正常人血清抗角蛋白抗体免疫组织化学的研究

本文以正常人皮肤冰冻切片为抗原组织,用间接免疫荧光技术,发现正常成人血清中广泛存在一种抗角蛋白的自身抗体。该抗体的阳性反应主要发生于角层与粒层之间。分别用IgG、IgA和IgM荧光抗体检测正常成人血清105份、28份、28份,其发生率IgG66.67%、IgA100%、IgM96.43%。抗角蛋白抗体无种的特异性,但有器官特异性,其发生率与性别、血型无关,但似乎随年龄递增。     

正常人血清抗角蛋白抗体的免疫组织化学研究

本文以正常人皮肤冰冻切片为抗原组织,用间接免疫荧光技术发现正常成人血清中广泛存在一种抗角蛋白的自身抗体,该抗体的阳性反应主要发生于角层与粒层之间.分别用IgG,A,M检测正常成人血清105份、28份、28份,其发生率IgG66.67%,IgA100%,IgM96.43%.抗角蛋白抗体无种属的特异,但有器官特异性,其发生率与性别、血型无关.     

银屑病患者血清中抗溶血性链球菌抗体的类别及其意义

目的：探讨银屑病患者血清中抗β－溶血性链球菌抗体的类别及其意义。方法：用ELISA法测定各型银屑病患者及正常对照血清中抗β－溶血性链球菌相应的抗体IgG、IgM、IgA。结果：各型银屑病患者血清中存在不同水平的抗β－溶血性链球菌之相应的IgG、IgM、IgA类抗体；与正常对照相比，IgM类抗体各型银屑病均高于正常对照，并有统计学差异（P＜0.05）；银屑病各型之间的比较，点滴状和斑块状银屑病与其余型别不同，IgG类抗体占优势。结论：在各型银屑病中虽然抗β－溶血性链球菌各类别抗体水平不同，但趋势一致，特别是IgM类抗体的检测提示此微生物在银屑病病因的可能一致性，其中以点滴状银屑病为一典型的代表。     

银屑病的免疫病理学:与其他角化不全性病变的比较

银屑病的免疫病理机制已有一些文献报告,在体内IgG和补体C_3与病变角层结合,也见到IgM和IgA存在于损害部位.银屑病人血清已发现有循环IgG和IgM类抗角层细胞间抗原的抗体,也见于正常人.此抗体可以固定补体.在泛发性角层下水疱形成的患者和红皮病患者,此抗体随病情的改变而增减.IgG沉着的方式与损害中角层抗原的分布一致,可代表该抗原抗体在体内结合的位置.有人认为银屑病角层抗原由于表皮受到损伤而暴露或活     

银屑病患者血清抗EB病毒抗体的检测

目的：探讨EB病毒感染与银屑病的关系。方法：采用酶免疫法检测53例银屑病患者血清EB病毒抗壳抗原／IgG、IgM抗体，抗早期抗原－D／IgG抗体。抗核心抗原－1／IgG抗体。结果：银屑病患者血清抗ER病毒早期抗原－D／IgG抗体阳性率明显高于正常对照组（P＜0．001）。结论：银屑病患者体内EB病毒可能处于激活状态。     

各型银屑病患者血清中抗结核分枝杆菌及其提取物抗体水平的研究

目的：探讨各型银屑病患者血清中抗结核杆菌及其提取物的抗体水平和可能的意义。方法：用ELISA法测定各型银屑病患者及正常对照血清中抗结核杆菌的整菌、培养液和胞浆提取物的Igc、IgM和IgA抗体水平。结果：各型银屑病患者血清中检出不同水平的抗此菌三类成份的抗体，常见型（点滴状、斑块状）银屑病的IgG抗体，少见型（红皮病型、脓疱型、关节病型）的IgA抗体水平与正常对照相比差异有统计学意义（P〈0．05），常见型银屑病和少见型银屑病IgM水平皆高于正常对照组，且有统计学差异（P〈0．05）。而且，这些与正常对照有差异的银屑病患者与肺结核病患者比较无统计学差异（P〉0．05）。结论：银屑病患者血清中存在与肺结核病患者差异不明显的抗结核杆菌整菌、培养液和胞浆提取物的IgG、IgM和IgA抗体水平，三类抗体在各型银屑病中的抗体水平可能不同。     

