循环免疫复合物与补体测定对SLR临床意义的比较

本文比较54例SLE患者循环免疫复合物(CIC)和补体的临床意义.肾脏受累组的CH₅₀和C₃在病情活动期较缓解期明显降低,其降低程度与病情活动程度平行;而两期的CIC均值都在正常范围内.无肾脏受累组的PEGP在病情活动期较缓解期有明显升高,升高程度与病情活动程度平行;ACA,CH₅₀和C₃则无明显意义.观察结果还提示PEGP异常升高对无肾脏受累者病情加重可能有预测作用.     

系统性红斑狼疮(SLE)患者几项免疫指标的检测及其意义的探讨

本文报道采用LTT,EtRFCT, EaRFCT和PHA皮试对SLE患者进行细胞免疫功能测定.显示有不等程度的细胞功能低下和T淋巴细胞数的减少.体液免疫功能测定显示48多病例有IgG增高,血清ANA测定阳性串为81.1%,娥识阳性率达99.3%.滴度1≥80,病情活动者较多,斑点核型最常见.周边型、斑点线型和匀质型活动性较高.狼疮性肾病出现蛋白尿者尿ANA测定41.7%阳性.在累及中枢神经系统病例70%脑脊液ANA呈阳性.血清dsDNA间接血凝法测定29.89%阳性.¹²⁵I DNA同位索法测定结果基本上与之平行,这组病例病期较短,肾累及较多,采用PEG沉淀法测定CIC有43%增高,经解离分析CIC的主要内容为核抗原和抗核抗体复合物.CH₅₀和C₃测定有不等程度降低,最后作者对SLE的发病机理进行了讨论.     

SLE患者血浆C3c检测的初步结果

本文对临床上诊断为SLE患者的血清抗dsDNA自身抗体、免疫球蛋白、补体等成分进行了测定,并介绍血浆C₃c的检测方法及初步结果.     

SLE患者衍生的EB病毒转化的B细胞产生一种经典补体途径的肾炎因子

在一些 SLE、急性感染后肾小球肾炎和 Sjg-ren综合征患者的血清中可检测到一种对C3转化酶(C4b2a)具稳定作用的经典补体途径的血清因子,称为C4肾炎因子(C4NeF),它是一种IgG类抗体。为研究SLE患者的B淋巴细胞产生C4NeF的能力,作者从15例SLE患者和10名正常健康人的外周血分离出单个核细胞,在体外用EB病毒感染,以建立有活性的B淋巴细胞系。用EAC3bBb以及EAC14b2a稳定试验测定这些已建立的细胞系上清转化经典和替代途径C3转化酶的能力。结果发     

85例白斑风患者免疫功能测定

白癜风的免疫功能测定,国内尚无报告.近3年来我们对85例白瘫风患者分别作了E玫瑰花形成试验(ERFT),淋巴细胞转化率(LTT),总补体(CH₅₀),补体第3成分(C₃),循环免疫复合物(CiC),以及IgG,IgA,IgM的检查测定,现报告如下.     

银屑病患者血清及皮肤中补体及其活化成分的变化

目的 研究银屑病患者血清及皮肤中补体及其活化成分的变化,以探讨其与银屑病皮损形成的关系。方法 采用双抗体夹心ELISA法和免疫比浊法检测了57例银屑病患者血清补体活化片段C3d及可溶性补体激活片段sC5b-9及补体C3、C4的变化;采用免疫组化法(ABC法)检测了37例银屑病患者皮损组织、非皮损组织及20例正常皮肤组织C5b-9的原位表达。结果 银屑病患者血清中C3、C4水平明显下降;C3d、sC5b-9水平明显增高,与对照组比较差异有非常显著性,进行期患者血清中C3、C4明显低于静止期,而C3d、sC5b-9明显高于静止期,差异均有显著性。银屑病皮损组织中角质层和真表皮交界处C5b-9阳性数显著高于非皮损组织和正常组织;进行期银屑病患者皮损组织角质层和真表皮交界处C5b-9阳性表达数差异无显著性。结论 银屑病的发病以及皮损的严重程度与补体的活化可能有关。     

