c—erbB—2,c—erbB—4原癌基因蛋白表达与乳腺癌妆生的相关关系

目的 研究ｃ－ｅｒｂＢ－２、ｃ－ｅｒｂＢ－４原癌基因过度表达与乳腺癌发生及其病理参数的相关关系。方法 对８０例乳腺癌、１６例乳腺纤维瘤、９例乳腺纤维囊性病、１７例乳腺癌转移淋巴垄石蜡切块采用免疫组化（ＬＳＡＢ法）结合计算机图像分析研究原癌基因产物ｃ－ｅｒｂＢ－２、ｃ－ｅｒｂＢ－４的表达情况。结果 Ｃ－ｅｒｂＢ－２在乳腺癌中过度表达有显著意义。ｅ－ｅｒｂＢ－２在乳腺癌转移洒巴结中的表达较原发灶中高，     

Relationship between changes of N—methyl—D—aspartate receptor activity and brain after brain injury in rats

OBJECTIVE: To investigate the relationship between the changes of N-methyl-D aspartate (NMDA) receptor activity and brain edema after injury in rats. METHODS: The brain injury models were made by using a free-falling body. The treatment model was induced by means of injecting AP5 into lateral ventricle before brain injury; water contents in brain cortex were measured with dry-wet method; and NMDA receptor activity was detected with a radio ligand binding assay. RESULTS: The water contents began to increase at 30 minutes and reached the peak at 6 hours after brain injury. The maximal binding (B(max)) of NMDA receptor increased significantly at 15 minutes and reached the peak at 30 minutes, then decreased gradually and had the lowest value 6 hours after brain injury. Followed the treatment with AP5, NMDA receptor activity in the injured brain showed a normal value; and the water contents were lower than that of AP5-free injury group 24 hours after brain injury. CONCLUSIONS: It suggests that excessive activation of NMDA receptor may be one of the most important factors to induce the secondary cerebral impairments, and AP5 may protect the brain from edema after brain injury.     

联合应用c—Ha—ras,c—myc反义寡聚脱氧核苷酸对人膀胱癌细胞生长增殖…

作者联合应用人工合成的ｃ－Ｈａ－ｒａｓ，ｃ－ｍｙｃ反义寡聚脱氧核苷酸（ＡＳＯ－ｒ、ＡＳＯ－ｍ）对人膀胱癌细胞株进行作用，观察到ＡＳＯ－ｒ、ＡＳＯ－ｍ能明显抑制癌细胞中ｒａｓＰ２１、ｍｙｃＰ６２的表达，抑制了癌细胞ＤＮＡ合成及生长增殖，经ＡＳＯ－ｒ、ＡＳＯ－ｍ处理后癌细胞裸鼠致瘤力下降，这一结果表明：针对多外癌基因联合应有反义寡聚脱氧核苷酸，可能给膀胱癌的治疗提供新的途径，有进一步探索的价值。     

Eeffects of nerve growth factor on N—methyl—D—asparate receptor 1 after spinal cord injury in rats

OBJECTIVE: To explore the effects of the nerve growth factor (NGF) on N-methyl-D-asparate receptor 1 (NMDAR 1) after spinal cord injury. METHODS: Spinal cord injury of Wistar rats was performed with Allen's method by a 10 gx2.5 cm impact on the posterior T8 spinal cord. NGF was given to the rats of the treatment group via subarachnoid space tube at once, 2, 4, 8, 12 and 24 hours after spinal cord injury, respectively. The expression of NMDAR1 mRNA in spinal cord was detected by in situ hybridization. RESULTS: Rare expression sequence of NMDAR1 mRNA was found in rat spinal cord of the normal group. A strong expression sequence of NMDAR1 mRNA was found in rat spinal cord of the normal saline group. The expression of NMDAR1 mRNA in the NGF group was significantly decreased as compared with that in the normal saline group (P=0.01). CONCLUSIONS: NGF can relieve damage of injured spinal cord by prohibiting the expression of NMDAR1 mRNA.     

胆—肠Roux—Y吻合术的长期效果

胆－肠Ｒｏｕｘ－Ｙ吻合术的长期效果林擎天王洪胆－肠Ｒｏｕｘ－Ｙ吻合术是胆道外科常用的一种内引流手术。它的适应症较广，可用于良性或恶性胆道疾病。现为总结其长期效果，本文仅就我院１５年来对４７例良性胆道疾病使用的情况报导如下：临床资料１一般资料：全组４７...     

