系统性硬皮病中抗核仁抗原自身抗体与临床的关系

抗核仁抗体是系统性硬皮病(SSc)和特征性标记.已确定PM-Scl,U3RNP,RNA多聚酶Ⅰ和RNaseP是4种主要的核仁抗原,可用免疫沉淀或双向免疫扩散(DID)测定抗这些抗原的抗体,亦可用间接免疫荧光(IIF)在HEp-2细胞上确认它们的特征性类型.迄今关于核仁自身抗体的类型与SSc临床表现间关系的报告甚少.为此,作者以HEp-2细胞为底物,用IIF检测了296例SSc、40例MCTD和214例SLE的血清.结果发现64例患者的血清有核仁型免疫荧光染色,其中49例为SSc,15例为SLE,无1例为MCTD.作者对这64例患者     

多种底物在免疫金银染色检测抗核抗体试验中的联合应用

将多种底物联合应用于免疫金银染色检测ANA试验,与各底物单独应用比较,可明显改善检测的敏感性与核型显示。其中,两种鼠脏器印片联合应用的结果可与Hep-2细胞的结果相当,三种及四种鼠脏器印片联合应用的结果可与Hep-2细胞和一种鼠脏器印片联合应用的结果相当,且均不使正常人ANA阳性率明显升高。     

抗着丝粒抗体和抗局部同分异构酶Ⅰ抗体与临床的联系

为进一步研究抗着丝粒抗体(ACA)和抗局部同分异构酶I(topoisomerase I,简称topo I)抗体与临床的联系,作者对355例病人的血清进行了研究。其中89例为近心端硬皮病,54例CREST综合征,154例原发或继发性雷诺氏病。另58例患SLE、干燥综合征或RA,但无雷诺氏现象,任何CREST 表现、皮肤变厚或发紧,这些病人作为结缔组织病(CTD)的对照组。34名健康人为另一对照组。以HEp-2细胞作底物,用间接免疫荧光法测ACA;以免疫扩散法测抗topo I(Scl-70)抗体;以提纯的HeLa 细胞染色体作为底物,用免疫印迹法检测ACA 及抗topo I。结果:①CTD 对照组及健康人对照组ACA 阴件,仅1名健康人用免疫印迹法检测抗topo I 抗体,结果阳性,CTD 对照组未检出此抗体。用两     

糜烂性扁平苔藓患者可有抗表皮细胞核抗原的抗体:有关抗原性质的研究

本文报道2例糜烂性扁平苔藓(LP)伴潜在性HBV感染具有循环抗表皮细胞核抗体的患者.例1.女性,64岁,口腔粘膜糜烂性LP及四肢LP皮损伴慢性肝炎早期硬化,HBsAg阴性,但抗HBs及抗HBc抗体阳性.例2.女性,53岁,口腔粘膜糜烂性LP及左颞部基底细胞癌(经病理证实),HBsAg阴性,但抗HBs及抗EBVIgG抗体阳性.2例患者的损害病理检查均示LP改变.直接免疫荧光(DIF)检查示表皮细胞核内有斑点状IgG沉积.间接免疫荧光(IIF)检查示:以大鼠肝为底物抗核抗体(ANA)阴性,以猴食道为底物ANA阳性.     

614例系统性红斑狼疮自身抗体检测结果分析

目的探讨抗核抗体(ANA)、抗ENA抗体和抗ds-DNA抗体对系统性红斑狼疮(SLE)的临床实用价值和特点.方法ANA和抗ds-DNA抗体采用间接免疫荧光法检测.ANA底物为小鼠肝肾印片;ds-DNA抗体的实验基质为血鞭毛虫属的绿蝇短膜虫;抗ENA抗体检测采用酶免疫斑点(条带)实验.结果ANA阳性率为98.05%,ds-DNA抗体阳性率为60.10%,抗Sm抗体阳性率为46.58%,均高于其他自身抗体的阳性率,x2检验有统计学意义(x2=17.00,P＜0.05).结论ANA作为SLE重要的一类抗体,ds-DNA抗体和抗Sm抗体是SLE的重要标志性抗体,三种抗体联合测定可提高SLE的检出率,对SLE病情的诊断、分型、治疗、追踪和预后判定均有重要价值.     

