花斑癣患者表皮郎格罕细胞的观察

作者采用ATP酶染色方法对10例花斑癣患者皮损表皮内郎格罕细胞(LC)进行了观察,并对4例患者与皮损相对应部位的正常皮肤进行了同样检查.与此同时,取4名正常人胸腹部正常皮肤作对照检查.结果表明:花斑癣皮损表皮内LC密度明显减少,与正常对照组相比,P<0.001,有灶性集聚现象,许多LC的树枝突缩短、减少或消失.4例花斑癣患者与皮损相对应部位的正常皮肤表皮内LC的变化与皮损表皮内LC的变化相类似.作者认为,LC在花斑癣的免疫病理中可能起着重要作用.     

大鼠郎格罕氏细胞Ia抗原及ATP酶的研究

本文报告于大鼠LC表面也具有Ia抗原及ATP酶活性;用Ia抗原间接免疫荧光染色可清晰地显示成年大鼠表皮内的LC:ATP酶染色可用于新生大鼠表皮内LC的检查.本文详细叙述了用I成原间接免疫荧光染色、Ia抗厚间接免疫过氧化酶染色及ATP酶染色显示大鼠表皮内LC的步骤与注意事项.     

类固醇及细胞毒药物治疗后人郎格罕细胞的减少和再现

近年联合使用Ia抗原、ATP酶、Fc和C_3受体鉴别LC的工作,表明糖皮质激素能使动物或人表皮LC数目减少。豚鼠皮肤实验发现LC早在类固醇初次局部处理的2天后就开始下降,14天达到稳定状态,继续用药,LC不再减少。本文作者在人体以LC的Ia、T_6和S-100抗原为指标,研究局部S-100使用0.05％17-戊-倍他米松霜(BV)和全身使用具有骨髓抑制作用的细胞毒药物后对临床上正常皮肤LC数目和着色特性的影响。结果表明,使用BV的24小时内,用抗Ia染色的LC数目即见下降,至第2天,即有显著减少,7天后,LC下降接近极点,约为用药前的56％,若继续用药1周,LC数目不再进一步减少,停药后,3例受试者中的2例Ia着色细胞数目恢复至正常,1例增加。与前者不同,用抗T_6染色,LC数下降较     

郎格罕细胞与皮肤病

郎格罕细胞(LC)是一种树枝状细胞,不仅位于表皮层,也见于真皮及附属器、粘膜、淋巴管、淋巴结、胸腺以及毛细血管内.正常人表皮LC密度为400～1,000个/mm~2,占表皮细胞的3～8%.LC特征性的电镜标记是Birbeck颗粒,组化标记是ATP酶,免疫学标记是Ⅰa抗原和OKT_6抗原.可通过电镜、组化和免疫荧光或免疫酶法鉴定LC.     

疥疮表皮郎格罕氏细胞的光镜和电镜观察

我们用ATP酶染色和透射电子显微镜观察6例疥疮患者的丘疹表皮内郎格罕细胞(LCs).其中4例LCs密度明显降低,有灶性集聚现象,许多LCs的树枝突缩短、减少或消失.3例用透射电子显微镜进行了观察,2例Birbeck颗粒形态异常,1例Birbeck颗粒增多.我们认为LCs在疥疮的免疫病理中起着重要作用.     

湿疹及皮炎

941325 性激素对豚鼠表皮朗格罕细胞及变应性接触性皮炎的影响/金慧玲//中华皮肤科杂志。-1994,27 (2).-89～90 观察了丙酸睾丸酮对豚鼠表皮LC密度及变应性     

UVA照射人表皮后郎格罕细胞数目及形态的变化

作者曾报告在正常表皮中,3种不同的细胞表面标记物(OKT6,HLA-DR,ATP 酶)标记的郎格罕细胞(LC)并无明显差异。然而在有病的表皮中,HLA-DR,ATP 酶对 LC 的标记是不可靠的。由于 OKT6并不与骨髓细胞及正常的外周单一核细胞发生反应,因而对有病变的皮肤中,LC 可能是一种特殊的、有价值的标记物。UVA 对LC 的作用尚未作相应的研究。本文作者以2.5倍最小红斑量 UVA(平均 MED_A=50J/cm~2)照射人     

在人和鼠表皮片中计算朗格罕氏细胞的一个方法

朗格罕氏细胞是位于表皮内的树枝状细胞,其功能尚有争论.以往认为它是衰老退化的色素细胞,近年来的研究表明它是表皮免疫系统的一个主要成分,似乎有免疫巨噬细胞功能,参与皮肤免疫反应.鉴别朗格罕氏细胞最早用氯化金染色法,此后一系列方法中最常用ATP酶技术染色,但此法有重大的缺点:(1)对朗格罕氏细胞无特异性,可将朗格罕氏细胞和黑素细胞均染成树枝状深褐黑色.(2)染色不均匀,不持久.故此法不能用来确定朗格罕氏细胞数.为此作者设计一种特异而且敏感的技术.免疫荧光技术仅能染表皮中带Ia抗原的细胞即朗格罕氏细胞和未定类细胞.作者用改良间接免疫荧光染色     