银屑病患者自身抗体、循环免疫复合物和补体测定

本文报道91例寻常型银屑病患者自身抗体、循环免疫复合物和补体测定结果,证实抗核抗体、甲状腺球蛋白抗体均阴性.RF阳性率为9.09%.循环免疫复合物阳性率为16.66%, CH₅₀正常,C₃含量减少.对自身抗体和免疫复合物在发病机理中的作用作了初步探讨,认为本病患者体内存在自身免疫反应,深入研究银屑病的免疫发病机理,无论在理论上还是临床上都具有十分重要的意义.     

银屑病的免疫学检查和厌氧棒状杆菌菌苗的治疗

作者等对80例不同类型的银屑病病人做了血清免疫学测定,测定结果与对照组比较,经统计学处理显示,E-玫瑰花试验降低,IgG, IgM增高,IgA、总补体活性无明显改变,补体C₃值较正常对照组低.此80例病人应用厌氧棒状杆菌菌苗治疗.有效率达到85多.但其副作用较明显.     

血清可溶性黏附分子与系统性红斑狼疮患者活动性的关系

目的 探讨血清黏附分子中可溶性血管细胞黏附分子-1(sVCAM-1)和可溶性细胞间黏附分子-1(sICAM-1)水平与系统性红斑狼疮(SLE)活动性的关系。方法 sVCAM-1和sICAM-1检测采用酶联免疫吸附试验(ELISA)。结果 ①SLE患者血清sVCAM-1平均水平为2342.45ng/mL,显著高于正常对照组1239.68ng/mL(P<0.001);SLE患者血清sICAM-1平均水平为802.34ng/mL,显著高于正常对照组626.15ng/mL(P<0.001)。②SLE患者血清sVCAM-1水平和sICAM-1水平活动期均高于非活动期(P均<0.001),肾损组均高于非肾损组(P均<0.001)。③SLE组血清sVCAM-1和sICAM-1水平与SLE疾病活动指数(SLEDAI)、抗dsDNA抗体阳性呈正相关,与血清补体C3水平呈负相关。④糖皮质激素治疗后患者sVCAM-1水平和sICAM-1水平均低于治疗前(P均<0.001)。结论 sVCAM-1、sICAM-1可能参与SLE的发病,与SLE活动性相关。     

系统性红斑狼疮与B淋巴细胞异常

以往人们对系统性红斑狼疮(SLE)免疫异常的研究主要集中在抑制性T细胞(T_s)及其免疫调节异常.近年来随着研究的不断深入,人们逐渐对产生自身抗体的效应细胞——B淋巴细胞在SLE免疫异常中的作用有了新的认识.本文拟就(1)SLE B细胞系干细胞水平异常;(2)SLE B细胞的异常激活、增殖和分化;(3)SLE B细胞增殖分化因子的产生异常和SLE中B细胞对B细胞增殖分化因子的异常反应;(4)SLE B细胞多克隆活化的自身刺激作用机制;(5)独特型与SLE发病及多克隆B细胞活化的可能联系等五个方面的有关资料作一综述.     

SLE患者的红细胞免疫粘附功能变化初探

本文应用郭氏法对38例SLE患者进行红细胞免疫粘附功能变化的研究,其中26例活动期患者的红细胞Cab受体花环率明显低于献血员正常值(t=4.71, P<0.001),红细胞免疫复合物花环率明显高于献血员正常值(t=12.21,P<0.001);12例缓解病人与献血员红细胞Cab受体花环率无明显差别(t=0.28, P>0.05),而与其红细胞免疫复合物花环率尚有明显差别(t=3.34, P<0.01).对14例SLE患者用强的松治疗前后测定红细胞免疫粘附功能变化,并对其临床意义进行了初步探讨.     