C—erbB—2和波形蛋白在乳癌表达及临床意义分析

Ｃ－ｅｒｂＢ－２和波形蛋白在乳癌表达及临床意义分析辽宁省人民医院病理科（１１００１５）景士兵魏少忠任秋华王泽兴中国医科大学第二临床学院病理科（１１０００３）何小明沈阳市红十字会医院外科（１１００４３）吴星恺近年来研究报告表明Ｃ－ｅｒｂＢ－２在乳癌中的...     

反转录—多聚酶链反应扩增小鼠睾丸17α—羟化酶mRNA

１７α－羟化酶催化雄激素合成的中间步骤，其研究于了解睾丸间质细胞内雄激素的合成具有重要的意义。本研究经二次沉淀提取小鼠睾丸组织ＲＮＡ，经反转录合成ｃＤＮＡ，以１７α－羟化酶ｃＤＮＡ特异序列引物进行ＰＣＲ扩增。结果显示，二次沉淀后用ＲＴ－ＰＣＲ方法能获得一的１７α－羟化酶特异中段。     

N—Acety1 β—D—glucosaminidase is not attached to human sperm membranes through the glycosylphosphatidyl inositol（GPI）—anchor

AIM: The mode of anchorage of N-acetyl beta-D-glucosaminidase (NAGA) on human ejaculated sperm was investigated. METHODS: Sperm plasma membrane was prepared by discontinuous sucrose gradient centrifugation from human sperm. NAGA was solubilized from these membranes by two detergents: octyl-glycoside and triton X-100. In separate studies, the release of the enzyme from the sperm membrane preparation by phosphatidylinositol specific phospholipase C (PI-PLC) was also examined. RESULTS: NAGA activity was detected on sperm membranes isolated from human ejaculates. The pattern of the enzyme solubilization by detergents indicated that the enzyme was an integral protein of sperm membrane. NAGA was not released from the sperm membranes by PI-PLC treatment. CONCLUSION: The evidence presented strongly suggests that human sperm membrane bound NAGA is not attached via the GPI anchor.     

IL—1,TNF和IL—6与骨关节炎

骨关节炎患者关节滑液中可检测了高水平的ＩＬ－１，ＴＮＦ和ＩＬ－６，这是参与滑膜炎性病变，关节软骨基质降解及干扰软骨细胞功能的重要细胞因子，对其采取有效的抑制措施可能是早期防治骨关节炎的新途径。     

三维多孔骨修复材料DL—PLA及β—TCP／DL—PLA的体外降解研究

目的：比较DL-PLA、20%β-TCP/DL-PLA制成的三维多孔材料降解特点，选择有利于骨缺损修复的材料。方法：应用浇 铸盐析技术分别将DL-PLA、20%β-TCP/DL-PLA制成三维多孔结构，通过体外降解实验观察测量并对比两种材料的分子量、重量、生物力学、pH值的动态变化。结果：在降解过程中DL-PLA材料pH值在3周内轻度下降，而后明显下降，12周时仅为2.94。20%β-TCP/DL-PLA材料在12周的降解过程中pH值十分稳定，12周时为6.6。20%β-TCP/DL-PLA在0-2周分子量变化较大，从29400下降为28200，2周后下降较平缓，12周时为12000，而DL-PLA在0-2周分子量变化极大，从39400下降为10900，12周时为2049。20%β-TCP/DL-PLA重量变化率0-4周时变化较小，4周后下降加快，12周时为初始重量的60%。DL-PLA与之相似12周时为初始重量的55%。初始强度20%β-TCP/DL-PLA为1.72MPa，DL-PLA为1.35MPa，两者有显著差别（P＜0.05)DL-PLA在降解过程中DL-PLA组0-4周下降极小，4-6周下降较快，12周为0.13MPa，20%β-TCP/DL-PLA在0-6周下降极小，6-8周下降较快，12周时为0.22MPa。结论：三维多孔20%β-TCP/DL-PLA作为一种新型治疗骨缺损的材料，降解特点有利于骨组织细胞长入，降解化学环境有利于骨组织细胞生长。     

上皮组织抗菌肽—β—防御素

新近发现的β－防御素（HBD）家族，广泛存在于皮肤和粘膜上皮组织中，其功能的失活与病源微生物定植、集落于皮肤和粘膜上皮而导致严重感染关系密切。目前已发现3种HBD：HBD－1、HBD－2和HD－3。HBD家族具有广谱高效抗菌作用，迄今尚未发现有致病菌株对其有耐药性。     