参芪片对尖锐湿疣患者外周血T细胞亚群的影响

尖锐湿疣(CA)是由人乳头瘤病毒(HPV)引起的性传播疾病,难以根治.1参芪片系由人参、黄芪、鹿角、热地黄、枸杞子等中药组成,人参皂甙等有效成分具有明显提高小鼠非特异性免疫功能和特异性免疫功能的作用.2黄芪不仅能增加小鼠抗体生成器官脾脏的重量、脾细胞数及抗体反应,还能完全对抗甲泼尼松龙所致的脾脏、胸腺及肠淋巴结等免疫器官的显著萎缩及外周白细胞的减少,促进抗体生成,诱导小鼠脾细胞产生IFN-γ, 提高溶血素抗体的水平.     

Ro、La抗原的部分生物学特性

本文对Ro,La抗原的一些生物学特性进行了研究.在哺乳类动物(犬、小牛、猪、兔及豚鼠)和人组织中的Ro,La抗原较易测得,而大鼠和小鼠中的Ro,La抗原则不易测得,说明Ro,La抗原在种系间存在量的差异.Ro,La抗原广泛存在于人体各组织器官中,是细胞的基本成分,但在不同组织及器官中的分布不均匀,且这种分布与临床上SLE病人的器官受累频率无一致性.以人胎肝为底物进行间接免疫荧光检查时,Ro抗原主要表现为胞浆内荧光,而以大鼠、小鼠肝为底物时则为阴性,La抗原在人胎肝印片中表现为较强的胞浆和胞核(均质型)染色,而在大鼠和小鼠肝中则表现为较弱的粗斑点线型核荧光,表明在不同的哺乳动物中,抗原可能还存在部位的差别.     

抗Ro/SSA抗体——与颗粒状(大斑点样线 状)免疫荧光核染色型的联系

作者用标准的双向免疫扩散试验,以人脾盐水提取物为抗原(Ro/SSA抗原)检测患者血清中的抗Ro/SSA抗体,并用Burnham推荐的间接免疫荧光法,以人脾EP印片作底物,检测抗核抗体(ANA),研究血清抗Ro/SSA抗体与颗粒状,即“大斑点样线状”核染色型间的联系。系统性红斑狼疮(SLE)、亚急性皮肤红斑狼疮(SCLE)患者和白细胞碎裂性血管炎病人的血清被检。结果:21例SCLE和1例干     

皮肤科学基础

20 0 4 0 6 18　基因工程抗角蛋白抗体在正常皮肤和几种表皮增生性皮肤病皮损中的反应定位/卢宁(四军大西京医院皮肤科)…∥中国皮肤性病学杂志.- 2 0 0 3,17( 4) .- 2 2 1利用从噬菌体抗体库中筛选到的特异表达抗角蛋白Fab片段的质粒转化大肠杆菌,IPTG诱导表达出Fab抗体,纯化鉴定后用此抗体对正常皮肤及银屑病、鳞癌、基底细胞癌和脂溢性角化病皮损进行免疫组化染色。正常皮肤表皮呈阴性染色,毛囊呈阳性染色,银屑病、鳞癌、基底细胞癌和脂溢性角化病皮损均呈现明显的阳性着色,其中银屑病皮损基底细胞层为强阳性。所有细胞染色部位均位于…     

天然免疫性自身免疫病:炎症性皮肤病的新学说?

按照既往认识,自身免疫病属于获得性免疫的范畴,在患者外周血或器官、组织内必须存在源于自身的特异性的抗原和与之识别的特异性抗体或以细胞毒性T细胞为主的淋巴细胞.根据抗原-抗体和受累器官/细胞是否具有特异性,自身免疫病又分为器官特异性和器官非特异性.     

