Caspase-3在皮肤鳞状细胞癌及光线性角化病中的表达

目的：检测Caspase-3在皮肤鳞状细胞癌及光线性角化病组织中的表达。方法： 应用免疫组化法检测16例皮肤鳞状细胞癌皮损、27例光线性角化病皮损及24例正常皮肤组织中Caspase-3蛋白的表达。结果：Caspase-3在皮肤鳞状细胞癌、光线性角化病及正常皮肤组织的表达率分别为37.50%,51.85%,79.17%,其表达含量在皮肤鳞状细胞癌、光线性角化病、正常皮肤组织逐渐增加。结论：Caspase-3蛋白表达下调可能参与皮肤鳞状细胞癌及光线性角化病的发病过程。     

下肢象皮肿溃疡继发鳞状细胞癌

报告1例下肢象皮肿溃疡继发鳞状细胞癌。患者女,65岁。双下肢肿胀、皮肤增厚60年,左踝出现溃疡21年。溃疡处皮肤组织病理检查：真皮中可见大量鳞状细胞团块,细胞有异形性,核深染,见核分裂相,可见瘤巨细胞。免疫组化染色：CK（＋＋＋）。诊断为皮肤鳞状细胞癌。     

鳞状细胞癌组织中HHV-8 K15等位基因型研究

目的 探讨皮肤鳞状细胞癌和食道鳞状细胞癌患者癌组织中人类疱疹病毒8型（HHV-8）K15等位基因型与肿瘤的发病关系。方法 序列特异性引物-巢式PCR技术检测40例皮肤鳞状细胞癌、40例食道鳞状细胞癌石蜡包埋组织中HHV-8 K15基因,并确定HHV-8 K15等位基因型,运用χ2检验对结果进行统计学分析。结果 9例皮肤鳞状细胞癌（22.5%）石蜡包埋组织中检测到HHV- 8 K15P型,8例食道鳞状细胞癌（20%）石蜡包埋组织中检测到 HHV- 8K15P型,两种肿瘤均未检测出K15M基因型。HHV-8在皮肤鳞状细胞癌与食道鳞状细胞癌感染率组间比较差异无统计学意义（P > 0.05）。结论 鳞状细胞癌中感染HHV-8K15P基因为K15P等位基因型,其发生发展可能与HHV-8的感染有一定的关系。     

p53、Gadd45α蛋白在皮肤鳞状细胞癌、基底细胞癌中的表达

目的:检测p53和Gadd45α蛋白在皮肤鳞状细胞癌和基底细胞癌组织中的表达.方法:应用免疫组化法对30例皮肤鳞状细胞癌和25例基底细胞癌组织中的p53和Gadd45α蛋白表达进行检测.结果:p53蛋白在皮肤鳞状细胞癌和基底细胞癌组织中的表达阳性率分别为56.67%、48%,与正常皮肤组织比较差异均有显著性(P=0.006;0.025);Gadd45α在皮肤鳞状细胞癌和基底细胞癌中表达分别为43.33%、52%,均高于正常皮肤组织的表达(P=0.031;0.010).高分化皮肤鳞状细胞癌组阳性表达率为75%,高于中低分化组的22.22%(P=0.008).结论:皮肤鳞状细胞癌和基底细胞癌组织中p53、Gadd45α异常表达可能参与了发病过程.     

光动力疗法治疗皮肤鳞状上皮细胞癌7例疗效观察

目的观察5-氨基酮戊酸光动力疗法(ALA-PDT)治疗皮肤鳞状细胞癌的临床疗效和复发率。方法对7例经组织病理学确诊的皮肤鳞状细胞癌进行(ALA-PDT治疗。结果经(3~6)次治疗后,7例患者溃疡面均被肉芽组织覆盖后愈合。随访6月后,均未发现原皮损处复发,2例患者治疗部位有轻度刺痛、瘙痒。结论对皮肤鳞状细胞癌尤其老年患者,ALA-PDT疗法损伤小、美容效果好。     

皮肤鳞状细胞癌13例临床诊治体会

目的探讨皮肤鳞状细胞癌的临床表现,组织病理及诊治。方法对13例临床拟诊为皮肤鳞状细胞癌的患者,组织病检确诊后,采取外科手术,高频电离子治疗。结果 13例患者均经组织病理检查证实为皮肤鳞癌,其中2例放弃治疗,2例行高频电离子治疗,9例行手术治疗。对治疗后的11例患者分别随访1月～16年,患者均健在,病灶无转移。结论鳞状细胞癌是一种恶性肿瘤,早期发现,早期诊断,早期治疗是提高生存率的关键。     

