意义(P<0.05)。FCM检测结果显示,EIF4E siRNA转染组G1期细胞所占百分比为(71.30±0.47)%, 较空白对照组的( 53.10±0.43)%明显增加,而S期细胞所占百分比为(12.53±0.13)%,较空白对照组的(26.47±0.38)%明显减少(P<0.05)。结论:EIF4E siRNA可显著下调EIF4E基因在乳腺癌MDA-MB-231细胞中的表达水平,在一定程度上抑制乳腺癌细胞的增殖,有可能成为临床治疗乳腺癌的靶点之一。 相似文献
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目的:研究乳腺癌肿瘤相关成纤维细胞(carcinoma-associated fibroblasts,CAFs)对乳腺癌细胞增殖、凋亡、黏附和侵袭能力的影响.方法:首先,从6例乳腺癌病人肿瘤及癌旁正常乳腺组织块分别分离培养成对的CAFs和正常成纤维细胞(normal fibroblasts,NFs).然后,通过Transwell小室将CAFs或NFs与乳腺癌MDA-MB-231细胞体外共培养,进行增殖(MTT)、侵袭、黏附实验及流式细胞仪细胞凋亡检测.结果:MDA-MB-231与CAFs或NFs共培养后乳腺癌细胞的增殖活性显著增强(P<0.05).与NFs相比,这种差异在同CAFs共培养组更为明显(P =0.011).与CAFs共培养之后,MDA-MB-231的早期凋亡显著减少(P=0.026);而与NFs共培养之后则无显著差异.CAFs或NFs对MDA-MB-231细胞的细胞周期和晚期凋亡无明显影响.与CAFs或NFs共培养24小时后,MDA-MB-231细胞的侵袭、黏附能力明显增强,黏附数目为(34.7±4.84)个/视野(CAFs)和(20.16±0.09)个/视野(NFs),(P =0.000);侵袭数为(89±4.62)个/视野(CAFs)和(81.6±6.08)个/视野(NFs),(P<0.05).结论:CAFs能促进乳腺癌MDA-MB-231细胞的增殖、减少其早期凋亡;同时对乳腺癌细胞的黏附和侵袭能力有促进作用.可是NFs的作用较CAFs为弱.有关CAFs影响乳腺癌肿瘤细胞的生物学特性的机制有待于进一步研究. 相似文献