Anti-Ro/SSA antibodies. Association with a particulate (large speckledlike thread) immunofluorescent nuclear staining pattern

Twenty-one patients with clinical and histopathologic evidence of subacute cutaneous lupus erythematosus and one patient with Sj?gren's syndrome and vasculitis had anti-Ro/SSA (Sj?gren's syndrome A) antibodies as demonstrated by double immunodiffusion assay using a saline extract of human spleen as the source of antigen. These serum samples were negative when tested for other nuclear antigens, including native DNA, Sm, and RNP. When tested for antinuclear antibodies (ANAs) by immunofluorescence, only ten of 22 serum samples were ANA positive on mouse liver substrate, while 18 of 22 had a positive ANA when HEp-2 tumor cells were utilized. With imprints of human spleen as test substrate, all 22 serum samples yielded a positive ANA result and a particulate (large speckledlike thread) staining pattern. Absorption of two of these serum samples with human spleen extract containing Ro/SSA antigen inhibited both the particulate staining pattern using human spleen imprints and the anti-Ro/SSA precipitin line by double immunodiffusion. These studies suggest that anti-Ro/SSA antibodies and antibodies producing the particulate nuclear staining pattern on human spleen imprints are either one and the same or closely paralleling antibody systems.     

The fibrillarin (Scl-34) autoantibody in systemic scleroderma

Reexamination of ANA positive sera with nucleolare fluorescence staining on HEp-2 cells and hamster liver imprints revealed 3 sera with an uniform characteristic pattern. It is characterized by: 1. Strong clumpy fluorescence of nucleoli in the interphase. 2. Missing nucleoplasma staining (pure nucleolar). 3. Characteristic granular staining of the cytoplasma from the condensed chromosoma in mitosis. 4. Additive fluorescence of a nucleolus-like body. 5. Extremely high antibody titers. 6. Negative immunodiffusion. This nucleolar staining pattern was described by Bernstein et al. as a clumpy nucleolar pattern. It is produced through an antibody to the dense fibrillar component of the nucleolus (fibrillarin). This antigen is a protein of the U3 RNP with a molecular weight 34 kDa. Clinically is the fibrillarin antibody highly specific for systemic scleroderma especially with diffuse skin involvement. We found it in 1% of scleroderma patients. Its pathogenetic importance is not known.     

Substrate specificity of antinuclear antibodies in scleroderma.

Studies of antinuclear antibodies (ANA) were carried out in 39 cases of systemic scleroderma and for comparison in 19 cases of systemic lupus erythematosus (SLE) and 4 of mixed connective tissue disease (MCTD) using indirect immunofluorescence (IF) methods under standard conditions. The results on three different substrates--monkey esophagus, guineapig lip and rat liver--are reported. In 48.7% of scleroderma cases ANA showed a substrate specificity. The highest percentage of positive results in scleroderma was obtained on monkey esophagus (97.4%) and the lowest on rat liver (61.5%). In SLE and MCTD, in contrast, only about 13% of the sera displayed such specificity. If only sera with substrate specificity are considered, the positive results on monkey esophagus and rat liver are 94.7% and 21.1%, respectively. Titers of sera reacting positively on 2 or 3 substrates were mostly in agreement, although some sera both in systemic scleroderma and SLE showed higher titers on monkey esophagus. The IF pattern was usually the same regardless of the substrate, Tests for ANA in scleroderma should be performed on at least 2 substrates simultaneously.     

Nuclear antibodies as serologic markers in progressive systemic scleroderma

In all, 36 patients with progressive systemic sclerosis (29 women, 7 men) were studied clinically and immunologically; 15 patients had acrosclerosis (type I) and 21, sclerosis extending beyond the wrist (type II). The sera of all patients were evaluated for ANA (HEp-2-cells), Scl-70, centromere and other ENA antibodies. The centromere antigen was characterized by immunoblotting. All patients had high-titer ANA antibodies (100%); 36% of patients had the Scl-70 antibody (a marker antibody for PSS); and in 22% of our patients a centromere antibody was detected. In all cases the anti-centromere sera reacted with a 19.5-kd polypeptide and in 2 cases they reacted with 23- and 25.5-kd proteins in addition. In patients with centromere antibodies there was increased organ involvement (heart, lung, kidney) compared with patients who had anti-Scl-70 or other nuclear antibodies.     