Livin和Caspase-3在皮肤基底细胞癌和皮肤鳞状细胞癌组织中的表达及相关性分析

目的了解皮肤基底细胞癌和皮肤鳞状细胞癌组织中Livin和Caspase-3的表达情况及二者的关系。方法采用免疫组化法检测25例基底细胞癌、18例鳞状细胞癌组织中Livin和Caspase-3的表达情况。结果①Livin蛋白在正常皮肤组织中不表达,基底细胞癌和鳞状细胞癌组织中Livin蛋白的阳性表达率分别为64.00%和72.22%。②Caspase-3在正常皮肤组织中表达,且明显高于基底细胞癌和鳞状细胞癌组织中Caspase-3的表达。③Caspase-3的表达与Livin的表达呈显著负相关。结论Livin在基底细胞癌和鳞状细胞癌组织中高表达,Caspase-3蛋白在基底细胞癌和鳞状细胞癌组织中低表达,且与Livn的表达呈负相关,提示二者可能共同参与基底细胞癌和鳞状细胞癌发病过程。     

基底细胞癌和鳞状细胞癌中转化生长因子βⅠ型和Ⅱ型受体表达

目的探讨转化生长因子β(TGFβ)受体在基底细胞癌和鳞状细胞癌中表达水平的变化及其意义。方法采用实时定量PCR和SP免疫组化技术分别检测基底细胞癌、鳞状细胞癌及正常人对照皮肤中Ⅰ型和Ⅱ型TGFβ受体(TGFβRⅠ,TGFβRⅡ)mRNA和蛋白的表达。结果对18例基底细胞癌、24例鳞状细胞癌患者的皮损及正常人对照皮肤的研究显示,基底细胞癌和鳞状细胞癌皮损TGFβRⅠ和TGFβRⅡ的mRNA表达水平均显著低于正常人对照皮肤。免疫组化试验结果显示;TGFβRⅠ染色强度在基底细胞癌组、鳞状细胞癌组较正常人对照皮肤组显著降低(P<0.001);TGFβRⅡ在二组表皮肿瘤与正常人对照皮肤组问表达差异也有统计学意义(P<0.01)。结论基底细胞癌和鳞状细胞癌中TGFβ受体表达下调可能有助于这些上皮细胞起源的表皮肿瘤的形成。     

神经生长因子及受体在非黑素性皮肤癌中的表达

目的：探讨神经生长因子（NGF）及受体P75在非黑素性皮肤癌中的表达情况。方法：用原位杂交的方法对15例鳞状细胞癌（SCC）、12例基底细胞癌（BCC）、13例正常上皮（NS）中NGF及受体P75 mRNA进行了检测。结果：与正常皮肤组织比较,NGF mRNA在基底细胞癌组织中的表达明显增高（t=30.06,P〈0.01）,在鳞状细胞癌组织中的表达明显下降（t=19.62,P〈0.01）,鳞状细胞癌组织与基底细胞癌组织相比差异有统计学意义（t=57.36,P〈0.01）。P75mRNA在皮肤正常组、基底细胞癌组、鳞状细胞癌组中的表达呈逐渐降低趋势,组与组之间两两比较差异均有统计学意义：鳞状细胞癌组与正常皮肤组（t=42.69,P〈0.01）,基底细胞癌组与正常皮肤组（t=30.37,P〈0.05）,鳞状细胞癌组与基底细胞癌组（t=18.49,P〈0.05）。结论：NGF及受体P75可能在非黑素性皮肤癌发生及浸润中起一定作用。     

Survivin和COX-2在皮肤基底细胞癌和鳞状细胞癌组织中的表达及相关性

目的了解皮肤基底细胞癌和皮肤鳞状细胞癌中Survivin和COX-2的表达情况及两者的关系。方法采用免疫组化法检测10例正常对照组、23例基底细胞癌、18例鳞状细胞癌组织中Survivin和COX-2的表达情况。结果 Survivin蛋白在正常组织中不表达,基底细胞癌和鳞状细胞癌中Survivin蛋白的表达率分别为60.87%和66.67%。COX-2在正常组织中的表达率为10%,基底细胞癌和鳞状细胞癌中COX-2的表达率分别为65.22%和66.67%,且明显高于其在正常组织中的表达率。Survivin的表达和COX-2的表达呈显著正相关(P0.05)。结论 Survivin蛋白和COX-2在皮肤基底细胞癌和皮肤鳞状细胞癌中高表达,两者呈正相关。     