SLE患者血清抗磷脂抗体的分布及临床意义

目的:探讨抗磷脂抗体(APA)在SLE患者血清中的分布及临床意义。方法:应用酶联免疫吸附实验(ELISA)法检测64例正常人和56例SLE患者血清中6种APA。结果:SLE患者IgG型抗心磷脂抗体(aCL)、抗磷脂酰肌醇抗体(aPI)、抗磷脂酸抗体(aPA)、抗磷脂酰丝氨酸抗体(aPS)、抗磷脂酰胆碱抗体(aPC)的A值和阳性率均显著高于正常人;IgM型aCL、aPA、aPE、aPS、aPC的A值和阳性率均显著高于正常人。SLE时IgG型和IgM型APA的总阳性率分别为67.86%和69.65%。结论:正常人血清中存在低滴度的APA,SLE患者血清中APA的阳性率高于正常人。APA的检测对早期诊断SLE可能有一定意义。     

银屑病患者血清中抗β-溶血性链球菌及其相关提取物抗体状态的研究

ELISA法测定各型银屑病患者及正常对照血清中抗β-溶血性链球菌的整菌、培养液和胞浆提取物的IgG、IgM和IgA类抗体。显示各型银屑病患者血清中存在不同水平的抗此菌3类成分的抗体,3类成分的IgM类抗体在各型银屑病均高于正常对照(P<0.05);银屑病各型之间的比较,在3类成分中主要是点滴状和斑块状银屑病的IgG类抗体与其余型别不同,明显升高(P<0.05),但两者之间无差异。研究表明各型银屑病中抗β-溶血性链球菌及其相关提取物的抗体水平可能不同,IgM类抗体的检测提示此微生物与银屑病病因相关的可能一致性,抗β-溶血性链球菌整菌的IgG抗体与银屑病的型别可能有关联。     

抗三种微生物血清抗体与寻常型银屑病相关性的研究

目的：探讨嗜脂性马拉色菌(P．ovale)、β-溶血性链球菌(β-HS)和人型结核分支杆菌(H37Rv，Mtb)与银屑病的相关性。方法：用三种微生物的整菌作为抗原，以间接酶联免疫吸附试验(ELISA)检测71例斑块状(PP)和103例点滴状(PG)银屑病以及103例正常对照者血清中抗P．ovale，β—HS和Mtb的三类抗体(IgA、IgG、IgM)水平。结果：抗三种微生物的三类抗体在PP、PG患者血清中含量均高于正常对照组，三类抗体的含量从高到低为IgG→IgM→IgA；显著性检验表明，抗P．ovale和β—HS的趋势一致；抗Mtb三类抗体中IgG和IgM在PG、PP的趋势一致，也与抗P．ovale和β—HS的一致，但IgA在PP、PG与正常对照组比较均无显著性差异。结论：在寻常型银屑病患者血清中存在抗P．ovale，β-HS和Mtb的IgG、IgM和IgA类抗体。结果和分析表明抗三种微生物的抗体与寻常型银屑病有相关性，但其作用机制以及为什么对三种微生物都有相关性等尚待深入研究。     

寻常型银屑病与单纯疱疹病毒1型相关性的研究

目的 探讨寻常型银屑病与单纯疱疹病毒1型(HSV-1)的可能相关性。方法 应用PCR法检测患者皮损、外周血单一核细胞(PBMCs)和咽拭子中HSV-1DNA,ELISA法检测患者血清中抗HSV-1的IgM、IgG抗体,并与正常人对照做比较。结果 患者皮损、PBMCs和咽拭子中HSV-1DNA检出率分别为37.5%、18.6%和18.8%,血清中抗HSV-1的IgM、IgG抗体检出率分别为37.2%和53.5%.经χ²检验,患者皮损、PBMCs中HSV-1DNA和血清中IgM抗体检出率显着高于正常人对照,点滴状患者的皮损、PBMCs和咽拭子中HSV-1DNA以及血清中抗HSV-1IgM抗体检出率显着高于斑块状患者。结论 寻常型银屑病尤其是皮损呈点滴状者与HSV-1显着相关,患者可能存在HSV-1的近期感染。     

银屑病患者血清中链球菌M6蛋白抗体的检测

为进一步证实链球菌中M6蛋白和点滴型银屑病之间的关系,用分离提纯的M6蛋白对银屑病患者血清中的抗体进行检测研究。(1)对点滴型银屑病患者咽部培养获得的化脓性链球菌进行PCR检测,经检测确认含有M6蛋白基因表达的菌株进行分离提纯M6蛋白。(2)用提纯的M6蛋白使用ELISA方法分别对30例点滴型银屑病患者,30例斑块型银屑病患者和30名健康对照组进行血清中M6蛋白抗体进行检测。点滴型银屑病患者血清中M6蛋白的抗体OD值明显高于斑块型银屑病组和正常对照组(P<0.01),而斑块型银屑病组和正常对照组间无明显差异。本组研究结果显示,点滴型银屑病患者与链球菌体中的M6蛋白有明显相关性。     

Coeliac Disease-Associated Antibodies in Psoriasis

Background

The possible relationship between psoriasis and coeliac disease (CD) has been attributed to the common pathogenic mechanisms of the two diseases and the presence of antigliadin antibodies in patients has been reported to increase the incidence of CD.