表皮细胞衍生的细胞因子及其免疫学作用

表皮不仅是外界环境和生物体内环境之间的机械性屏障,而且具有活跃的免疫学功能。例如,表皮郎格罕细胞(LC)可向T 淋巴细胞呈递抗原,诱发抗原特异性免疫反应,在接触性皮炎的发病中起着重要作用;I-J阳性表皮树突状细胞则优先诱导抗原特异性     

皮肤红斑狼疮中的表皮郎格罕细胞

为研究表皮郎格罕细胞(LC)在皮肤红斑狼疮(LE)中的作用,作者通过Dermovac装置作真空诱疱,取其疱顶表皮,用组织化学染色法和间接免疫荧光法分别检测LC的三种表面标志:三磷酸腺苷酶(ATP酶)活性、HLA-DR抗原和OKT-6抗原,以及用混合表皮细胞—淋巴细胞培养测定氚胸腺嘧啶核苷的掺入,检测从皮肤LE病人分离的表皮细胞刺激同种异体淋巴细胞的增殖能力,对皮肤LE病人皮损和非皮损表皮中的LC进行了研究.受检的皮肤LE病人共7例,其中5例为活动性亚急     

Book Reviews

Books reviewed in this article:
Clinical Dermatology. Diagnosis and Therapy of Common Disease. By P. V asarinsh .
Manual of Practical Dermatology. By J ohn H.S. P ettit .
Colour Atlas and Synopsis of Clinical Dermatology. By T.B. F itzpatrick , M.K. P olano and D. S uuhmond .
Virus Diseases and the Skin. By T.W.E. R objnson and R.B. heath .
Vitiligo and other Hypomelanoses of the Hair and Skin. By J.P. O rtonne , D.B. mosher and T.B. F itzpatrick .
Update: Dermatology in General Medicine. By T.B. F itzpatrick , A.Z. E isen , K. W olff , I.M. F reedberg and F.K. A nsten .     

BOOK REVIEWS

Book reviewed in this article:
COSMETICS SCIENCE AND TECHNOLOGY. Edited by M. S. Balsam and Edward Sagarin.
FUNDAMENTALS OF MEDICAL BACTERIOLOGY AND MYCOLOGY FOR STUDENTS OF MEDICINE AND RELATED SCIENCES. Quentin N. Myrvik, Nancy N. Pearsall, and Russell S. Weiser.
VASCULAR DISORDERS OF THE EXTREMITIES. David I. Abramson, M.D., F.A.C.P.
PRINCIPLES OF MODERN IMMUNOLOGY. Byling H. Park and Robert A. Good.
THE MISSOURI RIVER: THE RIVER RAT'S GUIDE TO MISSOURI RIVER HISTORY AND FOLKLORE. Richard L. Sutton, Jr. and Kenneth R. Canfield.
MICROBIOLOGY OF HUMAN SKIN. W. C. Noble and Dorothy A. Somerville.
LEPROSY. DIAGNOSIS AND MANAGEMENT. Harry L. Arnold, Jr. and Paul Fasal.
SYPHILIS AND OTHER VENEREAL DISEASES. William J. Brown, James F. Donohue, Norman W. Axnick, Joseph H. Blount, Oscar C. Jones, Neal H. Ewen.
MYCOSES IN MAN. N. D. Shekalov, and M. V. Milich.
NAIL DISEASES IN INTERNAL MEDICINE. Pietro de Nicola, Mario Morsiani and Giorgio Zavagli.     

BOOK REVIEWS

Book reviews in this article:
Histopathology of the Skin. W. F. L ever .
Atlas of the Histopathology of the Skin . G. H. P ercival , G. L. M ontgomery and T. C. D odds .
Year Book of Dermatology (1961–62). Ed. R. L. B aer and V. H. W itten .
Dermatology for Students . Ed. R. O. N oojin .
Systemic Lupus Erythematosus . D. L. L akson .
The Post-throvibotic Syndrome . R. J. P opkin .
Therapie der Haut- und Geschlechtskrankheiten . Ed. J. K immig .     

BOOKS RECEIVED

Migraine and other Common Neuroses. F. G. C rookshank .
Medical Electricity and Radiology. J. R. R iddell , F. R. F. P. S.
Diseases of the Skin. R ichard L. S utton , M.D., LL.D., F. R. S. Edin.
Geschlechtskrankheiten Bei Kindern. A. B uschke and M. G umpert .
Hautkrankheiten. Dr. G eorg A lexander R ost .     

Book Reviews

Books reviewed in this article:
Dermatology for the Physician—an Illustrated Guide . E. L. R hodes .
The Treatment of Mycosis with Imidazole Derivates . By W.P.E. R aab . Foreword by A.M. Kligman.
Manual of Dermatology. An Introduction to Diagnosis and Treatment . By J.P. C allen , M.A. S trawiski and J. H. V oorhees .
Photochemotherapy–Information for Doctors and Patients . By G. W eber .
Diagnosis of Skin Disease . Compiled by G erald S. L azarus and L owell A.G oldsmith .
Handbuch der Haut- und Geschlechls-krankheiten. Nicht Entzundliche Dermatosen III B. , J. J adassohn . Edited by H.A. G ottron and G.W. K orting .
Malignant Lymphomas other then Hodgkin's Disease . K. L ennert .
Histological Diagnosis of Inflammatory Skin Disease . By A. B ernard A ckerman .     