大肠癌组织胃泌素与c—myc,c—fos表达的关系

目的 研究大肠癌组织中胃泌素与癌基因ｃ－ｍｙｃ、ｃ－ｆｏｓ表达的关系,以探讨胃泌素对大肠癌的促增殖作用机理。方法 采用免疫组化ＳＰ法检测４８例大肠癌患者癌组织及癌旁粘膜胃泌素和癌基因ｃ－ｍｙｃ、ｃ－ｆｏｓ的表达。结果 大肠癌组织胃泌素阳性率为３９．５８％,高分化腺癌明显高于低分化和粘液腺癌（Ｐ〈０．０５）。癌组织的ｃ－ｍｙｃ和ｃ－ｆｏｓ表达阳性率显著高于癌旁粘膜和正常粘膜。胃泌素阳性组癌组织ｃ－ｍｙｃ和ｃ－ｆｏｓ表达阳性率分别为７８．９４％和７３．６８％,胃泌素阴性组分别为３７．９３％和３１．０４％,差异均有显著性意义（Ｐ〈０．０５,Ｐ〈０．０１）。结论 部分大肠癌细胞通过自分泌方式产生胃泌素,可能通过增加ｃ－ｍｙｃ、ｃ－ｆｏｓ等癌基因的表达,从而刺激癌细胞的价值。     

胰腺癌细胞对5—Fu和吉西它滨获得性耐药后bcl—XL和mcl—l表达的变化

目的：探讨胰腺癌细胞对5－Fu和吉西它滨的获得性耐药后bcl-XL和mcl-l表达的变化。方法：应用SRB方法检测5－Fu和吉西它滨对胰腺癌细胞株Panc-1,Mia-Paca-2和Capan-1的细胞毒性作用，应用Western blot法来检测bcl-XL和mcl-l的蛋白表达水平。结果：5－Fu和吉西它滨对3株胰腺癌细胞均产生了细胞毒性作用，5－Fu长期作用后，Capan-1细胞IC50上升了2．1倍（P＜0．05）。吉西它滨长期作用后，Capan-1细胞IC50上升了1．5倍（P＜0．05）。5－Fu或吉西它滨长期作用后，产生了获得性耐药的胰腺癌细胞bcl-XL和mcl-1表达水平上调。结论：5－Fu或吉西它滨长期作用后，产生了获得性耐药，这种耐药与bxl-XL和mcl-l的过度表达相关。     

消化道肿瘤手术前后sIL—2R和sTNF—RI的临床研究

目的：探讨消化道肿瘤手术前后ｓＩＬ－２Ｒ和ｓＴＮＦ－ＲＩ在血清中的浓度。方法：用双抗体夹心（ＥＬＩＳＡ）法测定８０例肿瘤患者（胃癌、大肠癌及少部分肝癌），２６例非肿瘤患者（胆囊结石、良性胃溃疡、乳房纤维瘤、结肠息肉等），６１例志愿健康成年人血清ｓＩＬ－２Ｒ及ｓＴＮＦ－ＲＩ水平明显高于良性疾病患者和健康人对照组（Ｐ〈０．０１），按肿瘤ＴＮＭ分期，Ⅲ－Ⅳ期患者远高于Ⅰ－Ⅱ期患者（Ｐ〈０．０１），而与病     

移植肾组织白细胞介素—10和干扰素—γ的基因表达

目的：研究移植肾组织内细胞因子的基因表达与免疫耐受及排斥反应的关系。方法：SD和Wistar大鼠各40只，受体随机分4组移植，正常对照组，移植过程腔静脉输注供体脾细胞组、单用环孢素A（CsA)组CsA加体脾细胞腔静脉注射组各10只。逆转录－聚合酶链反应（RT－PCR）技术测定移植后肾组织内白细胞介素（IL）－10和干扰素（INF）γmRNA的表达，结果：IL－10mRNA的表达（P＜0．01）在耐受组（16例中14例阳性）明显高于排斥组（14例中4例阳性，LNF－γmRNA的表达（P＜0．01），在排斥组（14例中10例阳性）明显高于耐受组（16例中1例阳性）。结果：RT－PCR检测移植肾内IL－10和INF－γ的mRNA的表达，是一种敏感性高的检测方法，前者提示免疫耐受状态，后者提示排斥反应。