Objective

The aim of this report was to study CD-associated antibodies serum antigliadin antibody immunoglobulin (Ig)A, IgG, anti-endomysial antibody IgA and anti-transglutaminase antibody IgA and to demonstrate whether there is an increase in the frequency of those markers of CD in patients with psoriasis.

Methods

Serum antigliadin antibody IgG and IgA, antiendomysial antibody IgA and anti-transglutaminase antibody IgA were studied in 37 (19 males) patients with psoriasis and 50 (23 males) healthy controls. Upper gastrointestinal endoscopy and duodenal biopsies were performed in patients with at least one positive marker.

Results

Antigliadin IgA was statistically higher in the psoriasis group than in the controls (p<0.05). Serological markers were found positive in 6 patients with psoriasis and 1 person from the control group. Upper gastrointestinal endoscopy was performed in all these persons, with biopsies collected from the duodenum. The diagnosis of CD was reported in only one patient with psoriasis following the pathological examination of the biopsies. Whereas one person of the control group was found to be positive for antigliadin antibody IgA, pathological examination of the duodenal biopsies obtain from this patient were found to be normal.

Conclusion

Antigliadin IgA prominently increases in patients diagnosed with psoriasis. Patients with psoriasis should be investigated for latent CD and should be followed up.     

Antideoxyribonuclease B titers in psoriasis

Psoriatic patients were examined for serologic evidence of streptococcal infections by using antideoxyribonuclease B (ADB), which has been shown to be a more sensitive screening tool than antistreptolysin O (ASO) for detecting such evidence in other clinical circumstances. Antistreptococcal antibody titers (ASO and ADB) of 71 patients with psoriasis vulgaris, 7 with guttate psoriasis, and 6 with erythrodermic psoriasis were compared with ASO and ADB antibody titers of 25 non-psoriatic dermatologic patients hospitalized at the same time and of an adult group that was used as a control for the test itself. Thirty-six of 71 patients with psoriasis vulgaris had elevated titers of ADB or ASO (41% vs. 15%). Compared with control values the ADB titers were significantly higher in patients with psoriasis vulgaris (P less than 0.02) and patients with guttate psoriasis (P less than 0.05). The ASO titers were significantly higher in patients with psoriasis vulgaris than in controls (P less than 0.04). Within the group with psoriasis vulgaris, ADB titers were significantly higher than ASO titers (P less than 0.01). Among patients with psoriasis vulgaris, histories of sudden flares of psoriasis and early recurrences of psoriasis after previous successful hospital treatment, family histories of psoriasis, and histories of potential streptococcal infections were more frequent in those with elevated antistreptococcal antibodies.     

Coeliac disease-associated antibodies correlate with psoriasis activity

BACKGROUND: Antigliadin antibodies (AGA) have been reported in patients with psoriasis. OBJECTIVES: To determine if AGA and other coeliac disease (CD)-associated antibodies correlate with clinical features and activity in patients with psoriasis. METHODS: Patients with psoriasis (n = 130) were investigated for serum IgG and IgA AGA, IgA antitransglutaminase antibody and IgA antiendomysial antibody. Disease characteristics and associated bowel and joint symptoms were determined. All patients were invited to undertake endoscopy with duodenal biopsy. RESULTS: A significantly higher proportion of patients with elevated CD-associated antibody levels was currently on or had previously required systemic immunosuppressants (methotrexate, ciclosporin or etretinate; P = 0.04) or psoralen plus ultraviolet A phototherapy (P = 0.03). One case of CD was diagnosed. CONCLUSIONS: The presence of CD-associated antibodies in psoriasis patients correlates with greater disease activity.     

Ustekinumab in severe complicated erythrodermic psoriasis: rapid clearing,safety, and sustained remission

Erythrodermic psoriasis is a severe type of psoriasis associated with comorbidities and high mortality. Patients with erythrodermic psoriasis need hospitalization and systemic treatment. Conventional drugs and biologic agents may not manage to control refractory and complicated erythrodermic psoriasis resulting from treatment failure. Ustekinumab, a human monoclonal antibody against interleukin‐12 and 23, seems to be an effective therapeutic option in erythrodermic psoriasis whenever other therapies have failed.     