Complement Components in Recurrent Genital Herpes Simplex

ABSTRACT: Complement components 1,2,3,4 and total complement hemolytical activity CH₅₀ were studied in 39 patients with serve recurrent genital herpes simplex. The results show statistically significant and selective diminution of C₄ (P>0.001) The other components were normal. C₄ has a specific action against herpesvirus by itself or associated to some other elements over C₄ failure, local or systemic disturbances alter the homeostasis of specific and nonspecific immune system, provking a disbalance host-virus which would explain the recurrence.     

SLE患者外周血白细胞B7-H1分子的表达

目的 探讨B7-H1分子在系统性红斑狼疮(SLE)患者外周血白细胞的表达.方法 心用荧光抗体染色技术,经流式细胞仪检测30例SLE患者和20例正常人对照者外周血白细胞表面B7-H1的自然表达水平,同时收集SLE患者的实验室检查指标,并用SLE疾病活动指数(SLEDAI)来判定患者疾病活动程度.结果 活动期SLE组单核细胞、淋巴细胞和粒细胞上B7-H1表达水平均明显高于正常人对照组(P<0.01);SLE患者单核细胞和淋巴细胞B7-H1表达水平与SLEDAI呈正相关(r₁=0.42,P₁<0.05;r₂=0.52,P₂<0.05);抗dsDNA抗体阳性患者组淋巴细胞表面B7-H1分子数较抗dsDNA抗体阴性组明显增高(t=3.20,P<0.01).治疗后SLE患者外周血单核细胞、淋巴细胞和粒细胞B7-H1表达均明显下降,t值分别为10.00、6.75和4.33,P<0.01.结论 活动期SLE患者外周血白细胞B7-H1表达增加,且与SLEDAI成正相关,B7-H1可能与SLE的发病机制有关.     

Abnormalities in the alternative pathway of complement in psoriasis

Some of the immunologic aspects of psoriasis suggest that complement may be involved in the pathophysiology of that disease. The purpose of this work was to examine the complement system in more detail in 20 patients with psoriasis. Classical pathway determinations, RCH50 and factor B levels were normal or elevated. Abnormally low properdin levels were seen in 12/20 patients. No patient had a serum properdin value greater than 1 standard deviation above the control mean. Mean C3–C9 consumption after zymosan or cobra venom incubation was also significantly less than normal (P < 0·001). Thus, abnormalities of the complement system are present in psoriasis and seem limited to the alternative pathway.     

Comprehensive Evaluation of Complement Components in the Course of Type I (Lepra) and Type II (ENL) Reactions

Complement components C1q and C4 of classic pathway; C3d, a breakdown product of C3, and factor B of alternate pathway: and C3, a component both of classic and alternate pathways, were studied in 35 patients, comprising 18 type I (Lepra) and 17 type II (ENL) reactions. There was a significant decrease in C3 and factor B with a concomitant rise of C3d during ENL. These changes indicate their preeminent role in immunogenesis of type II (ENL) reaction. The changes in the classic pathway components, on the other hand, were insignificant, apparently suggesting its limited involvement in ENL. Furthermore, reversion of factor B and C3d after subsidence of reaction is intriguing and may indicate that they are not substantially affected even with contemporary treatment. Complement components, of both classic and alternate pathways, showed no significant alterations either during type I (Lepra) reaction or after its amelioration.     

Complement and immunoglobulin deposits in the skin of patients with atopic dermatitis

The immunofluorescent patterns of uninvolved and involved skin biopsies from eight patients with atopic dermatitis were studied, using direct immunofluorescence techniques to identify deposits of the immunoglobulins G, A and M as well as the complement factors C1q, C3, C4, C5, factor B and properdin. Immunoglobulin deposits (mainly IgG) were found in five patients, complement deposits in three patients in the basement membrane zone. In three patients the immunofluorescence was positive for C3, in two patients for C1q, C4 and C5. Regarding the factors of the alternative pathway of the complement system, two patients showed deposits of properdin, one of factor B. The changes were not confined to the eczematous lesions, but were found in uninvolved skin too. The most prominent changes were observed in patients with severe disease.