Book Reviews

Book reviewed in this article:
DERMATOLOGY. Cerd Klaus Steigleder and Howard Maibach.
DISEASES OF THE HAIR AND SCALP. Arthur Rook and Rodney Dawber.
THE CUTANEOUS ARTERIES OF THE HUMAN BODY. Carl Manchot.
AIDS: THE MYSTERY A N D THE SOLUTION. Alan Cantwell, Jr., M.D.
WHERE NO SKIN SPECIALIST IS AVAILABLE. P. N. Behl.
TRIES, Volumes I and II. Monica Cheesbrough. 520 and 488 pages.
POCKET ATLAS OF DERMATOLOGY. Cerd Klaus Steigleder and Howard I. Maibach.
DERMATOLOGY AND VENEROLOGY. O. Braun-Falco, C. Plewig, and H.H. Wolff.
MANUAL DE DERMATOLOCIA: NINTH EDITION. Juan C. Gatti, M.D., and Jose E. Cardama, M.D.
LEPROSY IN MALAYSIA. A. Joshua-Raghavar.
ATLAS OF SKIN DISEASES OF THE HORSE. Leopoldo F. Montes and J. Thomas Vaughan.
PHOTOIMMUNOLOCY. John A. Parrish, Margaret L. Kripke, and Warwick L. Morison.     

BOOK REVIEWS

Book reviewed in this article:
ADVANCES IN MODERN TOXICOLOGY, VOL. 4. DERMATOTOXICOLOGY AND PHARMACOLOGY. F. N. Marzulli and H. I. Maibach.
FUNDAMENTALS OF CLINICAL IMMUNOLOGY. J. Wesley Alexander and Robert A. Good.
DERMATOLOGY SPECIALTY BOARD REVIEW. 3rd ed. Frederick A. Pereira, M.D. and Victor J. Selmanowitz, M.D.
ATLAS OF AQUATIC DERMATOLOGY. Alexander A. Fisher, M.D.
HISTOLOGIC DIAGNOSIS OF INFLAMMATORY SKIN DISEASES. A. Bernard Ackerman, M.D.
CURRENT MEDICAL DIAGNOSIS AND TREATMENT 1979. Marcus A. Krupp and Milton J. Chatton.     

Book Reviews

Books reviewed in this article:
A Colour Atlas of Diseases of the Vulva. By C.M. R idley , J. D. O riel , and A.J. R obinson , Published by Chapman & Hall Medical, London, 1990. Price: £60.00.
A Colour Atlas of Allergic Skin Disorders. By R. C cerio and W.F. J ackson , Published by Wolf Publishers, London.
Healthy Skin—the Facts. By R. M ac A ie , Published by Oxford University Press, Oxford, 1992. ISBN 0-1926-2244-7.
The Skin Microflora and Microbial Disease. Edited by W.C. N oble , Published by Cambridge University Press, 1993. Pp. 389. Price: £60.
Year Book of Dermatology 1992. Edited, by A.J. S ober and T.B. F itzpatrick . Published by Mosby Year Book, Inc., 1992 Pp. 535. ISBN 0-8151-7914-6.
Cutaneous Drug Reactions. Edited by K. Z ürcher and A. K rebs . Pubished by Karger, Basel, 1992. Pp. 570 Price £215.70.
Human Body Odor. Etiology, treatment and related factors. By M. I naba and Y. I naba . Published by Springer-Verlag, Tokyo, 1992. Pp. 285. DM 248.
Epidermolysis Bullosa: Basic and Clinical Aspects. Edited by A.N. L in and D. M artin C arter . Published by Springer-Verlag, 1992.     

Book Reviews

Books reviewed in this article:
Manual of Therapy for Skin Diseases. BY T.G.B erger , P.M. E lias and B.U.W introub .
The Essentials of Dermatology , 3rd edn. J.L.B urton .
Handbook of Paediatric Dermatology , 2nd edn. By J.H arper .
Handbook of Dermatology. By D.J.B arker .
Skin Immune System (S.I.S.). Edited by J.D.B oss .
Methods for Skin Absorption. Edited by B.W.K emppainen and W.G. R eifenrath .     

BOOKS RECEIVED

Artificial Sunlight and its Therapeutic Uses. By F. H. H umphris , M.D. Brux., F.R.C.P. Edin.
Ultra-Violet Rays in the Treatment and Cure of Disease. By P. H all , M.R.C.S.
Handbuch der Salvarsantherapie. By W. K olle and K. Z ieler .
Die Beziehungen der genitalorgaue zu Hautveränderungen. By Dr. K urt W iener .
Der Ausfall des Kopfhaars und seine Behandlung. By Dr. F elix P inkus .
Beziehungen der ekzematösen Erkranhungen zu inneren Leiden. By Dr. S. E hrmann .