Estimation of (IgA) anti-gliadin, anti-endomysium and tissue transglutaminase in the serum of patients with psoriasis

Studies have indicated an association between psoriasis and coeliac disease (CD), an immune-mediated gluten-dependent enteropathy; however, the precise relationship between psoriasis and CD remains controversial. We aimed to assess the prevalence of the CD-associated IgA antibodies antigliadin antibody (AGA), tissue transglutaminase (tTG) and antiendomysium antibody (EMA) in patients with psoriasis. In total, 41 patients with psoriasis and 41 healthy controls were enrolled in this study. Blood samples were taken from all participants, and screened for AGA, tTG and EMA. We found a significantly higher level of AGA in patients with psoriasis than in controls, but levels of tTG and EMA were not significant. There was also a significantly higher prevalence of AGA, tTG and EMA in the patient group (34.1%, 34.1% and 14.6%, respectively) than in the control group (2.4%, 22% and 4.9%, respectively). We conclude that the significantly high prevalence of AGA antibodies in patients with psoriasis supports the possibility of a link between psoriasis and gluten-sensitive enteropathies, especially CD.     

Complete remission of psoriasis following bevacizumab therapy for colon cancer

Bevacizumab is a monoclonal antibody against vascular endothelial growth factor (VEGF), which is used to treat several cancers. Currently, experience with anti-VEGF treatment for psoriasis is limited, and no published reports on this use exist. We describe a patient with metastatic colon cancer and psoriasis who experienced complete remission of psoriasis during treatment with bevacizumab and combination chemotherapy without any other treatment for psoriasis. These data suggest that bevacizumab may be a promising therapeutic agent for the treatment of psoriasis.     

银屑病患者粒-单核巨噬细胞集落刺激因子表达的研究

目的 探讨脓疱性银屑病和寻常性银屑病患者皮损组织及外周血粒-单核巨噬细胞集落刺激因子（GM-CSF）表达的变化。方法 免疫组化法和双抗体夹心酶联免疫吸附法（ELISA）分别检测脓疱性银屑病和寻常性银屑病患者皮损组织和外周血中GM-CSF表达水平,并与正常人进行比较。结果 与正常人对照组相比,脓疱性银屑病组和寻常性银屑病组皮损组织中GM-CSF表达均增高（P < 0.01）,且脓疱性银屑病组皮损组织中GM-CSF表达高于寻常性银屑病组（P < 0.01）。与正常人对照组相比,脓疱性银屑病组和寻常性银屑病组外周血中GM-CSF含量均升高（P < 0.01）,且脓疱性银屑病组外周血中GM-CSF水平高于寻常性银屑病组（P < 0.01）。结论 GM-CSF可能参与银屑病的发病。     

New biologics for psoriasis and psoriatic arthritis

The prevalence of psoriasis is estimated to be 2.2% in the United States, and 6–39% of patients with psoriasis also develop psoriatic arthritis. New advances have been made in developing treatment options. A new human tumor necrosis factor (TNF)-α antibody, golimumab, has been shown to significantly improve symptoms of psoriatic arthritis. In addition, clinical trials of certolizumab pegol, a PEGylated Fab' fragment of an anti-TNF-α monoclonal antibody, show promising results for treating rheumatoid arthritis and suggest that it may be applicable for treating psoriasis and psoriatic arthritis in the future. New biologic therapies also include antibodies to interleukin-12 and interleukin-23. Phase II studies suggest that ustekinumab is effective in alleviating symptoms of psoriasis and psoriatic arthritis. However, longer studies with radiographic evaluation will be required before their impact on joint destruction can be assessed.     

Chemerin在寻常性银屑病患者血清中的表达水平及意义

目的 探讨Chemerin在寻常性银屑病患者血清中的表达水平及其临床意义.方法 采用双抗体夹心酶联免疫吸附法（Enzyme-linked immunosorbent assay,ELISA）检测30例寻常性银屑病患者血清Chemerin的浓度.另取30例正常人血清作为对照组.结果 寻常性银屑病患者血清中Chemerin浓度明显高于对照组（P＜0.01）,Chemerin的水平与寻常性银屑病的分期有关,其中进行期患者组显著高于静止期患者组（P＜0.05）,且Chemerin的血清浓度变化与寻常性银屑病严重程度指数PASI评分呈正相关性（r=0.521,P＜0.05）.结论 Chemerin在寻常性银屑病患者血清中显著增高,并与寻常性银屑病的分期相关,提示Chemerin在寻常性银屑病的发病中起重要作用。