40例寻常型银屑病患者血清锌与超氧化物歧化酶检测

目的:观察血清锌与超氧化物歧化酶(SOD)对寻常型银屑病的影响,探讨银屑病的病因和发病机制。方法:对40例银屑病患者各期行PASI评分;银屑病患者各期和对照组采用原子分光光度法检测血清锌值,用黄嘌呤氧化酶法检测SOD。结果:银屑病患者各期血清锌和SOD较对照组显著降低(P<0.01);银屑病患者各期SOD与PASI评分呈负相关(P<0.01),血清锌与SOD呈负相关(P<0.01),但对照组血清锌与SOD呈负相关(P<0.01)。结论:银屑病患者存在着血清锌和SOD的降低;缺锌是银屑病患者SOD降低的主要原因。     

Element concentrations in serum, erythrocytes, hair and urine of alopecia patients

The trace element concentrations of Se, Rb, Zn, Fe, Co, Cs, Mg, Ca, F, Cu, Cr and Ag in serum and of Se, Rb, Zn, Fe, Co and Cs in red cells of Finnish alopecia patients were determined. In addition the Cu and Zn content in 24 h urine and Cu, Zn, Cd, Cr and Se concentrations in the hair of these patients were studied. No differences in element concentrations of the samples mentioned above as compared to those of the normal population could be found. In addition, there was no tendency of excesses or deficiences of elements analysed in the samples. Statistically significant difference was found between the copper content of serum in alopecia areata and alopecia universalis patients and also between the copper content of serum in alopecia areata plus alopecia totalis and alopecia universalis patients. The selenium concentration in serum samples of a few patients was low, but this is in agreement with the fact that the selenium content in the Finnish population is low due to the scanty content of selenium in food.     

白癜风患者血清及皮肤组织液铜锌含量测定

本文用感应藕合等离子发射光谱仪,测定了27例白癜风患者和36例健康人血清中铜锌两种微量元素的含量,并对其中24例白癜风患者无病变部位和白斑部位的皮肤吸力水疱疱液进行了测定。结果显示白癜风患者血清中铜锌的含量均低于正常对照组,病人白斑部位组织液铜含量明显低于无病变部位,提示白癜风发病与铜锌两种微量元素有关。     

寻常型银屑病皮损HIF-1α和血清IGF-Ⅱ水平的检测

目的：探讨缺氧诱导因子-1α（HIF-1α）和胰岛素样生长因子-II（IGF-II）在寻常型进行期银屑病发病中的意义.方法：对42例寻常型进行期银屑病患者中的20例取其皮损进行免疫组化法,检测其HIF-1α的表达和分布,同时取20例健康皮肤组织作对照;对另外22例患者的血清标本采用ELISA法,检测其IGF-II浓度,并取健康血清20例作对照.结果：皮损组HIF-1α表达阳性细胞数（25.21±24.45）明显高于对照组（3.80±7.27）（P〈0.05）,两组间阳性率差异有统计学意义;患者血清IGF-II水平（308.39±432.74）明显高于正常对照组（106.73±23.23）（P〈0.05）,两组间具有统计学差异.结论：患者皮损中HIF-1α的表达和血清IGF-II的水平均较正常水平明显增高,二者可能参与银屑病的发病过程.     

Foxpl及Ki-67在皮肤基底细胞癌的表达

目的：检测皮肤基底细胞癌（BCC）组织中Foxpl和Ki一67蛋白的表达。方法：采用免疫组化方法检测BCC组织石蜡切片中Foxpl和Ki-67蛋白的表达。结果：40例BCC标本中Foxpl蛋白的表达率为82．5％,Ki-67蛋白的表达率为75％,均显著高于对照组（均P〈O．01）。结论：Foxpl和Ki-67可能参与BCC的发生和发展。     

儿童寻常性银屑病患者血清锌水平检测

目的探讨血清锌(Zn)的变化与儿童银屑病发病的关系.方法采用原子吸收分光光度法(AAS)检测血清Zn的水平.结果银屑病患儿组血清Zn水平明显低于正常对照组,差异有极显著性,且进行期血清中Zn水平较静止期低,差异有显著性.银屑病患儿血清Zn与PASI评分之间成负相关关系.结论儿童银屑病患者体内存在微量元素Zn的代谢紊乱,Zn可能与儿童银屑病的发病有关.     