Scl 70 antibody—a specific marker of systemic sclerosis

Scl 70 antibodies were tested for in 107 patients with systemic sclerosis: 68 with acrosclerosis and 39 with diffuse scleroderma. Anticentromere antibodies (ACA) and other antinuclear antibodies (ANA) were tested for by indirect immunofluorescence on HEp-2 cells. Positive results for Scl 70 antibodies were obtained in 77% of cases of diffuse scleroderma and 44% of acrosclerosis. ACA and Scl 70 antibodies were found to be mutually exclusive. If acrosclerosis cases positive for anticentromere antibodies are excluded, the percentage of acrosclerosis cases positive for Scl 70 was 63%. ACA were found to be a marker of a benign, abortive subset of acrosclerosis with almost no cutaneous involvement (CREST), whereas Scl 70 did not discriminate between acrosclerosis and diffuse scleroderma. On HEp-2 cells Scl 70 positive sera gave a characteristic, fine speckled, almost homogeneous nuclear staining pattern.     

用Hep-2细胞作抗原检测抗核抗体——FANA试验和FLISA

本文提出在酶标板小孔内培养Hep-2细胞的方法制作抗原,用于免疫酶联吸附检测法(ELISA)测定血清抗核抗体(ANA),并用同一抗原作FANA试验对两种方法检测ANA的敏感性进行比较,共检测血清22份(SLE8份、其他疾病9份,正常对照5份)。检测结果表明二种试验方法的特异性一致,ELISA检测血清ANA的敏感性比FANA试验显著提高,该方法特异性强、敏感性高、简便、更适用于微量法。     

Immunologic sequelae of thermal injury autoantibodies in acute adult burn patients

Summary Indirect immunofluorescent (IF) studies have demonstrated the presence of antinuclear antibodies (ANA) and antibodies reactive with the intercellular area (IC), basement membrane zone, and basal cells of autologous and allogeneic unburned skin in the sera of acute adult burn patients. Further demonstration in burn patients of the occurrence of peripheral ANA masquerading as IC antibodies is presented. In keeping with previous IF identifications of autoantibodies in burn patients, there was no significant relationship between the frequency and titer of these antibodies according to the extent of total body surface burn area and third degree burn area. The possible role of epithelial antibodies in the maintenance of host resistance, as previously suggested, is considered in brief.Presented in part at the workshop on the Immune Consequences of Thermal Injury, Lake Arrowhead, California, 2–5 December, 1979     

Immunofluorescence studies in progressive systemic sclerosis (scleroderma) and mixed connective tissue disease

Immunofluorescence (IF) investigations of the skin were performed in thirty patients with progressive systemic sclerosis (scleroderma) and eight patients with mixed connective tissue disease (MCTD). The results show that speckled epidermal nuclear immunoglobulin deposition occurs not only in MCTD but also in true scleroderma. Granular IgM deposition at the dermo-epidermal junction of light-exposed skin was detected in both groups of patients, but six of eight MCTD patients also showed a granular IgM band in non-exposed skin. Antinuclear antibodies (ANA) were demonstrated in the sera of 96% and 100% of patients with scleroderma and MCTD respectively. The pattern of nuclear IF staining in scleroderma included dense fine speckles, large coarse speckles, threads, nucleolar and centromere staining. In MCTD, by contrast, the ANA staining pattern consisted of threads. The significance of ANA titres and immunological specificities for the in vivo reaction of serum ANA with epidermal nuclear antigens is discussed.     

Antinuclear antibodies in patients with lichen planus

Abstract Lichen planus (LP) is a mucocutaneous syndrome of yet uncertain pathogenesis, and it has usually been considered to be a dermatosis without antinuclear antibodies (ANA) nor other specific auto-antibodies. Over 10 years ago a series of indirect immunofluorescence researches with patients' lesional skin and serum disclosed the presence of lichen planus specific antigens (LPSA). After this, a number of substrates have been submitted for evaluation. In this study we have carried out indirect immunofluorescent test in relation with different substrates, with the aim of verifying whether the negative results previously obtained were due to poor sensitivity of the substrates employed. Subsequently we have compared the results obtained in the erosive forms of LP with those obtained in the non-erosive forms. We have concluded that rat oesophagus is a better substrate for the detection of ANA in patients with LP, as it has proved to have a positivity rate of 40.42%. Monkey oesophagus has provided a rate of 27.6%, and HEP-2 cells and rat liver have proved to be unsuitable. In addition, we have observed that the frequency of ANA is higher in the erosive forms of LP (P= 0.0389). In this article we demonstrate that the presence of ANA in patients with LP depends on the substrate employed, the most suitable substrate being rat oesophagus. Also, we demonstrate that ANA is more frequently observed in patients with erosive LP.