白癜风患者血清及皮损中某些酶及微量元素改变研究

本研究检测白癜风患者血清中超氧化物岐化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、黄嘌呤氧化酶(XOD)活性,丙二醛(MDA)水平及血清、皮肤中锌(Zn)、铜(Cu)、硒(Se)、铁(Fe)含量。结果表明:患者血清中GSH-Px、SOD活性及Cu含量低于对照组(P<0.01),MDA、Fe含量高于对照组(P<0.01).皮损处Zn、Cu、Se或Fe含量分别低于或高于周围外观正常皮肤,治疗后血清SOD、GSH-Px活性增高、Cu、Fe含量显著降低(P<0.01)。提示:白癜风患者自由基防御系统中部分酶活性降低,Zn、Cu、Se缺乏。     

Serum and tissue CTACK/CCL27 chemokine levels in early mycosis fungoides may be correlated with disease-free survival following treatment with interferon alfa and psoralen plus ultraviolet A therapy

Background Neoplastic T‐cell recruitment into the skin is a critical step in the pathogenesis of mycosis fungoides (MF), and the cutaneous T‐cell attracting chemokine, CTACK/CCL27, might be involved. Objectives To investigate the clinical and prognostic significance of CTACK/CCL27 levels in patients with early‐stage MF. Methods Serum samples and skin biopsy specimens were collected from 15 patients at the time of diagnosis and after the end of treatment with psoralen plus ultraviolet A/interferon alfa‐2b combination therapy. Serum samples were also collected from 20 healthy donors as controls. CTACK/CCL27 serum levels were analysed by enzyme‐linked immunosorbent assays. CTACK/CCL27 tissue expression was determined by immunohistochemistry on skin biopsy specimens taken at diagnosis and after therapy. Event‐free survival was taken as the primary clinical outcome. Results In patients with MF at diagnosis, CTACK/CCL27 serum levels were not significantly different from healthy controls, whereas CTACK/CCL27 expression in the skin was increased in 87% of cases compared with normal controls. After therapy, all patients obtained a clinical complete remission, serum levels did not change significantly and tissue expression remained abnormal in 80% of patients, even if complete histological remission was recorded. Serum levels were not significantly different in cases with different intensity of cutaneous immunostaining. Eight patients experienced a relapse: the combination of high CTACK/CCL27 levels both in sera and skin increased the probability of experiencing an event at 51 months from 36% to 83%. Conclusions Our data seem to indicate that CTACK/CCL27 levels in skin and sera after therapy might be correlated with risk of recurrence.     

Evaluation of trace elements, calcium, and magnesium levels in the plasma and erythrocytes of patients with essential hyperhidrosis

Background Essential hyperhidrosis is a disease that expresses itself with excessive sweating in palmar, plantar, axillary, and craniofacial regions. The etiopathogenesis of the disease, which has particular importance because of leading to psychosocial morbidity, could have not been completely elucidated. In previous studies, it has been shown that oxidative stress might play a role in the pathogenesis. Aims Assessing the levels of trace elements such as Se, Zn, Cu, Fe, and Mg that have an important role in oxidative stress, as well as Ca and Mg that have an important role in membrane physiology, in patients with essential hyperhidrosis. Materials and methods Blood samples taken from the patient group with essential hyperhidrosis (42) and the control group (37) were separated into plasma and erythrocytes, and the levels of the bioelements were measured by use of ICP‐OES device. Results Erythrocyte levels of Se, Fe, Cu, Zn, Ca, and Mg were detected significantly higher in patients with essential hyperhidrosis. Furthermore, plasma levels of Cu, Ca, and Mg were significantly lower in patients with essential hyperhidrosis. Plasma levels of Se, Fe, and Zn showed no statistical difference between two groups. Dicussion It was thought that the high levels of Cu and Fe in erythrocytes may play a role in increased intracellular oxidative stress, whereas the increase in Se and Zn levels may be secondary to increased oxidative stress. Low extracellular concentrations of Ca and Mg raise the thought that they play a role either enhancing the membrane excitability of eccrine sweat glands or influencing the autonomic nerve system. Conclusion The levels of trace elements, which were determined to be different from the control group, may play a role in the pathogenesis of essential hyperhidrosis either in direct relation with or without oxidative mechanisms.     

Analyses of Serum Copper and Zinc Levels and Copper/Zinc Ratios in Skin Diseases

With the objective of comprehending abnormal metabolisms of the essential metals of zinc (Zn) and copper (Cu) in three groups of skin diseases, skin cancer, inflammatory diseases, and non-inflammatory disease, we measured serum levels of Zn and Cu in 151 cases of various cutaneous manifestations and estimated the significance of the ratios between the two metals (Cu/Zn). The serum level of Zn was significantly decreased in cases of bullous pemphigoid, decubitus ulcer, and alopecia areata. The serum level of Cu was elevated in cases of psoriasis, decubitus ulcer, and skin cancer. We observed no elevation of serum Zn level or abnormally depressed serum Cu level. The Cu/Zn ratio showed significantly different values among these three groups of the diseases, suggesting the utility of measuring Cu/Zn ratios for differential diagnosis over that of determining the serum level of Zn or Cu alone. It was also demonstrated that, in each skin disease, the Cu/Zn ratio clearly reflects the severity of the progress.